Restriction map for
pFN21AE1886
(Circular) (Six-base) MAPSORT of: pFN21AE1886.seq Check: 4452 from: 1 to: 5941
pFN21AE1886 5941 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3751 278
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3284 1937
Size: 1347 4594
Acc65I G'GTAC_C
Cuts at: 3262 3262
Size: 5941
AclI AA'CG_TT
Cuts at: 1563 4068 4441 1563
Size: 2505 373 3063
Fragments arranged by size:
3063 2505 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3135 4004 5388 5656 1632
Size: 1503 869 1384 268 1917
Fragments arranged by size:
1917 1503 1384 869 268
AfeI AGC'GCT
Cuts at: 2555 2555
Size: 5941
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5744
AflIII A'CryG_T
Cuts at: 1203 4840 5817 1203
Size: 3637 977 1327
Fragments arranged by size:
3637 1327 977
AgeI A'CCGG_T
Cuts at: 4772 4772
Size: 5941
AhdI GACnn_n'nnGTC
Cuts at: 4670 4670
Size: 5941
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5941
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2591 1184
Size: 1407 4534
AlwNI CAG_nnn'CTG
Cuts at: 2212 2344 2986 3151 3684 5256 2212
Size: 132 642 165 533 1572 2897
Fragments arranged by size:
2897 1572 642 533 165 132
ApaLI G'TGCA_C
Cuts at: 3998 5154 3998
Size: 1156 4785
ApoI r'AATT_y
Cuts at: 1493 2324 2453 2750 3250 3476 3628 5737 1493
Size: 831 129 297 500 226 152 2109 1697
Fragments arranged by size:
2109 1697 831 500 297 226 152 129
AseI AT'TA_AT
Cuts at: 160 1032 4495 160
Size: 872 3463 1606
Fragments arranged by size:
3463 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
AvaI C'yCGr_G
Cuts at: 1870 1943 2232 2875 3266 1870
Size: 73 289 643 391 4545
Fragments arranged by size:
4545 643 391 289 73
BamHI G'GATC_C
Cuts at: 3271 3638 3271
Size: 367 5574
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2470 2799 3262 4717 618
Size: 325 217 627 683 329 463 1455 1842
Fragments arranged by size:
1842 1455 683 627 463 329 325 217
BanII G_rGCy'C
Cuts at: 1391 2029 2514 3159 3260 1391
Size: 638 485 645 101 4072
Fragments arranged by size:
4072 645 638 485 101
BbeI G_GCGC'C
Cuts at: 2803 2803
Size: 5941
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4970
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3108 3142 4132 4166 1903
Size: 34 1171 34 990 34 3678
Fragments arranged by size:
3678 1171 990 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3833 5043 3833
Size: 1210 4731
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5941
BfrBI ATG'CAT
Cuts at: 2254 2254
Size: 5941
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4552 136
Size: 107 122 71 631 605 2880 1525
Fragments arranged by size:
2880 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2142 2791 5936 2142
Size: 649 3145 2147
Fragments arranged by size:
3145 2147 649
BlpI GC'TnA_GC
Cuts at: 3358 3358
Size: 5941
Bme1580I G_kGCm'C
Cuts at: 1790 2475 2591 3144 4002 5158 1790
Size: 685 116 553 858 1156 2573
Fragments arranged by size:
2573 1156 858 685 553 116
BmgBI CAC'GTC
Cuts at: 1318 2197 1318
Size: 879 5062
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3672 4630 442
Size: 416 53 83 399 24 2255 958 1753
Fragments arranged by size:
2255 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5941
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3223 3223
Size: 5941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2264 3173 4601 1239
Size: 1025 909 1428 2579
Fragments arranged by size:
2579 1428 1025 909
Bpu10I CC'TnA_GC
Cuts at: 2156 2175 3037 2156
Size: 19 862 5060
Fragments arranged by size:
5060 862 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2167 2789 4066 4931 5229 2167
Size: 622 1277 865 298 2879
Fragments arranged by size:
2879 1277 865 622 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4604 882
Size: 617 3105 2219
Fragments arranged by size:
3105 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3732 493
Size: 711 2528 2702
Fragments arranged by size:
2702 2528 711
BsaBI GATnn'nnATC
Cuts at: 2744 5935 2744
Size: 3191 2750
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2800 3748 4130 4765
Size: 53 83 186 936 1267 948 382 635
Cuts at: 4765 275
Size: 1451
Fragments arranged by size:
1451 1267 948 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4374 4772 5046 5193 5604 4374
Size: 398 274 147 411 4711
Fragments arranged by size:
4711 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5716
Fragments arranged by size:
5716 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2261 2954 1186
Size: 1075 693 4173
Fragments arranged by size:
4173 1075 693
BseYI C'CCAG_C
Cuts at: 2480 3088 5144 5640 2480
Size: 608 2056 496 2781
Fragments arranged by size:
2781 2056 608 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2260 3084 1163
Size: 573 524 824 4020
Fragments arranged by size:
4020 824 573 524
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3391 4152 5180 1540
Size: 275 186 1390 761 1028 2301
Fragments arranged by size:
2301 1390 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2355 2514 2541 4002 4087 5158 2355
Size: 159 27 1461 85 1071 3138
Fragments arranged by size:
3138 1461 1071 159 85 27
BsmI GAATG_Cn'
Cuts at: 2676 3452 3545 2676
Size: 776 93 5072
Fragments arranged by size:
5072 776 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2029 2355 2475 2514 2541 2591 3144
Size: 399 239 326 120 39 27 50 553
Cuts at: 3144 3159 3260 4002 4087 5158 1391
Size: 15 101 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 553 399 326 239 120
101 85 50 39 27 15
BspEI T'CCGG_A
Cuts at: 5604 5604
Size: 5941
BspHI T'CATG_A
Cuts at: 2844 3830 5560 2844
Size: 986 1730 3225
Fragments arranged by size:
3225 1730 986
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3296 844
Size: 2452 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3828 1394
Size: 429 2005 3507
Fragments arranged by size:
3507 2005 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2541 4436 4610 65
Size: 1183 1293 1895 174 1396
Fragments arranged by size:
1895 1396 1293 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2862 4585 4772 1700
Size: 110 1052 1723 187 2869
Fragments arranged by size:
2869 1723 1052 187 110
BsrGI T'GTAC_A
Cuts at: 96 3096 96
Size: 3000 2941
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5941
BssSI C'ACGA_G
Cuts at: 2732 4001 5013 2732
Size: 1269 1012 3660
Fragments arranged by size:
3660 1269 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5941
BstBI TT'CG_AA
Cuts at: 4745 4745
Size: 5941
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5941
BstXI CCAn_nnnn'nTGG
Cuts at: 2786 3217 2786
Size: 431 5510
BstYI r'GATC_y
Cuts at: 1972 2142 2520 2791 2983 3271 3638 4023 4040
Size: 170 378 271 192 288 367 385 17
Cuts at: 4040 5492 5578 5590 5936 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 378 367 346 288 271
192 170 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3012 3012
Size: 5941
BtgI C'CryG_G
Cuts at: 513 1065 2058 2779 2785 513
Size: 552 993 721 6 3669
Fragments arranged by size:
3669 993 721 552 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5202
Fragments arranged by size:
5202 683 56
BtsI GCAGTG_nn'
Cuts at: 2589 3067 3457 4251 4271 2589
Size: 478 390 794 20 4259
Fragments arranged by size:
4259 794 478 390 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5941
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5906
DraI TTT'AAA
Cuts at: 3245 3245
Size: 5941
DraIII CAC_nnn'GTG
Cuts at: 2737 2737
Size: 5941
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4948 809
Size: 507 3632 1802
Fragments arranged by size:
3632 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2101 2528 2782
Size: 54 1425 50 275 18 271 427 254
Cuts at: 2782 3388 4277 5906 8
Size: 606 889 1629 43
Fragments arranged by size:
1629 1425 889 606 427 275 271 254
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3388 1537
Size: 275 1576 4090
Fragments arranged by size:
4090 1576 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2111 2519 2641 3871 1353
Size: 758 408 122 1230 3423
Fragments arranged by size:
3423 1230 758 408 122
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2363 4528 4902 5048 387
Size: 1064 138 774 2165 374 146 1280
Fragments arranged by size:
2165 1280 1064 774 374 146 138
EcoICRI GAG'CTC
Cuts at: 2512 2512
Size: 5941
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2264 3135 3173 4004 4601 5388 5656
Size: 393 632 871 38 831 597 787 268
Cuts at: 5656 1239
Size: 1524
Fragments arranged by size:
1524 871 831 787 632 597 393 268
38
EcoO109I rG'GnC_Cy
Cuts at: 2209 3041 3148 3186 2209
Size: 832 107 38 4964
Fragments arranged by size:
4964 832 107 38
EcoRI G'AATT_C
Cuts at: 2453 3250 3628 2453
Size: 797 378 4766
Fragments arranged by size:
4766 797 378
EcoRV GAT'ATC
Cuts at: 2716 2716
Size: 5941
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2032 2032
Size: 5941
FspI TGC'GCA
Cuts at: 4447 4447
Size: 5941
HaeII r_GCGC'y
Cuts at: 1678 2557 2803 5088 1678
Size: 879 246 2285 2531
Fragments arranged by size:
2531 2285 879 246
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2588 2620 4582 4614 4656
Size: 32 135 32 1016 32 1962 32 42
Cuts at: 4656 4688 1373
Size: 32 2626
Fragments arranged by size:
2626 1962 1016 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3285 3531 669
Size: 988 281 1347 246 3079
Fragments arranged by size:
3079 1347 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5941
HpaI GTT'AAC
Cuts at: 3531 3531
Size: 5941
KasI G'GCGC_C
Cuts at: 2799 2799
Size: 5941
KpnI G_GTAC'C
Cuts at: 3266 3266
Size: 5941
MfeI C'AATT_G
Cuts at: 3540 3540
Size: 5941
MluI A'CGCG_T
Cuts at: 5817 5817
Size: 5941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2277 2679 3001 3234 5055 5239 1305
Size: 972 402 322 233 1821 184 2007
Fragments arranged by size:
2007 1821 972 402 322 233 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4119
Fragments arranged by size:
4119 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2784 3218 3899 4258 4417 518
Size: 648 843 775 434 681 359 159 2042
Fragments arranged by size:
2042 843 775 681 648 434 359 159
MspA1I CmG'CkG
Cuts at: 1606 3357 4034 5182 5427 1606
Size: 1751 677 1148 245 2120
Fragments arranged by size:
2120 1751 1148 677 245
NarI GG'CG_CC
Cuts at: 2800 2800
Size: 5941
NcoI C'CATG_G
Cuts at: 513 1065 2779 513
Size: 552 1714 3675
Fragments arranged by size:
3675 1714 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5941
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5941
NotI GC'GGCC_GC
Cuts at: 3388 3388
Size: 5941
NsiI A_TGCA'T
Cuts at: 2256 2256
Size: 5941
NspI r_CATG'y
Cuts at: 1132 1229 3299 4844 1132
Size: 97 2070 1545 2229
Fragments arranged by size:
2229 2070 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5941
PciI A'CATG_T
Cuts at: 4840 4840
Size: 5941
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2344 3151 1108
Size: 1236 807 3898
Fragments arranged by size:
3898 1236 807
PfoI T'CCnGG_A
Cuts at: 2718 2718
Size: 5941
PmeI GTTT'AAAC
Cuts at: 3245 3245
Size: 5941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4010 4042 5523 5555 4010
Size: 32 1481 32 4396
Fragments arranged by size:
4396 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2209 3148 3186 2209
Size: 939 38 4964
Fragments arranged by size:
4964 939 38
PshAI GACnn'nnGTC
Cuts at: 1613 2195 1613
Size: 582 5359
PsiI TTA'TAA
Cuts at: 3511 3511
Size: 5941
PstI C_TGCA'G
Cuts at: 830 3293 830
Size: 2463 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5941
SacI G_AGCT'C
Cuts at: 2514 2514
Size: 5941
SalI G'TCGA_C
Cuts at: 1936 3283 1936
Size: 1347 4594
SapI GCTCTTCn'nnn_
Cuts at: 2519 2519
Size: 5941
SbfI CC_TGCA'GG
Cuts at: 3293 3293
Size: 5941
ScaI AGT'ACT
Cuts at: 4189 5901 4189
Size: 1712 4229
SfcI C'TryA_G
Cuts at: 826 1045 3289 4424 5105 5296 826
Size: 219 2244 1135 681 191 1471
Fragments arranged by size:
2244 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2801 2801
Size: 5941
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5941
SmaI CCC'GGG
Cuts at: 1872 2234 2877 3268 1872
Size: 362 643 391 4545
Fragments arranged by size:
4545 643 391 362
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2146 2804 4045 4946 5208 820
Size: 197 926 203 658 1241 901 262 1553
Fragments arranged by size:
1553 1241 926 901 658 262 203 197
SnaBI TAC'GTA
Cuts at: 493 3732 493
Size: 3239 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5941
SphI G_CATG'C
Cuts at: 1132 3299 1132
Size: 2167 3774
SspI AAT'ATT
Cuts at: 5 52 3865 5
Size: 47 3813 2081
Fragments arranged by size:
3813 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5941
StyI C'CwwG_G
Cuts at: 513 1065 2091 2391 2779 3380 513
Size: 552 1026 300 388 601 3074
Fragments arranged by size:
3074 1026 601 552 388 300
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2294 3968 3985 4138 4323 4768 325
Size: 1255 714 1674 17 153 185 445 1498
Fragments arranged by size:
1674 1498 1255 714 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3096 3171 4187
Size: 274 80 33 51 1443 1119 75 1016
Cuts at: 4187 5899 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1119 1016 274 138 80 75
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5901 5933 5901
Size: 32 5909
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5941
XbaI T'CTAG_A
Cuts at: 2665 3277 2665
Size: 612 5329
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5941
XmaI C'CCGG_G
Cuts at: 1870 2232 2875 3266 1870
Size: 362 643 391 4545
Fragments arranged by size:
4545 643 391 362
XmnI GAAnn'nnTTC
Cuts at: 2727 4070 2727
Size: 1343 4598
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3749 276
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BsiWI
BsmBI BstZ17I EcoNI FseI FspAI NaeI NgoMIV NruI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SexAI SfiI SrfI SwaI XcmI