Restriction map for
pFN21AE1880
(Circular) (Six-base) MAPSORT of: pFN21AE1880.seq Check: 624 from: 1 to: 5923
pFN21AE1880 5923 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3062 3062
Size: 5923
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3733 278
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2199 3266 1937
Size: 262 1067 4594
Fragments arranged by size:
4594 1067 262
Acc65I G'GTAC_C
Cuts at: 3244 3244
Size: 5923
AclI AA'CG_TT
Cuts at: 1563 3222 4050 4423 1563
Size: 1659 828 373 3063
Fragments arranged by size:
3063 1659 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2912 3986 5370 5638 1632
Size: 1280 1074 1384 268 1917
Fragments arranged by size:
1917 1384 1280 1074 268
AflII C'TTAA_G
Cuts at: 820 1017 3127 820
Size: 197 2110 3616
Fragments arranged by size:
3616 2110 197
AflIII A'CryG_T
Cuts at: 1203 4822 5799 1203
Size: 3619 977 1327
Fragments arranged by size:
3619 1327 977
AgeI A'CCGG_T
Cuts at: 4754 4754
Size: 5923
AhdI GACnn_n'nnGTC
Cuts at: 4652 4652
Size: 5923
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5923
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5923
AlwNI CAG_nnn'CTG
Cuts at: 3666 5238 3666
Size: 1572 4351
ApaLI G'TGCA_C
Cuts at: 3980 5136 3980
Size: 1156 4767
ApoI r'AATT_y
Cuts at: 1493 2876 3232 3458 3610 5719 1493
Size: 1383 356 226 152 2109 1697
Fragments arranged by size:
2109 1697 1383 356 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4477 160
Size: 872 3445 1606
Fragments arranged by size:
3445 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
AvaI C'yCGr_G
Cuts at: 1870 1943 2114 2291 3248 1870
Size: 73 171 177 957 4545
Fragments arranged by size:
4545 957 177 171 73
BamHI G'GATC_C
Cuts at: 2487 3253 3620 2487
Size: 766 367 4790
Fragments arranged by size:
4790 766 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2567 3244 4699 618
Size: 325 217 627 780 677 1455 1842
Fragments arranged by size:
1842 1455 780 677 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2751 2984 3242 1391
Size: 1360 233 258 4072
Fragments arranged by size:
4072 1360 258 233
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2975 3054 3212 928
Size: 971 1076 79 158 3639
Fragments arranged by size:
3639 1076 971 158 79
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2129 2163 2306 2340 4114 4148 1903
Size: 34 192 34 143 34 1774 34 3678
Fragments arranged by size:
3678 1774 192 143 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3815 5025 3815
Size: 1210 4713
BclI T'GATC_A
Cuts at: 1548 2577 1548
Size: 1029 4894
BfrBI ATG'CAT
Cuts at: 2931 2931
Size: 5923
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4534 136
Size: 107 122 71 631 605 2862 1525
Fragments arranged by size:
2862 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5918 5918
Size: 5923
BlpI GC'TnA_GC
Cuts at: 3340 3340
Size: 5923
Bme1580I G_kGCm'C
Cuts at: 1790 2014 3984 5140 1790
Size: 224 1970 1156 2573
Fragments arranged by size:
2573 1970 1156 224
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5923
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2471 3129 3205
Size: 416 53 83 399 24 1054 658 76
Cuts at: 3205 3654 4612 442
Size: 449 958 1753
Fragments arranged by size:
1753 1054 958 658 449 416 399 83
76 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3116 4583 1239
Size: 1877 1467 2579
Fragments arranged by size:
2579 1877 1467
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4048 4913 5211 4048
Size: 865 298 4760
Fragments arranged by size:
4760 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4586 882
Size: 617 3087 2219
Fragments arranged by size:
3087 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3714 493
Size: 711 2510 2702
Fragments arranged by size:
2702 2510 711
BsaBI GATnn'nnATC
Cuts at: 2795 5917 2795
Size: 3122 2801
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3730 4112 4747 275
Size: 53 83 186 936 2197 382 635 1451
Fragments arranged by size:
2197 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4356 4754 5028 5175 5586 4356
Size: 398 274 147 411 4693
Fragments arranged by size:
4693 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2181 2211 2358 2388 1178
Size: 30 165 30 778 30 147 30 4713
Fragments arranged by size:
4713 778 165 147 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3113 1186
Size: 1927 3996
BseYI C'CCAG_C
Cuts at: 2853 5126 5622 2853
Size: 2273 496 3154
Fragments arranged by size:
3154 2273 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2501 3014 1163
Size: 573 765 513 4072
Fragments arranged by size:
4072 765 573 513
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3373 4134 5162 1540
Size: 275 186 1372 761 1028 2301
Fragments arranged by size:
2301 1372 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2142 2319 3984 4069 5140 2142
Size: 177 1665 85 1071 2925
Fragments arranged by size:
2925 1665 1071 177 85
BsmI GAATG_Cn'
Cuts at: 3434 3527 3434
Size: 93 5830
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2014 2142 2319 2751 2984 3242 3984
Size: 399 224 128 177 432 233 258 742
Cuts at: 3984 4069 5140 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 432 399 258 233 224
177 128 85
BspEI T'CCGG_A
Cuts at: 5586 5586
Size: 5923
BspHI T'CATG_A
Cuts at: 3812 5542 3812
Size: 1730 4193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3062 3278 844
Size: 2218 216 3489
Fragments arranged by size:
3489 2218 216
BsrBI CCG'CTC
Cuts at: 1394 1823 3810 1394
Size: 429 1987 3507
Fragments arranged by size:
3507 1987 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2025 2077 2652 4418 4592 65
Size: 1183 777 52 575 1766 174 1396
Fragments arranged by size:
1766 1396 1183 777 575 174 52
BsrFI r'CCGG_y
Cuts at: 1700 1810 4567 4754 1700
Size: 110 2757 187 2869
Fragments arranged by size:
2869 2757 187 110
BsrGI T'GTAC_A
Cuts at: 96 2376 96
Size: 2280 3643
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5923
BssSI C'ACGA_G
Cuts at: 3983 4995 3983
Size: 1012 4911
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5923
BstBI TT'CG_AA
Cuts at: 4727 4727
Size: 5923
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5923
BstYI r'GATC_y
Cuts at: 1972 2487 3123 3253 3620 4005 4022 5474 5560
Size: 515 636 130 367 385 17 1452 86
Cuts at: 5560 5572 5918 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 636 515 385 367 346 130
86 17 12
BstZ17I GTA'TAC
Cuts at: 2200 2200
Size: 5923
BtgI C'CryG_G
Cuts at: 513 1065 2601 513
Size: 552 1536 3835
Fragments arranged by size:
3835 1536 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3078 487
Size: 683 56 1852 3332
Fragments arranged by size:
3332 1852 683 56
BtsI GCAGTG_nn'
Cuts at: 2064 2228 2405 3079 3439 4233 4253 2064
Size: 164 177 674 360 794 20 3734
Fragments arranged by size:
3734 794 674 360 177 164 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5923
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5888
DraI TTT'AAA
Cuts at: 2875 3227 2875
Size: 352 5571
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4930 809
Size: 507 3614 1802
Fragments arranged by size:
3614 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3370 4259 5888
Size: 54 1425 50 275 18 1540 889 1629
Cuts at: 5888 8
Size: 43
Fragments arranged by size:
1629 1540 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3370 1537
Size: 275 1558 4090
Fragments arranged by size:
4090 1558 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2621 2972 3853 1353
Size: 1268 351 881 3423
Fragments arranged by size:
3423 1268 881 351
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4510 4884 5030 387
Size: 1064 138 2921 374 146 1280
Fragments arranged by size:
2921 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2912 3116 3986 4583 5370 5638 1239
Size: 393 1280 204 870 597 787 268 1524
Fragments arranged by size:
1524 1280 870 787 597 393 268 204
EcoNI CCTnn'n_nnAGG
Cuts at: 3098 3098
Size: 5923
EcoRI G'AATT_C
Cuts at: 3232 3610 3232
Size: 378 5545
EcoRV GAT'ATC
Cuts at: 2086 2086
Size: 5923
FspI TGC'GCA
Cuts at: 2180 2357 4429 2180
Size: 177 2072 3674
Fragments arranged by size:
3674 2072 177
HaeII r_GCGC'y
Cuts at: 1678 5070 1678
Size: 3392 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4564 4596 4638 4670 1373
Size: 32 135 32 2992 32 42 32 2626
Fragments arranged by size:
2992 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3267 3513 669
Size: 988 281 1329 246 3079
Fragments arranged by size:
3079 1329 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3130 748
Size: 2382 3541
HpaI GTT'AAC
Cuts at: 3513 3513
Size: 5923
KpnI G_GTAC'C
Cuts at: 3248 3248
Size: 5923
MfeI C'AATT_G
Cuts at: 3522 3522
Size: 5923
MluI A'CGCG_T
Cuts at: 5799 5799
Size: 5923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2221 2398 5037 5221 1305
Size: 916 177 2639 184 2007
Fragments arranged by size:
2639 2007 916 184 177
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4101
Fragments arranged by size:
4101 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2130 2307 2505 3881 4240 4399 518
Size: 648 964 177 198 1376 359 159 2042
Fragments arranged by size:
2042 1376 964 648 359 198 177 159
MspA1I CmG'CkG
Cuts at: 1606 2485 3339 4016 5164 5409 1606
Size: 879 854 677 1148 245 2120
Fragments arranged by size:
2120 1148 879 854 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5371
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5923
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5923
NotI GC'GGCC_GC
Cuts at: 3370 3370
Size: 5923
NsiI A_TGCA'T
Cuts at: 2933 2933
Size: 5923
NspI r_CATG'y
Cuts at: 1132 1229 3034 3281 4826 1132
Size: 97 1805 247 1545 2229
Fragments arranged by size:
2229 1805 1545 247 97
PasI CC'CwG_GG
Cuts at: 1348 2014 1348
Size: 666 5257
PciI A'CATG_T
Cuts at: 4822 4822
Size: 5923
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5923
PfoI T'CCnGG_A
Cuts at: 3189 3189
Size: 5923
PmeI GTTT'AAAC
Cuts at: 3227 3227
Size: 5923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3992 4024 5505 5537 3992
Size: 32 1481 32 4378
Fragments arranged by size:
4378 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5923
PsiI TTA'TAA
Cuts at: 3493 3493
Size: 5923
PstI C_TGCA'G
Cuts at: 830 3275 830
Size: 2445 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5923
SalI G'TCGA_C
Cuts at: 1936 3265 1936
Size: 1329 4594
SapI GCTCTTCn'nnn_
Cuts at: 2972 2972
Size: 5923
SbfI CC_TGCA'GG
Cuts at: 3275 3275
Size: 5923
ScaI AGT'ACT
Cuts at: 4171 5883 4171
Size: 1712 4211
SexAI A'CCwGG_T
Cuts at: 3107 3107
Size: 5923
SfcI C'TryA_G
Cuts at: 826 1045 2985 3271 4406 5087 5278 826
Size: 219 1940 286 1135 681 191 1471
Fragments arranged by size:
1940 1471 1135 681 286 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5923
SmaI CCC'GGG
Cuts at: 1872 3250 1872
Size: 1378 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3127 4027 4928 5190 820
Size: 197 926 1184 900 901 262 1553
Fragments arranged by size:
1553 1184 926 901 900 262 197
SnaBI TAC'GTA
Cuts at: 493 3714 493
Size: 3221 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5923
SphI G_CATG'C
Cuts at: 1132 3281 1132
Size: 2149 3774
SspI AAT'ATT
Cuts at: 5 52 3847 5
Size: 47 3795 2081
Fragments arranged by size:
3795 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5923
StyI C'CwwG_G
Cuts at: 513 1065 3362 513
Size: 552 2297 3074
Fragments arranged by size:
3074 2297 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2483 3950 3967 4120 4305 4750 325
Size: 1255 903 1467 17 153 185 445 1498
Fragments arranged by size:
1498 1467 1255 903 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2376 4169 5881
Size: 274 80 33 51 1443 399 1793 1712
Cuts at: 5881 96
Size: 138
Fragments arranged by size:
1793 1712 1443 399 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2358 2390 2479 2511 5883 5915 2358
Size: 32 89 32 3372 32 2366
Fragments arranged by size:
3372 2366 89 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5923
XbaI T'CTAG_A
Cuts at: 3259 3259
Size: 5923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2741 2011
Size: 730 5193
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5923
XmaI C'CCGG_G
Cuts at: 1870 3248 1870
Size: 1378 4545
XmnI GAAnn'nnTTC
Cuts at: 4052 4052
Size: 5923
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3731 276
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI
EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI