Restriction map for
pFN21AE1878
(Circular) (Six-base) MAPSORT of: pFN21AE1878.seq Check: 5219 from: 1 to: 5899
pFN21AE1878 5899 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3709 278
Size: 53 83 186 936 2173 2468
Fragments arranged by size:
2468 2173 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3242 1937
Size: 1305 4594
Acc65I G'GTAC_C
Cuts at: 3220 3220
Size: 5899
AclI AA'CG_TT
Cuts at: 1563 4026 4399 1563
Size: 2463 373 3063
Fragments arranged by size:
3063 2463 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2036 3962 5346 5614 1632
Size: 404 1926 1384 268 1917
Fragments arranged by size:
1926 1917 1384 404 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5702
AflIII A'CryG_T
Cuts at: 1203 2019 4798 5775 1203
Size: 816 2779 977 1327
Fragments arranged by size:
2779 1327 977 816
AgeI A'CCGG_T
Cuts at: 4730 4730
Size: 5899
AhdI GACnn_n'nnGTC
Cuts at: 2359 4628 2359
Size: 2269 3630
AleI CACnn'nnGTG
Cuts at: 1166 2260 1166
Size: 1094 4805
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5899
AlwNI CAG_nnn'CTG
Cuts at: 2075 2085 3642 5214 2075
Size: 10 1557 1572 2760
Fragments arranged by size:
2760 1572 1557 10
ApaI G_GGCC'C
Cuts at: 2269 2269
Size: 5899
ApaLI G'TGCA_C
Cuts at: 3956 5112 3956
Size: 1156 4743
ApoI r'AATT_y
Cuts at: 1493 2424 2474 2549 3208 3434 3586 5695 1493
Size: 931 50 75 659 226 152 2109 1697
Fragments arranged by size:
2109 1697 931 659 226 152 75 50
AseI AT'TA_AT
Cuts at: 160 1032 4453 160
Size: 872 3421 1606
Fragments arranged by size:
3421 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
AvaI C'yCGr_G
Cuts at: 1870 1943 2860 3224 1870
Size: 73 917 364 4545
Fragments arranged by size:
4545 917 364 73
BamHI G'GATC_C
Cuts at: 3229 3596 3229
Size: 367 5532
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2151 2161 2315 3220 4675
Size: 325 217 627 364 10 154 905 1455
Cuts at: 4675 618
Size: 1842
Fragments arranged by size:
1842 1455 905 627 364 325 217 154
10
BanII G_rGCy'C
Cuts at: 1391 2269 2373 3218 1391
Size: 878 104 845 4072
Fragments arranged by size:
4072 878 845 104
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4928
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4090 4124 1903
Size: 34 2153 34 3678
Fragments arranged by size:
3678 2153 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3791 5001 3791
Size: 1210 4689
BclI T'GATC_A
Cuts at: 1548 2718 1548
Size: 1170 4729
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4510 136
Size: 107 122 71 631 605 2838 1525
Fragments arranged by size:
2838 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5894 5894
Size: 5899
BlpI GC'TnA_GC
Cuts at: 3316 3316
Size: 5899
Bme1580I G_kGCm'C
Cuts at: 1790 2156 2166 2269 3960 5116 1790
Size: 366 10 103 1691 1156 2573
Fragments arranged by size:
2573 1691 1156 366 103 10
BmgBI CAC'GTC
Cuts at: 1318 2497 1318
Size: 1179 4720
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3630 4588 442
Size: 416 53 83 399 24 2213 958 1753
Fragments arranged by size:
2213 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5899
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4559 1239
Size: 3320 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2717 4024 4889 5187 2717
Size: 1307 865 298 3429
Fragments arranged by size:
3429 1307 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2699 4562 882
Size: 617 1200 1863 2219
Fragments arranged by size:
2219 1863 1200 617
BsaAI yAC'GTr
Cuts at: 493 1204 2020 3690 493
Size: 711 816 1670 2702
Fragments arranged by size:
2702 1670 816 711
BsaBI GATnn'nnATC
Cuts at: 5893 5893
Size: 5899
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3706 4088 4723 275
Size: 53 83 186 936 2173 382 635 1451
Fragments arranged by size:
2173 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4332 4730 5004 5151 5562 4332
Size: 398 274 147 411 4669
Fragments arranged by size:
4669 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2856 2886 1178
Size: 30 165 30 1453 30 4191
Fragments arranged by size:
4191 1453 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3028 1186
Size: 1842 4057
BseYI C'CCAG_C
Cuts at: 5102 5598 5102
Size: 496 5403
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2311 1163
Size: 573 575 4751
Fragments arranged by size:
4751 575 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3349 4110 5138 1540
Size: 275 186 1348 761 1028 2301
Fragments arranged by size:
2301 1348 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2281 2373 3960 4045 5116 2281
Size: 92 1587 85 1071 3064
Fragments arranged by size:
3064 1587 1071 92 85
BsmI GAATG_Cn'
Cuts at: 3410 3503 3410
Size: 93 5806
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2156 2166 2269 2281 2373 3218 3960
Size: 399 366 10 103 12 92 845 742
Cuts at: 3960 4045 5116 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 845 742 399 366 103 92
85 12 10
BspEI T'CCGG_A
Cuts at: 5562 5562
Size: 5899
BspHI T'CATG_A
Cuts at: 3788 5518 3788
Size: 1730 4169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3254 844
Size: 2410 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3786 1394
Size: 429 1963 3507
Fragments arranged by size:
3507 1963 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4394 4568 65
Size: 1183 3146 174 1396
Fragments arranged by size:
3146 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2088 2795 4543 4730 1700
Size: 110 278 707 1748 187 2869
Fragments arranged by size:
2869 1748 707 278 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5899
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5899
BssSI C'ACGA_G
Cuts at: 3959 4971 3959
Size: 1012 4887
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5899
BstBI TT'CG_AA
Cuts at: 4703 4703
Size: 5899
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5899
BstYI r'GATC_y
Cuts at: 1972 3229 3596 3981 3998 5450 5536 5548 5894
Size: 1257 367 385 17 1452 86 12 346
Cuts at: 5894 1972
Size: 1977
Fragments arranged by size:
1977 1452 1257 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2147 2147
Size: 5899
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5347
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5160
Fragments arranged by size:
5160 683 56
BtsI GCAGTG_nn'
Cuts at: 3415 4209 4229 3415
Size: 794 20 5085
Fragments arranged by size:
5085 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5899
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5864
DraI TTT'AAA
Cuts at: 2977 3203 2977
Size: 226 5673
DraIII CAC_nnn'GTG
Cuts at: 2593 2593
Size: 5899
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4906 809
Size: 507 3590 1802
Fragments arranged by size:
3590 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2829 3346 4235
Size: 54 1425 50 275 18 999 517 889
Cuts at: 4235 5864 8
Size: 1629 43
Fragments arranged by size:
1629 1425 999 889 517 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3346 1537
Size: 275 1534 4090
Fragments arranged by size:
4090 1534 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2244 2529 2697 3829 1353
Size: 891 285 168 1132 3423
Fragments arranged by size:
3423 1132 891 285 168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4486 4860 5006 387
Size: 1064 138 2897 374 146 1280
Fragments arranged by size:
2897 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2371 2371
Size: 5899
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 3962 4559 5346 5614 1239
Size: 393 404 1926 597 787 268 1524
Fragments arranged by size:
1926 1524 787 597 404 393 268
EcoRI G'AATT_C
Cuts at: 2474 3208 3586 2474
Size: 734 378 4787
Fragments arranged by size:
4787 734 378
FspI TGC'GCA
Cuts at: 4405 4405
Size: 5899
HaeII r_GCGC'y
Cuts at: 1678 2856 5046 1678
Size: 1178 2190 2531
Fragments arranged by size:
2531 2190 1178
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2345 2377 2754 2786 2926
Size: 32 135 32 773 32 377 32 140
Cuts at: 2926 2958 3170 3202 4540 4572 4614 4646 1373
Size: 32 212 32 1338 32 42 32 2626
Fragments arranged by size:
2626 1338 773 377 212 140 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2439 3243 3489 669
Size: 988 281 501 804 246 3079
Fragments arranged by size:
3079 988 804 501 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5899
HpaI GTT'AAC
Cuts at: 3489 3489
Size: 5899
KpnI G_GTAC'C
Cuts at: 3224 3224
Size: 5899
MfeI C'AATT_G
Cuts at: 3498 3498
Size: 5899
MluI A'CGCG_T
Cuts at: 5775 5775
Size: 5899
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5013 5197 1305
Size: 3708 184 2007
Fragments arranged by size:
3708 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4077
Fragments arranged by size:
4077 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2217 2260 2714 2924 3857 4216 4375
Size: 648 1051 43 454 210 933 359 159
Cuts at: 4375 518
Size: 2042
Fragments arranged by size:
2042 1051 933 648 454 359 210 159
43
MspA1I CmG'CkG
Cuts at: 1606 2622 3315 3992 5140 5385 1606
Size: 1016 693 677 1148 245 2120
Fragments arranged by size:
2120 1148 1016 693 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5347
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5899
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5899
NotI GC'GGCC_GC
Cuts at: 3346 3346
Size: 5899
NspI r_CATG'y
Cuts at: 1132 1229 3257 4802 1132
Size: 97 2028 1545 2229
Fragments arranged by size:
2229 2028 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5899
PciI A'CATG_T
Cuts at: 4798 4798
Size: 5899
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2820 1108
Size: 1712 4187
PmeI GTTT'AAAC
Cuts at: 3203 3203
Size: 5899
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3968 4000 5481 5513 3968
Size: 32 1481 32 4354
Fragments arranged by size:
4354 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5899
PsiI TTA'TAA
Cuts at: 3469 3469
Size: 5899
PspOMI G'GGCC_C
Cuts at: 2265 2265
Size: 5899
PstI C_TGCA'G
Cuts at: 830 3251 830
Size: 2421 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5899
SacI G_AGCT'C
Cuts at: 2373 2373
Size: 5899
SalI G'TCGA_C
Cuts at: 1936 3241 1936
Size: 1305 4594
SbfI CC_TGCA'GG
Cuts at: 3251 3251
Size: 5899
ScaI AGT'ACT
Cuts at: 4147 5859 4147
Size: 1712 4187
SfcI C'TryA_G
Cuts at: 826 1045 2139 3247 4382 5063 5254 826
Size: 219 1094 1108 1135 681 191 1471
Fragments arranged by size:
1471 1135 1108 1094 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5899
SmaI CCC'GGG
Cuts at: 1872 2862 3226 1872
Size: 990 364 4545
Fragments arranged by size:
4545 990 364
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2732 4003 4904 5166 820
Size: 197 926 789 1271 901 262 1553
Fragments arranged by size:
1553 1271 926 901 789 262 197
SnaBI TAC'GTA
Cuts at: 493 3690 493
Size: 3197 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5899
SphI G_CATG'C
Cuts at: 1132 3257 1132
Size: 2125 3774
SspI AAT'ATT
Cuts at: 5 52 2897 3823 5
Size: 47 2845 926 2081
Fragments arranged by size:
2845 2081 926 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5899
StyI C'CwwG_G
Cuts at: 513 1065 3195 3338 513
Size: 552 2130 143 3074
Fragments arranged by size:
3074 2130 552 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2855 2891 3926 3943 4096 4281 4726
Size: 1255 1275 36 1035 17 153 185 445
Cuts at: 4726 325
Size: 1498
Fragments arranged by size:
1498 1275 1255 1035 445 185 153 36
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4145 5857 96
Size: 274 80 33 51 1443 2168 1712 138
Fragments arranged by size:
2168 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2856 2888 5859 5891 2856
Size: 32 2971 32 2864
Fragments arranged by size:
2971 2864 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5899
XbaI T'CTAG_A
Cuts at: 3235 3235
Size: 5899
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5899
XmaI C'CCGG_G
Cuts at: 1870 2860 3224 1870
Size: 990 364 4545
Fragments arranged by size:
4545 990 364
XmnI GAAnn'nnTTC
Cuts at: 2341 4028 2341
Size: 1687 4212
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3707 276
Size: 53 83 186 936 2173 2468
Fragments arranged by size:
2468 2173 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI