Restriction map for
pFN21AE1837
(Circular) (Six-base) MAPSORT of: pFN21AE1837.seq Check: 8957 from: 1 to: 5767
pFN21AE1837 5767 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2678 3021 3032 2678
Size: 343 11 5413
Fragments arranged by size:
5413 343 11
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3577 278
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2575 3110 1937
Size: 638 535 4594
Fragments arranged by size:
4594 638 535
Acc65I G'GTAC_C
Cuts at: 3088 3088
Size: 5767
AclI AA'CG_TT
Cuts at: 1563 3894 4267 1563
Size: 2331 373 3063
Fragments arranged by size:
3063 2331 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3830 5214 5482 1632
Size: 2198 1384 268 1917
Fragments arranged by size:
2198 1917 1384 268
AfeI AGC'GCT
Cuts at: 3053 3053
Size: 5767
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5570
AflIII A'CryG_T
Cuts at: 1203 4666 5643 1203
Size: 3463 977 1327
Fragments arranged by size:
3463 1327 977
AgeI A'CCGG_T
Cuts at: 4598 4598
Size: 5767
AhdI GACnn_n'nnGTC
Cuts at: 4496 4496
Size: 5767
AleI CACnn'nnGTG
Cuts at: 1166 2210 2583 1166
Size: 1044 373 4350
Fragments arranged by size:
4350 1044 373
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5767
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2264 2296 2264
Size: 32 5735
AlwNI CAG_nnn'CTG
Cuts at: 2605 2692 3510 5082 2605
Size: 87 818 1572 3290
Fragments arranged by size:
3290 1572 818 87
ApaI G_GGCC'C
Cuts at: 2454 2454
Size: 5767
ApaLI G'TGCA_C
Cuts at: 2213 2747 3824 4980 2213
Size: 534 1077 1156 3000
Fragments arranged by size:
3000 1156 1077 534
ApoI r'AATT_y
Cuts at: 1493 3076 3302 3454 5563 1493
Size: 1583 226 152 2109 1697
Fragments arranged by size:
2109 1697 1583 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4321 160
Size: 872 3289 1606
Fragments arranged by size:
3289 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
AvaI C'yCGr_G
Cuts at: 1870 1943 2328 2385 2447 2453 3092 1870
Size: 73 385 57 62 6 639 4545
Fragments arranged by size:
4545 639 385 73 62 57 6
BamHI G'GATC_C
Cuts at: 2549 3097 3464 2549
Size: 548 367 4852
Fragments arranged by size:
4852 548 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2762 2779 2988 3088 4543
Size: 325 217 627 975 17 209 100 1455
Cuts at: 4543 618
Size: 1842
Fragments arranged by size:
1842 1455 975 627 325 217 209 100
17
BanII G_rGCy'C
Cuts at: 1391 2199 2292 2454 2499 2840 2876 3086 1391
Size: 808 93 162 45 341 36 210 4072
Fragments arranged by size:
4072 808 341 210 162 93 45 36
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4796
BbvCI CC'TCA_GC
Cuts at: 2486 2486
Size: 5767
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3958 3992 1903
Size: 34 2021 34 3678
Fragments arranged by size:
3678 2021 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3659 4869 3659
Size: 1210 4557
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5767
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2449 4378
Size: 107 122 71 631 605 337 440 1929
Cuts at: 4378 136
Size: 1525
Fragments arranged by size:
1929 1525 631 605 440 337 122 107
71
BglII A'GATC_T
Cuts at: 5762 5762
Size: 5767
BlpI GC'TnA_GC
Cuts at: 3184 3184
Size: 5767
Bme1580I G_kGCm'C
Cuts at: 1790 2217 2454 2751 2765 2784 3828 4984 1790
Size: 427 237 297 14 19 1044 1156 2573
Fragments arranged by size:
2573 1156 1044 427 297 237 19 14
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5767
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3498 4456 442
Size: 416 53 83 399 24 2081 958 1753
Fragments arranged by size:
2081 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5767
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2619 2619
Size: 5767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2039 2168 2213 2336 4427 1239
Size: 800 129 45 123 2091 2579
Fragments arranged by size:
2579 2091 800 129 123 45
Bpu10I CC'TnA_GC
Cuts at: 2486 2486
Size: 5767
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2699 3892 4757 5055 2699
Size: 1193 865 298 3411
Fragments arranged by size:
3411 1193 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4430 882
Size: 617 2931 2219
Fragments arranged by size:
2931 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3558 493
Size: 711 2354 2702
Fragments arranged by size:
2702 2354 711
BsaBI GATnn'nnATC
Cuts at: 5761 5761
Size: 5767
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3574 3956 4591 275
Size: 53 83 186 936 2041 382 635 1451
Fragments arranged by size:
2041 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4200 4598 4872 5019 5430 4200
Size: 398 274 147 411 4537
Fragments arranged by size:
4537 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2264 2294 1178
Size: 30 165 30 861 30 4651
Fragments arranged by size:
4651 861 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2269 2489 1186
Size: 1083 220 4464
Fragments arranged by size:
4464 1083 220
BseYI C'CCAG_C
Cuts at: 2463 4970 5466 2463
Size: 2507 496 2764
Fragments arranged by size:
2764 2507 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2148 2192 2377 1163
Size: 573 412 44 185 4553
Fragments arranged by size:
4553 573 412 185 44
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2018 2361 3217 3978 5006 1540
Size: 275 186 17 343 856 761 1028 2301
Fragments arranged by size:
2301 1028 856 761 343 275 186 17
BsiHKAI G_wGCw'C
Cuts at: 2199 2217 2292 2751 2840 3828 3913 4984 2199
Size: 18 75 459 89 988 85 1071 2982
Fragments arranged by size:
2982 1071 988 459 89 85 75 18
BsmI GAATG_Cn'
Cuts at: 3278 3371 3278
Size: 93 5674
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2199 2217 2292 2454 2499 2751 2765
Size: 399 409 18 75 162 45 252 14
Cuts at: 2765 2784 2840 2876 3086 3828 3913 4984 1391
Size: 19 56 36 210 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 409 399 252 210 162
85 75 56 45 36 19 18 14
BspEI T'CCGG_A
Cuts at: 5430 5430
Size: 5767
BspHI T'CATG_A
Cuts at: 2232 3656 5386 2232
Size: 1424 1730 2613
Fragments arranged by size:
2613 1730 1424
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2011 2415 2678 3021 3032 3122 844
Size: 1167 404 263 343 11 90 3489
Fragments arranged by size:
3489 1167 404 343 263 90 11
BsrBI CCG'CTC
Cuts at: 1394 1823 2986 3654 1394
Size: 429 1163 668 3507
Fragments arranged by size:
3507 1163 668 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4262 4436 65
Size: 1183 3014 174 1396
Fragments arranged by size:
3014 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2060 4411 4598 1700
Size: 110 250 2351 187 2869
Fragments arranged by size:
2869 2351 250 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5767
BssHII G'CGCG_C
Cuts at: 1925 2608 1925
Size: 683 5084
BssSI C'ACGA_G
Cuts at: 3827 4839 3827
Size: 1012 4755
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2692 2779 794
Size: 1898 87 3782
Fragments arranged by size:
3782 1898 87
BstBI TT'CG_AA
Cuts at: 4571 4571
Size: 5767
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5767
BstXI CCAn_nnnn'nTGG
Cuts at: 2791 2791
Size: 5767
BstYI r'GATC_y
Cuts at: 1972 2549 3097 3464 3849 3866 5318 5404 5416
Size: 577 548 367 385 17 1452 86 12
Cuts at: 5416 5762 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 577 548 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2638 2757 2878 3045 513
Size: 552 1573 119 121 167 3235
Fragments arranged by size:
3235 1573 552 167 121 119
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2365 487
Size: 683 56 1139 3889
Fragments arranged by size:
3889 1139 683 56
BtsI GCAGTG_nn'
Cuts at: 2134 2903 3283 4077 4097 2134
Size: 769 380 794 20 3804
Fragments arranged by size:
3804 794 769 380 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5767
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5732
DraI TTT'AAA
Cuts at: 3071 3071
Size: 5767
DraIII CAC_nnn'GTG
Cuts at: 2209 2779 2209
Size: 570 5197
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2332 2978 4774 809
Size: 507 1016 646 1796 1802
Fragments arranged by size:
1802 1796 1016 646 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2015 2358 2733
Size: 54 1425 50 275 18 185 343 375
Cuts at: 2733 3214 4103 5732 8
Size: 481 889 1629 43
Fragments arranged by size:
1629 1425 889 481 375 343 275 185
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2015 2358 3214 1537
Size: 275 203 343 856 4090
Fragments arranged by size:
4090 856 343 275 203
EarI CTCTTCn'nnn_
Cuts at: 1353 2045 3697 1353
Size: 692 1652 3423
Fragments arranged by size:
3423 1652 692
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4354 4728 4874 387
Size: 1064 138 2765 374 146 1280
Fragments arranged by size:
2765 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2197 2290 2838 2197
Size: 93 548 5126
Fragments arranged by size:
5126 548 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2168 2213 2336 3830 4427 5214
Size: 393 407 129 45 123 1494 597 787
Cuts at: 5214 5482 1239
Size: 268 1524
Fragments arranged by size:
1524 1494 787 597 407 393 268 129
123 45
EcoNI CCTnn'n_nnAGG
Cuts at: 3028 3028
Size: 5767
EcoO109I rG'GnC_Cy
Cuts at: 2325 2788 2325
Size: 463 5304
EcoRI G'AATT_C
Cuts at: 3076 3454 3076
Size: 378 5389
EcoRV GAT'ATC
Cuts at: 2797 2797
Size: 5767
FspI TGC'GCA
Cuts at: 4273 4273
Size: 5767
HaeII r_GCGC'y
Cuts at: 1678 2036 2054 2093 3055 4914 1678
Size: 358 18 39 962 1859 2531
Fragments arranged by size:
2531 1859 962 358 39 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4408 4440 4482 4514 1373
Size: 32 135 32 2836 32 42 32 2626
Fragments arranged by size:
2836 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3111 3357 669
Size: 988 281 1173 246 3079
Fragments arranged by size:
3079 1173 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5767
HpaI GTT'AAC
Cuts at: 3357 3357
Size: 5767
KpnI G_GTAC'C
Cuts at: 3092 3092
Size: 5767
MfeI C'AATT_G
Cuts at: 3366 3366
Size: 5767
MluI A'CGCG_T
Cuts at: 5643 5643
Size: 5767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2937 4881 5065 1305
Size: 1632 1944 184 2007
Fragments arranged by size:
2007 1944 1632 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3945
Fragments arranged by size:
3945 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2210 2583 3725 4084 4243 518
Size: 648 1044 373 1142 359 159 2042
Fragments arranged by size:
2042 1142 1044 648 373 359 159
MspA1I CmG'CkG
Cuts at: 1606 2146 2176 2463 2928 3183 3860 5008 5253
Size: 540 30 287 465 255 677 1148 245
Cuts at: 5253 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 540 465 287 255 245
30
NcoI C'CATG_G
Cuts at: 513 1065 2638 2757 513
Size: 552 1573 119 3523
Fragments arranged by size:
3523 1573 552 119
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5767
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5767
NotI GC'GGCC_GC
Cuts at: 2015 3214 2015
Size: 1199 4568
NspI r_CATG'y
Cuts at: 1132 1229 2266 3125 4670 1132
Size: 97 1037 859 1545 2229
Fragments arranged by size:
2229 1545 1037 859 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5767
PciI A'CATG_T
Cuts at: 4666 4666
Size: 5767
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5767
PmeI GTTT'AAAC
Cuts at: 3071 3071
Size: 5767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2264 2296 3836 3868 5349 5381 2264
Size: 32 1540 32 1481 32 2650
Fragments arranged by size:
2650 1540 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2325 2788 2325
Size: 463 5304
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5767
PsiI TTA'TAA
Cuts at: 3337 3337
Size: 5767
PspOMI G'GGCC_C
Cuts at: 2450 2450
Size: 5767
PstI C_TGCA'G
Cuts at: 830 2688 2697 2835 3119 830
Size: 1858 9 138 284 3478
Fragments arranged by size:
3478 1858 284 138 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5767
PvuII CAG'CTG
Cuts at: 2146 2176 2928 2146
Size: 30 752 4985
Fragments arranged by size:
4985 752 30
SacI G_AGCT'C
Cuts at: 2199 2292 2840 2199
Size: 93 548 5126
Fragments arranged by size:
5126 548 93
SalI G'TCGA_C
Cuts at: 1936 3109 1936
Size: 1173 4594
SanDI GG'GwC_CC
Cuts at: 2325 2325
Size: 5767
SapI GCTCTTCn'nnn_
Cuts at: 2045 2045
Size: 5767
SbfI CC_TGCA'GG
Cuts at: 3119 3119
Size: 5767
ScaI AGT'ACT
Cuts at: 4015 5727 4015
Size: 1712 4055
SexAI A'CCwGG_T
Cuts at: 2775 2775
Size: 5767
SfcI C'TryA_G
Cuts at: 826 1045 2684 2693 2831 3115 4250 4931 5122
Size: 219 1639 9 138 284 1135 681 191
Cuts at: 5122 826
Size: 1471
Fragments arranged by size:
1639 1471 1135 681 284 219 191 138
9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5767
SmaI CCC'GGG
Cuts at: 1872 2330 2387 2449 2455 3094 1872
Size: 458 57 62 6 639 4545
Fragments arranged by size:
4545 639 458 62 57 6
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2714 3871 4772 5034 820
Size: 197 926 771 1157 901 262 1553
Fragments arranged by size:
1553 1157 926 901 771 262 197
SnaBI TAC'GTA
Cuts at: 493 3558 493
Size: 3065 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5767
SphI G_CATG'C
Cuts at: 1132 3125 1132
Size: 1993 3774
SrfI GCCC'GGGC
Cuts at: 2387 2387
Size: 5767
SspI AAT'ATT
Cuts at: 5 52 3691 5
Size: 47 3639 2081
Fragments arranged by size:
3639 2081 47
StuI AGG'CCT
Cuts at: 1517 2240 1517
Size: 723 5044
StyI C'CwwG_G
Cuts at: 513 1065 2638 2739 2757 2784 3206 513
Size: 552 1573 101 18 27 422 3074
Fragments arranged by size:
3074 1573 552 422 101 27 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3794 3811 3964 4149 4594 325
Size: 1255 2214 17 153 185 445 1498
Fragments arranged by size:
2214 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4013 5725 96
Size: 274 80 33 51 1443 2036 1712 138
Fragments arranged by size:
2036 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2612 2644 5727 5759 2612
Size: 32 3083 32 2620
Fragments arranged by size:
3083 2620 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5767
XbaI T'CTAG_A
Cuts at: 3103 3103
Size: 5767
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2645 2645
Size: 5767
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5767
XmaI C'CCGG_G
Cuts at: 1870 2328 2385 2447 2453 3092 1870
Size: 458 57 62 6 639 4545
Fragments arranged by size:
4545 639 458 62 57 6
XmnI GAAnn'nnTTC
Cuts at: 3896 3896
Size: 5767
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3575 276
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I
Bsu36I FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SacII SfiI SfoI SwaI