Restriction map for
pFN21AE1876
(Circular) (Six-base) MAPSORT of: pFN21AE1876.seq Check: 4197 from: 1 to: 5899
pFN21AE1876 5899 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2263 2263
Size: 5899
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3709 278
Size: 53 83 186 936 2173 2468
Fragments arranged by size:
2468 2173 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3242 1937
Size: 1305 4594
Acc65I G'GTAC_C
Cuts at: 3220 3220
Size: 5899
AclI AA'CG_TT
Cuts at: 1563 4026 4399 1563
Size: 2463 373 3063
Fragments arranged by size:
3063 2463 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2588 3962 5346 5614 1632
Size: 956 1374 1384 268 1917
Fragments arranged by size:
1917 1384 1374 956 268
AfeI AGC'GCT
Cuts at: 2534 2594 2534
Size: 60 5839
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5702
AflIII A'CryG_T
Cuts at: 1203 4798 5775 1203
Size: 3595 977 1327
Fragments arranged by size:
3595 1327 977
AgeI A'CCGG_T
Cuts at: 4730 4730
Size: 5899
AhdI GACnn_n'nnGTC
Cuts at: 4628 4628
Size: 5899
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5899
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5899
AlwNI CAG_nnn'CTG
Cuts at: 3642 5214 3642
Size: 1572 4327
ApaLI G'TGCA_C
Cuts at: 3956 5112 3956
Size: 1156 4743
ApoI r'AATT_y
Cuts at: 1493 2572 3059 3208 3434 3586 5695 1493
Size: 1079 487 149 226 152 2109 1697
Fragments arranged by size:
2109 1697 1079 487 226 152 149
AseI AT'TA_AT
Cuts at: 160 1032 4453 160
Size: 872 3421 1606
Fragments arranged by size:
3421 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
AvaI C'yCGr_G
Cuts at: 1870 1943 2855 3224 1870
Size: 73 912 369 4545
Fragments arranged by size:
4545 912 369 73
BamHI G'GATC_C
Cuts at: 2040 3016 3229 3596 2040
Size: 976 213 367 4343
Fragments arranged by size:
4343 976 367 213
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2915 3220 4675 618
Size: 325 217 627 1128 305 1455 1842
Fragments arranged by size:
1842 1455 1128 627 325 305 217
BanII G_rGCy'C
Cuts at: 1391 3218 1391
Size: 1827 4072
BbeI G_GCGC'C
Cuts at: 2919 2919
Size: 5899
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2285 928
Size: 971 386 4542
Fragments arranged by size:
4542 971 386
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4090 4124 1903
Size: 34 2153 34 3678
Fragments arranged by size:
3678 2153 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3791 5001 3791
Size: 1210 4689
BclI T'GATC_A
Cuts at: 1548 2539 1548
Size: 991 4908
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4510 136
Size: 107 122 71 631 605 2838 1525
Fragments arranged by size:
2838 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5894 5894
Size: 5899
BlpI GC'TnA_GC
Cuts at: 3316 3316
Size: 5899
Bme1580I G_kGCm'C
Cuts at: 1790 3960 5116 1790
Size: 2170 1156 2573
Fragments arranged by size:
2573 2170 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5899
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2952 3630 4588
Size: 416 53 83 399 24 1535 678 958
Cuts at: 4588 442
Size: 1753
Fragments arranged by size:
1753 1535 958 678 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5899
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3197 4559 1239
Size: 1958 1362 2579
Fragments arranged by size:
2579 1958 1362
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2902 4024 4889 5187 2902
Size: 1122 865 298 3614
Fragments arranged by size:
3614 1122 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2952 4562 882
Size: 617 1453 1610 2219
Fragments arranged by size:
2219 1610 1453 617
BsaAI yAC'GTr
Cuts at: 493 1204 3690 493
Size: 711 2486 2702
Fragments arranged by size:
2702 2486 711
BsaBI GATnn'nnATC
Cuts at: 2478 5893 2478
Size: 3415 2484
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2916 3706 4088 4723
Size: 53 83 186 936 1383 790 382 635
Cuts at: 4723 275
Size: 1451
Fragments arranged by size:
1451 1383 936 790 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4332 4730 5004 5151 5562 4332
Size: 398 274 147 411 4669
Fragments arranged by size:
4669 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2198 2228 2664 2694 1178
Size: 30 165 30 795 30 436 30 4383
Fragments arranged by size:
4383 795 436 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2247 1186
Size: 1061 4838
BseYI C'CCAG_C
Cuts at: 2989 5102 5598 2989
Size: 2113 496 3290
Fragments arranged by size:
3290 2113 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5326
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3349 4110 5138 1540
Size: 275 186 1348 761 1028 2301
Fragments arranged by size:
2301 1348 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3960 4045 5116 3960
Size: 85 1071 4743
Fragments arranged by size:
4743 1071 85
BsmI GAATG_Cn'
Cuts at: 3410 3503 3410
Size: 93 5806
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3218 3960 4045 5116 1391
Size: 399 1428 742 85 1071 2174
Fragments arranged by size:
2174 1428 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5562 5562
Size: 5899
BspHI T'CATG_A
Cuts at: 3788 5518 3788
Size: 1730 4169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2263 3254 844
Size: 1419 991 3489
Fragments arranged by size:
3489 1419 991
BsrBI CCG'CTC
Cuts at: 1394 1823 3181 3786 1394
Size: 429 1358 605 3507
Fragments arranged by size:
3507 1358 605 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2097 4394 4568 65
Size: 1183 849 2297 174 1396
Fragments arranged by size:
2297 1396 1183 849 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4543 4730 1700
Size: 110 2733 187 2869
Fragments arranged by size:
2869 2733 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5899
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5899
BssSI C'ACGA_G
Cuts at: 2192 3959 4971 2192
Size: 1767 1012 3120
Fragments arranged by size:
3120 1767 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5899
BstBI TT'CG_AA
Cuts at: 4703 4703
Size: 5899
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5899
BstYI r'GATC_y
Cuts at: 1972 2040 2503 3016 3229 3596 3981 3998 5450
Size: 68 463 513 213 367 385 17 1452
Cuts at: 5450 5536 5548 5894 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 513 463 385 367 346 213
86 68 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2245 2787 513
Size: 552 1180 542 3625
Fragments arranged by size:
3625 1180 552 542
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5160
Fragments arranged by size:
5160 683 56
BtsI GCAGTG_nn'
Cuts at: 3415 4209 4229 3415
Size: 794 20 5085
Fragments arranged by size:
5085 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5899
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5864
DraI TTT'AAA
Cuts at: 3203 3203
Size: 5899
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4906 809
Size: 507 3590 1802
Fragments arranged by size:
3590 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2172 2784 3346
Size: 54 1425 50 275 18 342 612 562
Cuts at: 3346 4235 5864 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 612 562 342 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3346 1537
Size: 275 1534 4090
Fragments arranged by size:
4090 1534 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3829 1353
Size: 2476 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4486 4860 5006 387
Size: 1064 138 2897 374 146 1280
Fragments arranged by size:
2897 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2588 3197 3962 4559 5346 5614 1239
Size: 393 956 609 765 597 787 268 1524
Fragments arranged by size:
1524 956 787 765 609 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2079 2079
Size: 5899
EcoRI G'AATT_C
Cuts at: 3208 3586 3208
Size: 378 5521
EcoRV GAT'ATC
Cuts at: 2375 2375
Size: 5899
FspI TGC'GCA
Cuts at: 4405 4405
Size: 5899
HaeII r_GCGC'y
Cuts at: 1678 2536 2596 2919 5046 1678
Size: 858 60 323 2127 2531
Fragments arranged by size:
2531 2127 858 323 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2071 2103 2932 2964 4540
Size: 32 135 32 499 32 829 32 1576
Cuts at: 4540 4572 4614 4646 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1576 829 499 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3243 3489 669
Size: 988 281 1305 246 3079
Fragments arranged by size:
3079 1305 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2292 748
Size: 1544 4355
HpaI GTT'AAC
Cuts at: 3489 3489
Size: 5899
KasI G'GCGC_C
Cuts at: 2915 2915
Size: 5899
KpnI G_GTAC'C
Cuts at: 3224 3224
Size: 5899
MfeI C'AATT_G
Cuts at: 3094 3498 3094
Size: 404 5495
MluI A'CGCG_T
Cuts at: 5775 5775
Size: 5899
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2031 2704 3066 5013 5197 1305
Size: 726 673 362 1947 184 2007
Fragments arranged by size:
2007 1947 726 673 362 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4077
Fragments arranged by size:
4077 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2436 3857 4216 4375 518
Size: 648 1270 1421 359 159 2042
Fragments arranged by size:
2042 1421 1270 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3315 3992 5140 5385 1606
Size: 1709 677 1148 245 2120
Fragments arranged by size:
2120 1709 1148 677 245
NarI GG'CG_CC
Cuts at: 2916 2916
Size: 5899
NcoI C'CATG_G
Cuts at: 513 1065 2245 513
Size: 552 1180 4167
Fragments arranged by size:
4167 1180 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5899
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5899
NotI GC'GGCC_GC
Cuts at: 3346 3346
Size: 5899
NspI r_CATG'y
Cuts at: 1132 1229 3257 4802 1132
Size: 97 2028 1545 2229
Fragments arranged by size:
2229 2028 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2057 1348
Size: 709 5190
PciI A'CATG_T
Cuts at: 4798 4798
Size: 5899
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2050 2227 1108
Size: 942 177 4780
Fragments arranged by size:
4780 942 177
PmeI GTTT'AAAC
Cuts at: 3203 3203
Size: 5899
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2740 2772 3131 3163 3968 4000 5481 5513 2740
Size: 32 359 32 805 32 1481 32 3126
Fragments arranged by size:
3126 1481 805 359 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2079 2079
Size: 5899
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5899
PsiI TTA'TAA
Cuts at: 3469 3469
Size: 5899
PstI C_TGCA'G
Cuts at: 830 2514 3251 830
Size: 1684 737 3478
Fragments arranged by size:
3478 1684 737
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5899
SalI G'TCGA_C
Cuts at: 1936 3241 1936
Size: 1305 4594
SanDI GG'GwC_CC
Cuts at: 2079 2079
Size: 5899
SbfI CC_TGCA'GG
Cuts at: 3251 3251
Size: 5899
ScaI AGT'ACT
Cuts at: 4147 5859 4147
Size: 1712 4187
SexAI A'CCwGG_T
Cuts at: 2118 2118
Size: 5899
SfcI C'TryA_G
Cuts at: 826 1045 2510 3120 3247 4382 5063 5254 826
Size: 219 1465 610 127 1135 681 191 1471
Fragments arranged by size:
1471 1465 1135 681 610 219 191 127
SfoI GGC'GCC
Cuts at: 2917 2917
Size: 5899
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5899
SmaI CCC'GGG
Cuts at: 1872 3226 1872
Size: 1354 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2881 4003 4904 5166 820
Size: 197 926 938 1122 901 262 1553
Fragments arranged by size:
1553 1122 938 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3690 493
Size: 3197 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5899
SphI G_CATG'C
Cuts at: 1132 3257 1132
Size: 2125 3774
SspI AAT'ATT
Cuts at: 5 52 3823 5
Size: 47 3771 2081
Fragments arranged by size:
3771 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5899
StyI C'CwwG_G
Cuts at: 513 1065 2245 3338 513
Size: 552 1180 1093 3074
Fragments arranged by size:
3074 1180 1093 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2476 3926 3943 4096 4281 4726 325
Size: 1255 896 1450 17 153 185 445 1498
Fragments arranged by size:
1498 1450 1255 896 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4145 5857 96
Size: 274 80 33 51 1443 2168 1712 138
Fragments arranged by size:
2168 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5859 5891 5859
Size: 32 5867
Tth111I GACn'n_nGTC
Cuts at: 1316 2944 1316
Size: 1628 4271
XbaI T'CTAG_A
Cuts at: 3235 3235
Size: 5899
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5899
XmaI C'CCGG_G
Cuts at: 1870 3224 1870
Size: 1354 4545
XmnI GAAnn'nnTTC
Cuts at: 2282 2573 4028 2282
Size: 291 1455 4153
Fragments arranged by size:
4153 1455 291
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3707 276
Size: 53 83 186 936 2173 2468
Fragments arranged by size:
2468 2173 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SanDI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SapI SfiI SrfI SwaI XcmI