Restriction map for
pFN21AE1859
(Circular) (Six-base) MAPSORT of: pFN21AE1859.seq Check: 5441 from: 1 to: 5842
pFN21AE1859 5842 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3652 278
Size: 53 83 186 936 2116 2468
Fragments arranged by size:
2468 2116 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3185 1937
Size: 1248 4594
Acc65I G'GTAC_C
Cuts at: 3163 3163
Size: 5842
AclI AA'CG_TT
Cuts at: 1563 3969 4342 1563
Size: 2406 373 3063
Fragments arranged by size:
3063 2406 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2420 3905 5289 5557 1632
Size: 788 1485 1384 268 1917
Fragments arranged by size:
1917 1485 1384 788 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5645
AflIII A'CryG_T
Cuts at: 1203 4741 5718 1203
Size: 3538 977 1327
Fragments arranged by size:
3538 1327 977
AgeI A'CCGG_T
Cuts at: 4673 4673
Size: 5842
AhdI GACnn_n'nnGTC
Cuts at: 4571 4571
Size: 5842
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5842
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5842
AlwNI CAG_nnn'CTG
Cuts at: 3585 5157 3585
Size: 1572 4270
ApaLI G'TGCA_C
Cuts at: 3899 5055 3899
Size: 1156 4686
ApoI r'AATT_y
Cuts at: 1493 2288 2564 2639 3151 3377 3529 5638 1493
Size: 795 276 75 512 226 152 2109 1697
Fragments arranged by size:
2109 1697 795 512 276 226 152 75
AseI AT'TA_AT
Cuts at: 160 1032 4396 160
Size: 872 3364 1606
Fragments arranged by size:
3364 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5842
AvaI C'yCGr_G
Cuts at: 1870 1943 2051 2364 3167 1870
Size: 73 108 313 803 4545
Fragments arranged by size:
4545 803 313 108 73
BamHI G'GATC_C
Cuts at: 3172 3539 3172
Size: 367 5475
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2236 3163 4618 618
Size: 325 217 627 449 927 1455 1842
Fragments arranged by size:
1842 1455 927 627 449 325 217
BanII G_rGCy'C
Cuts at: 1391 2052 3161 1391
Size: 661 1109 4072
Fragments arranged by size:
4072 1109 661
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2970 3003 928
Size: 971 1071 33 3767
Fragments arranged by size:
3767 1071 971 33
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4033 4067 1903
Size: 34 2096 34 3678
Fragments arranged by size:
3678 2096 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3734 4944 3734
Size: 1210 4632
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5842
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4453 136
Size: 107 122 71 631 605 337 2444 1525
Fragments arranged by size:
2444 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5837 5837
Size: 5842
BlpI GC'TnA_GC
Cuts at: 3259 3259
Size: 5842
Bme1580I G_kGCm'C
Cuts at: 1790 3903 5059 1790
Size: 2113 1156 2573
Fragments arranged by size:
2573 2113 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5842
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3573 4531 442
Size: 416 53 83 399 24 2156 958 1753
Fragments arranged by size:
2156 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4502 1239
Size: 3263 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3967 4832 5130 3967
Size: 865 298 4679
Fragments arranged by size:
4679 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4505 882
Size: 617 3006 2219
Fragments arranged by size:
3006 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3633 493
Size: 711 2429 2702
Fragments arranged by size:
2702 2429 711
BsaBI GATnn'nnATC
Cuts at: 2229 5836 2229
Size: 3607 2235
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2147 3649 4031 4666
Size: 53 83 186 936 614 1502 382 635
Cuts at: 4666 275
Size: 1451
Fragments arranged by size:
1502 1451 936 635 614 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2233 2412 4275 4673 4947 5094 5505 2233
Size: 179 1863 398 274 147 411 2570
Fragments arranged by size:
2570 1863 411 398 274 179 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2171 2201 1178
Size: 30 165 30 768 30 4819
Fragments arranged by size:
4819 768 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2498 1186
Size: 1312 4530
BseYI C'CCAG_C
Cuts at: 2076 2097 2132 5045 5541 2076
Size: 21 35 2913 496 2377
Fragments arranged by size:
2913 2377 496 35 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5269
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2127 3292 4053 5081 1540
Size: 275 186 126 1165 761 1028 2301
Fragments arranged by size:
2301 1165 1028 761 275 186 126
BsiHKAI G_wGCw'C
Cuts at: 3903 3988 5059 3903
Size: 85 1071 4686
Fragments arranged by size:
4686 1071 85
BsmI GAATG_Cn'
Cuts at: 3353 3446 3353
Size: 93 5749
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 3161 3903 3988 5059 1391
Size: 399 262 1109 742 85 1071 2174
Fragments arranged by size:
2174 1109 1071 742 399 262 85
BspEI T'CCGG_A
Cuts at: 2412 5505 2412
Size: 3093 2749
BspHI T'CATG_A
Cuts at: 3731 5461 3731
Size: 1730 4112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3197 844
Size: 2353 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3729 1394
Size: 429 1906 3507
Fragments arranged by size:
3507 1906 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4337 4511 65
Size: 1183 3089 174 1396
Fragments arranged by size:
3089 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2059 4486 4673 1700
Size: 110 249 2427 187 2869
Fragments arranged by size:
2869 2427 249 187 110
BsrGI T'GTAC_A
Cuts at: 96 2199 3133 96
Size: 2103 934 2805
Fragments arranged by size:
2805 2103 934
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5842
BssSI C'ACGA_G
Cuts at: 3902 4914 3902
Size: 1012 4830
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5842
BstBI TT'CG_AA
Cuts at: 4646 4646
Size: 5842
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5842
BstXI CCAn_nnnn'nTGG
Cuts at: 2263 2263
Size: 5842
BstYI r'GATC_y
Cuts at: 1972 2230 2443 2545 3086 3172 3539 3924 3941
Size: 258 213 102 541 86 367 385 17
Cuts at: 3941 5393 5479 5491 5837 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 541 385 367 346 258 213
102 86 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2142 2154 2262 513
Size: 552 939 138 12 108 4093
Fragments arranged by size:
4093 939 552 138 108 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5103
Fragments arranged by size:
5103 683 56
BtsI GCAGTG_nn'
Cuts at: 2469 3358 4152 4172 2469
Size: 889 794 20 4139
Fragments arranged by size:
4139 889 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5842
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5807
DraI TTT'AAA
Cuts at: 2644 3146 2644
Size: 502 5340
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4849 809
Size: 507 3533 1802
Fragments arranged by size:
3533 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2124 3289
Size: 54 1425 50 275 18 180 114 1165
Cuts at: 3289 4178 5807 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1165 889 275 180 114 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2124 3289 1537
Size: 275 312 1165 4090
Fragments arranged by size:
4090 1165 312 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2109 3772 1353
Size: 756 1663 3423
Fragments arranged by size:
3423 1663 756
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4429 4803 4949 387
Size: 1064 138 2840 374 146 1280
Fragments arranged by size:
2840 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2420 3905 4502 5289 5557 1239
Size: 393 788 1485 597 787 268 1524
Fragments arranged by size:
1524 1485 788 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3151 3529 3151
Size: 378 5464
EcoRV GAT'ATC
Cuts at: 2956 2956
Size: 5842
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3020 3020
Size: 5842
FspI TGC'GCA
Cuts at: 2037 4348 2037
Size: 2311 3531
HaeII r_GCGC'y
Cuts at: 1678 4989 1678
Size: 3311 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2171 2203 4483 4515 4557
Size: 32 135 32 599 32 2280 32 42
Cuts at: 4557 4589 1373
Size: 32 2626
Fragments arranged by size:
2626 2280 599 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3186 3432 669
Size: 988 281 1248 246 3079
Fragments arranged by size:
3079 1248 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5842
HpaI GTT'AAC
Cuts at: 3432 3432
Size: 5842
KpnI G_GTAC'C
Cuts at: 3167 3167
Size: 5842
MfeI C'AATT_G
Cuts at: 3441 3441
Size: 5842
MluI A'CGCG_T
Cuts at: 5718 5718
Size: 5842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4956 5140 1305
Size: 3651 184 2007
Fragments arranged by size:
3651 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4020
Fragments arranged by size:
4020 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3800 4159 4318 518
Size: 648 2634 359 159 2042
Fragments arranged by size:
2634 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3258 3935 5083 5328 1606
Size: 1652 677 1148 245 2120
Fragments arranged by size:
2120 1652 1148 677 245
NaeI GCC'GGC
Cuts at: 2061 2061
Size: 5842
NcoI C'CATG_G
Cuts at: 513 1065 2004 2154 513
Size: 552 939 150 4201
Fragments arranged by size:
4201 939 552 150
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5842
NgoMIV G'CCGG_C
Cuts at: 2059 2059
Size: 5842
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5842
NotI GC'GGCC_GC
Cuts at: 3289 3289
Size: 5842
NspI r_CATG'y
Cuts at: 1132 1229 2971 3200 4745 1132
Size: 97 1742 229 1545 2229
Fragments arranged by size:
2229 1742 1545 229 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5842
PciI A'CATG_T
Cuts at: 4741 4741
Size: 5842
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5842
PmeI GTTT'AAAC
Cuts at: 3146 3146
Size: 5842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3911 3943 5424 5456 3911
Size: 32 1481 32 4297
Fragments arranged by size:
4297 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5842
PsiI TTA'TAA
Cuts at: 3412 3412
Size: 5842
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2708 2740 2708
Size: 32 5810
PstI C_TGCA'G
Cuts at: 830 2328 2667 3194 830
Size: 1498 339 527 3478
Fragments arranged by size:
3478 1498 527 339
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5842
SalI G'TCGA_C
Cuts at: 1936 3184 1936
Size: 1248 4594
SbfI CC_TGCA'GG
Cuts at: 3194 3194
Size: 5842
ScaI AGT'ACT
Cuts at: 4090 5802 4090
Size: 1712 4130
SfcI C'TryA_G
Cuts at: 826 1045 2324 2663 3190 4325 5006 5197 826
Size: 219 1279 339 527 1135 681 191 1471
Fragments arranged by size:
1471 1279 1135 681 527 339 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5842
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5842
SmaI CCC'GGG
Cuts at: 1872 2053 2366 3169 1872
Size: 181 313 803 4545
Fragments arranged by size:
4545 803 313 181
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3946 4847 5109 820
Size: 197 926 2003 901 262 1553
Fragments arranged by size:
2003 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3633 493
Size: 3140 2702
SpeI A'CTAG_T
Cuts at: 152 2809 152
Size: 2657 3185
SphI G_CATG'C
Cuts at: 1132 3200 1132
Size: 2068 3774
SrfI GCCC'GGGC
Cuts at: 2053 2053
Size: 5842
SspI AAT'ATT
Cuts at: 5 52 3068 3766 5
Size: 47 3016 698 2081
Fragments arranged by size:
3016 2081 698 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5842
StyI C'CwwG_G
Cuts at: 513 1065 2004 2154 3281 513
Size: 552 939 150 1127 3074
Fragments arranged by size:
3074 1127 939 552 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2100 3869 3886 4039 4224 4669 325
Size: 1255 520 1769 17 153 185 445 1498
Fragments arranged by size:
1769 1498 1255 520 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2199 3133 4088
Size: 274 80 33 51 1443 222 934 955
Cuts at: 4088 5800 96
Size: 1712 138
Fragments arranged by size:
1712 1443 955 934 274 222 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5802 5834 5802
Size: 32 5810
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5842
XbaI T'CTAG_A
Cuts at: 3178 3178
Size: 5842
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5842
XmaI C'CCGG_G
Cuts at: 1870 2051 2364 3167 1870
Size: 181 313 803 4545
Fragments arranged by size:
4545 803 313 181
XmnI GAAnn'nnTTC
Cuts at: 3000 3971 3000
Size: 971 4871
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3650 276
Size: 53 83 186 936 2116 2468
Fragments arranged by size:
2468 2116 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SwaI XcmI