Restriction map for
pFN21AE1863
(Circular) (Six-base) MAPSORT of: pFN21AE1863.seq Check: 2428 from: 1 to: 5851
pFN21AE1863 5851 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2074 2150 2074
Size: 76 5775
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3661 278
Size: 53 83 186 936 2125 2468
Fragments arranged by size:
2468 2125 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3194 1937
Size: 1257 4594
Acc65I G'GTAC_C
Cuts at: 3172 3172
Size: 5851
AclI AA'CG_TT
Cuts at: 1563 3978 4351 1563
Size: 2415 373 3063
Fragments arranged by size:
3063 2415 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3914 5298 5566 1632
Size: 2282 1384 268 1917
Fragments arranged by size:
2282 1917 1384 268
AfeI AGC'GCT
Cuts at: 2567 2567
Size: 5851
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5654
AflIII A'CryG_T
Cuts at: 1203 4750 5727 1203
Size: 3547 977 1327
Fragments arranged by size:
3547 1327 977
AgeI A'CCGG_T
Cuts at: 4682 4682
Size: 5851
AhdI GACnn_n'nnGTC
Cuts at: 4580 4580
Size: 5851
AleI CACnn'nnGTG
Cuts at: 1166 2426 1166
Size: 1260 4591
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3002 1184
Size: 1818 4033
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2534 2566 2534
Size: 32 5819
AlwNI CAG_nnn'CTG
Cuts at: 2476 3594 5166 2476
Size: 1118 1572 3161
Fragments arranged by size:
3161 1572 1118
ApaI G_GGCC'C
Cuts at: 2501 2904 2501
Size: 403 5448
ApaLI G'TGCA_C
Cuts at: 3908 5064 3908
Size: 1156 4695
ApoI r'AATT_y
Cuts at: 1493 3160 3386 3538 5647 1493
Size: 1667 226 152 2109 1697
Fragments arranged by size:
2109 1697 1667 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4405 160
Size: 872 3373 1606
Fragments arranged by size:
3373 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5851
AvaI C'yCGr_G
Cuts at: 1870 1943 3176 1870
Size: 73 1233 4545
Fragments arranged by size:
4545 1233 73
BamHI G'GATC_C
Cuts at: 3181 3548 3181
Size: 367 5484
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2123 2380 3042 3172 4627
Size: 325 217 627 336 257 662 130 1455
Cuts at: 4627 618
Size: 1842
Fragments arranged by size:
1842 1455 662 627 336 325 257 217
130
BanII G_rGCy'C
Cuts at: 1391 2049 2094 2294 2501 2529 2649 2820 2837
Size: 658 45 200 207 28 120 171 17
Cuts at: 2837 2858 2904 3170 1391
Size: 21 46 266 4072
Fragments arranged by size:
4072 658 266 207 200 171 120 46
45 28 21 17
BbeI G_GCGC'C
Cuts at: 2127 2127
Size: 5851
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4880
BbvCI CC'TCA_GC
Cuts at: 3051 3051
Size: 5851
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2614 2648 4042 4076 1903
Size: 34 677 34 1394 34 3678
Fragments arranged by size:
3678 1394 677 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3743 4953 3743
Size: 1210 4641
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5851
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2408 4462 136
Size: 107 122 71 631 605 736 2054 1525
Fragments arranged by size:
2054 1525 736 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2886 5846 2886
Size: 2960 2891
BlpI GC'TnA_GC
Cuts at: 2648 3268 2648
Size: 620 5231
Bme1580I G_kGCm'C
Cuts at: 1790 2501 2904 3912 5068 1790
Size: 711 403 1008 1156 2573
Fragments arranged by size:
2573 1156 1008 711 403
BmgBI CAC'GTC
Cuts at: 1318 2545 1318
Size: 1227 4624
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3582 4540 442
Size: 416 53 83 399 24 2165 958 1753
Fragments arranged by size:
2165 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5851
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2051 2498 4511 1239
Size: 812 447 2013 2579
Fragments arranged by size:
2579 2013 812 447
Bpu10I CC'TnA_GC
Cuts at: 3051 3051
Size: 5851
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3976 4841 5139 3976
Size: 865 298 4688
Fragments arranged by size:
4688 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4514 882
Size: 617 3015 2219
Fragments arranged by size:
3015 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3642 493
Size: 711 2438 2702
Fragments arranged by size:
2702 2438 711
BsaBI GATnn'nnATC
Cuts at: 5845 5845
Size: 5851
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2124 3658 4040 4675
Size: 53 83 186 936 591 1534 382 635
Cuts at: 4675 275
Size: 1451
Fragments arranged by size:
1534 1451 936 635 591 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4284 4682 4956 5103 5514 4284
Size: 398 274 147 411 4621
Fragments arranged by size:
4621 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2007 2037 2429 2459 2696
Size: 30 165 30 604 30 392 30 237
Cuts at: 2696 2720 2726 2744 2750 2768 2774 2792 2798
Size: 24 6 18 6 18 6 18 6
Cuts at: 2798 2822 2985 3015 1178
Size: 24 163 30 4014
Fragments arranged by size:
4014 604 392 237 165 163 30 30
30 30 30 24 24 18 18 18
6 6 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 2042 2528 2531 2534 2537 1186
Size: 853 3 486 3 3 3 4500
Fragments arranged by size:
4500 853 486 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2368 2826 2897 2989 3110 5054 5550 2368
Size: 458 71 92 121 1944 496 2669
Fragments arranged by size:
2669 1944 496 458 121 92 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2198 2234 2345 2528 2660 1163
Size: 573 462 36 111 183 132 4354
Fragments arranged by size:
4354 573 462 183 132 111 36
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2271 3301 4062 5090 1540
Size: 275 186 270 1030 761 1028 2301
Fragments arranged by size:
2301 1030 1028 761 275 270 186
BsiHKAI G_wGCw'C
Cuts at: 2649 3912 3997 5068 2649
Size: 1263 85 1071 3432
Fragments arranged by size:
3432 1263 1071 85
BsmI GAATG_Cn'
Cuts at: 3362 3455 3362
Size: 93 5758
BsmBI CGTCTCn'nnnn_
Cuts at: 2368 2368
Size: 5851
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2094 2294 2501 2529 2649 2820
Size: 399 259 45 200 207 28 120 171
Cuts at: 2820 2837 2858 2904 3170 3912 3997 5068 1391
Size: 17 21 46 266 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 266 259 207 200
171 120 85 46 45 28 21 17
BspEI T'CCGG_A
Cuts at: 5514 5514
Size: 5851
BspHI T'CATG_A
Cuts at: 3740 5470 3740
Size: 1730 4121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2074 2150 2950 3206 844
Size: 1230 76 800 256 3489
Fragments arranged by size:
3489 1230 800 256 76
BsrBI CCG'CTC
Cuts at: 1394 1823 3738 1394
Size: 429 1915 3507
Fragments arranged by size:
3507 1915 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4346 4520 65
Size: 1183 3098 174 1396
Fragments arranged by size:
3098 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2101 3039 3125 4495 4682 1700
Size: 110 291 938 86 1370 187 2869
Fragments arranged by size:
2869 1370 938 291 187 110 86
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5851
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5851
BssSI C'ACGA_G
Cuts at: 3911 4923 3911
Size: 1012 4839
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2323 794
Size: 1529 4322
BstBI TT'CG_AA
Cuts at: 4655 4655
Size: 5851
BstEII G'GTnAC_C
Cuts at: 1184 2387 2948 1184
Size: 1203 561 4087
Fragments arranged by size:
4087 1203 561
BstYI r'GATC_y
Cuts at: 1972 2886 3181 3548 3933 3950 5402 5488 5500
Size: 914 295 367 385 17 1452 86 12
Cuts at: 5500 5846 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 914 385 367 346 295 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2112 2164 2427 2961 513
Size: 552 939 108 52 263 534 3403
Fragments arranged by size:
3403 939 552 534 263 108 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5112
Fragments arranged by size:
5112 683 56
BtsI GCAGTG_nn'
Cuts at: 2186 2986 3367 4161 4181 2186
Size: 800 381 794 20 3856
Fragments arranged by size:
3856 800 794 381 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5851
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5816
DraI TTT'AAA
Cuts at: 3155 3155
Size: 5851
DraIII CAC_nnn'GTG
Cuts at: 2323 2323
Size: 5851
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4858 809
Size: 507 3542 1802
Fragments arranged by size:
3542 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2056 2109 2268
Size: 54 1425 50 275 18 226 53 159
Cuts at: 2268 3127 3298 4187 5816 8
Size: 859 171 889 1629 43
Fragments arranged by size:
1629 1425 889 859 275 226 171 159
54 53 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2268 3298 1537
Size: 275 456 1030 4090
Fragments arranged by size:
4090 1030 456 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3781 1353
Size: 2428 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4438 4812 4958 387
Size: 1064 138 2849 374 146 1280
Fragments arranged by size:
2849 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2647 2647
Size: 5851
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2498 3914 4511 5298 5566 1239
Size: 393 419 447 1416 597 787 268 1524
Fragments arranged by size:
1524 1416 787 597 447 419 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3050 3050
Size: 5851
EcoO109I rG'GnC_Cy
Cuts at: 2169 2188 2274 2589 2169
Size: 19 86 315 5431
Fragments arranged by size:
5431 315 86 19
EcoRI G'AATT_C
Cuts at: 3160 3538 3160
Size: 378 5473
FspI TGC'GCA
Cuts at: 2249 4357 2249
Size: 2108 3743
HaeII r_GCGC'y
Cuts at: 1678 2127 2569 4998 1678
Size: 449 442 2429 2531
Fragments arranged by size:
2531 2429 449 442
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2007 2039 2696 2720 2728
Size: 32 135 32 435 32 657 24 8
Cuts at: 2728 2744 2752 2768 2776 2792 2800 2824 2904
Size: 16 8 16 8 16 8 24 80
Cuts at: 2904 2936 2985 3017 4492 4524 4566 4598 1373
Size: 32 49 32 1475 32 42 32 2626
Fragments arranged by size:
2626 1475 657 435 135 80 49 42
32 32 32 32 32 32 32 24
24 16 16 16 8 8 8 8
HincII GTy'rAC
Cuts at: 669 1657 1938 3195 3441 669
Size: 988 281 1257 246 3079
Fragments arranged by size:
3079 1257 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5851
HpaI GTT'AAC
Cuts at: 3441 3441
Size: 5851
KasI G'GCGC_C
Cuts at: 2123 2123
Size: 5851
KpnI G_GTAC'C
Cuts at: 3176 3176
Size: 5851
MfeI C'AATT_G
Cuts at: 3450 3450
Size: 5851
MluI A'CGCG_T
Cuts at: 5727 5727
Size: 5851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2199 4965 5149 1305
Size: 894 2766 184 2007
Fragments arranged by size:
2766 2007 894 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4029
Fragments arranged by size:
4029 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2426 3809 4168 4327 518
Size: 648 1260 1383 359 159 2042
Fragments arranged by size:
2042 1383 1260 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2166 2356 2956 2963 3267 3944 5092 5337
Size: 560 190 600 7 304 677 1148 245
Cuts at: 5337 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 600 560 304 245 190
7
NaeI GCC'GGC
Cuts at: 3041 3041
Size: 5851
NarI GG'CG_CC
Cuts at: 2124 2124
Size: 5851
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4360
Fragments arranged by size:
4360 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5851
NgoMIV G'CCGG_C
Cuts at: 3039 3039
Size: 5851
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5851
NotI GC'GGCC_GC
Cuts at: 3298 3298
Size: 5851
NspI r_CATG'y
Cuts at: 1132 1229 3209 4754 1132
Size: 97 1980 1545 2229
Fragments arranged by size:
2229 1980 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2494 2530 2584 1348
Size: 1146 36 54 4615
Fragments arranged by size:
4615 1146 54 36
PciI A'CATG_T
Cuts at: 4750 4750
Size: 5851
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2476 1108
Size: 1368 4483
PfoI T'CCnGG_A
Cuts at: 2346 2346
Size: 5851
PmeI GTTT'AAAC
Cuts at: 3155 3155
Size: 5851
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3920 3952 5433 5465 3920
Size: 32 1481 32 4306
Fragments arranged by size:
4306 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2274 2589 2274
Size: 315 5536
PshAI GACnn'nnGTC
Cuts at: 1613 2594 1613
Size: 981 4870
PsiI TTA'TAA
Cuts at: 3421 3421
Size: 5851
PspOMI G'GGCC_C
Cuts at: 2497 2900 2497
Size: 403 5448
PstI C_TGCA'G
Cuts at: 830 2085 3203 830
Size: 1255 1118 3478
Fragments arranged by size:
3478 1255 1118
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5851
PvuII CAG'CTG
Cuts at: 2356 2956 2356
Size: 600 5251
RsrII CG'GwC_CG
Cuts at: 2161 2161
Size: 5851
SacI G_AGCT'C
Cuts at: 2649 2649
Size: 5851
SacII CC_GC'GG
Cuts at: 2167 2964 2167
Size: 797 5054
SalI G'TCGA_C
Cuts at: 1936 3193 1936
Size: 1257 4594
SanDI GG'GwC_CC
Cuts at: 2274 2589 2274
Size: 315 5536
SbfI CC_TGCA'GG
Cuts at: 2085 3203 2085
Size: 1118 4733
ScaI AGT'ACT
Cuts at: 4099 5811 4099
Size: 1712 4139
SfcI C'TryA_G
Cuts at: 826 1045 2081 3199 4334 5015 5206 826
Size: 219 1036 1118 1135 681 191 1471
Fragments arranged by size:
1471 1135 1118 1036 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2408 2408
Size: 5851
SfoI GGC'GCC
Cuts at: 2125 2125
Size: 5851
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5851
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5851
SmaI CCC'GGG
Cuts at: 1872 3178 1872
Size: 1306 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3955 4856 5118 820
Size: 197 926 2012 901 262 1553
Fragments arranged by size:
2012 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3642 493
Size: 3149 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5851
SphI G_CATG'C
Cuts at: 1132 3209 1132
Size: 2077 3774
SspI AAT'ATT
Cuts at: 5 52 3775 5
Size: 47 3723 2081
Fragments arranged by size:
3723 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5851
StyI C'CwwG_G
Cuts at: 513 1065 2004 2118 3290 513
Size: 552 939 114 1172 3074
Fragments arranged by size:
3074 1172 939 552 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3077 3081 3878 3895 4048 4233 4678
Size: 1255 1497 4 797 17 153 185 445
Cuts at: 4678 325
Size: 1498
Fragments arranged by size:
1498 1497 1255 797 445 185 153 17
4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4097 5809 96
Size: 274 80 33 51 1443 2120 1712 138
Fragments arranged by size:
2120 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2965 2997 5811 5843 2965
Size: 32 2814 32 2973
Fragments arranged by size:
2973 2814 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5851
XbaI T'CTAG_A
Cuts at: 3187 3187
Size: 5851
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5851
XmaI C'CCGG_G
Cuts at: 1870 3176 1870
Size: 1306 4545
XmnI GAAnn'nnTTC
Cuts at: 3980 3980
Size: 5851
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3659 276
Size: 53 83 186 936 2125 2468
Fragments arranged by size:
2468 2125 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I
Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI
PmlI PspXI PsrI SapI SexAI SrfI SwaI XcmI