Restriction map for
pFN21AE1885
(Circular) (Six-base) MAPSORT of: pFN21AE1885.seq Check: 6647 from: 1 to: 5938
pFN21AE1885 5938 bp
With 182 enzymes: SgfI *
March 28, 2012 11:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3748 278
Size: 53 83 186 936 2212 2468
Fragments arranged by size:
2468 2212 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3281 1937
Size: 1344 4594
Acc65I G'GTAC_C
Cuts at: 3259 3259
Size: 5938
AclI AA'CG_TT
Cuts at: 1563 4065 4438 1563
Size: 2502 373 3063
Fragments arranged by size:
3063 2502 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2082 4001 5385 5653 1632
Size: 450 1919 1384 268 1917
Fragments arranged by size:
1919 1917 1384 450 268
AfeI AGC'GCT
Cuts at: 2167 2167
Size: 5938
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5741
AflIII A'CryG_T
Cuts at: 1203 4837 5814 1203
Size: 3634 977 1327
Fragments arranged by size:
3634 1327 977
AgeI A'CCGG_T
Cuts at: 4769 4769
Size: 5938
AhdI GACnn_n'nnGTC
Cuts at: 3007 4667 3007
Size: 1660 4278
AleI CACnn'nnGTG
Cuts at: 1166 2942 1166
Size: 1776 4162
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5938
AlwNI CAG_nnn'CTG
Cuts at: 3681 5253 3681
Size: 1572 4366
ApaLI G'TGCA_C
Cuts at: 2531 3995 5151 2531
Size: 1464 1156 3318
Fragments arranged by size:
3318 1464 1156
ApoI r'AATT_y
Cuts at: 1493 3247 3473 3625 5734 1493
Size: 1754 226 152 2109 1697
Fragments arranged by size:
2109 1754 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4492 160
Size: 872 3460 1606
Fragments arranged by size:
3460 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
AvaI C'yCGr_G
Cuts at: 1870 1943 2102 2198 2993 3168 3263 1870
Size: 73 159 96 795 175 95 4545
Fragments arranged by size:
4545 795 175 159 96 95 73
BamHI G'GATC_C
Cuts at: 2868 3268 3635 2868
Size: 400 367 5171
Fragments arranged by size:
5171 400 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2553 3126 3259 4714 618
Size: 325 217 627 766 573 133 1455 1842
Fragments arranged by size:
1842 1455 766 627 573 325 217 133
BanII G_rGCy'C
Cuts at: 1391 2179 2304 3257 1391
Size: 788 125 953 4072
Fragments arranged by size:
4072 953 788 125
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3067 928
Size: 971 1168 3799
Fragments arranged by size:
3799 1168 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4129 4163 1903
Size: 34 2192 34 3678
Fragments arranged by size:
3678 2192 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3830 5040 3830
Size: 1210 4728
BclI T'GATC_A
Cuts at: 1548 2835 1548
Size: 1287 4651
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2399 4549 136
Size: 107 122 71 631 605 727 2150 1525
Fragments arranged by size:
2150 1525 727 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5933 5933
Size: 5938
BlpI GC'TnA_GC
Cuts at: 3106 3355 3106
Size: 249 5689
Bme1580I G_kGCm'C
Cuts at: 1790 2198 2535 3999 5155 1790
Size: 408 337 1464 1156 2573
Fragments arranged by size:
2573 1464 1156 408 337
BmgBI CAC'GTC
Cuts at: 1318 2268 1318
Size: 950 4988
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3669 4627 442
Size: 416 53 83 399 24 2252 958 1753
Fragments arranged by size:
2252 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5938
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3121 3121
Size: 5938
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 4598 1239
Size: 815 2544 2579
Fragments arranged by size:
2579 2544 815
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4063 4928 5226 4063
Size: 865 298 4775
Fragments arranged by size:
4775 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4601 882
Size: 617 3102 2219
Fragments arranged by size:
3102 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3729 493
Size: 711 2525 2702
Fragments arranged by size:
2702 2525 711
BsaBI GATnn'nnATC
Cuts at: 5932 5932
Size: 5938
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2214 3745 4127 4762
Size: 53 83 186 936 681 1531 382 635
Cuts at: 4762 275
Size: 1451
Fragments arranged by size:
1531 1451 936 681 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4371 4769 5043 5190 5601 4371
Size: 398 274 147 411 4708
Fragments arranged by size:
4708 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2166 2177 2196 2207 3131
Size: 30 165 30 763 11 19 11 924
Cuts at: 3131 3161 1178
Size: 30 3955
Fragments arranged by size:
3955 924 763 165 30 30 30 19
11 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5938
BseYI C'CCAG_C
Cuts at: 2082 5141 5637 2082
Size: 3059 496 2383
Fragments arranged by size:
3059 2383 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2321 2615 1163
Size: 573 585 294 4486
Fragments arranged by size:
4486 585 573 294
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3231 3388 4149 5177 1540
Size: 275 186 1230 157 761 1028 2301
Fragments arranged by size:
2301 1230 1028 761 275 186 157
BsiHKAI G_wGCw'C
Cuts at: 2535 2832 2949 3999 4084 5155 2535
Size: 297 117 1050 85 1071 3318
Fragments arranged by size:
3318 1071 1050 297 117 85
BsmI GAATG_Cn'
Cuts at: 2242 2255 2433 3449 3542 2242
Size: 13 178 1016 93 4638
Fragments arranged by size:
4638 1016 178 93 13
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2179 2198 2304 2535 2832 2949 3257
Size: 399 389 19 106 231 297 117 308
Cuts at: 3257 3999 4084 5155 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 389 308 297 231
117 106 85 19
BspEI T'CCGG_A
Cuts at: 5601 5601
Size: 5938
BspHI T'CATG_A
Cuts at: 2646 3827 5557 2646
Size: 1181 1730 3027
Fragments arranged by size:
3027 1730 1181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2343 2637 3293 844
Size: 1499 294 656 3489
Fragments arranged by size:
3489 1499 656 294
BsrBI CCG'CTC
Cuts at: 1394 1823 3825 1394
Size: 429 2002 3507
Fragments arranged by size:
3507 2002 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2548 4433 4607 65
Size: 1183 1300 1885 174 1396
Fragments arranged by size:
1885 1396 1300 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4582 4769 1700
Size: 110 2772 187 2869
Fragments arranged by size:
2869 2772 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5938
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5938
BssSI C'ACGA_G
Cuts at: 2180 2534 2825 3998 5010 2180
Size: 354 291 1173 1012 3108
Fragments arranged by size:
3108 1173 1012 354 291
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5938
BstBI TT'CG_AA
Cuts at: 4742 4742
Size: 5938
BstEII G'GTnAC_C
Cuts at: 1184 2590 1184
Size: 1406 4532
BstYI r'GATC_y
Cuts at: 1972 2868 3268 3635 4020 4037 5489 5575 5587
Size: 896 400 367 385 17 1452 86 12
Cuts at: 5587 5933 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 896 400 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3162 513
Size: 552 939 1158 3289
Fragments arranged by size:
3289 1158 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2332 487
Size: 683 56 1106 4093
Fragments arranged by size:
4093 1106 683 56
BtsI GCAGTG_nn'
Cuts at: 2015 2374 2934 3454 4248 4268 2015
Size: 359 560 520 794 20 3685
Fragments arranged by size:
3685 794 560 520 359 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5938
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5903
DraI TTT'AAA
Cuts at: 3242 3242
Size: 5938
DraIII CAC_nnn'GTG
Cuts at: 2297 2297
Size: 5938
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4945 809
Size: 507 3629 1802
Fragments arranged by size:
3629 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2400 2637 3228
Size: 54 1425 50 275 18 570 237 591
Cuts at: 3228 3385 4274 5903 8
Size: 157 889 1629 43
Fragments arranged by size:
1629 1425 889 591 570 275 237 157
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3228 3385 1537
Size: 275 1416 157 4090
Fragments arranged by size:
4090 1416 275 157
EarI CTCTTCn'nnn_
Cuts at: 1353 2058 2687 2693 2984 3113 3868 1353
Size: 705 629 6 291 129 755 3423
Fragments arranged by size:
3423 755 705 629 291 129 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4525 4899 5045 387
Size: 1064 138 2936 374 146 1280
Fragments arranged by size:
2936 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2082 4001 4598 5385 5653 1239
Size: 393 422 28 1919 597 787 268 1524
Fragments arranged by size:
1919 1524 787 597 422 393 268 28
EcoNI CCTnn'n_nnAGG
Cuts at: 2033 2033
Size: 5938
EcoO109I rG'GnC_Cy
Cuts at: 2108 2813 2108
Size: 705 5233
EcoRI G'AATT_C
Cuts at: 3247 3625 3247
Size: 378 5560
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2698 2698
Size: 5938
FspI TGC'GCA
Cuts at: 2672 4444 2672
Size: 1772 4166
HaeII r_GCGC'y
Cuts at: 1678 2057 2169 2668 5085 1678
Size: 379 112 499 2417 2531
Fragments arranged by size:
2531 2417 499 379 112
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2688 2720 2993 3025 3131
Size: 32 135 32 1116 32 273 32 106
Cuts at: 3131 3163 4579 4611 4653 4685 1373
Size: 32 1416 32 42 32 2626
Fragments arranged by size:
2626 1416 1116 273 135 106 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2362 2521 3282 3528 669
Size: 988 281 424 159 761 246 3079
Fragments arranged by size:
3079 988 761 424 281 246 159
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5938
HpaI GTT'AAC
Cuts at: 3528 3528
Size: 5938
KpnI G_GTAC'C
Cuts at: 3263 3263
Size: 5938
MfeI C'AATT_G
Cuts at: 3537 3537
Size: 5938
MluI A'CGCG_T
Cuts at: 5814 5814
Size: 5938
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5052 5236 1305
Size: 3747 184 2007
Fragments arranged by size:
3747 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4116
Fragments arranged by size:
4116 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2645 2825 2942 2972 3896 4255 4414
Size: 648 1479 180 117 30 924 359 159
Cuts at: 4414 518
Size: 2042
Fragments arranged by size:
2042 1479 924 648 359 180 159 117
30
MspA1I CmG'CkG
Cuts at: 1606 2559 3132 3233 3354 4031 5179 5424 1606
Size: 953 573 101 121 677 1148 245 2120
Fragments arranged by size:
2120 1148 953 677 573 245 121 101
NcoI C'CATG_G
Cuts at: 513 1065 2004 3162 513
Size: 552 939 1158 3289
Fragments arranged by size:
3289 1158 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5938
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5938
NotI GC'GGCC_GC
Cuts at: 3385 3385
Size: 5938
NspI r_CATG'y
Cuts at: 1132 1229 2977 3296 4841 1132
Size: 97 1748 319 1545 2229
Fragments arranged by size:
2229 1748 1545 319 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5938
PciI A'CATG_T
Cuts at: 4837 4837
Size: 5938
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5938
PmeI GTTT'AAAC
Cuts at: 3242 3242
Size: 5938
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4007 4039 5520 5552 4007
Size: 32 1481 32 4393
Fragments arranged by size:
4393 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2108 2108
Size: 5938
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5938
PsiI TTA'TAA
Cuts at: 3508 3508
Size: 5938
PstI C_TGCA'G
Cuts at: 830 2048 2514 3048 3290 830
Size: 1218 466 534 242 3478
Fragments arranged by size:
3478 1218 534 466 242
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5938
SalI G'TCGA_C
Cuts at: 1936 3280 1936
Size: 1344 4594
SanDI GG'GwC_CC
Cuts at: 2108 2108
Size: 5938
SapI GCTCTTCn'nnn_
Cuts at: 2058 2984 2058
Size: 926 5012
SbfI CC_TGCA'GG
Cuts at: 3290 3290
Size: 5938
ScaI AGT'ACT
Cuts at: 2204 4186 5898 2204
Size: 1982 1712 2244
Fragments arranged by size:
2244 1982 1712
SexAI A'CCwGG_T
Cuts at: 2790 2790
Size: 5938
SfcI C'TryA_G
Cuts at: 826 1045 2044 2510 3044 3286 4421 5102 5293
Size: 219 999 466 534 242 1135 681 191
Cuts at: 5293 826
Size: 1471
Fragments arranged by size:
1471 1135 999 681 534 466 242 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5938
SmaI CCC'GGG
Cuts at: 1872 2995 3170 3265 1872
Size: 1123 175 95 4545
Fragments arranged by size:
4545 1123 175 95
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4042 4943 5205 820
Size: 197 926 2099 901 262 1553
Fragments arranged by size:
2099 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3729 493
Size: 3236 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5938
SphI G_CATG'C
Cuts at: 1132 3296 1132
Size: 2164 3774
SrfI GCCC'GGGC
Cuts at: 3170 3170
Size: 5938
SspI AAT'ATT
Cuts at: 5 52 3862 5
Size: 47 3810 2081
Fragments arranged by size:
3810 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5938
StyI C'CwwG_G
Cuts at: 513 1065 2004 2133 2809 3162 3377 513
Size: 552 939 129 676 353 215 3074
Fragments arranged by size:
3074 939 676 552 353 215 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3208 3965 3982 4135 4320 4765 325
Size: 1255 1628 757 17 153 185 445 1498
Fragments arranged by size:
1628 1498 1255 757 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2202 2220 2916
Size: 274 80 33 51 1443 225 18 696
Cuts at: 2916 4184 5896 96
Size: 1268 1712 138
Fragments arranged by size:
1712 1443 1268 696 274 225 138 80
51 33 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2166 2177 2198 2209 5898 5930 2166
Size: 11 21 11 3689 32 2174
Fragments arranged by size:
3689 2174 32 21 11 11
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5938
XbaI T'CTAG_A
Cuts at: 3274 3274
Size: 5938
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2047 2047
Size: 5938
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5938
XmaI C'CCGG_G
Cuts at: 1870 2993 3168 3263 1870
Size: 1123 175 95 4545
Fragments arranged by size:
4545 1123 175 95
XmnI GAAnn'nnTTC
Cuts at: 4067 4067
Size: 5938
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3746 276
Size: 53 83 186 936 2212 2468
Fragments arranged by size:
2468 2212 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SfiI SfoI SwaI