Restriction map for
pFN21AE1844
(Circular) (Six-base) MAPSORT of: pFN21AE1844.seq Check: 6674 from: 1 to: 5788
pFN21AE1844 5788 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3598 278
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3131 1937
Size: 1194 4594
Acc65I G'GTAC_C
Cuts at: 2398 3109 2398
Size: 711 5077
AclI AA'CG_TT
Cuts at: 1563 3915 4288 1563
Size: 2352 373 3063
Fragments arranged by size:
3063 2352 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2285 2348 2708 3851 5235 5503 1632
Size: 653 63 360 1143 1384 268 1917
Fragments arranged by size:
1917 1384 1143 653 360 268 63
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5591
AflIII A'CryG_T
Cuts at: 1203 3085 4687 5664 1203
Size: 1882 1602 977 1327
Fragments arranged by size:
1882 1602 1327 977
AgeI A'CCGG_T
Cuts at: 4619 4619
Size: 5788
AhdI GACnn_n'nnGTC
Cuts at: 2391 4517 2391
Size: 2126 3662
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5788
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5788
AlwNI CAG_nnn'CTG
Cuts at: 2145 2629 3531 5103 2145
Size: 484 902 1572 2830
Fragments arranged by size:
2830 1572 902 484
ApaI G_GGCC'C
Cuts at: 2880 2880
Size: 5788
ApaLI G'TGCA_C
Cuts at: 3845 5001 3845
Size: 1156 4632
ApoI r'AATT_y
Cuts at: 1493 2345 3097 3323 3475 5584 1493
Size: 852 752 226 152 2109 1697
Fragments arranged by size:
2109 1697 852 752 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4342 160
Size: 872 3310 1606
Fragments arranged by size:
3310 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
AvaI C'yCGr_G
Cuts at: 1870 1943 2253 2952 3113 1870
Size: 73 310 699 161 4545
Fragments arranged by size:
4545 699 310 161 73
BamHI G'GATC_C
Cuts at: 2031 3118 3485 2031
Size: 1087 367 4334
Fragments arranged by size:
4334 1087 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2398 2422 3109 4564 618
Size: 325 217 627 611 24 687 1455 1842
Fragments arranged by size:
1842 1455 687 627 611 325 217 24
BanII G_rGCy'C
Cuts at: 1391 2880 3107 1391
Size: 1489 227 4072
Fragments arranged by size:
4072 1489 227
BbeI G_GCGC'C
Cuts at: 2426 2426
Size: 5788
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2472 928
Size: 971 573 4244
Fragments arranged by size:
4244 971 573
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2600 2634 3979 4013 1903
Size: 34 663 34 1345 34 3678
Fragments arranged by size:
3678 1345 663 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2019 3680 4890 2019
Size: 1661 1210 2917
Fragments arranged by size:
2917 1661 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5788
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4399 136
Size: 107 122 71 631 605 2727 1525
Fragments arranged by size:
2727 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5783 5783
Size: 5788
BlpI GC'TnA_GC
Cuts at: 2670 3205 2670
Size: 535 5253
Bme1580I G_kGCm'C
Cuts at: 1790 2880 3849 5005 1790
Size: 1090 969 1156 2573
Fragments arranged by size:
2573 1156 1090 969
BmgBI CAC'GTC
Cuts at: 1318 2156 2317 1318
Size: 838 161 4789
Fragments arranged by size:
4789 838 161
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2399 2874 3519
Size: 416 53 83 399 24 982 475 645
Cuts at: 3519 4477 442
Size: 958 1753
Fragments arranged by size:
1753 982 958 645 475 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4448 1239
Size: 3209 2579
Bpu10I CC'TnA_GC
Cuts at: 2036 2036
Size: 5788
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2828 2996 3913 4778 5076 2828
Size: 168 917 865 298 3540
Fragments arranged by size:
3540 917 865 298 168
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3037 4451 882
Size: 617 1538 1414 2219
Fragments arranged by size:
2219 1538 1414 617
BsaAI yAC'GTr
Cuts at: 493 1204 3579 493
Size: 711 2375 2702
Fragments arranged by size:
2702 2375 711
BsaBI GATnn'nnATC
Cuts at: 5782 5782
Size: 5788
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2423 3595 3977 4612
Size: 53 83 186 936 890 1172 382 635
Cuts at: 4612 275
Size: 1451
Fragments arranged by size:
1451 1172 936 890 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2089 2395 2401 4221 4619 4893 5040 5451 2089
Size: 306 6 1820 398 274 147 411 2426
Fragments arranged by size:
2426 1820 411 398 306 274 147 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2520 2550 1178
Size: 30 165 30 1117 30 4416
Fragments arranged by size:
4416 1117 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5788
BseYI C'CCAG_C
Cuts at: 4991 5487 4991
Size: 496 5292
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5215
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3238 3999 5027 1540
Size: 275 186 1237 761 1028 2301
Fragments arranged by size:
2301 1237 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2686 3849 3934 5005 2686
Size: 1163 85 1071 3469
Fragments arranged by size:
3469 1163 1071 85
BsmI GAATG_Cn'
Cuts at: 2764 3299 3392 2764
Size: 535 93 5160
Fragments arranged by size:
5160 535 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2686 2880 3107 3849 3934 5005 1391
Size: 399 896 194 227 742 85 1071 2174
Fragments arranged by size:
2174 1071 896 742 399 227 194 85
BspEI T'CCGG_A
Cuts at: 5451 5451
Size: 5788
BspHI T'CATG_A
Cuts at: 3677 5407 3677
Size: 1730 4058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3143 844
Size: 2299 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2185 3675 1394
Size: 429 362 1490 3507
Fragments arranged by size:
3507 1490 429 362
BsrDI GCAATG_nn'
Cuts at: 65 1248 2339 4283 4457 65
Size: 1183 1091 1944 174 1396
Fragments arranged by size:
1944 1396 1183 1091 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4432 4619 1700
Size: 110 2622 187 2869
Fragments arranged by size:
2869 2622 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5788
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5788
BssSI C'ACGA_G
Cuts at: 3848 4860 3848
Size: 1012 4776
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2519 794
Size: 1725 4063
BstBI TT'CG_AA
Cuts at: 4592 4592
Size: 5788
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5788
BstXI CCAn_nnnn'nTGG
Cuts at: 2912 2912
Size: 5788
BstYI r'GATC_y
Cuts at: 1972 2031 2356 2414 3118 3485 3870 3887 5339
Size: 59 325 58 704 367 385 17 1452
Cuts at: 5339 5425 5437 5783 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 704 385 367 346 325 86
59 58 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2649 513
Size: 552 1584 3652
Fragments arranged by size:
3652 1584 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5049
Fragments arranged by size:
5049 683 56
BtsI GCAGTG_nn'
Cuts at: 2520 3304 4098 4118 2520
Size: 784 794 20 4190
Fragments arranged by size:
4190 794 784 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5788
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5753
DraI TTT'AAA
Cuts at: 3092 3092
Size: 5788
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4795 809
Size: 507 3479 1802
Fragments arranged by size:
3479 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2589 3235 4124
Size: 54 1425 50 275 18 759 646 889
Cuts at: 4124 5753 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 759 646 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3235 1537
Size: 275 1423 4090
Fragments arranged by size:
4090 1423 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2723 3718 1353
Size: 1370 995 3423
Fragments arranged by size:
3423 1370 995
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4375 4749 4895 387
Size: 1064 138 2786 374 146 1280
Fragments arranged by size:
2786 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2285 2348 2708 3851 4448 5235 5503
Size: 393 653 63 360 1143 597 787 268
Cuts at: 5503 1239
Size: 1524
Fragments arranged by size:
1524 1143 787 653 597 393 360 268
63
EcoO109I rG'GnC_Cy
Cuts at: 2876 2876
Size: 5788
EcoRI G'AATT_C
Cuts at: 2345 3097 3475 2345
Size: 752 378 4658
Fragments arranged by size:
4658 752 378
FspI TGC'GCA
Cuts at: 4294 4294
Size: 5788
HaeII r_GCGC'y
Cuts at: 1678 2021 2426 4935 1678
Size: 343 405 2509 2531
Fragments arranged by size:
2531 2509 405 343
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2161 2183 2189 2193 2215
Size: 32 135 32 589 22 6 4 22
Cuts at: 2215 2221 2377 2409 2479 2511 2957 2989 4429
Size: 6 156 32 70 32 446 32 1440
Cuts at: 4429 4461 4503 4535 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1440 589 446 156 135 70 42
32 32 32 32 32 32 32 22
22 6 6 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3132 3378 669
Size: 988 281 1194 246 3079
Fragments arranged by size:
3079 1194 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5788
HpaI GTT'AAC
Cuts at: 3378 3378
Size: 5788
KasI G'GCGC_C
Cuts at: 2422 2422
Size: 5788
KpnI G_GTAC'C
Cuts at: 2402 3113 2402
Size: 711 5077
MfeI C'AATT_G
Cuts at: 3387 3387
Size: 5788
MluI A'CGCG_T
Cuts at: 5664 5664
Size: 5788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2181 4902 5086 1305
Size: 876 2721 184 2007
Fragments arranged by size:
2721 2007 876 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2591 10
Size: 54 1425 343 759 3207
Fragments arranged by size:
3207 1425 759 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3746 4105 4264 518
Size: 648 2580 359 159 2042
Fragments arranged by size:
2580 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3204 3881 5029 5274 1606
Size: 1598 677 1148 245 2120
Fragments arranged by size:
2120 1598 1148 677 245
NarI GG'CG_CC
Cuts at: 2423 2423
Size: 5788
NcoI C'CATG_G
Cuts at: 513 1065 2649 513
Size: 552 1584 3652
Fragments arranged by size:
3652 1584 552
NdeI CA'TA_TG
Cuts at: 387 2751 387
Size: 2364 3424
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5788
NotI GC'GGCC_GC
Cuts at: 3235 3235
Size: 5788
NspI r_CATG'y
Cuts at: 1132 1229 3146 4691 1132
Size: 97 1917 1545 2229
Fragments arranged by size:
2229 1917 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5788
PciI A'CATG_T
Cuts at: 4687 4687
Size: 5788
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5788
PmeI GTTT'AAAC
Cuts at: 3092 3092
Size: 5788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3857 3889 5370 5402 3857
Size: 32 1481 32 4243
Fragments arranged by size:
4243 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5788
PsiI TTA'TAA
Cuts at: 3358 3358
Size: 5788
PspOMI G'GGCC_C
Cuts at: 2876 2876
Size: 5788
PstI C_TGCA'G
Cuts at: 830 2607 2634 3140 830
Size: 1777 27 506 3478
Fragments arranged by size:
3478 1777 506 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5788
SalI G'TCGA_C
Cuts at: 1936 3130 1936
Size: 1194 4594
SbfI CC_TGCA'GG
Cuts at: 3140 3140
Size: 5788
ScaI AGT'ACT
Cuts at: 4036 5748 4036
Size: 1712 4076
SfcI C'TryA_G
Cuts at: 826 1045 2025 2074 2603 2630 3136 4271 4952
Size: 219 980 49 529 27 506 1135 681
Cuts at: 4952 5143 826
Size: 191 1471
Fragments arranged by size:
1471 1135 980 681 529 506 219 191
49 27
SfoI GGC'GCC
Cuts at: 2424 2424
Size: 5788
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5788
SmaI CCC'GGG
Cuts at: 1872 3115 1872
Size: 1243 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2807 3011 3892 4793 5055 820
Size: 197 926 864 204 881 901 262 1553
Fragments arranged by size:
1553 926 901 881 864 262 204 197
SnaBI TAC'GTA
Cuts at: 493 3579 493
Size: 3086 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5788
SphI G_CATG'C
Cuts at: 1132 3146 1132
Size: 2014 3774
SspI AAT'ATT
Cuts at: 5 52 3712 5
Size: 47 3660 2081
Fragments arranged by size:
3660 2081 47
StuI AGG'CCT
Cuts at: 1517 2274 3009 3019 1517
Size: 757 735 10 4286
Fragments arranged by size:
4286 757 735 10
StyI C'CwwG_G
Cuts at: 513 1065 2649 3227 513
Size: 552 1584 578 3074
Fragments arranged by size:
3074 1584 578 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3815 3832 3985 4170 4615 325
Size: 1255 2235 17 153 185 445 1498
Fragments arranged by size:
2235 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4034 5746 96
Size: 274 80 33 51 1443 2057 1712 138
Fragments arranged by size:
2057 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5748 5780 5748
Size: 32 5756
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5788
XbaI T'CTAG_A
Cuts at: 3124 3124
Size: 5788
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5788
XmaI C'CCGG_G
Cuts at: 1870 3113 1870
Size: 1243 4545
XmnI GAAnn'nnTTC
Cuts at: 2783 3917 2783
Size: 1134 4654
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3596 276
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI
XcmI