Restriction map for
pFN21AE1872
(Circular) (Six-base) MAPSORT of: pFN21AE1872.seq Check: 9134 from: 1 to: 5884
pFN21AE1872 5884 bp
With 182 enzymes: SgfI *
March 28, 2012 13:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2761 2761
Size: 5884
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3694 278
Size: 53 83 186 936 2158 2468
Fragments arranged by size:
2468 2158 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3227 1937
Size: 1290 4594
Acc65I G'GTAC_C
Cuts at: 3205 3205
Size: 5884
AclI AA'CG_TT
Cuts at: 1563 4011 4384 1563
Size: 2448 373 3063
Fragments arranged by size:
3063 2448 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2956 3947 5331 5599 1632
Size: 1324 991 1384 268 1917
Fragments arranged by size:
1917 1384 1324 991 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5687
AflIII A'CryG_T
Cuts at: 1203 4783 5760 1203
Size: 3580 977 1327
Fragments arranged by size:
3580 1327 977
AgeI A'CCGG_T
Cuts at: 4715 4715
Size: 5884
AhdI GACnn_n'nnGTC
Cuts at: 4613 4613
Size: 5884
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5884
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3141 1184
Size: 1957 3927
AlwNI CAG_nnn'CTG
Cuts at: 2347 2753 2776 2815 3019 3627 5199 2347
Size: 406 23 39 204 608 1572 3032
Fragments arranged by size:
3032 1572 608 406 204 39 23
ApaI G_GGCC'C
Cuts at: 3026 3026
Size: 5884
ApaLI G'TGCA_C
Cuts at: 2262 3941 5097 2262
Size: 1679 1156 3049
Fragments arranged by size:
3049 1679 1156
ApoI r'AATT_y
Cuts at: 1493 2282 3052 3193 3419 3571 5680 1493
Size: 789 770 141 226 152 2109 1697
Fragments arranged by size:
2109 1697 789 770 226 152 141
AseI AT'TA_AT
Cuts at: 160 1032 4438 160
Size: 872 3406 1606
Fragments arranged by size:
3406 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
AvaI C'yCGr_G
Cuts at: 1870 1943 2859 3209 1870
Size: 73 916 350 4545
Fragments arranged by size:
4545 916 350 73
BamHI G'GATC_C
Cuts at: 3214 3581 3214
Size: 367 5517
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3205 4660 618
Size: 325 217 627 1418 1455 1842
Fragments arranged by size:
1842 1455 1418 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2328 2745 2805 2970 3026 3203 1391
Size: 937 417 60 165 56 177 4072
Fragments arranged by size:
4072 937 417 177 165 60 56
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2252 928
Size: 971 353 4560
Fragments arranged by size:
4560 971 353
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4075 4109 1903
Size: 34 2138 34 3678
Fragments arranged by size:
3678 2138 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3776 4986 3776
Size: 1210 4674
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5884
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4495 136
Size: 107 122 71 631 605 2823 1525
Fragments arranged by size:
2823 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5879 5879
Size: 5884
BlpI GC'TnA_GC
Cuts at: 2322 2739 2819 3301 2322
Size: 417 80 482 4905
Fragments arranged by size:
4905 482 417 80
Bme1580I G_kGCm'C
Cuts at: 1790 2023 2266 3026 3945 5101 1790
Size: 233 243 760 919 1156 2573
Fragments arranged by size:
2573 1156 919 760 243 233
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5884
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2056 3615 4573
Size: 416 53 83 399 24 639 1559 958
Cuts at: 4573 442
Size: 1753
Fragments arranged by size:
1753 1559 958 639 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2465 2628 2648 2819 3061 3135 3161 4544
Size: 1226 163 20 171 242 74 26 1383
Cuts at: 4544 1239
Size: 2579
Fragments arranged by size:
2579 1383 1226 242 171 163 74 26
20
Bpu10I CC'TnA_GC
Cuts at: 2568 2568
Size: 5884
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2849 4009 4874 5172 2849
Size: 1160 865 298 3561
Fragments arranged by size:
3561 1160 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4547 882
Size: 617 3048 2219
Fragments arranged by size:
3048 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3675 493
Size: 711 2471 2702
Fragments arranged by size:
2702 2471 711
BsaBI GATnn'nnATC
Cuts at: 5878 5878
Size: 5884
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3691 4073 4708 275
Size: 53 83 186 936 2158 382 635 1451
Fragments arranged by size:
2158 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2040 2118 4317 4715 4989 5136 5547 2040
Size: 78 2199 398 274 147 411 2377
Fragments arranged by size:
2377 2199 411 398 274 147 78
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5659
Fragments arranged by size:
5659 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2516 2519 1186
Size: 1330 3 4551
Fragments arranged by size:
4551 1330 3
BseYI C'CCAG_C
Cuts at: 2091 2948 5087 5583 2091
Size: 857 2139 496 2392
Fragments arranged by size:
2392 2139 857 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2011 2443 2894 1163
Size: 573 275 432 451 4153
Fragments arranged by size:
4153 573 451 432 275
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3334 4095 5123 1540
Size: 275 186 1333 761 1028 2301
Fragments arranged by size:
2301 1333 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2266 2481 2970 3945 4030 5101 2266
Size: 215 489 975 85 1071 3049
Fragments arranged by size:
3049 1071 975 489 215 85
BsmI GAATG_Cn'
Cuts at: 2911 3395 3488 2911
Size: 484 93 5307
Fragments arranged by size:
5307 484 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2368 2368
Size: 5884
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2266 2328 2481 2745 2805 2970
Size: 399 233 243 62 153 264 60 165
Cuts at: 2970 3026 3203 3945 4030 5101 1391
Size: 56 177 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 264 243 233 177
165 153 85 62 60 56
BspEI T'CCGG_A
Cuts at: 2040 2118 5547 2040
Size: 78 3429 2377
Fragments arranged by size:
3429 2377 78
BspHI T'CATG_A
Cuts at: 3773 5503 3773
Size: 1730 4154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2761 3239 844
Size: 1917 478 3489
Fragments arranged by size:
3489 1917 478
BsrBI CCG'CTC
Cuts at: 1394 1823 3771 1394
Size: 429 1948 3507
Fragments arranged by size:
3507 1948 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4379 4553 65
Size: 1183 3131 174 1396
Fragments arranged by size:
3131 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4528 4715 1700
Size: 110 2718 187 2869
Fragments arranged by size:
2869 2718 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5884
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5884
BssSI C'ACGA_G
Cuts at: 3944 4956 3944
Size: 1012 4872
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2815 2944 2958 794
Size: 2021 129 14 3720
Fragments arranged by size:
3720 2021 129 14
BstBI TT'CG_AA
Cuts at: 4688 4688
Size: 5884
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5884
BstXI CCAn_nnnn'nTGG
Cuts at: 2241 2241
Size: 5884
BstYI r'GATC_y
Cuts at: 1972 3214 3581 3966 3983 5435 5521 5533 5879
Size: 1242 367 385 17 1452 86 12 346
Cuts at: 5879 1972
Size: 1977
Fragments arranged by size:
1977 1452 1242 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2746 2746
Size: 5884
BtgI C'CryG_G
Cuts at: 513 1065 2004 3070 513
Size: 552 939 1066 3327
Fragments arranged by size:
3327 1066 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5145
Fragments arranged by size:
5145 683 56
BtsI GCAGTG_nn'
Cuts at: 2660 3400 4194 4214 2660
Size: 740 794 20 4330
Fragments arranged by size:
4330 794 740 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5884
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5849
DraI TTT'AAA
Cuts at: 3188 3188
Size: 5884
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4891 809
Size: 507 3575 1802
Fragments arranged by size:
3575 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2368 2847 3331
Size: 54 1425 50 275 18 538 479 484
Cuts at: 3331 4220 5849 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 538 484 479 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3331 1537
Size: 275 1519 4090
Fragments arranged by size:
4090 1519 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2433 2873 2975 3057 3814 1353
Size: 1080 440 102 82 757 3423
Fragments arranged by size:
3423 1080 757 440 102 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4471 4845 4991 387
Size: 1064 138 2882 374 146 1280
Fragments arranged by size:
2882 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2968 2968
Size: 5884
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2465 2628 2648 2819 2956 3061 3135
Size: 393 833 163 20 171 137 105 74
Cuts at: 3135 3161 3947 4544 5331 5599 1239
Size: 26 786 597 787 268 1524
Fragments arranged by size:
1524 833 787 786 597 393 268 171
163 137 105 74 26 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2425 2928 2425
Size: 503 5381
EcoO109I rG'GnC_Cy
Cuts at: 2599 2622 3016 3023 2599
Size: 23 394 7 5460
Fragments arranged by size:
5460 394 23 7
EcoRI G'AATT_C
Cuts at: 3193 3571 3193
Size: 378 5506
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2167 2167
Size: 5884
FspI TGC'GCA
Cuts at: 4390 4390
Size: 5884
HaeII r_GCGC'y
Cuts at: 1678 2815 2881 5031 1678
Size: 1137 66 2150 2531
Fragments arranged by size:
2531 2150 1137 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2606 2638 2985 3017 4525
Size: 32 135 32 1034 32 347 32 1508
Cuts at: 4525 4557 4599 4631 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1508 1034 347 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3228 3474 669
Size: 988 281 1290 246 3079
Fragments arranged by size:
3079 1290 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5884
HpaI GTT'AAC
Cuts at: 3474 3474
Size: 5884
KpnI G_GTAC'C
Cuts at: 3209 3209
Size: 5884
MfeI C'AATT_G
Cuts at: 3483 3483
Size: 5884
MluI A'CGCG_T
Cuts at: 5760 5760
Size: 5884
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4998 5182 1305
Size: 3693 184 2007
Fragments arranged by size:
3693 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2370 2849 10
Size: 54 1425 343 538 479 3045
Fragments arranged by size:
3045 1425 538 479 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2843 3842 4201 4360 518
Size: 648 1677 999 359 159 2042
Fragments arranged by size:
2042 1677 999 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2237 3300 3977 5125 5370 1606
Size: 631 1063 677 1148 245 2120
Fragments arranged by size:
2120 1148 1063 677 631 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3070 513
Size: 552 939 1066 3327
Fragments arranged by size:
3327 1066 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5884
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5884
NotI GC'GGCC_GC
Cuts at: 3331 3331
Size: 5884
NspI r_CATG'y
Cuts at: 1132 1229 2138 3242 4787 1132
Size: 97 909 1104 1545 2229
Fragments arranged by size:
2229 1545 1104 909 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5884
PciI A'CATG_T
Cuts at: 4783 4783
Size: 5884
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2901 1108
Size: 1793 4091
PfoI T'CCnGG_A
Cuts at: 2080 2154 2481 2900 2080
Size: 74 327 419 5064
Fragments arranged by size:
5064 419 327 74
PmeI GTTT'AAAC
Cuts at: 3188 3188
Size: 5884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3953 3985 5466 5498 3953
Size: 32 1481 32 4339
Fragments arranged by size:
4339 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2622 2622
Size: 5884
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5884
PsiI TTA'TAA
Cuts at: 3454 3454
Size: 5884
PspOMI G'GGCC_C
Cuts at: 3022 3022
Size: 5884
PstI C_TGCA'G
Cuts at: 830 2993 3236 830
Size: 2163 243 3478
Fragments arranged by size:
3478 2163 243
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5884
PvuII CAG'CTG
Cuts at: 2237 2237
Size: 5884
SacI G_AGCT'C
Cuts at: 2970 2970
Size: 5884
SalI G'TCGA_C
Cuts at: 1936 3226 1936
Size: 1290 4594
SapI GCTCTTCn'nnn_
Cuts at: 2975 2975
Size: 5884
SbfI CC_TGCA'GG
Cuts at: 3236 3236
Size: 5884
ScaI AGT'ACT
Cuts at: 4132 5844 4132
Size: 1712 4172
SfcI C'TryA_G
Cuts at: 826 1045 2989 3232 4367 5048 5239 826
Size: 219 1944 243 1135 681 191 1471
Fragments arranged by size:
1944 1471 1135 681 243 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5884
SmaI CCC'GGG
Cuts at: 1872 3211 1872
Size: 1339 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2828 3988 4889 5151 820
Size: 197 926 885 1160 901 262 1553
Fragments arranged by size:
1553 1160 926 901 885 262 197
SnaBI TAC'GTA
Cuts at: 493 3675 493
Size: 3182 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5884
SphI G_CATG'C
Cuts at: 1132 3242 1132
Size: 2110 3774
SspI AAT'ATT
Cuts at: 5 52 2196 3808 5
Size: 47 2144 1612 2081
Fragments arranged by size:
2144 2081 1612 47
StuI AGG'CCT
Cuts at: 1517 2186 2865 3122 1517
Size: 669 679 257 4279
Fragments arranged by size:
4279 679 669 257
StyI C'CwwG_G
Cuts at: 513 1065 2004 3070 3323 513
Size: 552 939 1066 253 3074
Fragments arranged by size:
3074 1066 939 552 253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2038 3911 3928 4081 4266 4711 325
Size: 1255 458 1873 17 153 185 445 1498
Fragments arranged by size:
1873 1498 1255 458 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4130 5842 96
Size: 274 80 33 51 1443 2153 1712 138
Fragments arranged by size:
2153 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2215 2247 2452 2484 3021 3053 5844 5876 2215
Size: 32 205 32 537 32 2791 32 2223
Fragments arranged by size:
2791 2223 537 205 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2999 1316
Size: 1683 4201
XbaI T'CTAG_A
Cuts at: 3220 3220
Size: 5884
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2537 2921 3175 2537
Size: 384 254 5246
Fragments arranged by size:
5246 384 254
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5884
XmaI C'CCGG_G
Cuts at: 1870 3209 1870
Size: 1339 4545
XmnI GAAnn'nnTTC
Cuts at: 4013 4013
Size: 5884
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3692 276
Size: 53 83 186 936 2158 2468
Fragments arranged by size:
2468 2158 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI