Restriction map for
pFN21AE1875
(Circular) (Six-base) MAPSORT of: pFN21AE1875.seq Check: 392 from: 1 to: 5899
pFN21AE1875 5899 bp
With 182 enzymes: SgfI *
July 27, 2017 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2738 2738
Size: 5899
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3709 278
Size: 53 83 186 936 2173 2468
Fragments arranged by size:
2468 2173 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3164 3242 1937
Size: 1227 78 4594
Fragments arranged by size:
4594 1227 78
Acc65I G'GTAC_C
Cuts at: 3220 3220
Size: 5899
AclI AA'CG_TT
Cuts at: 1563 4026 4399 1563
Size: 2463 373 3063
Fragments arranged by size:
3063 2463 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3962 5346 5614 1632
Size: 2330 1384 268 1917
Fragments arranged by size:
2330 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5702
AflIII A'CryG_T
Cuts at: 1203 4798 5775 1203
Size: 3595 977 1327
Fragments arranged by size:
3595 1327 977
AgeI A'CCGG_T
Cuts at: 4730 4730
Size: 5899
AhdI GACnn_n'nnGTC
Cuts at: 4628 4628
Size: 5899
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5899
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5899
AlwNI CAG_nnn'CTG
Cuts at: 2517 2623 3139 3642 5214 2517
Size: 106 516 503 1572 3202
Fragments arranged by size:
3202 1572 516 503 106
ApaI G_GGCC'C
Cuts at: 2251 2423 2251
Size: 172 5727
ApaLI G'TGCA_C
Cuts at: 3956 5112 3956
Size: 1156 4743
ApoI r'AATT_y
Cuts at: 1493 3208 3434 3586 5695 1493
Size: 1715 226 152 2109 1697
Fragments arranged by size:
2109 1715 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4453 160
Size: 872 3421 1606
Fragments arranged by size:
3421 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
AvaI C'yCGr_G
Cuts at: 1870 1943 2290 3224 1870
Size: 73 347 934 4545
Fragments arranged by size:
4545 934 347 73
BamHI G'GATC_C
Cuts at: 3229 3596 3229
Size: 367 5532
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2270 3220 4675 618
Size: 325 217 627 483 950 1455 1842
Fragments arranged by size:
1842 1455 950 627 483 325 217
BanII G_rGCy'C
Cuts at: 1391 2235 2251 2423 2463 3218 1391
Size: 844 16 172 40 755 4072
Fragments arranged by size:
4072 844 755 172 40 16
BbeI G_GCGC'C
Cuts at: 2274 2274
Size: 5899
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4928
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4090 4124 1903
Size: 34 2153 34 3678
Fragments arranged by size:
3678 2153 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3791 5001 3791
Size: 1210 4689
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5899
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2295 4510 136
Size: 107 122 71 631 605 623 2215 1525
Fragments arranged by size:
2215 1525 631 623 605 122 107 71
BglII A'GATC_T
Cuts at: 5894 5894
Size: 5899
BlpI GC'TnA_GC
Cuts at: 2679 3316 2679
Size: 637 5262
Bme1580I G_kGCm'C
Cuts at: 1790 2251 2364 2423 3960 5116 1790
Size: 461 113 59 1537 1156 2573
Fragments arranged by size:
2573 1537 1156 461 113 59
BmgBI CAC'GTC
Cuts at: 1318 2749 1318
Size: 1431 4468
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2303 2351 2597
Size: 416 53 83 399 24 886 48 246
Cuts at: 2597 3630 4588 442
Size: 1033 958 1753
Fragments arranged by size:
1753 1033 958 886 416 399 246 83
53 48 24
BmtI G_CTAG'C
Cuts at: 1056 2340 1056
Size: 1284 4615
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2287 2477 4559 1239
Size: 1048 190 2082 2579
Fragments arranged by size:
2579 2082 1048 190
Bpu10I CC'TnA_GC
Cuts at: 2027 2144 2027
Size: 117 5782
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2561 3019 4024 4889 5187 2561
Size: 458 1005 865 298 3273
Fragments arranged by size:
3273 1005 865 458 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4562 882
Size: 617 3063 2219
Fragments arranged by size:
3063 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3690 493
Size: 711 2486 2702
Fragments arranged by size:
2702 2486 711
BsaBI GATnn'nnATC
Cuts at: 5893 5893
Size: 5899
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2109 2271 3706 4088
Size: 53 83 186 936 576 162 1435 382
Cuts at: 4088 4723 275
Size: 635 1451
Fragments arranged by size:
1451 1435 936 635 576 382 186 162
83 53
BsaWI w'CCGG_w
Cuts at: 2235 2240 4332 4730 5004 5151 5562 2235
Size: 5 2092 398 274 147 411 2572
Fragments arranged by size:
2572 2092 411 398 274 147 5
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5674
Fragments arranged by size:
5674 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2253 2266 2414 2517 2675 2952 1186
Size: 1067 13 148 103 158 277 4133
Fragments arranged by size:
4133 1067 277 158 148 103 13
BseYI C'CCAG_C
Cuts at: 2280 2931 5102 5598 2280
Size: 651 2171 496 2581
Fragments arranged by size:
2581 2171 651 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2084 1163
Size: 573 348 4978
Fragments arranged by size:
4978 573 348
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2255 2500 3349 4110 5138 1540
Size: 275 186 254 245 849 761 1028 2301
Fragments arranged by size:
2301 1028 849 761 275 254 245 186
BsiHKAI G_wGCw'C
Cuts at: 2235 2463 2574 3960 4045 5116 2235
Size: 228 111 1386 85 1071 3018
Fragments arranged by size:
3018 1386 1071 228 111 85
BsmI GAATG_Cn'
Cuts at: 3410 3503 3410
Size: 93 5806
BsmBI CGTCTCn'nnnn_
Cuts at: 2527 2527
Size: 5899
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2235 2251 2364 2423 2463 2574 3218
Size: 399 445 16 113 59 40 111 644
Cuts at: 3218 3960 4045 5116 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 644 445 399 113 111
85 59 40 16
BspEI T'CCGG_A
Cuts at: 2235 5562 2235
Size: 3327 2572
BspHI T'CATG_A
Cuts at: 3788 5518 3788
Size: 1730 4169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2738 3254 844
Size: 1894 516 3489
Fragments arranged by size:
3489 1894 516
BsrBI CCG'CTC
Cuts at: 1394 1823 2023 2031 3786 1394
Size: 429 200 8 1755 3507
Fragments arranged by size:
3507 1755 429 200 8
BsrDI GCAATG_nn'
Cuts at: 65 1248 2826 4394 4568 65
Size: 1183 1578 1568 174 1396
Fragments arranged by size:
1578 1568 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2562 4543 4730 1700
Size: 110 752 1981 187 2869
Fragments arranged by size:
2869 1981 752 187 110
BsrGI T'GTAC_A
Cuts at: 96 3145 96
Size: 3049 2850
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5899
BssSI C'ACGA_G
Cuts at: 2081 2462 3959 4971 2081
Size: 381 1497 1012 3009
Fragments arranged by size:
3009 1497 1012 381
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5899
BstBI TT'CG_AA
Cuts at: 4703 4703
Size: 5899
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5899
BstYI r'GATC_y
Cuts at: 1972 3229 3596 3981 3998 5450 5536 5548 5894
Size: 1257 367 385 17 1452 86 12 346
Cuts at: 5894 1972
Size: 1977
Fragments arranged by size:
1977 1452 1257 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3165 3165
Size: 5899
Bsu36I CC'TnA_GG
Cuts at: 2397 2397
Size: 5899
BtgI C'CryG_G
Cuts at: 513 1065 2801 513
Size: 552 1736 3611
Fragments arranged by size:
3611 1736 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2057 487
Size: 683 56 831 4329
Fragments arranged by size:
4329 831 683 56
BtsI GCAGTG_nn'
Cuts at: 3415 4209 4229 3415
Size: 794 20 5085
Fragments arranged by size:
5085 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5899
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2985 3020 529
Size: 35 2421 35 3408
Fragments arranged by size:
3408 2421 35 35
DraI TTT'AAA
Cuts at: 3203 3203
Size: 5899
DraIII CAC_nnn'GTG
Cuts at: 2536 2536
Size: 5899
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4906 809
Size: 507 3590 1802
Fragments arranged by size:
3590 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2085 2134 2252
Size: 54 1425 50 275 18 255 49 118
Cuts at: 2252 2497 2538 3346 4235 5864 8
Size: 245 41 808 889 1629 43
Fragments arranged by size:
1629 1425 889 808 275 255 245 118
54 50 49 43 41 18
EagI C'GGCC_G
Cuts at: 1537 1812 2252 2497 3346 1537
Size: 275 440 245 849 4090
Fragments arranged by size:
4090 849 440 275 245
EarI CTCTTCn'nnn_
Cuts at: 1353 3829 1353
Size: 2476 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3017 4486 4860 5006 387
Size: 1064 138 1428 1469 374 146 1280
Fragments arranged by size:
1469 1428 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2233 2461 2233
Size: 228 5671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2287 2477 3962 4559 5346 5614 1239
Size: 393 655 190 1485 597 787 268 1524
Fragments arranged by size:
1524 1485 787 655 597 393 268 190
EcoNI CCTnn'n_nnAGG
Cuts at: 2265 2265
Size: 5899
EcoO109I rG'GnC_Cy
Cuts at: 2247 2420 2247
Size: 173 5726
EcoRI G'AATT_C
Cuts at: 3208 3586 3208
Size: 378 5521
FspI TGC'GCA
Cuts at: 4405 4405
Size: 5899
HaeII r_GCGC'y
Cuts at: 1678 2040 2274 2617 5046 1678
Size: 362 234 343 2429 2531
Fragments arranged by size:
2531 2429 362 343 234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2507 2539 4540 4572 4614
Size: 32 135 32 935 32 2001 32 42
Cuts at: 4614 4646 1373
Size: 32 2626
Fragments arranged by size:
2626 2001 935 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3243 3489 669
Size: 988 281 1305 246 3079
Fragments arranged by size:
3079 1305 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5899
HpaI GTT'AAC
Cuts at: 3489 3489
Size: 5899
KasI G'GCGC_C
Cuts at: 2270 2270
Size: 5899
KpnI G_GTAC'C
Cuts at: 3224 3224
Size: 5899
MfeI C'AATT_G
Cuts at: 3498 3498
Size: 5899
MluI A'CGCG_T
Cuts at: 5775 5775
Size: 5899
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2146 5013 5197 1305
Size: 841 2867 184 2007
Fragments arranged by size:
2867 2007 841 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2540 10
Size: 54 1425 343 708 3369
Fragments arranged by size:
3369 1425 708 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2447 2762 3857 4216 4375 518
Size: 648 1281 315 1095 359 159 2042
Fragments arranged by size:
2042 1281 1095 648 359 315 159
MspA1I CmG'CkG
Cuts at: 1606 2065 2182 2410 2623 3315 3992 5140 5385
Size: 459 117 228 213 692 677 1148 245
Cuts at: 5385 1606
Size: 2120
Fragments arranged by size:
2120 1148 692 677 459 245 228 213
117
NarI GG'CG_CC
Cuts at: 2271 2271
Size: 5899
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5347
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5899
NheI G'CTAG_C
Cuts at: 1052 2336 1052
Size: 1284 4615
NotI GC'GGCC_GC
Cuts at: 2497 3346 2497
Size: 849 5050
NspI r_CATG'y
Cuts at: 1132 1229 2446 2983 3257 4802 1132
Size: 97 1217 537 274 1545 2229
Fragments arranged by size:
2229 1545 1217 537 274 97
PasI CC'CwG_GG
Cuts at: 1348 2511 1348
Size: 1163 4736
PciI A'CATG_T
Cuts at: 4798 4798
Size: 5899
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2623 3139 1108
Size: 1515 516 3868
Fragments arranged by size:
3868 1515 516
PmeI GTTT'AAAC
Cuts at: 3203 3203
Size: 5899
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3968 4000 5481 5513 3968
Size: 32 1481 32 4354
Fragments arranged by size:
4354 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2351 1613
Size: 738 5161
PsiI TTA'TAA
Cuts at: 3469 3469
Size: 5899
PspOMI G'GGCC_C
Cuts at: 2247 2419 2247
Size: 172 5727
PstI C_TGCA'G
Cuts at: 830 2187 2613 3251 830
Size: 1357 426 638 3478
Fragments arranged by size:
3478 1357 638 426
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5899
PvuII CAG'CTG
Cuts at: 2623 2623
Size: 5899
RsrII CG'GwC_CG
Cuts at: 2238 2238
Size: 5899
SacI G_AGCT'C
Cuts at: 2235 2463 2235
Size: 228 5671
SalI G'TCGA_C
Cuts at: 1936 3241 1936
Size: 1305 4594
SbfI CC_TGCA'GG
Cuts at: 3251 3251
Size: 5899
ScaI AGT'ACT
Cuts at: 2582 4147 5859 2582
Size: 1565 1712 2622
Fragments arranged by size:
2622 1712 1565
SexAI A'CCwGG_T
Cuts at: 3132 3132
Size: 5899
SfcI C'TryA_G
Cuts at: 826 1045 2060 2183 2609 3247 4382 5063 5254
Size: 219 1015 123 426 638 1135 681 191
Cuts at: 5254 826
Size: 1471
Fragments arranged by size:
1471 1135 1015 681 638 426 219 191
123
SfoI GGC'GCC
Cuts at: 2272 2272
Size: 5899
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5899
SmaI CCC'GGG
Cuts at: 1872 2292 3226 1872
Size: 420 934 4545
Fragments arranged by size:
4545 934 420
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2576 3034 4003 4904 5166 820
Size: 197 926 633 458 969 901 262 1553
Fragments arranged by size:
1553 969 926 901 633 458 262 197
SnaBI TAC'GTA
Cuts at: 493 3690 493
Size: 3197 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5899
SphI G_CATG'C
Cuts at: 1132 3257 1132
Size: 2125 3774
SspI AAT'ATT
Cuts at: 5 52 3823 5
Size: 47 3771 2081
Fragments arranged by size:
3771 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5899
StyI C'CwwG_G
Cuts at: 513 1065 2104 3338 513
Size: 552 1039 1234 3074
Fragments arranged by size:
3074 1234 1039 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2341 2616 3090 3926 3943 4096 4281
Size: 1255 761 275 474 836 17 153 185
Cuts at: 4281 4726 325
Size: 445 1498
Fragments arranged by size:
1498 1255 836 761 474 445 275 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2580 3145 4145
Size: 274 80 33 51 1443 603 565 1000
Cuts at: 4145 5857 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1000 603 565 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5859 5891 5859
Size: 32 5867
Tth111I GACn'n_nGTC
Cuts at: 1316 2519 1316
Size: 1203 4696
XbaI T'CTAG_A
Cuts at: 3235 3235
Size: 5899
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2311 2311
Size: 5899
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5899
XmaI C'CCGG_G
Cuts at: 1870 2290 3224 1870
Size: 420 934 4545
Fragments arranged by size:
4545 934 420
XmnI GAAnn'nnTTC
Cuts at: 4028 4028
Size: 5899
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3707 276
Size: 53 83 186 936 2173 2468
Fragments arranged by size:
2468 2173 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BstXI EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
SacII SanDI SapI SfiI SrfI SwaI