Restriction map for
pFN21AE1851
(Circular) (Six-base) MAPSORT of: pFN21AE1851.seq Check: 7808 from: 1 to: 5815
pFN21AE1851 5815 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3625 278
Size: 53 83 186 936 2089 2468
Fragments arranged by size:
2468 2089 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2874 3158 1937
Size: 937 284 4594
Fragments arranged by size:
4594 937 284
Acc65I G'GTAC_C
Cuts at: 3136 3136
Size: 5815
AclI AA'CG_TT
Cuts at: 1563 3942 4315 1563
Size: 2379 373 3063
Fragments arranged by size:
3063 2379 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3878 5262 5530 1632
Size: 2246 1384 268 1917
Fragments arranged by size:
2246 1917 1384 268
AfeI AGC'GCT
Cuts at: 3035 3035
Size: 5815
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5618
AflIII A'CryG_T
Cuts at: 1203 2459 4714 5691 1203
Size: 1256 2255 977 1327
Fragments arranged by size:
2255 1327 1256 977
AgeI A'CCGG_T
Cuts at: 4646 4646
Size: 5815
AhdI GACnn_n'nnGTC
Cuts at: 4544 4544
Size: 5815
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5815
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5815
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2111 2143 2111
Size: 32 5783
AlwNI CAG_nnn'CTG
Cuts at: 3558 5130 3558
Size: 1572 4243
ApaI G_GGCC'C
Cuts at: 2334 2492 2334
Size: 158 5657
ApaLI G'TGCA_C
Cuts at: 3872 5028 3872
Size: 1156 4659
ApoI r'AATT_y
Cuts at: 1493 3124 3350 3502 5611 1493
Size: 1631 226 152 2109 1697
Fragments arranged by size:
2109 1697 1631 226 152
AscI GG'CGCG_CC
Cuts at: 2941 2941
Size: 5815
AseI AT'TA_AT
Cuts at: 160 1032 4369 160
Size: 872 3337 1606
Fragments arranged by size:
3337 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
AvaI C'yCGr_G
Cuts at: 1870 1943 2174 2483 3140 1870
Size: 73 231 309 657 4545
Fragments arranged by size:
4545 657 309 231 73
BamHI G'GATC_C
Cuts at: 2235 3145 3512 2235
Size: 910 367 4538
Fragments arranged by size:
4538 910 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2759 3136 4591 618
Size: 325 217 627 972 377 1455 1842
Fragments arranged by size:
1842 1455 972 627 377 325 217
BanII G_rGCy'C
Cuts at: 1391 2013 2181 2334 2492 3134 1391
Size: 622 168 153 158 642 4072
Fragments arranged by size:
4072 642 622 168 158 153
BbeI G_GCGC'C
Cuts at: 2763 2763
Size: 5815
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2841 928
Size: 971 942 3902
Fragments arranged by size:
3902 971 942
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4006 4040 1903
Size: 34 2069 34 3678
Fragments arranged by size:
3678 2069 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3707 4917 3707
Size: 1210 4605
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5815
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2487 2544 4426
Size: 107 122 71 631 605 815 57 1882
Cuts at: 4426 136
Size: 1525
Fragments arranged by size:
1882 1525 815 631 605 122 107 71
57
BglII A'GATC_T
Cuts at: 5810 5810
Size: 5815
BlpI GC'TnA_GC
Cuts at: 3232 3232
Size: 5815
Bme1580I G_kGCm'C
Cuts at: 1790 2334 2492 2924 3876 5032 1790
Size: 544 158 432 952 1156 2573
Fragments arranged by size:
2573 1156 952 544 432 158
BmgBI CAC'GTC
Cuts at: 1318 3067 1318
Size: 1749 4066
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2175 2512 3546
Size: 416 53 83 399 24 758 337 1034
Cuts at: 3546 4504 442
Size: 958 1753
Fragments arranged by size:
1753 1034 958 758 416 399 337 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5815
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2310 2846 4475 1239
Size: 1071 536 1629 2579
Fragments arranged by size:
2579 1629 1071 536
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3940 4805 5103 3940
Size: 865 298 4652
Fragments arranged by size:
4652 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4478 882
Size: 617 2979 2219
Fragments arranged by size:
2979 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2460 2697 2904 3606 493
Size: 711 1256 237 207 702 2702
Fragments arranged by size:
2702 1256 711 702 237 207
BsaBI GATnn'nnATC
Cuts at: 5809 5809
Size: 5815
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2212 2760 2914 3622
Size: 53 83 186 936 679 548 154 708
Cuts at: 3622 4004 4639 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 708 679 635 548 382 186
154 83 53
BsaWI w'CCGG_w
Cuts at: 2295 4248 4646 4920 5067 5478 2295
Size: 1953 398 274 147 411 2632
Fragments arranged by size:
2632 1953 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2111 2141 2645 2675 1178
Size: 30 165 30 708 30 504 30 4318
Fragments arranged by size:
4318 708 504 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2119 1186
Size: 933 4882
BseYI C'CCAG_C
Cuts at: 2031 5018 5514 2031
Size: 2987 496 2332
Fragments arranged by size:
2987 2332 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2225 2440 2884 3028 3067 3086 1163
Size: 573 489 215 444 144 39 19 3892
Fragments arranged by size:
3892 573 489 444 215 144 39 19
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2536 2548 2631 2749 3265 4026
Size: 275 186 535 12 83 118 516 761
Cuts at: 4026 5054 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 535 516 275 186 118
83 12
BsiHKAI G_wGCw'C
Cuts at: 3876 3961 5032 3876
Size: 85 1071 4659
Fragments arranged by size:
4659 1071 85
BsiWI C'GTAC_G
Cuts at: 2624 2624
Size: 5815
BsmI GAATG_Cn'
Cuts at: 3326 3419 3326
Size: 93 5722
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2181 2334 2492 2924 3134 3876
Size: 399 223 168 153 158 432 210 742
Cuts at: 3876 3961 5032 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 432 399 223 210 168
158 153 85
BspEI T'CCGG_A
Cuts at: 2295 5478 2295
Size: 3183 2632
BspHI T'CATG_A
Cuts at: 2595 3704 5434 2595
Size: 1109 1730 2976
Fragments arranged by size:
2976 1730 1109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2148 2592 3170 844
Size: 1304 444 578 3489
Fragments arranged by size:
3489 1304 578 444
BsrBI CCG'CTC
Cuts at: 1394 1823 2066 3702 1394
Size: 429 243 1636 3507
Fragments arranged by size:
3507 1636 429 243
BsrDI GCAATG_nn'
Cuts at: 65 1248 4310 4484 65
Size: 1183 3062 174 1396
Fragments arranged by size:
3062 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2469 4459 4646 1700
Size: 110 659 1990 187 2869
Fragments arranged by size:
2869 1990 659 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5815
BssHII G'CGCG_C
Cuts at: 1925 2423 2722 2941 1925
Size: 498 299 219 4799
Fragments arranged by size:
4799 498 299 219
BssSI C'ACGA_G
Cuts at: 3104 3875 4887 3104
Size: 771 1012 4032
Fragments arranged by size:
4032 1012 771
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5815
BstBI TT'CG_AA
Cuts at: 4619 4619
Size: 5815
BstEII G'GTnAC_C
Cuts at: 1184 2407 1184
Size: 1223 4592
BstYI r'GATC_y
Cuts at: 1972 2235 3145 3512 3897 3914 5366 5452 5464
Size: 263 910 367 385 17 1452 86 12
Cuts at: 5464 5810 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 910 385 367 346 263 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2665 2727 2785 513
Size: 552 939 661 62 58 3543
Fragments arranged by size:
3543 939 661 552 62 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2748 487
Size: 683 56 1522 3554
Fragments arranged by size:
3554 1522 683 56
BtsI GCAGTG_nn'
Cuts at: 2101 2319 3331 4125 4145 2101
Size: 218 1012 794 20 3771
Fragments arranged by size:
3771 1012 794 218 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5815
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2077 2112 529
Size: 35 1513 35 4232
Fragments arranged by size:
4232 1513 35 35
DraI TTT'AAA
Cuts at: 3119 3119
Size: 5815
DraIII CAC_nnn'GTG
Cuts at: 2248 2248
Size: 5815
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4822 809
Size: 507 3506 1802
Fragments arranged by size:
3506 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2061 2098 2533
Size: 54 1425 50 275 18 231 37 435
Cuts at: 2533 2545 3262 4151 5780 8
Size: 12 717 889 1629 43
Fragments arranged by size:
1629 1425 889 717 435 275 231 54
50 43 37 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2533 2545 3262 1537
Size: 275 721 12 717 4090
Fragments arranged by size:
4090 721 717 275 12
EarI CTCTTCn'nnn_
Cuts at: 1353 2345 2546 3745 1353
Size: 992 201 1199 3423
Fragments arranged by size:
3423 1199 992 201
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2389 2825 4402 4776 4922 387
Size: 1064 138 800 436 1577 374 146 1280
Fragments arranged by size:
1577 1280 1064 800 436 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2310 2846 3878 4475 5262 5530 1239
Size: 393 678 536 1032 597 787 268 1524
Fragments arranged by size:
1524 1032 787 678 597 536 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2330 2331 2488 2489 2581 2330
Size: 1 157 1 92 5564
Fragments arranged by size:
5564 157 92 1 1
EcoRI G'AATT_C
Cuts at: 3124 3502 3124
Size: 378 5437
FspI TGC'GCA
Cuts at: 2693 4321 2693
Size: 1628 4187
FspAI rTGC'GCAy
Cuts at: 2693 2693
Size: 5815
HaeII r_GCGC'y
Cuts at: 1678 2021 2060 2763 3037 4962 1678
Size: 343 39 703 274 1925 2531
Fragments arranged by size:
2531 1925 703 343 274 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2747 2779 4456 4488 4530
Size: 32 135 32 1175 32 1677 32 42
Cuts at: 4530 4562 1373
Size: 32 2626
Fragments arranged by size:
2626 1677 1175 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2356 3159 3405 669
Size: 988 281 418 803 246 3079
Fragments arranged by size:
3079 988 803 418 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5815
HpaI GTT'AAC
Cuts at: 3405 3405
Size: 5815
KasI G'GCGC_C
Cuts at: 2759 2759
Size: 5815
KpnI G_GTAC'C
Cuts at: 3140 3140
Size: 5815
MfeI C'AATT_G
Cuts at: 3414 3414
Size: 5815
MluI A'CGCG_T
Cuts at: 5691 5691
Size: 5815
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4929 5113 1305
Size: 3624 184 2007
Fragments arranged by size:
3624 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3993
Fragments arranged by size:
3993 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3773 4132 4291 518
Size: 648 2607 359 159 2042
Fragments arranged by size:
2607 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2191 2209 2307 2787 2910 3072 3231 3908
Size: 585 18 98 480 123 162 159 677
Cuts at: 3908 5056 5301 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 585 480 245 162 159
123 98 18
NarI GG'CG_CC
Cuts at: 2760 2760
Size: 5815
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4324
Fragments arranged by size:
4324 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5815
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5815
NotI GC'GGCC_GC
Cuts at: 3262 3262
Size: 5815
NspI r_CATG'y
Cuts at: 1132 1229 3173 4718 1132
Size: 97 1944 1545 2229
Fragments arranged by size:
2229 1944 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5815
PciI A'CATG_T
Cuts at: 4714 4714
Size: 5815
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2419 1108
Size: 1311 4504
PmeI GTTT'AAAC
Cuts at: 3119 3119
Size: 5815
PmlI CAC'GTG
Cuts at: 2460 2697 2460
Size: 237 5578
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2111 2143 3884 3916 5397 5429 2111
Size: 32 1741 32 1481 32 2497
Fragments arranged by size:
2497 1741 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2581 2581
Size: 5815
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5815
PsiI TTA'TAA
Cuts at: 3385 3385
Size: 5815
PspOMI G'GGCC_C
Cuts at: 2330 2488 2330
Size: 158 5657
PspXI vC'TCGA_Gb
Cuts at: 2174 2174
Size: 5815
PstI C_TGCA'G
Cuts at: 830 2190 2265 2516 3167 830
Size: 1360 75 251 651 3478
Fragments arranged by size:
3478 1360 651 251 75
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5815
PvuII CAG'CTG
Cuts at: 2191 3072 2191
Size: 881 4934
SacII CC_GC'GG
Cuts at: 2788 2788
Size: 5815
SalI G'TCGA_C
Cuts at: 1936 3157 1936
Size: 1221 4594
SbfI CC_TGCA'GG
Cuts at: 3167 3167
Size: 5815
ScaI AGT'ACT
Cuts at: 4063 5775 4063
Size: 1712 4103
SfcI C'TryA_G
Cuts at: 826 1045 2186 2261 2512 3163 4298 4979 5170
Size: 219 1141 75 251 651 1135 681 191
Cuts at: 5170 826
Size: 1471
Fragments arranged by size:
1471 1141 1135 681 651 251 219 191
75
SfiI GGCCn_nnn'nGGCC
Cuts at: 2487 2487
Size: 5815
SfoI GGC'GCC
Cuts at: 2761 2761
Size: 5815
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5815
SmaI CCC'GGG
Cuts at: 1872 2485 3142 1872
Size: 613 657 4545
Fragments arranged by size:
4545 657 613
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2174 3919 4820 5082 820
Size: 197 926 231 1745 901 262 1553
Fragments arranged by size:
1745 1553 926 901 262 231 197
SnaBI TAC'GTA
Cuts at: 493 3606 493
Size: 3113 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5815
SphI G_CATG'C
Cuts at: 1132 3173 1132
Size: 2041 3774
SspI AAT'ATT
Cuts at: 5 52 3739 5
Size: 47 3687 2081
Fragments arranged by size:
3687 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5815
StyI C'CwwG_G
Cuts at: 513 1065 2004 3254 513
Size: 552 939 1250 3074
Fragments arranged by size:
3074 1250 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2426 3842 3859 4012 4197 4642 325
Size: 1255 846 1416 17 153 185 445 1498
Fragments arranged by size:
1498 1416 1255 846 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4061 5773 96
Size: 274 80 33 51 1443 2084 1712 138
Fragments arranged by size:
2084 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2645 2657 2677 2689 5775 5807 2645
Size: 12 20 12 3086 32 2653
Fragments arranged by size:
3086 2653 32 20 12 12
Tth111I GACn'n_nGTC
Cuts at: 1316 2870 1316
Size: 1554 4261
XbaI T'CTAG_A
Cuts at: 3151 3151
Size: 5815
XhoI C'TCGA_G
Cuts at: 1943 2174 1943
Size: 231 5584
XmaI C'CCGG_G
Cuts at: 1870 2483 3140 1870
Size: 613 657 4545
Fragments arranged by size:
4545 657 613
XmnI GAAnn'nnTTC
Cuts at: 3944 3944
Size: 5815
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3623 276
Size: 53 83 186 936 2089 2468
Fragments arranged by size:
2468 2089 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacII SalI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsmBI
BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI
NaeI NgoMIV NruI NsiI PacI PfoI PsrI RsrII
SacI SanDI SapI SexAI SrfI SwaI XcmI