Restriction map for
pFN21AE1874
(Circular) (Six-base) MAPSORT of: pFN21AE1874.seq Check: 1937 from: 1 to: 5893
pFN21AE1874 5893 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3703 278
Size: 53 83 186 936 2167 2468
Fragments arranged by size:
2468 2167 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2526 3236 1937
Size: 589 710 4594
Fragments arranged by size:
4594 710 589
Acc65I G'GTAC_C
Cuts at: 3214 3214
Size: 5893
AclI AA'CG_TT
Cuts at: 1563 4020 4393 1563
Size: 2457 373 3063
Fragments arranged by size:
3063 2457 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3956 5340 5608 1632
Size: 2324 1384 268 1917
Fragments arranged by size:
2324 1917 1384 268
AfeI AGC'GCT
Cuts at: 2222 2222
Size: 5893
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5696
AflIII A'CryG_T
Cuts at: 1203 2352 2553 4792 5769 1203
Size: 1149 201 2239 977 1327
Fragments arranged by size:
2239 1327 1149 977 201
AgeI A'CCGG_T
Cuts at: 2866 4724 2866
Size: 1858 4035
AhdI GACnn_n'nnGTC
Cuts at: 4622 4622
Size: 5893
AleI CACnn'nnGTG
Cuts at: 1166 2717 1166
Size: 1551 4342
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2816 1184
Size: 1632 4261
AlwNI CAG_nnn'CTG
Cuts at: 3636 5208 3636
Size: 1572 4321
ApaLI G'TGCA_C
Cuts at: 3950 5106 3950
Size: 1156 4737
ApoI r'AATT_y
Cuts at: 1493 3202 3428 3580 5689 1493
Size: 1709 226 152 2109 1697
Fragments arranged by size:
2109 1709 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2492 2492
Size: 5893
AseI AT'TA_AT
Cuts at: 160 1032 4447 160
Size: 872 3415 1606
Fragments arranged by size:
3415 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
AvaI C'yCGr_G
Cuts at: 1870 1943 2832 3218 1870
Size: 73 889 386 4545
Fragments arranged by size:
4545 889 386 73
BamHI G'GATC_C
Cuts at: 3223 3590 3223
Size: 367 5526
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2100 2177 2707 2814 2842
Size: 325 217 627 313 77 530 107 28
Cuts at: 2842 2908 3214 4669 618
Size: 66 306 1455 1842
Fragments arranged by size:
1842 1455 627 530 325 313 306 217
107 77 66 28
BanII G_rGCy'C
Cuts at: 1391 2216 3212 1391
Size: 825 996 4072
Fragments arranged by size:
4072 996 825
BbeI G_GCGC'C
Cuts at: 2104 2181 2846 2912 2104
Size: 77 665 66 5085
Fragments arranged by size:
5085 665 77 66
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4922
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2104 2138 2420 2454 3124 3158 4084
Size: 34 167 34 282 34 670 34 926
Cuts at: 4084 4118 1903
Size: 34 3678
Fragments arranged by size:
3678 926 670 282 167 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3785 4995 3785
Size: 1210 4683
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5893
BfrBI ATG'CAT
Cuts at: 3145 3145
Size: 5893
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2951 4504 136
Size: 107 122 71 631 605 1279 1553 1525
Fragments arranged by size:
1553 1525 1279 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5888 5888
Size: 5893
BlpI GC'TnA_GC
Cuts at: 3172 3310 3172
Size: 138 5755
Bme1580I G_kGCm'C
Cuts at: 1790 2154 2241 2712 3954 5110 1790
Size: 364 87 471 1242 1156 2573
Fragments arranged by size:
2573 1242 1156 471 364 87
BmgBI CAC'GTC
Cuts at: 1318 2548 1318
Size: 1230 4663
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2530 2683 3197
Size: 416 53 83 399 24 1113 153 514
Cuts at: 3197 3624 4582 442
Size: 427 958 1753
Fragments arranged by size:
1753 1113 958 514 427 416 399 153
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5893
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3065 4553 1239
Size: 1826 1488 2579
Fragments arranged by size:
2579 1826 1488
Bpu10I CC'TnA_GC
Cuts at: 2412 2412
Size: 5893
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3103 4018 4883 5181 3103
Size: 915 865 298 3815
Fragments arranged by size:
3815 915 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2768 4556 882
Size: 617 1269 1788 2219
Fragments arranged by size:
2219 1788 1269 617
BsaAI yAC'GTr
Cuts at: 493 1204 2554 3684 493
Size: 711 1350 1130 2702
Fragments arranged by size:
2702 1350 1130 711
BsaBI GATnn'nnATC
Cuts at: 5887 5887
Size: 5893
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2101 2178 2843 2909
Size: 53 83 186 936 568 77 665 66
Cuts at: 2909 3700 4082 4717 275
Size: 791 382 635 1451
Fragments arranged by size:
1451 936 791 665 635 568 382 186
83 77 66 53
BsaWI w'CCGG_w
Cuts at: 2866 4326 4724 4998 5145 5556 2866
Size: 1460 398 274 147 411 3203
Fragments arranged by size:
3203 1460 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2661 2691 2765 2795 1178
Size: 30 165 30 1258 30 74 30 4276
Fragments arranged by size:
4276 1258 165 74 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2288 3152 3194 1186
Size: 1102 864 42 3885
Fragments arranged by size:
3885 1102 864 42
BseYI C'CCAG_C
Cuts at: 2388 2891 5096 5592 2388
Size: 503 2205 496 2689
Fragments arranged by size:
2689 2205 503 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2645 3077 1163
Size: 573 909 432 3979
Fragments arranged by size:
3979 909 573 432
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2293 2470 2688 3343 4104 5132
Size: 275 186 292 177 218 655 761 1028
Cuts at: 5132 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 655 292 275 218 186
177
BsiHKAI G_wGCw'C
Cuts at: 3954 4039 5110 3954
Size: 85 1071 4737
Fragments arranged by size:
4737 1071 85
BsmI GAATG_Cn'
Cuts at: 3404 3497 3404
Size: 93 5800
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2154 2216 2241 2712 3212 3954 4039
Size: 399 364 62 25 471 500 742 85
Cuts at: 4039 5110 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 500 471 399 364 85
62 25
BspEI T'CCGG_A
Cuts at: 5556 5556
Size: 5893
BspHI T'CATG_A
Cuts at: 3782 5512 3782
Size: 1730 4163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3248 844
Size: 2404 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2167 2320 2634 2641 2831 2986 3780
Size: 429 344 153 314 7 190 155 794
Cuts at: 3780 1394
Size: 3507
Fragments arranged by size:
3507 794 429 344 314 190 155 153
7
BsrDI GCAATG_nn'
Cuts at: 65 1248 2337 4388 4562 65
Size: 1183 1089 2051 174 1396
Fragments arranged by size:
2051 1396 1183 1089 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2089 2097 2510 2716 2839 2866 2880
Size: 110 279 8 413 206 123 27 14
Cuts at: 2880 4537 4724 1700
Size: 1657 187 2869
Fragments arranged by size:
2869 1657 413 279 206 187 123 110
27 14 8
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5893
BssHII G'CGCG_C
Cuts at: 1925 2111 2492 2876 2936 1925
Size: 186 381 384 60 4882
Fragments arranged by size:
4882 384 381 186 60
BssSI C'ACGA_G
Cuts at: 2123 2234 3953 4965 2123
Size: 111 1719 1012 3051
Fragments arranged by size:
3051 1719 1012 111
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2125 2149 794
Size: 1331 24 4538
Fragments arranged by size:
4538 1331 24
BstBI TT'CG_AA
Cuts at: 4697 4697
Size: 5893
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5893
BstXI CCAn_nnnn'nTGG
Cuts at: 3137 3137
Size: 5893
BstYI r'GATC_y
Cuts at: 1972 3223 3590 3975 3992 5444 5530 5542 5888
Size: 1251 367 385 17 1452 86 12 346
Cuts at: 5888 1972
Size: 1977
Fragments arranged by size:
1977 1452 1251 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2665 2967 513
Size: 552 939 661 302 3439
Fragments arranged by size:
3439 939 661 552 302
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5154
Fragments arranged by size:
5154 683 56
BtsI GCAGTG_nn'
Cuts at: 2508 3409 4203 4223 2508
Size: 901 794 20 4178
Fragments arranged by size:
4178 901 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5893
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5858
DraI TTT'AAA
Cuts at: 3197 3197
Size: 5893
DraIII CAC_nnn'GTG
Cuts at: 2821 2821
Size: 5893
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4900 809
Size: 507 3584 1802
Fragments arranged by size:
3584 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2095 2334 2401
Size: 54 1425 50 275 18 265 239 67
Cuts at: 2401 2685 2930 3340 4229 5858 8
Size: 284 245 410 889 1629 43
Fragments arranged by size:
1629 1425 889 410 284 275 265 245
239 67 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2685 3340 1537
Size: 275 873 655 4090
Fragments arranged by size:
4090 873 655 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2732 3823 1353
Size: 1379 1091 3423
Fragments arranged by size:
3423 1379 1091
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4480 4854 5000 387
Size: 1064 138 2891 374 146 1280
Fragments arranged by size:
2891 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3065 3956 4553 5340 5608 1239
Size: 393 1433 891 597 787 268 1524
Fragments arranged by size:
1524 1433 891 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2230 2230
Size: 5893
EcoRI G'AATT_C
Cuts at: 3202 3580 3202
Size: 378 5515
FspI TGC'GCA
Cuts at: 4399 4399
Size: 5893
HaeII r_GCGC'y
Cuts at: 1678 2057 2104 2181 2224 2752 2846 2912 3110
Size: 379 47 77 43 528 94 66 198
Cuts at: 3110 5040 1678
Size: 1930 2531
Fragments arranged by size:
2531 1930 528 379 198 94 77 66
47 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4534 4566 4608 4640 1373
Size: 32 135 32 2962 32 42 32 2626
Fragments arranged by size:
2962 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3237 3483 669
Size: 988 281 1299 246 3079
Fragments arranged by size:
3079 1299 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5893
HpaI GTT'AAC
Cuts at: 3483 3483
Size: 5893
KasI G'GCGC_C
Cuts at: 2100 2177 2842 2908 2100
Size: 77 665 66 5085
Fragments arranged by size:
5085 665 77 66
KpnI G_GTAC'C
Cuts at: 3218 3218
Size: 5893
MfeI C'AATT_G
Cuts at: 3492 3492
Size: 5893
MluI A'CGCG_T
Cuts at: 5769 5769
Size: 5893
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5007 5191 1305
Size: 3702 184 2007
Fragments arranged by size:
3702 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2336 10
Size: 54 1425 343 504 3567
Fragments arranged by size:
3567 1425 504 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2342 2717 3851 4210 4369 518
Size: 648 1176 375 1134 359 159 2042
Fragments arranged by size:
2042 1176 1134 648 375 359 159
MspA1I CmG'CkG
Cuts at: 1606 2192 2667 3309 3986 5134 5379 1606
Size: 586 475 642 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 642 586 475 245
NaeI GCC'GGC
Cuts at: 2099 2512 2841 2882 2099
Size: 413 329 41 5110
Fragments arranged by size:
5110 413 329 41
NarI GG'CG_CC
Cuts at: 2101 2178 2843 2909 2101
Size: 77 665 66 5085
Fragments arranged by size:
5085 665 77 66
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4402
Fragments arranged by size:
4402 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5893
NgoMIV G'CCGG_C
Cuts at: 2097 2510 2839 2880 2097
Size: 413 329 41 5110
Fragments arranged by size:
5110 413 329 41
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5893
NotI GC'GGCC_GC
Cuts at: 3340 3340
Size: 5893
NsiI A_TGCA'T
Cuts at: 3147 3147
Size: 5893
NspI r_CATG'y
Cuts at: 1132 1229 2356 3145 3251 4796 1132
Size: 97 1127 789 106 1545 2229
Fragments arranged by size:
2229 1545 1127 789 106 97
PasI CC'CwG_GG
Cuts at: 1348 2173 1348
Size: 825 5068
PciI A'CATG_T
Cuts at: 2352 4792 2352
Size: 2440 3453
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5893
PfoI T'CCnGG_A
Cuts at: 2226 2605 2730 2226
Size: 379 125 5389
Fragments arranged by size:
5389 379 125
PmeI GTTT'AAAC
Cuts at: 3197 3197
Size: 5893
PmlI CAC'GTG
Cuts at: 2554 2554
Size: 5893
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3962 3994 5475 5507 3962
Size: 32 1481 32 4348
Fragments arranged by size:
4348 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2230 2230
Size: 5893
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5893
PsiI TTA'TAA
Cuts at: 3463 3463
Size: 5893
PspXI vC'TCGA_Gb
Cuts at: 2832 2832
Size: 5893
PstI C_TGCA'G
Cuts at: 830 3245 830
Size: 2415 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5893
RsrII CG'GwC_CG
Cuts at: 2584 2584
Size: 5893
SacII CC_GC'GG
Cuts at: 2668 2668
Size: 5893
SalI G'TCGA_C
Cuts at: 1936 3235 1936
Size: 1299 4594
SapI GCTCTTCn'nnn_
Cuts at: 2732 2732
Size: 5893
SbfI CC_TGCA'GG
Cuts at: 3245 3245
Size: 5893
ScaI AGT'ACT
Cuts at: 4141 5853 4141
Size: 1712 4181
SexAI A'CCwGG_T
Cuts at: 2817 2817
Size: 5893
SfcI C'TryA_G
Cuts at: 826 1045 3241 4376 5057 5248 826
Size: 219 2196 1135 681 191 1471
Fragments arranged by size:
2196 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2102 2179 2844 2910 2102
Size: 77 665 66 5085
Fragments arranged by size:
5085 665 77 66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
SgrAI Cr'CCGG_yG
Cuts at: 1700 2716 2866 1700
Size: 1016 150 4727
Fragments arranged by size:
4727 1016 150
SmaI CCC'GGG
Cuts at: 1872 3220 1872
Size: 1348 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2832 3082 3997 4898 5160 820
Size: 197 926 889 250 915 901 262 1553
Fragments arranged by size:
1553 926 915 901 889 262 250 197
SnaBI TAC'GTA
Cuts at: 493 3684 493
Size: 3191 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5893
SphI G_CATG'C
Cuts at: 1132 3145 3251 1132
Size: 2013 106 3774
Fragments arranged by size:
3774 2013 106
SspI AAT'ATT
Cuts at: 5 52 3817 5
Size: 47 3765 2081
Fragments arranged by size:
3765 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5893
StyI C'CwwG_G
Cuts at: 513 1065 2004 3332 513
Size: 552 939 1328 3074
Fragments arranged by size:
3074 1328 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2318 2548 2792 3920 3937 4090 4275
Size: 1255 738 230 244 1128 17 153 185
Cuts at: 4275 4720 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1128 738 445 244 230 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4139 5851 96
Size: 274 80 33 51 1443 2162 1712 138
Fragments arranged by size:
2162 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2568 2600 2765 2797 5853 5885 2568
Size: 32 165 32 3056 32 2576
Fragments arranged by size:
3056 2576 165 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2522 1316
Size: 1206 4687
XbaI T'CTAG_A
Cuts at: 3229 3229
Size: 5893
XhoI C'TCGA_G
Cuts at: 1943 2832 1943
Size: 889 5004
XmaI C'CCGG_G
Cuts at: 1870 3218 1870
Size: 1348 4545
XmnI GAAnn'nnTTC
Cuts at: 4022 4022
Size: 5893
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3701 276
Size: 53 83 186 936 2167 2468
Fragments arranged by size:
2468 2167 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AvrII BaeI BbvCI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI
FspAI NruI PacI PspOMI PsrI PvuII SacI SanDI
SfiI SrfI SwaI XcmI