Restriction map for
pFN21AE1850
(Circular) (Six-base) MAPSORT of: pFN21AE1850.seq Check: 1066 from: 1 to: 5812
pFN21AE1850 5812 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3622 278
Size: 53 83 186 936 2086 2468
Fragments arranged by size:
2468 2086 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2280 3155 1937
Size: 343 875 4594
Fragments arranged by size:
4594 875 343
Acc65I G'GTAC_C
Cuts at: 2238 3133 2238
Size: 895 4917
AclI AA'CG_TT
Cuts at: 1563 3939 4312 1563
Size: 2376 373 3063
Fragments arranged by size:
3063 2376 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2496 2691 3125 3875 5259 5527 1632
Size: 864 195 434 750 1384 268 1917
Fragments arranged by size:
1917 1384 864 750 434 268 195
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5615
AflIII A'CryG_T
Cuts at: 1203 2683 4711 5688 1203
Size: 1480 2028 977 1327
Fragments arranged by size:
2028 1480 1327 977
AgeI A'CCGG_T
Cuts at: 4643 4643
Size: 5812
AhdI GACnn_n'nnGTC
Cuts at: 4541 4541
Size: 5812
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5812
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5812
AlwNI CAG_nnn'CTG
Cuts at: 3026 3555 5127 3026
Size: 529 1572 3711
Fragments arranged by size:
3711 1572 529
ApaLI G'TGCA_C
Cuts at: 3869 5025 3869
Size: 1156 4656
ApoI r'AATT_y
Cuts at: 1493 3121 3347 3499 5608 1493
Size: 1628 226 152 2109 1697
Fragments arranged by size:
2109 1697 1628 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4366 160
Size: 872 3334 1606
Fragments arranged by size:
3334 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5812
AvaI C'yCGr_G
Cuts at: 1870 1943 3137 1870
Size: 73 1194 4545
Fragments arranged by size:
4545 1194 73
BamHI G'GATC_C
Cuts at: 3142 3509 3142
Size: 367 5445
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2238 2377 3133 4588 618
Size: 325 217 627 451 139 756 1455 1842
Fragments arranged by size:
1842 1455 756 627 451 325 217 139
BanII G_rGCy'C
Cuts at: 1391 2030 3063 3131 1391
Size: 639 1033 68 4072
Fragments arranged by size:
4072 1033 639 68
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4841
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4003 4037 1903
Size: 34 2066 34 3678
Fragments arranged by size:
3678 2066 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3704 4914 3704
Size: 1210 4602
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5812
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4423 136
Size: 107 122 71 631 605 2751 1525
Fragments arranged by size:
2751 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5807 5807
Size: 5812
BlpI GC'TnA_GC
Cuts at: 3229 3229
Size: 5812
Bme1580I G_kGCm'C
Cuts at: 1790 3873 5029 1790
Size: 2083 1156 2573
Fragments arranged by size:
2573 2083 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5812
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3543 4501 442
Size: 416 53 83 399 24 2126 958 1753
Fragments arranged by size:
2126 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5812
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2085 4472 1239
Size: 846 2387 2579
Fragments arranged by size:
2579 2387 846
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3937 4802 5100 3937
Size: 865 298 4649
Fragments arranged by size:
4649 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4475 882
Size: 617 2976 2219
Fragments arranged by size:
2976 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3603 493
Size: 711 2399 2702
Fragments arranged by size:
2702 2399 711
BsaBI GATnn'nnATC
Cuts at: 5806 5806
Size: 5812
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3619 4001 4636 275
Size: 53 83 186 936 2086 382 635 1451
Fragments arranged by size:
2086 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4245 4643 4917 5064 5475 4245
Size: 398 274 147 411 4582
Fragments arranged by size:
4582 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2002 2032 1178
Size: 30 165 30 599 30 4958
Fragments arranged by size:
4958 599 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2020 1186
Size: 834 4978
BseYI C'CCAG_C
Cuts at: 2023 2056 3019 5015 5511 2023
Size: 33 963 1996 496 2324
Fragments arranged by size:
2324 1996 963 496 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3061 1163
Size: 573 1325 3914
Fragments arranged by size:
3914 1325 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3262 4023 5051 1540
Size: 275 186 1261 761 1028 2301
Fragments arranged by size:
2301 1261 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3063 3873 3958 5029 3063
Size: 810 85 1071 3846
Fragments arranged by size:
3846 1071 810 85
BsmI GAATG_Cn'
Cuts at: 2880 2928 3323 3416 2880
Size: 48 395 93 5276
Fragments arranged by size:
5276 395 93 48
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 3063 3131 3873 3958 5029 1391
Size: 399 240 1033 68 742 85 1071 2174
Fragments arranged by size:
2174 1071 1033 742 399 240 85 68
BspEI T'CCGG_A
Cuts at: 5475 5475
Size: 5812
BspHI T'CATG_A
Cuts at: 3701 5431 3701
Size: 1730 4082
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2807 3167 844
Size: 1963 360 3489
Fragments arranged by size:
3489 1963 360
BsrBI CCG'CTC
Cuts at: 1394 1823 3699 1394
Size: 429 1876 3507
Fragments arranged by size:
3507 1876 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4307 4481 65
Size: 1183 3059 174 1396
Fragments arranged by size:
3059 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4456 4643 1700
Size: 110 2646 187 2869
Fragments arranged by size:
2869 2646 187 110
BsrGI T'GTAC_A
Cuts at: 96 2256 96
Size: 2160 3652
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5812
BssSI C'ACGA_G
Cuts at: 3872 4884 3872
Size: 1012 4800
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2990 794
Size: 2196 3616
BstBI TT'CG_AA
Cuts at: 4616 4616
Size: 5812
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5812
BstXI CCAn_nnnn'nTGG
Cuts at: 2132 2132
Size: 5812
BstYI r'GATC_y
Cuts at: 1972 3142 3509 3894 3911 5363 5449 5461 5807
Size: 1170 367 385 17 1452 86 12 346
Cuts at: 5807 1972
Size: 1977
Fragments arranged by size:
1977 1452 1170 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2372 2372
Size: 5812
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5260
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5073
Fragments arranged by size:
5073 683 56
BtsI GCAGTG_nn'
Cuts at: 3328 4122 4142 3328
Size: 794 20 4998
Fragments arranged by size:
4998 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5812
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5777
DraI TTT'AAA
Cuts at: 3042 3116 3042
Size: 74 5738
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4819 809
Size: 507 3503 1802
Fragments arranged by size:
3503 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2014 3259 4148
Size: 54 1425 50 275 18 184 1245 889
Cuts at: 4148 5777 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1245 889 275 184 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3259 1537
Size: 275 1447 4090
Fragments arranged by size:
4090 1447 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2358 2616 2628 3051 3742 1353
Size: 1005 258 12 423 691 3423
Fragments arranged by size:
3423 1005 691 423 258 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4399 4773 4919 387
Size: 1064 138 2810 374 146 1280
Fragments arranged by size:
2810 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3061 3061
Size: 5812
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2085 2496 2691 3125 3875 4472 5259
Size: 393 453 411 195 434 750 597 787
Cuts at: 5259 5527 1239
Size: 268 1524
Fragments arranged by size:
1524 787 750 597 453 434 411 393
268 195
EcoO109I rG'GnC_Cy
Cuts at: 2060 2060
Size: 5812
EcoRI G'AATT_C
Cuts at: 3121 3499 3121
Size: 378 5434
FspI TGC'GCA
Cuts at: 4318 4318
Size: 5812
HaeII r_GCGC'y
Cuts at: 1678 4959 1678
Size: 3281 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4453 4485 4527 4559 1373
Size: 32 135 32 2881 32 42 32 2626
Fragments arranged by size:
2881 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2281 2484 3156 3402 669
Size: 988 281 343 203 672 246 3079
Fragments arranged by size:
3079 988 672 343 281 246 203
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5812
HpaI GTT'AAC
Cuts at: 2484 3402 2484
Size: 918 4894
KpnI G_GTAC'C
Cuts at: 2242 3137 2242
Size: 895 4917
MfeI C'AATT_G
Cuts at: 3411 3411
Size: 5812
MluI A'CGCG_T
Cuts at: 5688 5688
Size: 5812
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4926 5110 1305
Size: 3621 184 2007
Fragments arranged by size:
3621 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3990
Fragments arranged by size:
3990 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2958 3770 4129 4288 518
Size: 648 1792 812 359 159 2042
Fragments arranged by size:
2042 1792 812 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2286 3089 3228 3905 5053 5298 1606
Size: 680 803 139 677 1148 245 2120
Fragments arranged by size:
2120 1148 803 680 677 245 139
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5260
NdeI CA'TA_TG
Cuts at: 387 2180 387
Size: 1793 4019
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5812
NotI GC'GGCC_GC
Cuts at: 3259 3259
Size: 5812
NspI r_CATG'y
Cuts at: 1132 1229 2687 3170 4715 1132
Size: 97 1458 483 1545 2229
Fragments arranged by size:
2229 1545 1458 483 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5812
PciI A'CATG_T
Cuts at: 2683 4711 2683
Size: 2028 3784
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5812
PfoI T'CCnGG_A
Cuts at: 2583 3009 2583
Size: 426 5386
PmeI GTTT'AAAC
Cuts at: 3116 3116
Size: 5812
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3881 3913 5394 5426 3881
Size: 32 1481 32 4267
Fragments arranged by size:
4267 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5812
PsiI TTA'TAA
Cuts at: 3382 3382
Size: 5812
PstI C_TGCA'G
Cuts at: 830 2644 3164 830
Size: 1814 520 3478
Fragments arranged by size:
3478 1814 520
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5812
PvuII CAG'CTG
Cuts at: 3089 3089
Size: 5812
SacI G_AGCT'C
Cuts at: 3063 3063
Size: 5812
SalI G'TCGA_C
Cuts at: 1936 2279 3154 1936
Size: 343 875 4594
Fragments arranged by size:
4594 875 343
SapI GCTCTTCn'nnn_
Cuts at: 3051 3051
Size: 5812
SbfI CC_TGCA'GG
Cuts at: 3164 3164
Size: 5812
ScaI AGT'ACT
Cuts at: 2263 4060 5772 2263
Size: 1797 1712 2303
Fragments arranged by size:
2303 1797 1712
SfcI C'TryA_G
Cuts at: 826 1045 2141 2194 2640 3160 4295 4976 5167
Size: 219 1096 53 446 520 1135 681 191
Cuts at: 5167 826
Size: 1471
Fragments arranged by size:
1471 1135 1096 681 520 446 219 191
53
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5812
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5812
SmaI CCC'GGG
Cuts at: 1872 3139 1872
Size: 1267 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3916 4817 5079 820
Size: 197 926 1973 901 262 1553
Fragments arranged by size:
1973 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3603 493
Size: 3110 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5812
SphI G_CATG'C
Cuts at: 1132 3170 1132
Size: 2038 3774
SspI AAT'ATT
Cuts at: 5 52 3736 5
Size: 47 3684 2081
Fragments arranged by size:
3684 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5812
StyI C'CwwG_G
Cuts at: 513 1065 2243 2787 3251 513
Size: 552 1178 544 464 3074
Fragments arranged by size:
3074 1178 552 544 464
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3033 3839 3856 4009 4194 4639 325
Size: 1255 1453 806 17 153 185 445 1498
Fragments arranged by size:
1498 1453 1255 806 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2256 2261 2601
Size: 274 80 33 51 1443 279 5 340
Cuts at: 2601 4058 5770 96
Size: 1457 1712 138
Fragments arranged by size:
1712 1457 1443 340 279 274 138 80
51 33 5
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5772 5804 5772
Size: 32 5780
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5812
XbaI T'CTAG_A
Cuts at: 2695 3148 2695
Size: 453 5359
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5812
XmaI C'CCGG_G
Cuts at: 1870 3137 1870
Size: 1267 4545
XmnI GAAnn'nnTTC
Cuts at: 3941 3941
Size: 5812
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3620 276
Size: 53 83 186 936 2086 2468
Fragments arranged by size:
2468 2086 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI