Restriction map for
pFN21AE1879
(Circular) (Six-base) MAPSORT of: pFN21AE1879.seq Check: 6682 from: 1 to: 5917
pFN21AE1879 5917 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3727 278
Size: 53 83 186 936 2191 2468
Fragments arranged by size:
2468 2191 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2469 3260 1937
Size: 532 791 4594
Fragments arranged by size:
4594 791 532
Acc65I G'GTAC_C
Cuts at: 2989 3238 2989
Size: 249 5668
AclI AA'CG_TT
Cuts at: 1563 4044 4417 1563
Size: 2481 373 3063
Fragments arranged by size:
3063 2481 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3053 3115 3980 5364 5632 1632
Size: 1421 62 865 1384 268 1917
Fragments arranged by size:
1917 1421 1384 865 268 62
AflII C'TTAA_G
Cuts at: 820 1017 2674 820
Size: 197 1657 4063
Fragments arranged by size:
4063 1657 197
AflIII A'CryG_T
Cuts at: 1203 2352 3214 4816 5793 1203
Size: 1149 862 1602 977 1327
Fragments arranged by size:
1602 1327 1149 977 862
AgeI A'CCGG_T
Cuts at: 4748 4748
Size: 5917
AhdI GACnn_n'nnGTC
Cuts at: 4646 4646
Size: 5917
AleI CACnn'nnGTG
Cuts at: 1166 3157 1166
Size: 1991 3926
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5917
AlwNI CAG_nnn'CTG
Cuts at: 3660 5232 3660
Size: 1572 4345
ApaLI G'TGCA_C
Cuts at: 3974 5130 3974
Size: 1156 4761
ApoI r'AATT_y
Cuts at: 1493 3226 3452 3604 5713 1493
Size: 1733 226 152 2109 1697
Fragments arranged by size:
2109 1733 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2295 4471 160
Size: 872 1263 2176 1606
Fragments arranged by size:
2176 1606 1263 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
AvaI C'yCGr_G
Cuts at: 1870 1943 3242 1870
Size: 73 1299 4545
Fragments arranged by size:
4545 1299 73
BamHI G'GATC_C
Cuts at: 3247 3614 3247
Size: 367 5550
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2989 3238 4693 618
Size: 325 217 627 1202 249 1455 1842
Fragments arranged by size:
1842 1455 1202 627 325 249 217
BanII G_rGCy'C
Cuts at: 1391 3236 1391
Size: 1845 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3041 3066 928
Size: 971 1142 25 3779
Fragments arranged by size:
3779 1142 971 25
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4108 4142 1903
Size: 34 2171 34 3678
Fragments arranged by size:
3678 2171 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3809 5019 3809
Size: 1210 4707
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5917
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4528 136
Size: 107 122 71 631 605 2856 1525
Fragments arranged by size:
2856 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2842 5912 2842
Size: 3070 2847
BlpI GC'TnA_GC
Cuts at: 2034 2832 3334 2034
Size: 798 502 4617
Fragments arranged by size:
4617 798 502
Bme1580I G_kGCm'C
Cuts at: 1790 3978 5134 1790
Size: 2188 1156 2573
Fragments arranged by size:
2573 2188 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5917
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3648 4606 442
Size: 416 53 83 399 24 2231 958 1753
Fragments arranged by size:
2231 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2242 3125 4577 1239
Size: 1003 883 1452 2579
Fragments arranged by size:
2579 1452 1003 883
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2621 4042 4907 5205 2621
Size: 1421 865 298 3333
Fragments arranged by size:
3333 1421 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4580 882
Size: 617 3081 2219
Fragments arranged by size:
3081 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3708 493
Size: 711 2504 2702
Fragments arranged by size:
2702 2504 711
BsaBI GATnn'nnATC
Cuts at: 5911 5911
Size: 5917
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3724 4106 4741 275
Size: 53 83 186 936 2191 382 635 1451
Fragments arranged by size:
2191 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4350 4748 5022 5169 5580 4350
Size: 398 274 147 411 4687
Fragments arranged by size:
4687 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5692
Fragments arranged by size:
5692 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2150 1186
Size: 964 4953
BseYI C'CCAG_C
Cuts at: 2046 2051 2322 5120 5616 2046
Size: 5 271 2798 496 2347
Fragments arranged by size:
2798 2347 496 271 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5344
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3367 4128 5156 1540
Size: 275 186 1366 761 1028 2301
Fragments arranged by size:
2301 1366 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3194 3978 4063 5134 3194
Size: 784 85 1071 3977
Fragments arranged by size:
3977 1071 784 85
BsmI GAATG_Cn'
Cuts at: 2404 3428 3521 2404
Size: 1024 93 4800
Fragments arranged by size:
4800 1024 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3194 3236 3978 4063 5134 1391
Size: 399 1404 42 742 85 1071 2174
Fragments arranged by size:
2174 1404 1071 742 399 85 42
BspEI T'CCGG_A
Cuts at: 5580 5580
Size: 5917
BspHI T'CATG_A
Cuts at: 2380 3806 5536 2380
Size: 1426 1730 2761
Fragments arranged by size:
2761 1730 1426
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3272 844
Size: 2428 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3804 1394
Size: 429 1981 3507
Fragments arranged by size:
3507 1981 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2586 3169 4412 4586 65
Size: 1183 1338 583 1243 174 1396
Fragments arranged by size:
1396 1338 1243 1183 583 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4561 4748 1700
Size: 110 2751 187 2869
Fragments arranged by size:
2869 2751 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5917
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5917
BssSI C'ACGA_G
Cuts at: 3977 4989 3977
Size: 1012 4905
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5917
BstBI TT'CG_AA
Cuts at: 4721 4721
Size: 5917
BstEII G'GTnAC_C
Cuts at: 1184 2140 1184
Size: 956 4961
BstXI CCAn_nnnn'nTGG
Cuts at: 2929 2929
Size: 5917
BstYI r'GATC_y
Cuts at: 1972 2842 3247 3614 3999 4016 5468 5554 5566
Size: 870 405 367 385 17 1452 86 12
Cuts at: 5566 5912 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 870 405 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2470 2470
Size: 5917
Bsu36I CC'TnA_GG
Cuts at: 2944 2944
Size: 5917
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5365
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5178
Fragments arranged by size:
5178 683 56
BtsI GCAGTG_nn'
Cuts at: 2665 3433 4227 4247 2665
Size: 768 794 20 4335
Fragments arranged by size:
4335 794 768 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5917
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5882
DraI TTT'AAA
Cuts at: 2281 2706 3221 2281
Size: 425 515 4977
Fragments arranged by size:
4977 515 425
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4924 809
Size: 507 3608 1802
Fragments arranged by size:
3608 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2177 3364 4253
Size: 54 1425 50 275 18 347 1187 889
Cuts at: 4253 5882 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1187 889 347 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3364 1537
Size: 275 1552 4090
Fragments arranged by size:
4090 1552 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2093 2125 2658 3182 3847 1353
Size: 740 32 533 524 665 3423
Fragments arranged by size:
3423 740 665 533 524 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4504 4878 5024 387
Size: 1064 138 2915 374 146 1280
Fragments arranged by size:
2915 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2242 3053 3115 3125 3980 4577 5364
Size: 393 610 811 62 10 855 597 787
Cuts at: 5364 5632 1239
Size: 268 1524
Fragments arranged by size:
1524 855 811 787 610 597 393 268
62 10
EcoRI G'AATT_C
Cuts at: 3226 3604 3226
Size: 378 5539
EcoRV GAT'ATC
Cuts at: 2671 2671
Size: 5917
FspI TGC'GCA
Cuts at: 4423 4423
Size: 5917
HaeII r_GCGC'y
Cuts at: 1678 5064 1678
Size: 3386 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2124 2156 3057 3089 4558
Size: 32 135 32 552 32 901 32 1469
Cuts at: 4558 4590 4632 4664 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1469 901 552 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2529 3261 3507 669
Size: 988 281 591 732 246 3079
Fragments arranged by size:
3079 988 732 591 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5917
HpaI GTT'AAC
Cuts at: 3507 3507
Size: 5917
KpnI G_GTAC'C
Cuts at: 2993 3242 2993
Size: 249 5668
MfeI C'AATT_G
Cuts at: 3516 3516
Size: 5917
MluI A'CGCG_T
Cuts at: 5793 5793
Size: 5917
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5031 5215 1305
Size: 3726 184 2007
Fragments arranged by size:
3726 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2179 10
Size: 54 1425 343 347 3748
Fragments arranged by size:
3748 1425 347 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2157 2927 3157 3875 4234 4393 518
Size: 648 991 770 230 718 359 159 2042
Fragments arranged by size:
2042 991 770 718 648 359 230 159
MspA1I CmG'CkG
Cuts at: 1606 3333 4010 5158 5403 1606
Size: 1727 677 1148 245 2120
Fragments arranged by size:
2120 1727 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5365
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5917
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5917
NotI GC'GGCC_GC
Cuts at: 3364 3364
Size: 5917
NspI r_CATG'y
Cuts at: 1132 1229 2356 3275 4820 1132
Size: 97 1127 919 1545 2229
Fragments arranged by size:
2229 1545 1127 919 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5917
PciI A'CATG_T
Cuts at: 2352 4816 2352
Size: 2464 3453
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5917
PmeI GTTT'AAAC
Cuts at: 3221 3221
Size: 5917
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3986 4018 5499 5531 3986
Size: 32 1481 32 4372
Fragments arranged by size:
4372 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3148 1613
Size: 1535 4382
PsiI TTA'TAA
Cuts at: 3487 3487
Size: 5917
PstI C_TGCA'G
Cuts at: 830 3269 830
Size: 2439 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5917
SalI G'TCGA_C
Cuts at: 1936 3259 1936
Size: 1323 4594
SapI GCTCTTCn'nnn_
Cuts at: 3182 3182
Size: 5917
SbfI CC_TGCA'GG
Cuts at: 3269 3269
Size: 5917
ScaI AGT'ACT
Cuts at: 3016 4165 5877 3016
Size: 1149 1712 3056
Fragments arranged by size:
3056 1712 1149
SfcI C'TryA_G
Cuts at: 826 1045 3265 4400 5081 5272 826
Size: 219 2220 1135 681 191 1471
Fragments arranged by size:
2220 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5917
SmaI CCC'GGG
Cuts at: 1872 3244 1872
Size: 1372 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2600 2674 4021 4922 5184 820
Size: 197 926 657 74 1347 901 262 1553
Fragments arranged by size:
1553 1347 926 901 657 262 197 74
SnaBI TAC'GTA
Cuts at: 493 3708 493
Size: 3215 2702
SpeI A'CTAG_T
Cuts at: 152 3017 152
Size: 2865 3052
SphI G_CATG'C
Cuts at: 1132 3275 1132
Size: 2143 3774
SspI AAT'ATT
Cuts at: 5 52 3841 5
Size: 47 3789 2081
Fragments arranged by size:
3789 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5917
StyI C'CwwG_G
Cuts at: 513 1065 3356 513
Size: 552 2291 3074
Fragments arranged by size:
3074 2291 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3944 3961 4114 4299 4744 325
Size: 1255 2364 17 153 185 445 1498
Fragments arranged by size:
2364 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2880 3014 4163
Size: 274 80 33 51 1443 903 134 1149
Cuts at: 4163 5875 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1149 903 274 138 134 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5877 5909 5877
Size: 32 5885
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5917
XbaI T'CTAG_A
Cuts at: 2440 3253 2440
Size: 813 5104
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5917
XmaI C'CCGG_G
Cuts at: 1870 3242 1870
Size: 1372 4545
XmnI GAAnn'nnTTC
Cuts at: 4046 4046
Size: 5917
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3725 276
Size: 53 83 186 936 2191 2468
Fragments arranged by size:
2468 2191 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI DraIII EcoICRI
EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI