Restriction map for
pFN21AE1869
(Circular) (Six-base) MAPSORT of: pFN21AE1869.seq Check: 5660 from: 1 to: 5875
pFN21AE1869 5875 bp
With 182 enzymes: SgfI *
December 12, 2011 16:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2108 2108
Size: 5875
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3685 278
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3218 1937
Size: 1281 4594
Acc65I G'GTAC_C
Cuts at: 2434 3196 2434
Size: 762 5113
AclI AA'CG_TT
Cuts at: 1563 4002 4375 1563
Size: 2439 373 3063
Fragments arranged by size:
3063 2439 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2845 3938 5322 5590 1632
Size: 1213 1093 1384 268 1917
Fragments arranged by size:
1917 1384 1213 1093 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5678
AflIII A'CryG_T
Cuts at: 1203 4774 5751 1203
Size: 3571 977 1327
Fragments arranged by size:
3571 1327 977
AgeI A'CCGG_T
Cuts at: 4706 4706
Size: 5875
AhdI GACnn_n'nnGTC
Cuts at: 4604 4604
Size: 5875
AleI CACnn'nnGTG
Cuts at: 1166 2333 1166
Size: 1167 4708
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2576 1184
Size: 1392 4483
AlwNI CAG_nnn'CTG
Cuts at: 2164 2815 3085 3618 5190 2164
Size: 651 270 533 1572 2849
Fragments arranged by size:
2849 1572 651 533 270
ApaI G_GGCC'C
Cuts at: 2809 2979 2809
Size: 170 5705
ApaLI G'TGCA_C
Cuts at: 3932 5088 3932
Size: 1156 4719
ApoI r'AATT_y
Cuts at: 1493 3184 3410 3562 5671 1493
Size: 1691 226 152 2109 1697
Fragments arranged by size:
2109 1697 1691 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4429 160
Size: 872 3397 1606
Fragments arranged by size:
3397 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
AvaI C'yCGr_G
Cuts at: 1870 1943 2191 2225 2294 2457 2972 3046 3200
Size: 73 248 34 69 163 515 74 154
Cuts at: 3200 1870
Size: 4545
Fragments arranged by size:
4545 515 248 163 154 74 73 69
34
BamHI G'GATC_C
Cuts at: 2934 3205 3572 2934
Size: 271 367 5237
Fragments arranged by size:
5237 367 271
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2206 2434 2566 2829 3050
Size: 325 217 627 419 228 132 263 221
Cuts at: 3050 3061 3196 4651 618
Size: 11 135 1455 1842
Fragments arranged by size:
1842 1455 627 419 325 263 228 221
217 135 132 11
BanII G_rGCy'C
Cuts at: 1391 2295 2424 2809 2842 2946 2979 3194 1391
Size: 904 129 385 33 104 33 215 4072
Fragments arranged by size:
4072 904 385 215 129 104 33 33
BbeI G_GCGC'C
Cuts at: 3054 3065 3054
Size: 11 5864
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4904
BbvCI CC'TCA_GC
Cuts at: 2477 2549 2477
Size: 72 5803
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2092 2126 2902 2936 4066
Size: 34 50 34 71 34 776 34 1130
Cuts at: 4066 4100 1903
Size: 34 3678
Fragments arranged by size:
3678 1130 776 71 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3767 4977 3767
Size: 1210 4665
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5875
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2017 2077 3059
Size: 107 122 71 631 605 345 60 982
Cuts at: 3059 4486 136
Size: 1427 1525
Fragments arranged by size:
1525 1427 982 631 605 345 122 107
71 60
BglII A'GATC_T
Cuts at: 5870 5870
Size: 5875
BlpI GC'TnA_GC
Cuts at: 3292 3292
Size: 5875
Bme1580I G_kGCm'C
Cuts at: 1790 2809 2979 3936 5092 1790
Size: 1019 170 957 1156 2573
Fragments arranged by size:
2573 1156 1019 957 170
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5875
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3606 4564 442
Size: 416 53 83 399 24 2189 958 1753
Fragments arranged by size:
2189 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2819 3107 4535 1239
Size: 1580 288 1428 2579
Fragments arranged by size:
2579 1580 1428 288
Bpu10I CC'TnA_GC
Cuts at: 2477 2549 2660 2477
Size: 72 111 5692
Fragments arranged by size:
5692 111 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2468 4000 4865 5163 2468
Size: 1532 865 298 3180
Fragments arranged by size:
3180 1532 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4538 882
Size: 617 3039 2219
Fragments arranged by size:
3039 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2506 3112 3666 493
Size: 711 1302 606 554 2702
Fragments arranged by size:
2702 1302 711 606 554
BsaBI GATnn'nnATC
Cuts at: 5869 5869
Size: 5875
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2069 3051 3062 3682
Size: 53 83 186 936 536 982 11 620
Cuts at: 3682 4064 4699 275
Size: 382 635 1451
Fragments arranged by size:
1451 982 936 635 620 536 382 186
83 53 11
BsaWI w'CCGG_w
Cuts at: 2287 2606 4308 4706 4980 5127 5538 2287
Size: 319 1702 398 274 147 411 2624
Fragments arranged by size:
2624 1702 411 398 319 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3125 3155 1178
Size: 30 165 30 1722 30 3898
Fragments arranged by size:
3898 1722 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5875
BseYI C'CCAG_C
Cuts at: 2570 2808 5078 5574 2570
Size: 238 2270 496 2871
Fragments arranged by size:
2871 2270 496 238
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2301 3190 1163
Size: 573 565 889 3848
Fragments arranged by size:
3848 889 573 565
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2081 2085 2263 2731 3325 4086
Size: 275 186 80 4 178 468 594 761
Cuts at: 4086 5114 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 594 468 275 186 178
80 4
BsiHKAI G_wGCw'C
Cuts at: 2238 2424 3936 4021 5092 2238
Size: 186 1512 85 1071 3021
Fragments arranged by size:
3021 1512 1071 186 85
BsmI GAATG_Cn'
Cuts at: 3386 3479 3386
Size: 93 5782
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2238 2295 2424 2809 2842 2946 2979
Size: 399 448 57 129 385 33 104 33
Cuts at: 2979 3194 3936 4021 5092 1391
Size: 215 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 448 399 385 215 129
104 85 57 33 33
BspEI T'CCGG_A
Cuts at: 5538 5538
Size: 5875
BspHI T'CATG_A
Cuts at: 3764 5494 3764
Size: 1730 4145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2108 3230 844
Size: 1264 1122 3489
Fragments arranged by size:
3489 1264 1122
BsrBI CCG'CTC
Cuts at: 1394 1823 3762 1394
Size: 429 1939 3507
Fragments arranged by size:
3507 1939 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4370 4544 65
Size: 1183 3122 174 1396
Fragments arranged by size:
3122 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2076 2080 2556 2726 2766 4519 4706
Size: 110 266 4 476 170 40 1753 187
Cuts at: 4706 1700
Size: 2869
Fragments arranged by size:
2869 1753 476 266 187 170 110 40
4
BsrGI T'GTAC_A
Cuts at: 96 2244 2898 3030 96
Size: 2148 654 132 2941
Fragments arranged by size:
2941 2148 654 132
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5875
BssSI C'ACGA_G
Cuts at: 2939 3935 4947 2939
Size: 996 1012 3867
Fragments arranged by size:
3867 1012 996
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5875
BstBI TT'CG_AA
Cuts at: 4679 4679
Size: 5875
BstEII G'GTnAC_C
Cuts at: 1184 2326 2738 1184
Size: 1142 412 4321
Fragments arranged by size:
4321 1142 412
BstXI CCAn_nnnn'nTGG
Cuts at: 2797 2797
Size: 5875
BstYI r'GATC_y
Cuts at: 1972 2160 2934 3205 3572 3957 3974 5426 5512
Size: 188 774 271 367 385 17 1452 86
Cuts at: 5512 5524 5870 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 774 385 367 346 271 188
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2301 2301
Size: 5875
BtgI C'CryG_G
Cuts at: 513 1065 2085 2152 2632 2868 513
Size: 552 1020 67 480 236 3520
Fragments arranged by size:
3520 1020 552 480 236 67
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5136
Fragments arranged by size:
5136 683 56
BtsI GCAGTG_nn'
Cuts at: 2627 2668 2696 3391 4185 4205 2627
Size: 41 28 695 794 20 4297
Fragments arranged by size:
4297 794 695 41 28 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5875
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5840
DraI TTT'AAA
Cuts at: 3179 3179
Size: 5875
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4882 809
Size: 507 3566 1802
Fragments arranged by size:
3566 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2074 2078 2082
Size: 54 1425 50 275 18 244 4 4
Cuts at: 2082 2155 2268 2728 2745 2787 3322 4211 5840
Size: 73 113 460 17 42 535 889 1629
Cuts at: 5840 8
Size: 43
Fragments arranged by size:
1629 1425 889 535 460 275 244 113
73 54 50 43 42 18 17 4
4
EagI C'GGCC_G
Cuts at: 1537 1812 2078 2082 2728 3322 1537
Size: 275 266 4 646 594 4090
Fragments arranged by size:
4090 646 594 275 266 4
EarI CTCTTCn'nnn_
Cuts at: 1353 3805 1353
Size: 2452 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4462 4836 4982 387
Size: 1064 138 2873 374 146 1280
Fragments arranged by size:
2873 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2422 2422
Size: 5875
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2819 2845 3107 3938 4535 5322 5590
Size: 393 1187 26 262 831 597 787 268
Cuts at: 5590 1239
Size: 1524
Fragments arranged by size:
1524 1187 831 787 597 393 268 262
26
EcoO109I rG'GnC_Cy
Cuts at: 2805 2917 2976 3019 2805
Size: 112 59 43 5661
Fragments arranged by size:
5661 112 59 43
EcoRI G'AATT_C
Cuts at: 3184 3562 3184
Size: 378 5497
FseI GG_CCGG'CC
Cuts at: 2080 2084 2080
Size: 4 5871
FspI TGC'GCA
Cuts at: 4381 4381
Size: 5875
HaeII r_GCGC'y
Cuts at: 1678 2667 3054 3065 3086 5022 1678
Size: 989 387 11 21 1936 2531
Fragments arranged by size:
2531 1936 989 387 21 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2576 2608 3125 3157 4516
Size: 32 135 32 1004 32 517 32 1359
Cuts at: 4516 4548 4590 4622 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1359 1004 517 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3219 3465 669
Size: 988 281 1281 246 3079
Fragments arranged by size:
3079 1281 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5875
HpaI GTT'AAC
Cuts at: 3465 3465
Size: 5875
KasI G'GCGC_C
Cuts at: 3050 3061 3050
Size: 11 5864
KpnI G_GTAC'C
Cuts at: 2438 3200 2438
Size: 762 5113
MfeI C'AATT_G
Cuts at: 3474 3474
Size: 5875
MluI A'CGCG_T
Cuts at: 5751 5751
Size: 5875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3168 4989 5173 1305
Size: 1863 1821 184 2007
Fragments arranged by size:
2007 1863 1821 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2747 2789 10
Size: 54 1425 343 915 42 3096
Fragments arranged by size:
3096 1425 915 343 54 42
MslI CAynn'nnrTG
Cuts at: 518 1166 2333 2399 3152 3833 4192 4351 518
Size: 648 1167 66 753 681 359 159 2042
Fragments arranged by size:
2042 1167 753 681 648 359 159 66
MspA1I CmG'CkG
Cuts at: 1606 2047 2087 2154 2221 2797 2896 3094 3139
Size: 441 40 67 67 576 99 198 45
Cuts at: 3139 3291 3968 5116 5361 1606
Size: 152 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 576 441 245 198 152
99 67 67 45 40
NaeI GCC'GGC
Cuts at: 2078 2082 2078
Size: 4 5871
NarI GG'CG_CC
Cuts at: 3051 3062 3051
Size: 11 5864
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5323
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5875
NgoMIV G'CCGG_C
Cuts at: 2076 2080 2076
Size: 4 5871
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5875
NotI GC'GGCC_GC
Cuts at: 3322 3322
Size: 5875
NspI r_CATG'y
Cuts at: 1132 1229 3233 4778 1132
Size: 97 2004 1545 2229
Fragments arranged by size:
2229 2004 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5875
PciI A'CATG_T
Cuts at: 4774 4774
Size: 5875
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5875
PmeI GTTT'AAAC
Cuts at: 3179 3179
Size: 5875
PmlI CAC'GTG
Cuts at: 2506 2506
Size: 5875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3944 3976 5457 5489 3944
Size: 32 1481 32 4330
Fragments arranged by size:
4330 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2917 3019 2917
Size: 102 5773
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5875
PsiI TTA'TAA
Cuts at: 3445 3445
Size: 5875
PspOMI G'GGCC_C
Cuts at: 2805 2975 2805
Size: 170 5705
PstI C_TGCA'G
Cuts at: 830 3227 830
Size: 2397 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5875
PvuII CAG'CTG
Cuts at: 2896 3139 2896
Size: 243 5632
SacI G_AGCT'C
Cuts at: 2424 2424
Size: 5875
SacII CC_GC'GG
Cuts at: 2088 2155 2088
Size: 67 5808
SalI G'TCGA_C
Cuts at: 1936 3217 1936
Size: 1281 4594
SbfI CC_TGCA'GG
Cuts at: 3227 3227
Size: 5875
ScaI AGT'ACT
Cuts at: 4123 5835 4123
Size: 1712 4163
SfcI C'TryA_G
Cuts at: 826 1045 3223 4358 5039 5230 826
Size: 219 2178 1135 681 191 1471
Fragments arranged by size:
2178 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 3052 3063 3052
Size: 11 5864
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5875
SmaI CCC'GGG
Cuts at: 1872 2459 3048 3202 1872
Size: 587 589 154 4545
Fragments arranged by size:
4545 589 587 154
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2483 3979 4880 5142 820
Size: 197 926 540 1496 901 262 1553
Fragments arranged by size:
1553 1496 926 901 540 262 197
SnaBI TAC'GTA
Cuts at: 493 3666 493
Size: 3173 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5875
SphI G_CATG'C
Cuts at: 1132 3233 1132
Size: 2101 3774
SspI AAT'ATT
Cuts at: 5 52 3799 5
Size: 47 3747 2081
Fragments arranged by size:
3747 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5875
StyI C'CwwG_G
Cuts at: 513 1065 2319 2946 3126 3314 513
Size: 552 1254 627 180 188 3074
Fragments arranged by size:
3074 1254 627 552 188 180
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2584 3902 3919 4072 4257 4702 325
Size: 1255 1004 1318 17 153 185 445 1498
Fragments arranged by size:
1498 1318 1255 1004 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2244 2384 2898
Size: 274 80 33 51 1443 267 140 514
Cuts at: 2898 3009 3030 3105 4121 5833 96
Size: 111 21 75 1016 1712 138
Fragments arranged by size:
1712 1443 1016 514 274 267 140 138
111 80 75 51 33 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5835 5867 5835
Size: 32 5843
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5875
XbaI T'CTAG_A
Cuts at: 3211 3211
Size: 5875
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5875
XmaI C'CCGG_G
Cuts at: 1870 2457 3046 3200 1870
Size: 587 589 154 4545
Fragments arranged by size:
4545 589 587 154
XmnI GAAnn'nnTTC
Cuts at: 4004 4004
Size: 5875
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3683 276
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoNI EcoRV FalI FspAI NruI
NsiI PacI PfoI PspXI PsrI RsrII SanDI SapI
SexAI SfiI SrfI SwaI XcmI