Restriction map for
pFN21AE1847
(Circular) (Six-base) MAPSORT of: pFN21AE1847.seq Check: 3194 from: 1 to: 5800
pFN21AE1847 5800 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3610 278
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2661 3143 1937
Size: 724 482 4594
Fragments arranged by size:
4594 724 482
Acc65I G'GTAC_C
Cuts at: 3121 3121
Size: 5800
AclI AA'CG_TT
Cuts at: 1563 3927 4300 1563
Size: 2364 373 3063
Fragments arranged by size:
3063 2364 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3863 5247 5515 1632
Size: 2231 1384 268 1917
Fragments arranged by size:
2231 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5603
AflIII A'CryG_T
Cuts at: 1203 4699 5676 1203
Size: 3496 977 1327
Fragments arranged by size:
3496 1327 977
AgeI A'CCGG_T
Cuts at: 4631 4631
Size: 5800
AhdI GACnn_n'nnGTC
Cuts at: 4529 4529
Size: 5800
AleI CACnn'nnGTG
Cuts at: 1166 2999 1166
Size: 1833 3967
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2977 1184
Size: 1793 4007
AlwNI CAG_nnn'CTG
Cuts at: 3543 5115 3543
Size: 1572 4228
ApaLI G'TGCA_C
Cuts at: 3857 5013 3857
Size: 1156 4644
ApoI r'AATT_y
Cuts at: 1493 3109 3335 3487 5596 1493
Size: 1616 226 152 2109 1697
Fragments arranged by size:
2109 1697 1616 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4354 160
Size: 872 3322 1606
Fragments arranged by size:
3322 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
AvaI C'yCGr_G
Cuts at: 1870 1943 3125 1870
Size: 73 1182 4545
Fragments arranged by size:
4545 1182 73
BamHI G'GATC_C
Cuts at: 3130 3497 3130
Size: 367 5433
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2222 2315 2351 3121 4576
Size: 325 217 627 435 93 36 770 1455
Cuts at: 4576 618
Size: 1842
Fragments arranged by size:
1842 1455 770 627 435 325 217 93
36
BanII G_rGCy'C
Cuts at: 1391 2086 3119 1391
Size: 695 1033 4072
Fragments arranged by size:
4072 1033 695
BbeI G_GCGC'C
Cuts at: 2226 2319 2226
Size: 93 5707
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2715 928
Size: 971 816 4013
Fragments arranged by size:
4013 971 816
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3991 4025 1903
Size: 34 2054 34 3678
Fragments arranged by size:
3678 2054 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2783 3692 4902 2783
Size: 909 1210 3681
Fragments arranged by size:
3681 1210 909
BclI T'GATC_A
Cuts at: 1548 2283 1548
Size: 735 5065
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4411 136
Size: 107 122 71 631 605 2739 1525
Fragments arranged by size:
2739 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5795 5795
Size: 5800
BlpI GC'TnA_GC
Cuts at: 2342 2476 3217 2342
Size: 134 741 4925
Fragments arranged by size:
4925 741 134
Bme1580I G_kGCm'C
Cuts at: 1790 3861 5017 1790
Size: 2071 1156 2573
Fragments arranged by size:
2573 2071 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5800
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2506 2912 3531
Size: 416 53 83 399 24 1089 406 619
Cuts at: 3531 4489 442
Size: 958 1753
Fragments arranged by size:
1753 1089 958 619 416 406 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4460 1239
Size: 3221 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3925 4790 5088 3925
Size: 865 298 4637
Fragments arranged by size:
4637 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4463 882
Size: 617 2964 2219
Fragments arranged by size:
2964 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3013 3591 493
Size: 711 1809 578 2702
Fragments arranged by size:
2702 1809 711 578
BsaBI GATnn'nnATC
Cuts at: 5794 5794
Size: 5800
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2223 2316 3607 3989
Size: 53 83 186 936 690 93 1291 382
Cuts at: 3989 4624 275
Size: 635 1451
Fragments arranged by size:
1451 1291 936 690 635 382 186 93
83 53
BsaWI w'CCGG_w
Cuts at: 4233 4631 4905 5052 5463 4233
Size: 398 274 147 411 4570
Fragments arranged by size:
4570 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5575
Fragments arranged by size:
5575 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2960 1186
Size: 1774 4026
BseYI C'CCAG_C
Cuts at: 2137 2230 5003 5499 2137
Size: 93 2773 496 2438
Fragments arranged by size:
2773 2438 496 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2445 2973 1163
Size: 573 709 528 3990
Fragments arranged by size:
3990 709 573 528
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3250 4011 5039 1540
Size: 275 186 1249 761 1028 2301
Fragments arranged by size:
2301 1249 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3861 3946 5017 3861
Size: 85 1071 4644
Fragments arranged by size:
4644 1071 85
BsmI GAATG_Cn'
Cuts at: 2235 2401 2936 3311 3404 2235
Size: 166 535 375 93 4631
Fragments arranged by size:
4631 535 375 166 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2086 3119 3861 3946 5017 1391
Size: 399 296 1033 742 85 1071 2174
Fragments arranged by size:
2174 1071 1033 742 399 296 85
BspEI T'CCGG_A
Cuts at: 5463 5463
Size: 5800
BspHI T'CATG_A
Cuts at: 3689 5419 3689
Size: 1730 4070
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2208 3155 844
Size: 1364 947 3489
Fragments arranged by size:
3489 1364 947
BsrBI CCG'CTC
Cuts at: 1394 1823 3687 1394
Size: 429 1864 3507
Fragments arranged by size:
3507 1864 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2109 2610 4295 4469 65
Size: 1183 861 501 1685 174 1396
Fragments arranged by size:
1685 1396 1183 861 501 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4444 4631 1700
Size: 110 2634 187 2869
Fragments arranged by size:
2869 2634 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5800
BssHII G'CGCG_C
Cuts at: 1925 2043 1925
Size: 118 5682
BssSI C'ACGA_G
Cuts at: 2063 2264 3860 4872 2063
Size: 201 1596 1012 2991
Fragments arranged by size:
2991 1596 1012 201
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2931 794
Size: 2137 3663
BstBI TT'CG_AA
Cuts at: 4604 4604
Size: 5800
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5800
BstYI r'GATC_y
Cuts at: 1972 3130 3497 3882 3899 5351 5437 5449 5795
Size: 1158 367 385 17 1452 86 12 346
Cuts at: 5795 1972
Size: 1977
Fragments arranged by size:
1977 1452 1158 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2574 2674 513
Size: 552 939 570 100 3639
Fragments arranged by size:
3639 939 570 552 100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5061
Fragments arranged by size:
5061 683 56
BtsI GCAGTG_nn'
Cuts at: 2428 3316 4110 4130 2428
Size: 888 794 20 4098
Fragments arranged by size:
4098 888 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5800
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5765
DraI TTT'AAA
Cuts at: 3104 3104
Size: 5800
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4807 809
Size: 507 3491 1802
Fragments arranged by size:
3491 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2740 2980 3247
Size: 54 1425 50 275 18 910 240 267
Cuts at: 3247 4136 5765 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 910 889 275 267 240 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3247 1537
Size: 275 1435 4090
Fragments arranged by size:
4090 1435 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3730 1353
Size: 2377 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4387 4761 4907 387
Size: 1064 138 2798 374 146 1280
Fragments arranged by size:
2798 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3863 4460 5247 5515 1239
Size: 393 2231 597 787 268 1524
Fragments arranged by size:
2231 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3109 3487 3109
Size: 378 5422
EcoRV GAT'ATC
Cuts at: 2781 2781
Size: 5800
FspI TGC'GCA
Cuts at: 2807 4306 2807
Size: 1499 4301
HaeII r_GCGC'y
Cuts at: 1678 2021 2226 2319 4947 1678
Size: 343 205 93 2628 2531
Fragments arranged by size:
2628 2531 343 205 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4441 4473 4515 4547 1373
Size: 32 135 32 2869 32 42 32 2626
Fragments arranged by size:
2869 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3144 3390 669
Size: 988 281 1206 246 3079
Fragments arranged by size:
3079 1206 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5800
HpaI GTT'AAC
Cuts at: 3390 3390
Size: 5800
KasI G'GCGC_C
Cuts at: 2222 2315 2222
Size: 93 5707
KpnI G_GTAC'C
Cuts at: 3125 3125
Size: 5800
MfeI C'AATT_G
Cuts at: 3399 3399
Size: 5800
MluI A'CGCG_T
Cuts at: 5676 5676
Size: 5800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4914 5098 1305
Size: 3609 184 2007
Fragments arranged by size:
3609 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2742 10
Size: 54 1425 343 910 3068
Fragments arranged by size:
3068 1425 910 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2619 2847 2999 3758 4117 4276 518
Size: 648 1453 228 152 759 359 159 2042
Fragments arranged by size:
2042 1453 759 648 359 228 159 152
MspA1I CmG'CkG
Cuts at: 1606 2158 2349 2670 3216 3893 5041 5286 1606
Size: 552 191 321 546 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 552 546 321 245 191
NarI GG'CG_CC
Cuts at: 2223 2316 2223
Size: 93 5707
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4309
Fragments arranged by size:
4309 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5800
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5800
NotI GC'GGCC_GC
Cuts at: 3247 3247
Size: 5800
NspI r_CATG'y
Cuts at: 1132 1229 2533 2709 3158 4703 1132
Size: 97 1304 176 449 1545 2229
Fragments arranged by size:
2229 1545 1304 449 176 97
PasI CC'CwG_GG
Cuts at: 1348 2912 1348
Size: 1564 4236
PciI A'CATG_T
Cuts at: 4699 4699
Size: 5800
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5800
PmeI GTTT'AAAC
Cuts at: 3104 3104
Size: 5800
PmlI CAC'GTG
Cuts at: 3013 3013
Size: 5800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2459 2491 3017 3049 3869 3901 5382 5414 2459
Size: 32 526 32 820 32 1481 32 2845
Fragments arranged by size:
2845 1481 820 526 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3083 1613
Size: 1470 4330
PsiI TTA'TAA
Cuts at: 3370 3370
Size: 5800
PstI C_TGCA'G
Cuts at: 830 2392 3152 830
Size: 1562 760 3478
Fragments arranged by size:
3478 1562 760
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5800
SalI G'TCGA_C
Cuts at: 1936 3142 1936
Size: 1206 4594
SbfI CC_TGCA'GG
Cuts at: 3152 3152
Size: 5800
ScaI AGT'ACT
Cuts at: 2595 4048 5760 2595
Size: 1453 1712 2635
Fragments arranged by size:
2635 1712 1453
SexAI A'CCwGG_T
Cuts at: 2411 2411
Size: 5800
SfcI C'TryA_G
Cuts at: 826 1045 2388 3148 4283 4964 5155 826
Size: 219 1343 760 1135 681 191 1471
Fragments arranged by size:
1471 1343 1135 760 681 219 191
SfoI GGC'GCC
Cuts at: 2224 2317 2224
Size: 93 5707
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5800
SmaI CCC'GGG
Cuts at: 1872 3127 1872
Size: 1255 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3904 4805 5067 820
Size: 197 926 1961 901 262 1553
Fragments arranged by size:
1961 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3591 493
Size: 3098 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5800
SphI G_CATG'C
Cuts at: 1132 3158 1132
Size: 2026 3774
SspI AAT'ATT
Cuts at: 5 52 3724 5
Size: 47 3672 2081
Fragments arranged by size:
3672 2081 47
StuI AGG'CCT
Cuts at: 1517 2394 1517
Size: 877 4923
StyI C'CwwG_G
Cuts at: 513 1065 2004 2086 3239 513
Size: 552 939 82 1153 3074
Fragments arranged by size:
3074 1153 939 552 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3011 3827 3844 3997 4182 4627 325
Size: 1255 1431 816 17 153 185 445 1498
Fragments arranged by size:
1498 1431 1255 816 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2593 4046 5758
Size: 274 80 33 51 1443 616 1453 1712
Cuts at: 5758 96
Size: 138
Fragments arranged by size:
1712 1453 1443 616 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2666 2698 5760 5792 2666
Size: 32 3062 32 2674
Fragments arranged by size:
3062 2674 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2413 1316
Size: 1097 4703
XbaI T'CTAG_A
Cuts at: 3136 3136
Size: 5800
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5800
XmaI C'CCGG_G
Cuts at: 1870 3125 1870
Size: 1255 4545
XmnI GAAnn'nnTTC
Cuts at: 3929 3929
Size: 5800
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3608 276
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SfiI
SrfI SwaI XcmI