Restriction map for
pFN21AE1882
(Circular) (Six-base) MAPSORT of: pFN21AE1882.seq Check: 2336 from: 1 to: 5908
pFN21AE1882 5908 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3718 278
Size: 53 83 186 936 2182 2468
Fragments arranged by size:
2468 2182 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2758 3251 1937
Size: 821 493 4594
Fragments arranged by size:
4594 821 493
Acc65I G'GTAC_C
Cuts at: 3229 3229
Size: 5908
AclI AA'CG_TT
Cuts at: 1563 4035 4408 1563
Size: 2472 373 3063
Fragments arranged by size:
3063 2472 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2370 2459 3971 5355 5623 1632
Size: 738 89 1512 1384 268 1917
Fragments arranged by size:
1917 1512 1384 738 268 89
AfeI AGC'GCT
Cuts at: 2957 2957
Size: 5908
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5711
AflIII A'CryG_T
Cuts at: 1203 4807 5784 1203
Size: 3604 977 1327
Fragments arranged by size:
3604 1327 977
AgeI A'CCGG_T
Cuts at: 4739 4739
Size: 5908
AhdI GACnn_n'nnGTC
Cuts at: 4637 4637
Size: 5908
AleI CACnn'nnGTG
Cuts at: 1166 2129 1166
Size: 963 4945
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2469 3099 1184
Size: 1285 630 3993
Fragments arranged by size:
3993 1285 630
AlwNI CAG_nnn'CTG
Cuts at: 2449 2489 3052 3651 5223 2449
Size: 40 563 599 1572 3134
Fragments arranged by size:
3134 1572 599 563 40
ApaLI G'TGCA_C
Cuts at: 2930 3965 5121 2930
Size: 1035 1156 3717
Fragments arranged by size:
3717 1156 1035
ApoI r'AATT_y
Cuts at: 1493 3217 3443 3595 5704 1493
Size: 1724 226 152 2109 1697
Fragments arranged by size:
2109 1724 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4462 160
Size: 872 3430 1606
Fragments arranged by size:
3430 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5908
AvaI C'yCGr_G
Cuts at: 1870 1943 3233 1870
Size: 73 1290 4545
Fragments arranged by size:
4545 1290 73
BamHI G'GATC_C
Cuts at: 3238 3605 3238
Size: 367 5541
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2516 3229 4684 618
Size: 325 217 627 729 713 1455 1842
Fragments arranged by size:
1842 1455 729 713 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2966 3227 1391
Size: 1575 261 4072
Fragments arranged by size:
4072 1575 261
BbeI G_GCGC'C
Cuts at: 2520 2520
Size: 5908
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4937
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4099 4133 1903
Size: 34 2162 34 3678
Fragments arranged by size:
3678 2162 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3058 3800 5010 3058
Size: 742 1210 3956
Fragments arranged by size:
3956 1210 742
BclI T'GATC_A
Cuts at: 1548 2089 2725 1548
Size: 541 636 4731
Fragments arranged by size:
4731 636 541
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4519 136
Size: 107 122 71 631 605 2847 1525
Fragments arranged by size:
2847 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5903 5903
Size: 5908
BlpI GC'TnA_GC
Cuts at: 2526 3325 2526
Size: 799 5109
Bme1580I G_kGCm'C
Cuts at: 1790 2934 3969 5125 1790
Size: 1144 1035 1156 2573
Fragments arranged by size:
2573 1156 1144 1035
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5908
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2601 3639 4597
Size: 416 53 83 399 24 1184 1038 958
Cuts at: 4597 442
Size: 1753
Fragments arranged by size:
1753 1184 1038 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5908
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2789 2789
Size: 5908
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2274 2486 2726 2980 4568 1239
Size: 1035 212 240 254 1588 2579
Fragments arranged by size:
2579 1588 1035 254 240 212
Bpu10I CC'TnA_GC
Cuts at: 2643 2985 2643
Size: 342 5566
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2646 4033 4898 5196 2646
Size: 1387 865 298 3358
Fragments arranged by size:
3358 1387 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4571 882
Size: 617 3072 2219
Fragments arranged by size:
3072 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3699 493
Size: 711 2495 2702
Fragments arranged by size:
2702 2495 711
BsaBI GATnn'nnATC
Cuts at: 2248 5902 2248
Size: 3654 2254
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2517 3715 4097 4732
Size: 53 83 186 936 984 1198 382 635
Cuts at: 4732 275
Size: 1451
Fragments arranged by size:
1451 1198 984 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4341 4739 5013 5160 5571 4341
Size: 398 274 147 411 4678
Fragments arranged by size:
4678 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5683
Fragments arranged by size:
5683 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2126 2408 1186
Size: 940 282 4686
Fragments arranged by size:
4686 940 282
BseYI C'CCAG_C
Cuts at: 2301 2476 3072 3199 5111 5607 2301
Size: 175 596 127 1912 496 2602
Fragments arranged by size:
2602 1912 596 496 175 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3005 3014 3146 1163
Size: 573 1269 9 132 3925
Fragments arranged by size:
3925 1269 573 132 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3358 4119 5147 1540
Size: 275 186 1357 761 1028 2301
Fragments arranged by size:
2301 1357 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2934 3969 4054 5125 2934
Size: 1035 85 1071 3717
Fragments arranged by size:
3717 1071 1035 85
BsmI GAATG_Cn'
Cuts at: 3419 3512 3419
Size: 93 5815
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2934 2966 3227 3969 4054 5125 1391
Size: 399 1144 32 261 742 85 1071 2174
Fragments arranged by size:
2174 1144 1071 742 399 261 85 32
BspEI T'CCGG_A
Cuts at: 5571 5571
Size: 5908
BspHI T'CATG_A
Cuts at: 3797 5527 3797
Size: 1730 4178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2744 3263 844
Size: 1900 519 3489
Fragments arranged by size:
3489 1900 519
BsrBI CCG'CTC
Cuts at: 1394 1823 3795 1394
Size: 429 1972 3507
Fragments arranged by size:
3507 1972 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2037 2967 4403 4577 65
Size: 1183 789 930 1436 174 1396
Fragments arranged by size:
1436 1396 1183 930 789 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4552 4739 1700
Size: 110 2742 187 2869
Fragments arranged by size:
2869 2742 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5908
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5908
BssSI C'ACGA_G
Cuts at: 3023 3968 4980 3023
Size: 945 1012 3951
Fragments arranged by size:
3951 1012 945
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3034 794
Size: 2240 3668
BstBI TT'CG_AA
Cuts at: 4712 4712
Size: 5908
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5908
BstYI r'GATC_y
Cuts at: 1972 3238 3605 3990 4007 5459 5545 5557 5903
Size: 1266 367 385 17 1452 86 12 346
Cuts at: 5903 1972
Size: 1977
Fragments arranged by size:
1977 1452 1266 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2082 2082
Size: 5908
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4417
Fragments arranged by size:
4417 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5169
Fragments arranged by size:
5169 683 56
BtsI GCAGTG_nn'
Cuts at: 3424 4218 4238 3424
Size: 794 20 5094
Fragments arranged by size:
5094 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5908
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5873
DraI TTT'AAA
Cuts at: 3212 3212
Size: 5908
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4915 809
Size: 507 3599 1802
Fragments arranged by size:
3599 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3150 3355 4244
Size: 54 1425 50 275 18 1320 205 889
Cuts at: 4244 5873 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1320 889 275 205 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3355 1537
Size: 275 1543 4090
Fragments arranged by size:
4090 1543 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3838 1353
Size: 2485 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4495 4869 5015 387
Size: 1064 138 435 2471 374 146 1280
Fragments arranged by size:
2471 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2274 2370 2459 2486 2726 2980 3971
Size: 393 642 96 89 27 240 254 991
Cuts at: 3971 4568 5355 5623 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 991 787 642 597 393 268 254
240 96 89 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2081 2867 2081
Size: 786 5122
EcoO109I rG'GnC_Cy
Cuts at: 2595 2595
Size: 5908
EcoRI G'AATT_C
Cuts at: 3217 3595 3217
Size: 378 5530
FspI TGC'GCA
Cuts at: 4414 4414
Size: 5908
HaeII r_GCGC'y
Cuts at: 1678 2320 2520 2533 2695 2814 2959 5055 1678
Size: 642 200 13 162 119 145 2096 2531
Fragments arranged by size:
2531 2096 642 200 162 145 119 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2642 2674 4549 4581 4623
Size: 32 135 32 1070 32 1875 32 42
Cuts at: 4623 4655 1373
Size: 32 2626
Fragments arranged by size:
2626 1875 1070 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3252 3498 669
Size: 988 281 1314 246 3079
Fragments arranged by size:
3079 1314 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5908
HpaI GTT'AAC
Cuts at: 3498 3498
Size: 5908
KasI G'GCGC_C
Cuts at: 2516 2516
Size: 5908
KpnI G_GTAC'C
Cuts at: 3233 3233
Size: 5908
MfeI C'AATT_G
Cuts at: 3507 3507
Size: 5908
MluI A'CGCG_T
Cuts at: 5784 5784
Size: 5908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5022 5206 1305
Size: 3717 184 2007
Fragments arranged by size:
3717 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3152 10
Size: 54 1425 343 1320 2766
Fragments arranged by size:
2766 1425 1320 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2129 3866 4225 4384 518
Size: 648 963 1737 359 159 2042
Fragments arranged by size:
2042 1737 963 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2428 2449 2545 2714 3019 3324 4001 5149
Size: 822 21 96 169 305 305 677 1148
Cuts at: 5149 5394 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 822 677 305 305 245 169
96 21
NarI GG'CG_CC
Cuts at: 2517 2517
Size: 5908
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4417
Fragments arranged by size:
4417 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5908
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5908
NotI GC'GGCC_GC
Cuts at: 3355 3355
Size: 5908
NspI r_CATG'y
Cuts at: 1132 1229 3266 4811 1132
Size: 97 2037 1545 2229
Fragments arranged by size:
2229 2037 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5908
PciI A'CATG_T
Cuts at: 4807 4807
Size: 5908
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5908
PmeI GTTT'AAAC
Cuts at: 3212 3212
Size: 5908
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3977 4009 5490 5522 3977
Size: 32 1481 32 4363
Fragments arranged by size:
4363 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2595 2595
Size: 5908
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5908
PsiI TTA'TAA
Cuts at: 3478 3478
Size: 5908
PstI C_TGCA'G
Cuts at: 830 2544 2755 3260 830
Size: 1714 211 505 3478
Fragments arranged by size:
3478 1714 505 211
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5908
PvuII CAG'CTG
Cuts at: 2428 2449 2545 2714 3019 2428
Size: 21 96 169 305 5317
Fragments arranged by size:
5317 305 169 96 21
SalI G'TCGA_C
Cuts at: 1936 3250 1936
Size: 1314 4594
SanDI GG'GwC_CC
Cuts at: 2595 2595
Size: 5908
SbfI CC_TGCA'GG
Cuts at: 3260 3260
Size: 5908
ScaI AGT'ACT
Cuts at: 4156 5868 4156
Size: 1712 4196
SfcI C'TryA_G
Cuts at: 826 1045 2540 2751 2759 2784 2852 2901 3256
Size: 219 1495 211 8 25 68 49 355
Cuts at: 3256 4391 5072 5263 826
Size: 1135 681 191 1471
Fragments arranged by size:
1495 1471 1135 681 355 219 211 191
68 49 25 8
SfoI GGC'GCC
Cuts at: 2518 2518
Size: 5908
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5908
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5908
SmaI CCC'GGG
Cuts at: 1872 3235 1872
Size: 1363 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2661 4012 4913 5175 820
Size: 197 926 718 1351 901 262 1553
Fragments arranged by size:
1553 1351 926 901 718 262 197
SnaBI TAC'GTA
Cuts at: 493 3699 493
Size: 3206 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5908
SphI G_CATG'C
Cuts at: 1132 3266 1132
Size: 2134 3774
SspI AAT'ATT
Cuts at: 5 52 3832 5
Size: 47 3780 2081
Fragments arranged by size:
3780 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5908
StyI C'CwwG_G
Cuts at: 513 1065 2004 3347 513
Size: 552 939 1343 3074
Fragments arranged by size:
3074 1343 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3086 3935 3952 4105 4290 4735 325
Size: 1255 1506 849 17 153 185 445 1498
Fragments arranged by size:
1506 1498 1255 849 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4154 5866 96
Size: 274 80 33 51 1443 2177 1712 138
Fragments arranged by size:
2177 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5868 5900 5868
Size: 32 5876
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5908
XbaI T'CTAG_A
Cuts at: 3244 3244
Size: 5908
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5908
XmaI C'CCGG_G
Cuts at: 1870 3233 1870
Size: 1363 4545
XmnI GAAnn'nnTTC
Cuts at: 4037 4037
Size: 5908
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3716 276
Size: 53 83 186 936 2182 2468
Fragments arranged by size:
2468 2182 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI
SexAI SfiI SrfI SwaI XcmI