Restriction map for
pFN21AE1881
(Circular) (Six-base) MAPSORT of: pFN21AE1881.seq Check: 8462 from: 1 to: 5923
pFN21AE1881 5923 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3733 278
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3266 1937
Size: 1329 4594
Acc65I G'GTAC_C
Cuts at: 3244 3244
Size: 5923
AclI AA'CG_TT
Cuts at: 1563 2050 4050 4423 1563
Size: 487 2000 373 3063
Fragments arranged by size:
3063 2000 487 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2163 2430 2961 3986 5370 5638 1632
Size: 531 267 531 1025 1384 268 1917
Fragments arranged by size:
1917 1384 1025 531 531 268 267
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5726
AflIII A'CryG_T
Cuts at: 1203 2491 4822 5799 1203
Size: 1288 2331 977 1327
Fragments arranged by size:
2331 1327 1288 977
AgeI A'CCGG_T
Cuts at: 4754 4754
Size: 5923
AhdI GACnn_n'nnGTC
Cuts at: 4652 4652
Size: 5923
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5923
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2575 1184
Size: 1391 4532
AlwNI CAG_nnn'CTG
Cuts at: 3184 3666 5238 3184
Size: 482 1572 3869
Fragments arranged by size:
3869 1572 482
ApaLI G'TGCA_C
Cuts at: 3980 5136 3980
Size: 1156 4767
ApoI r'AATT_y
Cuts at: 1493 2801 3209 3232 3458 3610 5719 1493
Size: 1308 408 23 226 152 2109 1697
Fragments arranged by size:
2109 1697 1308 408 226 152 23
AseI AT'TA_AT
Cuts at: 160 1032 4477 160
Size: 872 3445 1606
Fragments arranged by size:
3445 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
AvaI C'yCGr_G
Cuts at: 1870 1943 3248 1870
Size: 73 1305 4545
Fragments arranged by size:
4545 1305 73
BamHI G'GATC_C
Cuts at: 3253 3620 3253
Size: 367 5556
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3244 4699 618
Size: 325 217 627 1457 1455 1842
Fragments arranged by size:
1842 1457 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2916 3195 3242 1391
Size: 1525 279 47 4072
Fragments arranged by size:
4072 1525 279 47
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2250 928
Size: 971 351 4601
Fragments arranged by size:
4601 971 351
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4114 4148 1903
Size: 34 50 34 2093 34 3678
Fragments arranged by size:
3678 2093 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3815 5025 3815
Size: 1210 4713
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5923
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2864 4534 136
Size: 107 122 71 631 605 1192 1670 1525
Fragments arranged by size:
1670 1525 1192 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5918 5918
Size: 5923
BlpI GC'TnA_GC
Cuts at: 3340 3340
Size: 5923
Bme1580I G_kGCm'C
Cuts at: 1790 2595 3984 5140 1790
Size: 805 1389 1156 2573
Fragments arranged by size:
2573 1389 1156 805
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5923
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3067 3654 4612
Size: 416 53 83 399 24 1650 587 958
Cuts at: 4612 442
Size: 1753
Fragments arranged by size:
1753 1650 958 587 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5923
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2210 2210
Size: 5923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2082 2123 2273 4583 1239
Size: 843 41 150 2310 2579
Fragments arranged by size:
2579 2310 843 150 41
Bpu10I CC'TnA_GC
Cuts at: 2390 2390
Size: 5923
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4048 4913 5211 4048
Size: 865 298 4760
Fragments arranged by size:
4760 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4586 882
Size: 617 3087 2219
Fragments arranged by size:
3087 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2119 3714 493
Size: 711 915 1595 2702
Fragments arranged by size:
2702 1595 915 711
BsaBI GATnn'nnATC
Cuts at: 5917 5917
Size: 5923
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3730 4112 4747 275
Size: 53 83 186 936 2197 382 635 1451
Fragments arranged by size:
2197 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2534 4356 4754 5028 5175 5586 2534
Size: 1822 398 274 147 411 2871
Fragments arranged by size:
2871 1822 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2055 2076 2085 2106 1178
Size: 30 165 30 652 21 9 21 4995
Fragments arranged by size:
4995 652 165 30 30 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2379 2954 1186
Size: 1193 575 4155
Fragments arranged by size:
4155 1193 575
BseYI C'CCAG_C
Cuts at: 2994 3120 5126 5622 2994
Size: 126 2006 496 3295
Fragments arranged by size:
3295 2006 496 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2628 1163
Size: 573 892 4458
Fragments arranged by size:
4458 892 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3373 4134 5162 1540
Size: 275 186 1372 761 1028 2301
Fragments arranged by size:
2301 1372 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2160 3195 3984 4069 5140 2160
Size: 1035 789 85 1071 2943
Fragments arranged by size:
2943 1071 1035 789 85
BsmI GAATG_Cn'
Cuts at: 3434 3527 3434
Size: 93 5830
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2160 2595 2916 3195 3242 3984 4069
Size: 399 370 435 321 279 47 742 85
Cuts at: 4069 5140 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 435 399 370 321 279
85 47
BspEI T'CCGG_A
Cuts at: 5586 5586
Size: 5923
BspHI T'CATG_A
Cuts at: 2694 3812 5542 2694
Size: 1118 1730 3075
Fragments arranged by size:
3075 1730 1118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3278 844
Size: 2434 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3810 1394
Size: 429 1987 3507
Fragments arranged by size:
3507 1987 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4418 4592 65
Size: 1183 3170 174 1396
Fragments arranged by size:
3170 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3084 4567 4754 1700
Size: 110 1274 1483 187 2869
Fragments arranged by size:
2869 1483 1274 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5923
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5923
BssSI C'ACGA_G
Cuts at: 3983 4995 3983
Size: 1012 4911
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2590 794
Size: 1796 4127
BstBI TT'CG_AA
Cuts at: 4727 4727
Size: 5923
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5923
BstXI CCAn_nnnn'nTGG
Cuts at: 3120 3120
Size: 5923
BstYI r'GATC_y
Cuts at: 1972 3253 3620 4005 4022 5474 5560 5572 5918
Size: 1281 367 385 17 1452 86 12 346
Cuts at: 5918 1972
Size: 1977
Fragments arranged by size:
1977 1452 1281 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3079 3079
Size: 5923
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5371
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2305 487
Size: 683 56 1079 4105
Fragments arranged by size:
4105 1079 683 56
BtsI GCAGTG_nn'
Cuts at: 2616 3439 4233 4253 2616
Size: 823 794 20 4286
Fragments arranged by size:
4286 823 794 20
ClaI AT'CG_AT
Cuts at: 1554 2790 1554
Size: 1236 4687
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5888
DraI TTT'AAA
Cuts at: 2055 2679 3227 2055
Size: 624 548 4751
Fragments arranged by size:
4751 624 548
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4930 809
Size: 507 3614 1802
Fragments arranged by size:
3614 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3086 3370 4259
Size: 54 1425 50 275 18 1256 284 889
Cuts at: 4259 5888 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1256 889 284 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3370 1537
Size: 275 1558 4090
Fragments arranged by size:
4090 1558 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2102 3853 1353
Size: 749 1751 3423
Fragments arranged by size:
3423 1751 749
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4510 4884 5030 387
Size: 1064 138 2921 374 146 1280
Fragments arranged by size:
2921 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3193 3193
Size: 5923
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2082 2123 2163 2273 2430 2961 3986
Size: 393 450 41 40 110 157 531 1025
Cuts at: 3986 4583 5370 5638 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1025 787 597 531 450 393 268
157 110 41 40
EcoO109I rG'GnC_Cy
Cuts at: 2129 2129
Size: 5923
EcoRI G'AATT_C
Cuts at: 2801 3232 3610 2801
Size: 431 378 5114
Fragments arranged by size:
5114 431 378
EcoRV GAT'ATC
Cuts at: 2286 2286
Size: 5923
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2722 2722
Size: 5923
FseI GG_CCGG'CC
Cuts at: 3088 3088
Size: 5923
FspI TGC'GCA
Cuts at: 4429 4429
Size: 5923
HaeII r_GCGC'y
Cuts at: 1678 5070 1678
Size: 3392 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2076 2108 2270 2302 2622
Size: 32 135 32 504 32 162 32 320
Cuts at: 2622 2654 4564 4596 4638 4670 1373
Size: 32 1910 32 42 32 2626
Fragments arranged by size:
2626 1910 504 320 162 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3267 3513 669
Size: 988 281 1329 246 3079
Fragments arranged by size:
3079 1329 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5923
HpaI GTT'AAC
Cuts at: 3513 3513
Size: 5923
KpnI G_GTAC'C
Cuts at: 3248 3248
Size: 5923
MfeI C'AATT_G
Cuts at: 2931 3522 2931
Size: 591 5332
MluI A'CGCG_T
Cuts at: 5799 5799
Size: 5923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5037 5221 1305
Size: 3732 184 2007
Fragments arranged by size:
3732 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4101
Fragments arranged by size:
4101 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2645 3881 4240 4399 518
Size: 648 1479 1236 359 159 2042
Fragments arranged by size:
2042 1479 1236 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2994 3184 3339 4016 5164 5409 1606
Size: 1388 190 155 677 1148 245 2120
Fragments arranged by size:
2120 1388 1148 677 245 190 155
NaeI GCC'GGC
Cuts at: 3086 3086
Size: 5923
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5371
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5923
NgoMIV G'CCGG_C
Cuts at: 3084 3084
Size: 5923
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5923
NotI GC'GGCC_GC
Cuts at: 3370 3370
Size: 5923
NspI r_CATG'y
Cuts at: 1132 1229 3016 3281 4826 1132
Size: 97 1787 265 1545 2229
Fragments arranged by size:
2229 1787 1545 265 97
PasI CC'CwG_GG
Cuts at: 1348 3027 1348
Size: 1679 4244
PciI A'CATG_T
Cuts at: 4822 4822
Size: 5923
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2015 2749 2838 3027 1108
Size: 907 734 89 189 4004
Fragments arranged by size:
4004 907 734 189 89
PmeI GTTT'AAAC
Cuts at: 3227 3227
Size: 5923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3992 4024 5505 5537 3992
Size: 32 1481 32 4378
Fragments arranged by size:
4378 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2129 2129
Size: 5923
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5923
PsiI TTA'TAA
Cuts at: 3493 3493
Size: 5923
PstI C_TGCA'G
Cuts at: 830 3275 830
Size: 2445 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5923
PvuII CAG'CTG
Cuts at: 2994 3184 2994
Size: 190 5733
SacI G_AGCT'C
Cuts at: 3195 3195
Size: 5923
SalI G'TCGA_C
Cuts at: 1936 3265 1936
Size: 1329 4594
SbfI CC_TGCA'GG
Cuts at: 3275 3275
Size: 5923
ScaI AGT'ACT
Cuts at: 4171 5883 4171
Size: 1712 4211
SfcI C'TryA_G
Cuts at: 826 1045 3271 4406 5087 5278 826
Size: 219 2226 1135 681 191 1471
Fragments arranged by size:
2226 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5923
SmaI CCC'GGG
Cuts at: 1872 3250 1872
Size: 1378 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4027 4928 5190 820
Size: 197 926 2084 901 262 1553
Fragments arranged by size:
2084 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2119 3714 493
Size: 1626 1595 2702
Fragments arranged by size:
2702 1626 1595
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5923
SphI G_CATG'C
Cuts at: 1132 3281 1132
Size: 2149 3774
SspI AAT'ATT
Cuts at: 5 52 3847 5
Size: 47 3795 2081
Fragments arranged by size:
3795 2081 47
StuI AGG'CCT
Cuts at: 1517 2867 1517
Size: 1350 4573
StyI C'CwwG_G
Cuts at: 513 1065 2748 3362 513
Size: 552 1683 614 3074
Fragments arranged by size:
3074 1683 614 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2175 3950 3967 4120 4305 4750 325
Size: 1255 595 1775 17 153 185 445 1498
Fragments arranged by size:
1775 1498 1255 595 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4169 5881 96
Size: 274 80 33 51 1443 2192 1712 138
Fragments arranged by size:
2192 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2055 2087 5883 5915 2055
Size: 32 3796 32 2063
Fragments arranged by size:
3796 2063 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5923
XbaI T'CTAG_A
Cuts at: 3259 3259
Size: 5923
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5923
XmaI C'CCGG_G
Cuts at: 1870 3248 1870
Size: 1378 4545
XmnI GAAnn'nnTTC
Cuts at: 2169 4052 2169
Size: 1883 4040
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3731 276
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoNI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI