(Circular) (Six-base) MAPSORT of: pFN21AE1861.seq Check: 4272 from: 1 to: 5845 pFN21AE1861 5845 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2495 2495 Size: 5845 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2990 3655 278 Size: 53 83 186 936 1454 665 2468 Fragments arranged by size: 2468 1454 936 665 186 83 53 AccI GT'mk_AC Cuts at: 1937 2904 3188 1937 Size: 967 284 4594 Fragments arranged by size: 4594 967 284 Acc65I G'GTAC_C Cuts at: 3166 3166 Size: 5845 AclI AA'CG_TT Cuts at: 1563 3972 4345 1563 Size: 2409 373 3063 Fragments arranged by size: 3063 2409 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2771 2822 3908 5292 5560 1632 Size: 1139 51 1086 1384 268 1917 Fragments arranged by size: 1917 1384 1139 1086 268 51 AfeI AGC'GCT Cuts at: 3065 3065 Size: 5845 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5648 AflIII A'CryG_T Cuts at: 1203 4744 5721 1203 Size: 3541 977 1327 Fragments arranged by size: 3541 1327 977 AgeI A'CCGG_T Cuts at: 4676 4676 Size: 5845 AhdI GACnn_n'nnGTC Cuts at: 4574 4574 Size: 5845 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5845 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2402 1184 Size: 1218 4627 AlwNI CAG_nnn'CTG Cuts at: 3588 5160 3588 Size: 1572 4273 ApaLI G'TGCA_C Cuts at: 3112 3902 5058 3112 Size: 790 1156 3899 Fragments arranged by size: 3899 1156 790 ApoI r'AATT_y Cuts at: 1493 3154 3380 3532 5641 1493 Size: 1661 226 152 2109 1697 Fragments arranged by size: 2109 1697 1661 226 152 AseI AT'TA_AT Cuts at: 160 1032 4399 160 Size: 872 3367 1606 Fragments arranged by size: 3367 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 AvaI C'yCGr_G Cuts at: 1870 1943 3170 1870 Size: 73 1227 4545 Fragments arranged by size: 4545 1227 73 BamHI G'GATC_C Cuts at: 3175 3542 3175 Size: 367 5478 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3166 4621 618 Size: 325 217 627 1379 1455 1842 Fragments arranged by size: 1842 1455 1379 627 325 217 BanII G_rGCy'C Cuts at: 1391 2633 3015 3164 1391 Size: 1242 382 149 4072 Fragments arranged by size: 4072 1242 382 149 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2061 2303 2759 928 Size: 971 162 242 456 4014 Fragments arranged by size: 4014 971 456 242 162 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3103 3137 4036 4070 1903 Size: 34 1166 34 899 34 3678 Fragments arranged by size: 3678 1166 899 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3737 4947 3737 Size: 1210 4635 BclI T'GATC_A Cuts at: 1548 1548 Size: 5845 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2472 4456 136 Size: 107 122 71 631 605 800 1984 1525 Fragments arranged by size: 1984 1525 800 631 605 122 107 71 BglII A'GATC_T Cuts at: 5840 5840 Size: 5845 BlpI GC'TnA_GC Cuts at: 3262 3262 Size: 5845 Bme1580I G_kGCm'C Cuts at: 1790 2963 3116 3906 5062 1790 Size: 1173 153 790 1156 2573 Fragments arranged by size: 2573 1173 1156 790 153 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5845 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3576 4534 442 Size: 416 53 83 399 24 2159 958 1753 Fragments arranged by size: 2159 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5845 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4505 1239 Size: 3266 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2217 3970 4835 5133 2217 Size: 1753 865 298 2929 Fragments arranged by size: 2929 1753 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4508 882 Size: 617 3009 2219 Fragments arranged by size: 3009 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3636 493 Size: 711 2432 2702 Fragments arranged by size: 2702 2432 711 BsaBI GATnn'nnATC Cuts at: 5839 5839 Size: 5845 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2598 2987 3652 4034 Size: 53 83 186 936 1065 389 665 382 Cuts at: 4034 4669 275 Size: 635 1451 Fragments arranged by size: 1451 1065 936 665 635 389 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4278 4676 4950 5097 5508 4278 Size: 398 274 147 411 4615 Fragments arranged by size: 4615 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5620 Fragments arranged by size: 5620 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5845 BseYI C'CCAG_C Cuts at: 2915 2956 5048 5544 2915 Size: 41 2092 496 3216 Fragments arranged by size: 3216 2092 496 41 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2648 3074 1163 Size: 573 912 426 3934 Fragments arranged by size: 3934 912 573 426 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2683 3028 3295 4056 5084 1540 Size: 275 186 682 345 267 761 1028 2301 Fragments arranged by size: 2301 1028 761 682 345 275 267 186 BsiHKAI G_wGCw'C Cuts at: 2200 2647 2948 3015 3116 3906 3991 5062 2200 Size: 447 301 67 101 790 85 1071 2983 Fragments arranged by size: 2983 1071 790 447 301 101 85 67 BsmI GAATG_Cn' Cuts at: 2318 3356 3449 2318 Size: 1038 93 4714 Fragments arranged by size: 4714 1038 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2399 2399 Size: 5845 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2200 2633 2647 2948 2963 3015 3116 Size: 399 410 433 14 301 15 52 101 Cuts at: 3116 3164 3906 3991 5062 1391 Size: 48 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 433 410 399 301 101 85 52 48 15 14 BspEI T'CCGG_A Cuts at: 5508 5508 Size: 5845 BspHI T'CATG_A Cuts at: 2655 3734 5464 2655 Size: 1079 1730 3036 Fragments arranged by size: 3036 1730 1079 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2495 2529 2670 3200 844 Size: 1651 34 141 530 3489 Fragments arranged by size: 3489 1651 530 141 34 BsrBI CCG'CTC Cuts at: 1394 1823 2608 3732 1394 Size: 429 785 1124 3507 Fragments arranged by size: 3507 1124 785 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2070 2944 4340 4514 65 Size: 1183 822 874 1396 174 1396 Fragments arranged by size: 1396 1396 1183 874 822 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2678 2948 4489 4676 1700 Size: 110 868 270 1541 187 2869 Fragments arranged by size: 2869 1541 868 270 187 110 BsrGI T'GTAC_A Cuts at: 96 2694 96 Size: 2598 3247 BssHII G'CGCG_C Cuts at: 1925 2507 1925 Size: 582 5263 BssSI C'ACGA_G Cuts at: 3905 4917 3905 Size: 1012 4833 BstAPI GCAn_nnn'nTGC Cuts at: 794 3111 794 Size: 2317 3528 BstBI TT'CG_AA Cuts at: 4649 4649 Size: 5845 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5845 BstYI r'GATC_y Cuts at: 1972 3175 3542 3927 3944 5396 5482 5494 5840 Size: 1203 367 385 17 1452 86 12 346 Cuts at: 5840 1972 Size: 1977 Fragments arranged by size: 1977 1452 1203 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2525 2725 3028 513 Size: 552 1460 200 303 3330 Fragments arranged by size: 3330 1460 552 303 200 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5106 Fragments arranged by size: 5106 683 56 BtsI GCAGTG_nn' Cuts at: 3070 3361 4155 4175 3070 Size: 291 794 20 4740 Fragments arranged by size: 4740 794 291 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5845 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5810 DraI TTT'AAA Cuts at: 3149 3149 Size: 5845 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4852 809 Size: 507 3536 1802 Fragments arranged by size: 3536 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2094 2680 2826 Size: 54 1425 50 275 18 264 586 146 Cuts at: 2826 3025 3292 4181 5810 8 Size: 199 267 889 1629 43 Fragments arranged by size: 1629 1425 889 586 275 267 264 199 146 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2680 3025 3292 1537 Size: 275 868 345 267 4090 Fragments arranged by size: 4090 868 345 275 267 EarI CTCTTCn'nnn_ Cuts at: 1353 3775 1353 Size: 2422 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4432 4806 4952 387 Size: 1064 138 2843 374 146 1280 Fragments arranged by size: 2843 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3013 3013 Size: 5845 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2771 2822 3908 4505 5292 5560 1239 Size: 393 1139 51 1086 597 787 268 1524 Fragments arranged by size: 1524 1139 1086 787 597 393 268 51 EcoO109I rG'GnC_Cy Cuts at: 2518 2518 Size: 5845 EcoRI G'AATT_C Cuts at: 3154 3532 3154 Size: 378 5467 FseI GG_CCGG'CC Cuts at: 2682 2682 Size: 5845 FspI TGC'GCA Cuts at: 2936 4351 2936 Size: 1415 4430 HaeII r_GCGC'y Cuts at: 1678 3067 4992 1678 Size: 1389 1925 2531 Fragments arranged by size: 2531 1925 1389 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2351 2383 4486 4518 4560 Size: 32 135 32 779 32 2103 32 42 Cuts at: 4560 4592 1373 Size: 32 2626 Fragments arranged by size: 2626 2103 779 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2866 3189 3435 669 Size: 988 281 928 323 246 3079 Fragments arranged by size: 3079 988 928 323 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5845 HpaI GTT'AAC Cuts at: 3435 3435 Size: 5845 KpnI G_GTAC'C Cuts at: 3170 3170 Size: 5845 MfeI C'AATT_G Cuts at: 3444 3444 Size: 5845 MluI A'CGCG_T Cuts at: 5721 5721 Size: 5845 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2353 2970 4959 5143 1305 Size: 1048 617 1989 184 2007 Fragments arranged by size: 2007 1989 1048 617 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2096 10 Size: 54 1425 343 264 3759 Fragments arranged by size: 3759 1425 343 264 54 MslI CAynn'nnrTG Cuts at: 518 1166 2654 3803 4162 4321 518 Size: 648 1488 1149 359 159 2042 Fragments arranged by size: 2042 1488 1149 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2527 2770 3261 3938 5086 5331 1606 Size: 921 243 491 677 1148 245 2120 Fragments arranged by size: 2120 1148 921 677 491 245 243 NaeI GCC'GGC Cuts at: 2680 2680 Size: 5845 NcoI C'CATG_G Cuts at: 513 1065 2725 513 Size: 552 1660 3633 Fragments arranged by size: 3633 1660 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5845 NgoMIV G'CCGG_C Cuts at: 2678 2678 Size: 5845 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5845 NotI GC'GGCC_GC Cuts at: 3292 3292 Size: 5845 NspI r_CATG'y Cuts at: 1132 1229 2275 2760 3203 4748 1132 Size: 97 1046 485 443 1545 2229 Fragments arranged by size: 2229 1545 1046 485 443 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5845 PciI A'CATG_T Cuts at: 4744 4744 Size: 5845 PflMI CCAn_nnn'nTGG Cuts at: 1108 2139 1108 Size: 1031 4814 PmeI GTTT'AAAC Cuts at: 3149 3149 Size: 5845 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3914 3946 5427 5459 3914 Size: 32 1481 32 4300 Fragments arranged by size: 4300 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2518 2518 Size: 5845 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5845 PsiI TTA'TAA Cuts at: 3415 3415 Size: 5845 PstI C_TGCA'G Cuts at: 830 2213 2351 2498 2769 3197 830 Size: 1383 138 147 271 428 3478 Fragments arranged by size: 3478 1383 428 271 147 138 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5845 PvuII CAG'CTG Cuts at: 2770 2770 Size: 5845 SacI G_AGCT'C Cuts at: 3015 3015 Size: 5845 SacII CC_GC'GG Cuts at: 2528 2528 Size: 5845 SalI G'TCGA_C Cuts at: 1936 3187 1936 Size: 1251 4594 SbfI CC_TGCA'GG Cuts at: 3197 3197 Size: 5845 ScaI AGT'ACT Cuts at: 4093 5805 4093 Size: 1712 4133 SexAI A'CCwGG_T Cuts at: 2898 2898 Size: 5845 SfcI C'TryA_G Cuts at: 826 1045 2209 2347 2494 2765 3193 4328 5009 Size: 219 1164 138 147 271 428 1135 681 Cuts at: 5009 5200 826 Size: 191 1471 Fragments arranged by size: 1471 1164 1135 681 428 271 219 191 147 138 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5845 SmaI CCC'GGG Cuts at: 1872 3172 1872 Size: 1300 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2232 3949 4850 5112 820 Size: 197 926 289 1717 901 262 1553 Fragments arranged by size: 1717 1553 926 901 289 262 197 SnaBI TAC'GTA Cuts at: 493 3636 493 Size: 3143 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5845 SphI G_CATG'C Cuts at: 1132 3203 1132 Size: 2071 3774 SspI AAT'ATT Cuts at: 5 52 2025 3769 5 Size: 47 1973 1744 2081 Fragments arranged by size: 2081 1973 1744 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5845 StyI C'CwwG_G Cuts at: 513 1065 2514 2583 2625 2725 3284 513 Size: 552 1449 69 42 100 559 3074 Fragments arranged by size: 3074 1449 559 552 100 69 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2780 3872 3889 4042 4227 4672 325 Size: 1255 1200 1092 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1200 1092 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2277 2694 4091 Size: 274 80 33 51 1443 300 417 1397 Cuts at: 4091 5803 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1397 417 300 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5805 5837 5805 Size: 32 5813 Tth111I GACn'n_nGTC Cuts at: 1316 2900 1316 Size: 1584 4261 XbaI T'CTAG_A Cuts at: 3181 3181 Size: 5845 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2590 2896 2590 Size: 306 5539 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5845 XmaI C'CCGG_G Cuts at: 1870 3170 1870 Size: 1300 4545 XmnI GAAnn'nnTTC Cuts at: 3974 3974 Size: 5845 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2988 3653 276 Size: 53 83 186 936 1454 665 2468 Fragments arranged by size: 2468 1454 936 665 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SanDI SapI SfiI SfoI SrfI SwaI