Restriction map for
pFN21AE1858
(Circular) (Six-base) MAPSORT of: pFN21AE1858.seq Check: 8266 from: 1 to: 5842
pFN21AE1858 5842 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3652 278
Size: 53 83 186 936 2116 2468
Fragments arranged by size:
2468 2116 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3185 1937
Size: 1248 4594
Acc65I G'GTAC_C
Cuts at: 3163 3163
Size: 5842
AclI AA'CG_TT
Cuts at: 1563 3969 4342 1563
Size: 2406 373 3063
Fragments arranged by size:
3063 2406 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2086 2400 2753 3905 5289 5557 1632
Size: 454 314 353 1152 1384 268 1917
Fragments arranged by size:
1917 1384 1152 454 353 314 268
AflII C'TTAA_G
Cuts at: 820 1017 2639 820
Size: 197 1622 4023
Fragments arranged by size:
4023 1622 197
AflIII A'CryG_T
Cuts at: 1203 4741 5718 1203
Size: 3538 977 1327
Fragments arranged by size:
3538 1327 977
AgeI A'CCGG_T
Cuts at: 4673 4673
Size: 5842
AhdI GACnn_n'nnGTC
Cuts at: 4571 4571
Size: 5842
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5842
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3049 3059 1184
Size: 1865 10 3967
Fragments arranged by size:
3967 1865 10
AlwNI CAG_nnn'CTG
Cuts at: 2221 3585 5157 2221
Size: 1364 1572 2906
Fragments arranged by size:
2906 1572 1364
ApaLI G'TGCA_C
Cuts at: 3899 5055 3899
Size: 1156 4686
ApoI r'AATT_y
Cuts at: 1493 2433 2525 2612 3055 3151 3377 3529 5638
Size: 940 92 87 443 96 226 152 2109
Cuts at: 5638 1493
Size: 1697
Fragments arranged by size:
2109 1697 940 443 226 152 96 92
87
AseI AT'TA_AT
Cuts at: 160 1032 4396 160
Size: 872 3364 1606
Fragments arranged by size:
3364 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5842
AvaI C'yCGr_G
Cuts at: 1870 1943 3167 1870
Size: 73 1224 4545
Fragments arranged by size:
4545 1224 73
AvrII C'CTAG_G
Cuts at: 2875 2875
Size: 5842
BamHI G'GATC_C
Cuts at: 3172 3539 3172
Size: 367 5475
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3163 4618 618
Size: 325 217 627 1376 1455 1842
Fragments arranged by size:
1842 1455 1376 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2021 2682 3161 1391
Size: 630 661 479 4072
Fragments arranged by size:
4072 661 630 479
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2399 928
Size: 971 500 4371
Fragments arranged by size:
4371 971 500
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4033 4067 1903
Size: 34 2096 34 3678
Fragments arranged by size:
3678 2096 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3734 4944 3734
Size: 1210 4632
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5842
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4453 136
Size: 107 122 71 631 605 2781 1525
Fragments arranged by size:
2781 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5837 5837
Size: 5842
BlpI GC'TnA_GC
Cuts at: 2015 2020 2681 3259 2015
Size: 5 661 578 4598
Fragments arranged by size:
4598 661 578 5
Bme1580I G_kGCm'C
Cuts at: 1790 3903 5059 1790
Size: 2113 1156 2573
Fragments arranged by size:
2573 2113 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5842
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3573 4531 442
Size: 416 53 83 399 24 2156 958 1753
Fragments arranged by size:
2156 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5842
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2868 2868
Size: 5842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4502 1239
Size: 3263 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3967 4832 5130 3967
Size: 865 298 4679
Fragments arranged by size:
4679 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4505 882
Size: 617 3006 2219
Fragments arranged by size:
3006 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3633 493
Size: 711 2429 2702
Fragments arranged by size:
2702 2429 711
BsaBI GATnn'nnATC
Cuts at: 5836 5836
Size: 5842
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3649 4031 4666 275
Size: 53 83 186 936 2116 382 635 1451
Fragments arranged by size:
2116 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2437 4275 4673 4947 5094 5505 2437
Size: 1838 398 274 147 411 2774
Fragments arranged by size:
2774 1838 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5617
Fragments arranged by size:
5617 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2690 1186
Size: 1504 4338
BseYI C'CCAG_C
Cuts at: 5045 5541 5045
Size: 496 5346
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2903 1163
Size: 573 1167 4102
Fragments arranged by size:
4102 1167 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3292 4053 5081 1540
Size: 275 186 1291 761 1028 2301
Fragments arranged by size:
2301 1291 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2021 2682 3903 3988 5059 2021
Size: 661 1221 85 1071 2804
Fragments arranged by size:
2804 1221 1071 661 85
BsmI GAATG_Cn'
Cuts at: 2230 2554 3353 3446 2230
Size: 324 799 93 4626
Fragments arranged by size:
4626 799 324 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2682 3161 3903 3988 5059 1391
Size: 399 231 661 479 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 661 479 399 231 85
BspEI T'CCGG_A
Cuts at: 2437 5505 2437
Size: 3068 2774
BspHI T'CATG_A
Cuts at: 2529 3731 5461 2529
Size: 1202 1730 2910
Fragments arranged by size:
2910 1730 1202
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3197 844
Size: 2353 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3729 1394
Size: 429 1906 3507
Fragments arranged by size:
3507 1906 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4337 4511 65
Size: 1183 3089 174 1396
Fragments arranged by size:
3089 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4486 4673 1700
Size: 110 2676 187 2869
Fragments arranged by size:
2869 2676 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5842
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5842
BssSI C'ACGA_G
Cuts at: 3902 4914 3902
Size: 1012 4830
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5842
BstBI TT'CG_AA
Cuts at: 4646 4646
Size: 5842
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5842
BstYI r'GATC_y
Cuts at: 1972 2887 3172 3539 3924 3941 5393 5479 5491
Size: 915 285 367 385 17 1452 86 12
Cuts at: 5491 5837 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 915 385 367 346 285 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3103 3103
Size: 5842
BtgI C'CryG_G
Cuts at: 513 1065 2004 2124 2724 513
Size: 552 939 120 600 3631
Fragments arranged by size:
3631 939 600 552 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3007 487
Size: 683 56 1781 3322
Fragments arranged by size:
3322 1781 683 56
BtsI GCAGTG_nn'
Cuts at: 3060 3358 4152 4172 3060
Size: 298 794 20 4730
Fragments arranged by size:
4730 794 298 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5842
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2224 2259 529
Size: 35 1660 35 4112
Fragments arranged by size:
4112 1660 35 35
DraI TTT'AAA
Cuts at: 3146 3146
Size: 5842
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4849 809
Size: 507 3533 1802
Fragments arranged by size:
3533 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 2352 3289
Size: 54 1425 50 275 18 291 231 937
Cuts at: 3289 4178 5807 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 937 889 291 275 231 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3289 1537
Size: 275 1477 4090
Fragments arranged by size:
4090 1477 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2190 2835 2982 3772 1353
Size: 837 645 147 790 3423
Fragments arranged by size:
3423 837 790 645 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4429 4803 4949 387
Size: 1064 138 2840 374 146 1280
Fragments arranged by size:
2840 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2019 2680 2019
Size: 661 5181
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2086 2400 2753 3905 4502 5289 5557
Size: 393 454 314 353 1152 597 787 268
Cuts at: 5557 1239
Size: 1524
Fragments arranged by size:
1524 1152 787 597 454 393 353 314
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2667 2667
Size: 5842
EcoO109I rG'GnC_Cy
Cuts at: 2635 2635
Size: 5842
EcoRI G'AATT_C
Cuts at: 3151 3529 3151
Size: 378 5464
EcoRV GAT'ATC
Cuts at: 2233 2233
Size: 5842
FspI TGC'GCA
Cuts at: 4348 4348
Size: 5842
HaeII r_GCGC'y
Cuts at: 1678 4989 1678
Size: 3311 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2705 2737 4483 4515 4557
Size: 32 135 32 1133 32 1746 32 42
Cuts at: 4557 4589 1373
Size: 32 2626
Fragments arranged by size:
2626 1746 1133 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2754 3186 3432 669
Size: 988 281 816 432 246 3079
Fragments arranged by size:
3079 988 816 432 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5842
HpaI GTT'AAC
Cuts at: 3432 3432
Size: 5842
KpnI G_GTAC'C
Cuts at: 3167 3167
Size: 5842
MfeI C'AATT_G
Cuts at: 3441 3441
Size: 5842
MluI A'CGCG_T
Cuts at: 5718 5718
Size: 5842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2886 4956 5140 1305
Size: 1581 2070 184 2007
Fragments arranged by size:
2070 2007 1581 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2123 2354 10
Size: 54 1425 343 291 231 3498
Fragments arranged by size:
3498 1425 343 291 231 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3041 3800 4159 4318 518
Size: 648 1875 759 359 159 2042
Fragments arranged by size:
2042 1875 759 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2014 2164 3008 3258 3935 5083 5328 1606
Size: 408 150 844 250 677 1148 245 2120
Fragments arranged by size:
2120 1148 844 677 408 250 245 150
NcoI C'CATG_G
Cuts at: 513 1065 2004 2124 513
Size: 552 939 120 4231
Fragments arranged by size:
4231 939 552 120
NdeI CA'TA_TG
Cuts at: 387 2338 2620 387
Size: 1951 282 3609
Fragments arranged by size:
3609 1951 282
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5842
NotI GC'GGCC_GC
Cuts at: 3289 3289
Size: 5842
NspI r_CATG'y
Cuts at: 1132 1229 2375 2926 3040 3200 4745 1132
Size: 97 1146 551 114 160 1545 2229
Fragments arranged by size:
2229 1545 1146 551 160 114 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5842
PciI A'CATG_T
Cuts at: 4741 4741
Size: 5842
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5842
PmeI GTTT'AAAC
Cuts at: 3146 3146
Size: 5842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3911 3943 5424 5456 3911
Size: 32 1481 32 4297
Fragments arranged by size:
4297 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2635 2635
Size: 5842
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5842
PsiI TTA'TAA
Cuts at: 3412 3412
Size: 5842
PstI C_TGCA'G
Cuts at: 830 3194 830
Size: 2364 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5842
PvuII CAG'CTG
Cuts at: 2014 2164 3008 2014
Size: 150 844 4848
Fragments arranged by size:
4848 844 150
SacI G_AGCT'C
Cuts at: 2021 2682 2021
Size: 661 5181
SalI G'TCGA_C
Cuts at: 1936 3184 1936
Size: 1248 4594
SbfI CC_TGCA'GG
Cuts at: 3194 3194
Size: 5842
ScaI AGT'ACT
Cuts at: 4090 5802 4090
Size: 1712 4130
SfcI C'TryA_G
Cuts at: 826 1045 3190 4325 5006 5197 826
Size: 219 2145 1135 681 191 1471
Fragments arranged by size:
2145 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5842
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5842
SmaI CCC'GGG
Cuts at: 1872 3169 1872
Size: 1297 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2639 3946 4847 5109 820
Size: 197 926 696 1307 901 262 1553
Fragments arranged by size:
1553 1307 926 901 696 262 197
SnaBI TAC'GTA
Cuts at: 493 3633 493
Size: 3140 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5842
SphI G_CATG'C
Cuts at: 1132 2375 3200 1132
Size: 1243 825 3774
Fragments arranged by size:
3774 1243 825
SspI AAT'ATT
Cuts at: 5 52 3766 5
Size: 47 3714 2081
Fragments arranged by size:
3714 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5842
StyI C'CwwG_G
Cuts at: 513 1065 2004 2040 2124 2668 2875 3281 513
Size: 552 939 36 84 544 207 406 3074
Fragments arranged by size:
3074 939 552 544 406 207 84 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3869 3886 4039 4224 4669 325
Size: 1255 2289 17 153 185 445 1498
Fragments arranged by size:
2289 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4088 5800 96
Size: 274 80 33 51 1443 2111 1712 138
Fragments arranged by size:
2111 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2627 2659 5802 5834 2627
Size: 32 3143 32 2635
Fragments arranged by size:
3143 2635 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5842
XbaI T'CTAG_A
Cuts at: 3178 3178
Size: 5842
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2131 2833 2131
Size: 702 5140
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5842
XmaI C'CCGG_G
Cuts at: 1870 3167 1870
Size: 1297 4545
XmnI GAAnn'nnTTC
Cuts at: 2199 3971 2199
Size: 1772 4070
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3650 276
Size: 53 83 186 936 2116 2468
Fragments arranged by size:
2468 2116 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI