Restriction map for
pFN21AE1868
(Circular) (Six-base) MAPSORT of: pFN21AE1868.seq Check: 5664 from: 1 to: 5872
pFN21AE1868 5872 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3682 278
Size: 53 83 186 936 2146 2468
Fragments arranged by size:
2468 2146 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2931 3215 1937
Size: 994 284 4594
Fragments arranged by size:
4594 994 284
Acc65I G'GTAC_C
Cuts at: 3193 3193
Size: 5872
AclI AA'CG_TT
Cuts at: 1563 3999 4372 1563
Size: 2436 373 3063
Fragments arranged by size:
3063 2436 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2126 2207 2753 3935 5319 5587 1632
Size: 494 81 546 1182 1384 268 1917
Fragments arranged by size:
1917 1384 1182 546 494 268 81
AfeI AGC'GCT
Cuts at: 2276 3092 2276
Size: 816 5056
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5675
AflIII A'CryG_T
Cuts at: 1203 4771 5748 1203
Size: 3568 977 1327
Fragments arranged by size:
3568 1327 977
AgeI A'CCGG_T
Cuts at: 4703 4703
Size: 5872
AhdI GACnn_n'nnGTC
Cuts at: 4601 4601
Size: 5872
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5872
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5872
AlwNI CAG_nnn'CTG
Cuts at: 2230 2503 3615 5187 2230
Size: 273 1112 1572 2915
Fragments arranged by size:
2915 1572 1112 273
ApaLI G'TGCA_C
Cuts at: 3929 5085 3929
Size: 1156 4716
ApoI r'AATT_y
Cuts at: 1493 3181 3407 3559 5668 1493
Size: 1688 226 152 2109 1697
Fragments arranged by size:
2109 1697 1688 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4426 160
Size: 872 3394 1606
Fragments arranged by size:
3394 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5872
AvaI C'yCGr_G
Cuts at: 1870 1943 2038 2508 2634 2664 3197 1870
Size: 73 95 470 126 30 533 4545
Fragments arranged by size:
4545 533 470 126 95 73 30
BamHI G'GATC_C
Cuts at: 3202 3569 3202
Size: 367 5505
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2709 3193 4648 618
Size: 325 217 627 922 484 1455 1842
Fragments arranged by size:
1842 1455 922 627 484 325 217
BanII G_rGCy'C
Cuts at: 1391 2019 2083 2981 3191 1391
Size: 628 64 898 210 4072
Fragments arranged by size:
4072 898 628 210 64
BbeI G_GCGC'C
Cuts at: 2713 2713
Size: 5872
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4901
BbvCI CC'TCA_GC
Cuts at: 2474 2474
Size: 5872
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4063 4097 1903
Size: 34 50 34 2042 34 3678
Fragments arranged by size:
3678 2042 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3764 4974 3764
Size: 1210 4662
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5872
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4483 136
Size: 107 122 71 631 605 2811 1525
Fragments arranged by size:
2811 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5867 5867
Size: 5872
BlpI GC'TnA_GC
Cuts at: 3289 3289
Size: 5872
Bme1580I G_kGCm'C
Cuts at: 1790 3063 3933 5089 1790
Size: 1273 870 1156 2573
Fragments arranged by size:
2573 1273 1156 870
BmgBI CAC'GTC
Cuts at: 1318 2206 1318
Size: 888 4984
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3603 4561 442
Size: 416 53 83 399 24 2186 958 1753
Fragments arranged by size:
2186 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5872
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2647 2647
Size: 5872
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2030 2809 2901 4532 1239
Size: 791 779 92 1631 2579
Fragments arranged by size:
2579 1631 791 779 92
Bpu10I CC'TnA_GC
Cuts at: 2165 2474 2165
Size: 309 5563
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2315 2318 2539 3997 4862 5160 2315
Size: 3 221 1458 865 298 3027
Fragments arranged by size:
3027 1458 865 298 221 3
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2057 2958 4535 882
Size: 617 558 901 1577 2219
Fragments arranged by size:
2219 1577 901 617 558
BsaAI yAC'GTr
Cuts at: 493 1204 3663 493
Size: 711 2459 2702
Fragments arranged by size:
2702 2459 711
BsaBI GATnn'nnATC
Cuts at: 5866 5866
Size: 5872
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2213 2710 3679 4061
Size: 53 83 186 936 680 497 969 382
Cuts at: 4061 4696 275
Size: 635 1451
Fragments arranged by size:
1451 969 936 680 635 497 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4305 4703 4977 5124 5535 4305
Size: 398 274 147 411 4642
Fragments arranged by size:
4642 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2913 2943 1178
Size: 30 165 30 1510 30 4107
Fragments arranged by size:
4107 1510 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 1186
Size: 841 5031
BseYI C'CCAG_C
Cuts at: 2381 2537 2561 2839 5075 5571 2381
Size: 156 24 278 2236 496 2682
Fragments arranged by size:
2682 2236 496 278 156 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2092 2216 1163
Size: 573 356 124 4819
Fragments arranged by size:
4819 573 356 124
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2024 2308 3322 4083 5111 1540
Size: 275 186 23 284 1014 761 1028 2301
Fragments arranged by size:
2301 1028 1014 761 284 275 186 23
BsiHKAI G_wGCw'C
Cuts at: 3072 3933 4018 5089 3072
Size: 861 85 1071 3855
Fragments arranged by size:
3855 1071 861 85
BsmI GAATG_Cn'
Cuts at: 2688 3383 3476 2688
Size: 695 93 5084
Fragments arranged by size:
5084 695 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2019 2083 2981 3063 3072 3191 3933
Size: 399 229 64 898 82 9 119 742
Cuts at: 3933 4018 5089 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 898 742 399 229 119 85
82 64 9
BspEI T'CCGG_A
Cuts at: 5535 5535
Size: 5872
BspHI T'CATG_A
Cuts at: 3761 5491 3761
Size: 1730 4142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3227 844
Size: 2383 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2836 3759 1394
Size: 429 1013 923 3507
Fragments arranged by size:
3507 1013 923 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2095 3097 4367 4541 65
Size: 1183 847 1002 1270 174 1396
Fragments arranged by size:
1396 1270 1183 1002 847 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4516 4703 1700
Size: 110 2706 187 2869
Fragments arranged by size:
2869 2706 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5872
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5872
BssSI C'ACGA_G
Cuts at: 2246 3081 3932 4944 2246
Size: 835 851 1012 3174
Fragments arranged by size:
3174 1012 851 835
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2263 3045 3067 794
Size: 1469 782 22 3599
Fragments arranged by size:
3599 1469 782 22
BstBI TT'CG_AA
Cuts at: 4676 4676
Size: 5872
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5872
BstYI r'GATC_y
Cuts at: 1972 3202 3569 3954 3971 5423 5509 5521 5867
Size: 1230 367 385 17 1452 86 12 346
Cuts at: 5867 1972
Size: 1977
Fragments arranged by size:
1977 1452 1230 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2024 2403 3055 513
Size: 552 959 379 652 3330
Fragments arranged by size:
3330 959 652 552 379
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2221 487
Size: 683 56 995 4138
Fragments arranged by size:
4138 995 683 56
BtsI GCAGTG_nn'
Cuts at: 2461 2820 3388 4182 4202 2461
Size: 359 568 794 20 4131
Fragments arranged by size:
4131 794 568 359 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5872
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5837
DraI TTT'AAA
Cuts at: 3176 3176
Size: 5872
DraIII CAC_nnn'GTG
Cuts at: 2145 3067 2145
Size: 922 4950
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4879 809
Size: 507 3563 1802
Fragments arranged by size:
3563 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2021 2133 2305
Size: 54 1425 50 275 18 191 112 172
Cuts at: 2305 3052 3319 4208 5837 8
Size: 747 267 889 1629 43
Fragments arranged by size:
1629 1425 889 747 275 267 191 172
112 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2021 2305 3319 1537
Size: 275 209 284 1014 4090
Fragments arranged by size:
4090 1014 284 275 209
EarI CTCTTCn'nnn_
Cuts at: 1353 3802 1353
Size: 2449 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2022 4459 4833 4979 387
Size: 1064 138 433 2437 374 146 1280
Fragments arranged by size:
2437 1280 1064 433 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 2126 2207 2753 2809 2901 3935
Size: 393 398 96 81 546 56 92 1034
Cuts at: 3935 4532 5319 5587 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1034 787 597 546 398 393 268
96 92 81 56
EcoRI G'AATT_C
Cuts at: 3181 3559 3181
Size: 378 5494
FspI TGC'GCA
Cuts at: 2195 4378 2195
Size: 2183 3689
HaeII r_GCGC'y
Cuts at: 1678 2278 2328 2713 3094 5019 1678
Size: 600 50 385 381 1925 2531
Fragments arranged by size:
2531 1925 600 385 381 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2913 2945 4513 4545 4587
Size: 32 135 32 1341 32 1568 32 42
Cuts at: 4587 4619 1373
Size: 32 2626
Fragments arranged by size:
2626 1568 1341 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3216 3462 669
Size: 988 281 1278 246 3079
Fragments arranged by size:
3079 1278 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5872
HpaI GTT'AAC
Cuts at: 3462 3462
Size: 5872
KasI G'GCGC_C
Cuts at: 2709 2709
Size: 5872
KpnI G_GTAC'C
Cuts at: 3197 3197
Size: 5872
MfeI C'AATT_G
Cuts at: 3471 3471
Size: 5872
MluI A'CGCG_T
Cuts at: 5748 5748
Size: 5872
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3006 4986 5170 1305
Size: 1701 1980 184 2007
Fragments arranged by size:
2007 1980 1701 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4050
Fragments arranged by size:
4050 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2587 3830 4189 4348 518
Size: 648 1421 1243 359 159 2042
Fragments arranged by size:
2042 1421 1243 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2026 2128 2263 2494 2769 3288 3965 5113
Size: 420 102 135 231 275 519 677 1148
Cuts at: 5113 5358 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 519 420 275 245 231
135 102
NarI GG'CG_CC
Cuts at: 2710 2710
Size: 5872
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5320
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5872
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5872
NotI GC'GGCC_GC
Cuts at: 2305 3319 2305
Size: 1014 4858
NspI r_CATG'y
Cuts at: 1132 1229 2787 3230 4775 1132
Size: 97 1558 443 1545 2229
Fragments arranged by size:
2229 1558 1545 443 97
PasI CC'CwG_GG
Cuts at: 1348 2984 1348
Size: 1636 4236
PciI A'CATG_T
Cuts at: 4771 4771
Size: 5872
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2611 1108
Size: 1503 4369
PmeI GTTT'AAAC
Cuts at: 3176 3176
Size: 5872
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3941 3973 5454 5486 3941
Size: 32 1481 32 4327
Fragments arranged by size:
4327 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5872
PsiI TTA'TAA
Cuts at: 3442 3442
Size: 5872
PstI C_TGCA'G
Cuts at: 830 2414 2493 2499 3224 830
Size: 1584 79 6 725 3478
Fragments arranged by size:
3478 1584 725 79 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5872
PvuII CAG'CTG
Cuts at: 2263 2494 2769 2263
Size: 231 275 5366
Fragments arranged by size:
5366 275 231
SacII CC_GC'GG
Cuts at: 2027 2027
Size: 5872
SalI G'TCGA_C
Cuts at: 1936 3214 1936
Size: 1278 4594
SbfI CC_TGCA'GG
Cuts at: 3224 3224
Size: 5872
ScaI AGT'ACT
Cuts at: 4120 5832 4120
Size: 1712 4160
SfcI C'TryA_G
Cuts at: 826 1045 2410 2489 2495 3220 4355 5036 5227
Size: 219 1365 79 6 725 1135 681 191
Cuts at: 5227 826
Size: 1471
Fragments arranged by size:
1471 1365 1135 725 681 219 191 79
6
SfoI GGC'GCC
Cuts at: 2711 2711
Size: 5872
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5872
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5872
SmaI CCC'GGG
Cuts at: 1872 2666 3199 1872
Size: 794 533 4545
Fragments arranged by size:
4545 794 533
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2294 2333 2554 3976 4877 5139
Size: 197 926 351 39 221 1422 901 262
Cuts at: 5139 820
Size: 1553
Fragments arranged by size:
1553 1422 926 901 351 262 221 197
39
SnaBI TAC'GTA
Cuts at: 493 3663 493
Size: 3170 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5872
SphI G_CATG'C
Cuts at: 1132 3230 1132
Size: 2098 3774
SspI AAT'ATT
Cuts at: 5 52 3796 5
Size: 47 3744 2081
Fragments arranged by size:
3744 2081 47
StuI AGG'CCT
Cuts at: 1517 2184 1517
Size: 667 5205
StyI C'CwwG_G
Cuts at: 513 1065 3311 513
Size: 552 2246 3074
Fragments arranged by size:
3074 2246 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3145 3899 3916 4069 4254 4699 325
Size: 1255 1565 754 17 153 185 445 1498
Fragments arranged by size:
1565 1498 1255 754 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4118 5830 96
Size: 274 80 33 51 1443 2141 1712 138
Fragments arranged by size:
2141 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2440 2472 5832 5864 2440
Size: 32 3360 32 2448
Fragments arranged by size:
3360 2448 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2155 2212 1316
Size: 839 57 4976
Fragments arranged by size:
4976 839 57
XbaI T'CTAG_A
Cuts at: 3208 3208
Size: 5872
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3022 3022
Size: 5872
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5872
XmaI C'CCGG_G
Cuts at: 1870 2664 3197 1870
Size: 794 533 4545
Fragments arranged by size:
4545 794 533
XmnI GAAnn'nnTTC
Cuts at: 4001 4001
Size: 5872
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3680 276
Size: 53 83 186 936 2146 2468
Fragments arranged by size:
2468 2146 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SanDI SapI SexAI SfiI SrfI SwaI