Restriction map for
pFN21AE1836
(Circular) (Six-base) MAPSORT of: pFN21AE1836.seq Check: 8060 from: 1 to: 5767
pFN21AE1836 5767 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2945 2945
Size: 5767
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3577 278
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3110 1937
Size: 1173 4594
Acc65I G'GTAC_C
Cuts at: 3088 3088
Size: 5767
AclI AA'CG_TT
Cuts at: 1563 3894 4267 1563
Size: 2331 373 3063
Fragments arranged by size:
3063 2331 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2655 3830 5214 5482 1632
Size: 1023 1175 1384 268 1917
Fragments arranged by size:
1917 1384 1175 1023 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5570
AflIII A'CryG_T
Cuts at: 1203 2418 4666 5643 1203
Size: 1215 2248 977 1327
Fragments arranged by size:
2248 1327 1215 977
AgeI A'CCGG_T
Cuts at: 4598 4598
Size: 5767
AhdI GACnn_n'nnGTC
Cuts at: 4496 4496
Size: 5767
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5767
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3025 1184
Size: 1841 3926
AlwNI CAG_nnn'CTG
Cuts at: 2531 3510 5082 2531
Size: 979 1572 3216
Fragments arranged by size:
3216 1572 979
ApaI G_GGCC'C
Cuts at: 3048 3048
Size: 5767
ApaLI G'TGCA_C
Cuts at: 3824 4980 3824
Size: 1156 4611
ApoI r'AATT_y
Cuts at: 1493 3076 3302 3454 5563 1493
Size: 1583 226 152 2109 1697
Fragments arranged by size:
2109 1697 1583 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4321 160
Size: 872 3289 1606
Fragments arranged by size:
3289 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
AvaI C'yCGr_G
Cuts at: 1870 1943 3092 1870
Size: 73 1149 4545
Fragments arranged by size:
4545 1149 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2511 2544 2511
Size: 33 5734
BamHI G'GATC_C
Cuts at: 3097 3464 3097
Size: 367 5400
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3088 4543 618
Size: 325 217 627 1301 1455 1842
Fragments arranged by size:
1842 1455 1301 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2069 3048 3086 1391
Size: 678 979 38 4072
Fragments arranged by size:
4072 979 678 38
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2751 928
Size: 971 852 3944
Fragments arranged by size:
3944 971 852
BbvCI CC'TCA_GC
Cuts at: 2210 2210
Size: 5767
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3958 3992 1903
Size: 34 2021 34 3678
Fragments arranged by size:
3678 2021 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3659 4869 3659
Size: 1210 4557
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5767
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4378 136
Size: 107 122 71 631 605 2706 1525
Fragments arranged by size:
2706 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2842 5762 2842
Size: 2920 2847
BlpI GC'TnA_GC
Cuts at: 2245 3184 2245
Size: 939 4828
Bme1580I G_kGCm'C
Cuts at: 1790 3048 3828 4984 1790
Size: 1258 780 1156 2573
Fragments arranged by size:
2573 1258 1156 780
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5767
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3498 4456 442
Size: 416 53 83 399 24 2081 958 1753
Fragments arranged by size:
2081 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2053 2744 2915 4427 1239
Size: 814 691 171 1512 2579
Fragments arranged by size:
2579 1512 814 691 171
Bpu10I CC'TnA_GC
Cuts at: 2210 2210
Size: 5767
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3892 4757 5055 3892
Size: 865 298 4604
Fragments arranged by size:
4604 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2891 4430 882
Size: 617 1392 1539 2219
Fragments arranged by size:
2219 1539 1392 617
BsaAI yAC'GTr
Cuts at: 493 1204 3558 493
Size: 711 2354 2702
Fragments arranged by size:
2702 2354 711
BsaBI GATnn'nnATC
Cuts at: 5761 5761
Size: 5767
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3574 3956 4591 275
Size: 53 83 186 936 2041 382 635 1451
Fragments arranged by size:
2041 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4200 4598 4872 5019 5430 4200
Size: 398 274 147 411 4537
Fragments arranged by size:
4537 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5542
Fragments arranged by size:
5542 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5767
BseYI C'CCAG_C
Cuts at: 2374 4970 5466 2374
Size: 2596 496 2675
Fragments arranged by size:
2675 2596 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2081 2356 1163
Size: 573 345 275 4574
Fragments arranged by size:
4574 573 345 275
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3217 3978 5006 1540
Size: 275 186 1216 761 1028 2301
Fragments arranged by size:
2301 1216 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3828 3913 4984 3828
Size: 85 1071 4611
Fragments arranged by size:
4611 1071 85
BsmI GAATG_Cn'
Cuts at: 3278 3371 3278
Size: 93 5674
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2069 3048 3086 3828 3913 4984 1391
Size: 399 279 979 38 742 85 1071 2174
Fragments arranged by size:
2174 1071 979 742 399 279 85 38
BspEI T'CCGG_A
Cuts at: 5430 5430
Size: 5767
BspHI T'CATG_A
Cuts at: 2037 3656 5386 2037
Size: 1619 1730 2418
Fragments arranged by size:
2418 1730 1619
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2909 2945 3122 844
Size: 2065 36 177 3489
Fragments arranged by size:
3489 2065 177 36
BsrBI CCG'CTC
Cuts at: 1394 1823 3654 1394
Size: 429 1831 3507
Fragments arranged by size:
3507 1831 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2449 3007 4262 4436 65
Size: 1183 1201 558 1255 174 1396
Fragments arranged by size:
1396 1255 1201 1183 558 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4411 4598 1700
Size: 110 2601 187 2869
Fragments arranged by size:
2869 2601 187 110
BsrGI T'GTAC_A
Cuts at: 96 2007 96
Size: 1911 3856
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5767
BssSI C'ACGA_G
Cuts at: 3827 4839 3827
Size: 1012 4755
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5767
BstBI TT'CG_AA
Cuts at: 2167 4571 2167
Size: 2404 3363
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5767
BstXI CCAn_nnnn'nTGG
Cuts at: 2374 2374
Size: 5767
BstYI r'GATC_y
Cuts at: 1972 2618 2628 2842 3097 3464 3849 3866 5318
Size: 646 10 214 255 367 385 17 1452
Cuts at: 5318 5404 5416 5762 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 646 385 367 346 255 214
86 17 12 10
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5215
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5028
Fragments arranged by size:
5028 683 56
BtsI GCAGTG_nn'
Cuts at: 3283 4077 4097 3283
Size: 794 20 4953
Fragments arranged by size:
4953 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5767
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2251 2286 529
Size: 35 1687 35 4010
Fragments arranged by size:
4010 1687 35 35
DraI TTT'AAA
Cuts at: 3071 3071
Size: 5767
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4774 809
Size: 507 3458 1802
Fragments arranged by size:
3458 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2364 3214 4103
Size: 54 1425 50 275 18 534 850 889
Cuts at: 4103 5732 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 850 534 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3214 1537
Size: 275 1402 4090
Fragments arranged by size:
4090 1402 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2375 2442 3697 1353
Size: 1022 67 1255 3423
Fragments arranged by size:
3423 1255 1022 67
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4354 4728 4874 387
Size: 1064 138 2765 374 146 1280
Fragments arranged by size:
2765 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2053 2655 2744 2915 3830 4427 5214
Size: 393 421 602 89 171 915 597 787
Cuts at: 5214 5482 1239
Size: 268 1524
Fragments arranged by size:
1524 915 787 602 597 421 393 268
171 89
EcoO109I rG'GnC_Cy
Cuts at: 3044 3045 3044
Size: 1 5766
EcoRI G'AATT_C
Cuts at: 3076 3454 3076
Size: 378 5389
EcoRV GAT'ATC
Cuts at: 2508 2508
Size: 5767
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2471 2471
Size: 5767
FspI TGC'GCA
Cuts at: 4273 4273
Size: 5767
HaeII r_GCGC'y
Cuts at: 1678 4914 1678
Size: 3236 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2228 2260 4408 4440 4482
Size: 32 135 32 656 32 2148 32 42
Cuts at: 4482 4514 1373
Size: 32 2626
Fragments arranged by size:
2626 2148 656 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3111 3357 669
Size: 988 281 1173 246 3079
Fragments arranged by size:
3079 1173 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5767
HpaI GTT'AAC
Cuts at: 3357 3357
Size: 5767
KpnI G_GTAC'C
Cuts at: 3092 3092
Size: 5767
MfeI C'AATT_G
Cuts at: 3366 3366
Size: 5767
MluI A'CGCG_T
Cuts at: 5643 5643
Size: 5767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4881 5065 1305
Size: 3576 184 2007
Fragments arranged by size:
3576 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2366 10
Size: 54 1425 343 534 3411
Fragments arranged by size:
3411 1425 534 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3725 4084 4243 518
Size: 648 2559 359 159 2042
Fragments arranged by size:
2559 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2056 2943 3183 3860 5008 5253 1606
Size: 450 887 240 677 1148 245 2120
Fragments arranged by size:
2120 1148 887 677 450 245 240
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5215
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5767
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5767
NotI GC'GGCC_GC
Cuts at: 3214 3214
Size: 5767
NspI r_CATG'y
Cuts at: 1132 1229 2683 3000 3125 4670 1132
Size: 97 1454 317 125 1545 2229
Fragments arranged by size:
2229 1545 1454 317 125 97
PasI CC'CwG_GG
Cuts at: 1348 3040 1348
Size: 1692 4075
PciI A'CATG_T
Cuts at: 4666 4666
Size: 5767
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2735 1108
Size: 1627 4140
PfoI T'CCnGG_A
Cuts at: 2760 2760
Size: 5767
PmeI GTTT'AAAC
Cuts at: 3071 3071
Size: 5767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2757 2789 3836 3868 5349 5381 2757
Size: 32 1047 32 1481 32 3143
Fragments arranged by size:
3143 1481 1047 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5767
PsiI TTA'TAA
Cuts at: 3337 3337
Size: 5767
PspOMI G'GGCC_C
Cuts at: 3044 3044
Size: 5767
PstI C_TGCA'G
Cuts at: 830 2870 2942 3119 830
Size: 2040 72 177 3478
Fragments arranged by size:
3478 2040 177 72
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5767
PvuII CAG'CTG
Cuts at: 2056 2943 2056
Size: 887 4880
SalI G'TCGA_C
Cuts at: 1936 3109 1936
Size: 1173 4594
SbfI CC_TGCA'GG
Cuts at: 3119 3119
Size: 5767
ScaI AGT'ACT
Cuts at: 4015 5727 4015
Size: 1712 4055
SfcI C'TryA_G
Cuts at: 826 1045 2866 2938 3115 4250 4931 5122 826
Size: 219 1821 72 177 1135 681 191 1471
Fragments arranged by size:
1821 1471 1135 681 219 191 177 72
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5767
SmaI CCC'GGG
Cuts at: 1872 3094 1872
Size: 1222 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3871 4772 5034 820
Size: 197 926 1928 901 262 1553
Fragments arranged by size:
1928 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3558 493
Size: 3065 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5767
SphI G_CATG'C
Cuts at: 1132 3125 1132
Size: 1993 3774
SspI AAT'ATT
Cuts at: 5 52 3691 5
Size: 47 3639 2081
Fragments arranged by size:
3639 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5767
StyI C'CwwG_G
Cuts at: 513 1065 3206 513
Size: 552 2141 3074
Fragments arranged by size:
3074 2141 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2247 2943 3794 3811 3964 4149 4594
Size: 1255 667 696 851 17 153 185 445
Cuts at: 4594 325
Size: 1498
Fragments arranged by size:
1498 1255 851 696 667 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2007 4013 5725
Size: 274 80 33 51 1443 30 2006 1712
Cuts at: 5725 96
Size: 138
Fragments arranged by size:
2006 1712 1443 274 138 80 51 33
30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5727 5759 5727
Size: 32 5735
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5767
XbaI T'CTAG_A
Cuts at: 3103 3103
Size: 5767
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5767
XmaI C'CCGG_G
Cuts at: 1870 3092 1870
Size: 1222 4545
XmnI GAAnn'nnTTC
Cuts at: 2033 2163 2420 3896 2033
Size: 130 257 1476 3904
Fragments arranged by size:
3904 1476 257 130
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3575 276
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI