Restriction map for
pFN21AE1841
(Circular) (Six-base) MAPSORT of: pFN21AE1841.seq Check: 7000 from: 1 to: 5776
pFN21AE1841 5776 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3586 278
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2186 3030 3119 1937
Size: 249 844 89 4594
Fragments arranged by size:
4594 844 249 89
Acc65I G'GTAC_C
Cuts at: 3097 3097
Size: 5776
AclI AA'CG_TT
Cuts at: 1563 2782 3903 4276 1563
Size: 1219 1121 373 3063
Fragments arranged by size:
3063 1219 1121 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2898 3839 5223 5491 1632
Size: 1266 941 1384 268 1917
Fragments arranged by size:
1917 1384 1266 941 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5579
AflIII A'CryG_T
Cuts at: 1203 2591 4675 5652 1203
Size: 1388 2084 977 1327
Fragments arranged by size:
2084 1388 1327 977
AgeI A'CCGG_T
Cuts at: 4607 4607
Size: 5776
AhdI GACnn_n'nnGTC
Cuts at: 4505 4505
Size: 5776
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5776
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5776
AlwNI CAG_nnn'CTG
Cuts at: 3519 5091 3519
Size: 1572 4204
ApaI G_GGCC'C
Cuts at: 2200 2200
Size: 5776
ApaLI G'TGCA_C
Cuts at: 3833 4989 3833
Size: 1156 4620
ApoI r'AATT_y
Cuts at: 1493 2621 2998 3085 3311 3463 5572 1493
Size: 1128 377 87 226 152 2109 1697
Fragments arranged by size:
2109 1697 1128 377 226 152 87
AseI AT'TA_AT
Cuts at: 160 1032 2628 4330 160
Size: 872 1596 1702 1606
Fragments arranged by size:
1702 1606 1596 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
AvaI C'yCGr_G
Cuts at: 1870 1943 3101 1870
Size: 73 1158 4545
Fragments arranged by size:
4545 1158 73
BamHI G'GATC_C
Cuts at: 3106 3473 3106
Size: 367 5409
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3097 4552 618
Size: 325 217 627 1310 1455 1842
Fragments arranged by size:
1842 1455 1310 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2142 2200 3095 1391
Size: 751 58 895 4072
Fragments arranged by size:
4072 895 751 58
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4805
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3967 4001 1903
Size: 34 2030 34 3678
Fragments arranged by size:
3678 2030 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3668 4878 3668
Size: 1210 4566
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5776
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4387 136
Size: 107 122 71 631 605 2715 1525
Fragments arranged by size:
2715 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5771 5771
Size: 5776
BlpI GC'TnA_GC
Cuts at: 3193 3193
Size: 5776
Bme1580I G_kGCm'C
Cuts at: 1790 2200 3837 4993 1790
Size: 410 1637 1156 2573
Fragments arranged by size:
2573 1637 1156 410
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5776
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3507 4465 442
Size: 416 53 83 399 24 2090 958 1753
Fragments arranged by size:
2090 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4436 1239
Size: 3197 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3901 4766 5064 3901
Size: 865 298 4613
Fragments arranged by size:
4613 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2258 4439 882
Size: 617 759 2181 2219
Fragments arranged by size:
2219 2181 759 617
BsaAI yAC'GTr
Cuts at: 493 1204 3567 493
Size: 711 2363 2702
Fragments arranged by size:
2702 2363 711
BsaBI GATnn'nnATC
Cuts at: 5770 5770
Size: 5776
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3583 3965 4600 275
Size: 53 83 186 936 2050 382 635 1451
Fragments arranged by size:
2050 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2181 4209 4607 4881 5028 5439 2181
Size: 2028 398 274 147 411 2518
Fragments arranged by size:
2518 2028 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2969 2999 1178
Size: 30 165 30 1566 30 3955
Fragments arranged by size:
3955 1566 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5776
BseYI C'CCAG_C
Cuts at: 2201 4979 5475 2201
Size: 2778 496 2502
Fragments arranged by size:
2778 2502 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5203
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3226 3987 5015 1540
Size: 275 186 1225 761 1028 2301
Fragments arranged by size:
2301 1225 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2489 3837 3922 4993 2489
Size: 1348 85 1071 3272
Fragments arranged by size:
3272 1348 1071 85
BsmI GAATG_Cn'
Cuts at: 2283 3287 3380 2283
Size: 1004 93 4679
Fragments arranged by size:
4679 1004 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2200 2489 3095 3837 3922 4993
Size: 399 352 58 289 606 742 85 1071
Cuts at: 4993 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 606 399 352 289 85
58
BspEI T'CCGG_A
Cuts at: 5439 5439
Size: 5776
BspHI T'CATG_A
Cuts at: 3665 5395 3665
Size: 1730 4046
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2874 3131 844
Size: 2030 257 3489
Fragments arranged by size:
3489 2030 257
BsrBI CCG'CTC
Cuts at: 1394 1823 3663 1394
Size: 429 1840 3507
Fragments arranged by size:
3507 1840 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4271 4445 65
Size: 1183 3023 174 1396
Fragments arranged by size:
3023 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4420 4607 1700
Size: 110 2610 187 2869
Fragments arranged by size:
2869 2610 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5776
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5776
BssSI C'ACGA_G
Cuts at: 3836 4848 3836
Size: 1012 4764
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5776
BstBI TT'CG_AA
Cuts at: 2386 4580 2386
Size: 2194 3582
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5776
BstYI r'GATC_y
Cuts at: 1972 2580 3106 3473 3858 3875 5327 5413 5425
Size: 608 526 367 385 17 1452 86 12
Cuts at: 5425 5771 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 608 526 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2121 2490 513
Size: 552 939 117 369 3799
Fragments arranged by size:
3799 939 552 369 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5037
Fragments arranged by size:
5037 683 56
BtsI GCAGTG_nn'
Cuts at: 2615 3292 4086 4106 2615
Size: 677 794 20 4285
Fragments arranged by size:
4285 794 677 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5776
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5741
DraI TTT'AAA
Cuts at: 2958 3080 2958
Size: 122 5654
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4783 809
Size: 507 3467 1802
Fragments arranged by size:
3467 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2041 2323 2944
Size: 54 1425 50 275 18 211 282 621
Cuts at: 2944 3223 4112 5741 8
Size: 279 889 1629 43
Fragments arranged by size:
1629 1425 889 621 282 279 275 211
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3223 1537
Size: 275 1411 4090
Fragments arranged by size:
4090 1411 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2094 2571 3706 1353
Size: 741 477 1135 3423
Fragments arranged by size:
3423 1135 741 477
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4363 4737 4883 387
Size: 1064 138 2774 374 146 1280
Fragments arranged by size:
2774 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2898 3839 4436 5223 5491 1239
Size: 393 1266 941 597 787 268 1524
Fragments arranged by size:
1524 1266 941 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2196 2197 2196
Size: 1 5775
EcoRI G'AATT_C
Cuts at: 2621 2998 3085 3463 2621
Size: 377 87 378 4934
Fragments arranged by size:
4934 378 377 87
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2416 2416
Size: 5776
FspI TGC'GCA
Cuts at: 4282 4282
Size: 5776
HaeII r_GCGC'y
Cuts at: 1678 4923 1678
Size: 3245 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4417 4449 4491 4523 1373
Size: 32 135 32 2845 32 42 32 2626
Fragments arranged by size:
2845 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2506 3120 3366 669
Size: 988 281 568 614 246 3079
Fragments arranged by size:
3079 988 614 568 281 246
HindIII A'AGCT_T
Cuts at: 748 2534 748
Size: 1786 3990
HpaI GTT'AAC
Cuts at: 3366 3366
Size: 5776
KpnI G_GTAC'C
Cuts at: 3101 3101
Size: 5776
MfeI C'AATT_G
Cuts at: 3375 3375
Size: 5776
MluI A'CGCG_T
Cuts at: 5652 5652
Size: 5776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2206 2769 4890 5074 1305
Size: 901 563 2121 184 2007
Fragments arranged by size:
2121 2007 901 563 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2325 10
Size: 54 1425 343 493 3461
Fragments arranged by size:
3461 1425 493 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2334 3734 4093 4252 518
Size: 648 1168 1400 359 159 2042
Fragments arranged by size:
2042 1400 1168 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2903 3192 3869 5017 5262 1606
Size: 1297 289 677 1148 245 2120
Fragments arranged by size:
2120 1297 1148 677 289 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2121 2490 513
Size: 552 939 117 369 3799
Fragments arranged by size:
3799 939 552 369 117
NdeI CA'TA_TG
Cuts at: 387 2730 387
Size: 2343 3433
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5776
NotI GC'GGCC_GC
Cuts at: 3223 3223
Size: 5776
NspI r_CATG'y
Cuts at: 1132 1229 2595 2837 3134 4679 1132
Size: 97 1366 242 297 1545 2229
Fragments arranged by size:
2229 1545 1366 297 242 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5776
PciI A'CATG_T
Cuts at: 2591 4675 2591
Size: 2084 3692
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5776
PfoI T'CCnGG_A
Cuts at: 2463 2463
Size: 5776
PmeI GTTT'AAAC
Cuts at: 3080 3080
Size: 5776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3845 3877 5358 5390 3845
Size: 32 1481 32 4231
Fragments arranged by size:
4231 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5776
PsiI TTA'TAA
Cuts at: 2030 3346 2030
Size: 1316 4460
PspOMI G'GGCC_C
Cuts at: 2196 2196
Size: 5776
PstI C_TGCA'G
Cuts at: 830 3128 830
Size: 2298 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5776
SalI G'TCGA_C
Cuts at: 1936 3118 1936
Size: 1182 4594
SbfI CC_TGCA'GG
Cuts at: 3128 3128
Size: 5776
ScaI AGT'ACT
Cuts at: 2475 4024 5736 2475
Size: 1549 1712 2515
Fragments arranged by size:
2515 1712 1549
SfcI C'TryA_G
Cuts at: 826 1045 2603 2838 3124 4259 4940 5131 826
Size: 219 1558 235 286 1135 681 191 1471
Fragments arranged by size:
1558 1471 1135 681 286 235 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5776
SmaI CCC'GGG
Cuts at: 1872 3103 1872
Size: 1231 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3880 4781 5043 820
Size: 197 926 1937 901 262 1553
Fragments arranged by size:
1937 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3567 493
Size: 3074 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5776
SphI G_CATG'C
Cuts at: 1132 2837 3134 1132
Size: 1705 297 3774
Fragments arranged by size:
3774 1705 297
SspI AAT'ATT
Cuts at: 5 52 2953 3700 5
Size: 47 2901 747 2081
Fragments arranged by size:
2901 2081 747 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5776
StyI C'CwwG_G
Cuts at: 513 1065 2004 2121 2490 3215 513
Size: 552 939 117 369 725 3074
Fragments arranged by size:
3074 939 725 552 369 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3803 3820 3973 4158 4603 325
Size: 1255 2223 17 153 185 445 1498
Fragments arranged by size:
2223 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2473 4022 5734
Size: 274 80 33 51 1443 496 1549 1712
Cuts at: 5734 96
Size: 138
Fragments arranged by size:
1712 1549 1443 496 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2163 2195 2790 2822 5736 5768 2163
Size: 32 595 32 2914 32 2171
Fragments arranged by size:
2914 2171 595 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5776
XbaI T'CTAG_A
Cuts at: 3112 3112
Size: 5776
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2333 2735 2333
Size: 402 5374
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5776
XmaI C'CCGG_G
Cuts at: 1870 3101 1870
Size: 1231 4545
XmnI GAAnn'nnTTC
Cuts at: 2784 3905 2784
Size: 1121 4655
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3584 276
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI