Restriction map for
pFN21AE1829
(Circular) (Six-base) MAPSORT of: pFN21AE1829.seq Check: 9392 from: 1 to: 5746
pFN21AE1829 5746 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3556 278
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2713 3089 1937
Size: 776 376 4594
Fragments arranged by size:
4594 776 376
Acc65I G'GTAC_C
Cuts at: 3067 3067
Size: 5746
AclI AA'CG_TT
Cuts at: 1563 2908 3873 4246 1563
Size: 1345 965 373 3063
Fragments arranged by size:
3063 1345 965 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2361 2513 3809 5193 5461 1632
Size: 729 152 1296 1384 268 1917
Fragments arranged by size:
1917 1384 1296 729 268 152
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5549
AflIII A'CryG_T
Cuts at: 1203 4645 5622 1203
Size: 3442 977 1327
Fragments arranged by size:
3442 1327 977
AgeI A'CCGG_T
Cuts at: 4577 4577
Size: 5746
AhdI GACnn_n'nnGTC
Cuts at: 4475 4475
Size: 5746
AleI CACnn'nnGTG
Cuts at: 1166 2576 1166
Size: 1410 4336
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5746
AlwNI CAG_nnn'CTG
Cuts at: 2899 3489 5061 2899
Size: 590 1572 3584
Fragments arranged by size:
3584 1572 590
ApaI G_GGCC'C
Cuts at: 2050 2509 2671 2050
Size: 459 162 5125
Fragments arranged by size:
5125 459 162
ApaLI G'TGCA_C
Cuts at: 3803 4959 3803
Size: 1156 4590
ApoI r'AATT_y
Cuts at: 1493 3055 3281 3433 5542 1493
Size: 1562 226 152 2109 1697
Fragments arranged by size:
2109 1697 1562 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4300 160
Size: 872 3268 1606
Fragments arranged by size:
3268 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
AvaI C'yCGr_G
Cuts at: 1870 1943 3071 1870
Size: 73 1128 4545
Fragments arranged by size:
4545 1128 73
BamHI G'GATC_C
Cuts at: 3076 3443 3076
Size: 367 5379
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2706 3039 3067 4522 618
Size: 325 217 627 919 333 28 1455 1842
Fragments arranged by size:
1842 1455 919 627 333 325 217 28
BanII G_rGCy'C
Cuts at: 1391 2050 2283 2327 2509 2660 2671 2792 3065
Size: 659 233 44 182 151 11 121 273
Cuts at: 3065 1391
Size: 4072
Fragments arranged by size:
4072 659 273 233 182 151 121 44
11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2960 928
Size: 971 1061 3714
Fragments arranged by size:
3714 1061 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3937 3971 1903
Size: 34 50 34 1916 34 3678
Fragments arranged by size:
3678 1916 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3638 4848 3638
Size: 1210 4536
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5746
BfrBI ATG'CAT
Cuts at: 2885 2885
Size: 5746
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2045 4357 136
Size: 107 122 71 631 605 373 2312 1525
Fragments arranged by size:
2312 1525 631 605 373 122 107 71
BglII A'GATC_T
Cuts at: 5741 5741
Size: 5746
BlpI GC'TnA_GC
Cuts at: 2138 2277 2966 3163 2138
Size: 139 689 197 4721
Fragments arranged by size:
4721 689 197 139
Bme1580I G_kGCm'C
Cuts at: 1790 2050 2509 2671 2947 3807 4963 1790
Size: 260 459 162 276 860 1156 2573
Fragments arranged by size:
2573 1156 860 459 276 260 162
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5746
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2942 3477 4435
Size: 416 53 83 399 24 1525 535 958
Cuts at: 4435 442
Size: 1753
Fragments arranged by size:
1753 1525 958 535 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2039 2531 2556 4406 1239
Size: 800 492 25 1850 2579
Fragments arranged by size:
2579 1850 800 492 25
Bpu10I CC'TnA_GC
Cuts at: 2249 2249
Size: 5746
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3871 4736 5034 3871
Size: 865 298 4583
Fragments arranged by size:
4583 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4409 882
Size: 617 2910 2219
Fragments arranged by size:
2910 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3537 493
Size: 711 2333 2702
Fragments arranged by size:
2702 2333 711
BsaBI GATnn'nnATC
Cuts at: 5740 5740
Size: 5746
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3553 3935 4570 275
Size: 53 83 186 936 2020 382 635 1451
Fragments arranged by size:
2020 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4179 4577 4851 4998 5409 4179
Size: 398 274 147 411 4516
Fragments arranged by size:
4516 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5521
Fragments arranged by size:
5521 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2876 1186
Size: 1690 4056
BseYI C'CCAG_C
Cuts at: 2623 4949 5445 2623
Size: 2326 496 2924
Fragments arranged by size:
2924 2326 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2018 1163
Size: 573 282 4891
Fragments arranged by size:
4891 573 282
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3196 3957 4985 1540
Size: 275 186 1195 761 1028 2301
Fragments arranged by size:
2301 1195 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2283 2583 2660 3000 3807 3892 4963 2283
Size: 300 77 340 807 85 1071 3066
Fragments arranged by size:
3066 1071 807 340 300 85 77
BsiWI C'GTAC_G
Cuts at: 2093 2093
Size: 5746
BsmI GAATG_Cn'
Cuts at: 3257 3350 3257
Size: 93 5653
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2050 2283 2327 2509 2583 2660 2671
Size: 399 260 233 44 182 74 77 11
Cuts at: 2671 2792 2947 3000 3065 3807 3892 4963 1391
Size: 121 155 53 65 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 260 233 182 155
121 85 77 74 65 53 44 11
BspEI T'CCGG_A
Cuts at: 5409 5409
Size: 5746
BspHI T'CATG_A
Cuts at: 3635 5365 3635
Size: 1730 4016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3101 844
Size: 2257 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2395 3633 1394
Size: 429 572 1238 3507
Fragments arranged by size:
3507 1238 572 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4241 4415 65
Size: 1183 2993 174 1396
Fragments arranged by size:
2993 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4390 4577 1700
Size: 110 2580 187 2869
Fragments arranged by size:
2869 2580 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5746
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5746
BssSI C'ACGA_G
Cuts at: 3806 4818 3806
Size: 1012 4734
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5746
BstBI TT'CG_AA
Cuts at: 4550 4550
Size: 5746
BstEII G'GTnAC_C
Cuts at: 1184 2434 2467 1184
Size: 1250 33 4463
Fragments arranged by size:
4463 1250 33
BstXI CCAn_nnnn'nTGG
Cuts at: 2305 2305
Size: 5746
BstYI r'GATC_y
Cuts at: 1972 2263 3076 3443 3828 3845 5297 5383 5395
Size: 291 813 367 385 17 1452 86 12
Cuts at: 5395 5741 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 813 385 367 346 291 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2549 513
Size: 552 1484 3710
Fragments arranged by size:
3710 1484 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5007
Fragments arranged by size:
5007 683 56
BtsI GCAGTG_nn'
Cuts at: 2014 2079 2211 3262 4056 4076 2014
Size: 65 132 1051 794 20 3684
Fragments arranged by size:
3684 1051 794 132 65 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5746
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5711
DraI TTT'AAA
Cuts at: 3050 3050
Size: 5746
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4753 809
Size: 507 3437 1802
Fragments arranged by size:
3437 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2076 2546 3193
Size: 54 1425 50 275 18 246 470 647
Cuts at: 3193 4082 5711 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 647 470 275 246 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3193 1537
Size: 275 1381 4090
Fragments arranged by size:
4090 1381 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3676 1353
Size: 2323 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4333 4707 4853 387
Size: 1064 138 2744 374 146 1280
Fragments arranged by size:
2744 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2281 2658 2281
Size: 377 5369
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2361 2513 2531 2556 3809 4406
Size: 393 407 322 152 18 25 1253 597
Cuts at: 4406 5193 5461 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1253 787 597 407 393 322 268
152 25 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2054 2606 2054
Size: 552 5194
EcoO109I rG'GnC_Cy
Cuts at: 2047 2505 2506 2047
Size: 458 1 5287
Fragments arranged by size:
5287 458 1
EcoRI G'AATT_C
Cuts at: 3055 3433 3055
Size: 378 5368
FspI TGC'GCA
Cuts at: 4252 4252
Size: 5746
HaeII r_GCGC'y
Cuts at: 1678 2500 4893 1678
Size: 822 2393 2531
Fragments arranged by size:
2531 2393 822
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2461 2493 2695 2727 4387
Size: 32 135 32 889 32 202 32 1660
Cuts at: 4387 4419 4461 4493 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1660 889 202 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2714 2738 3090 3336 669
Size: 988 281 776 24 352 246 3079
Fragments arranged by size:
3079 988 776 352 281 246 24
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5746
HpaI GTT'AAC
Cuts at: 3336 3336
Size: 5746
KpnI G_GTAC'C
Cuts at: 3071 3071
Size: 5746
MfeI C'AATT_G
Cuts at: 3345 3345
Size: 5746
MluI A'CGCG_T
Cuts at: 5622 5622
Size: 5746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2622 2726 2897 4860 5044 1305
Size: 1317 104 171 1963 184 2007
Fragments arranged by size:
2007 1963 1317 184 171 104
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2078 10
Size: 54 1425 343 246 3678
Fragments arranged by size:
3678 1425 343 246 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2576 3023 3704 4063 4222 518
Size: 648 1410 447 681 359 159 2042
Fragments arranged by size:
2042 1410 681 648 447 359 159
MspA1I CmG'CkG
Cuts at: 1606 2158 2293 2385 2551 2623 2683 2965 3162
Size: 552 135 92 166 72 60 282 197
Cuts at: 3162 3839 4987 5232 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 552 282 245 197 166
135 92 72 60
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5194
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5746
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5746
NotI GC'GGCC_GC
Cuts at: 3193 3193
Size: 5746
NsiI A_TGCA'T
Cuts at: 2887 2887
Size: 5746
NspI r_CATG'y
Cuts at: 1132 1229 2137 2974 3104 4649 1132
Size: 97 908 837 130 1545 2229
Fragments arranged by size:
2229 1545 908 837 130 97
PasI CC'CwG_GG
Cuts at: 1348 2501 1348
Size: 1153 4593
PciI A'CATG_T
Cuts at: 4645 4645
Size: 5746
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5746
PfoI T'CCnGG_A
Cuts at: 2792 2792
Size: 5746
PmeI GTTT'AAAC
Cuts at: 3050 3050
Size: 5746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3815 3847 5328 5360 3815
Size: 32 1481 32 4201
Fragments arranged by size:
4201 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2683 2733 1613
Size: 1070 50 4626
Fragments arranged by size:
4626 1070 50
PsiI TTA'TAA
Cuts at: 3316 3316
Size: 5746
PspOMI G'GGCC_C
Cuts at: 2046 2505 2667 2046
Size: 459 162 5125
Fragments arranged by size:
5125 459 162
PstI C_TGCA'G
Cuts at: 830 2292 3098 830
Size: 1462 806 3478
Fragments arranged by size:
3478 1462 806
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5746
PvuII CAG'CTG
Cuts at: 2293 2385 2623 2683 2293
Size: 92 238 60 5356
Fragments arranged by size:
5356 238 92 60
SacI G_AGCT'C
Cuts at: 2283 2660 2283
Size: 377 5369
SacII CC_GC'GG
Cuts at: 2552 2552
Size: 5746
SalI G'TCGA_C
Cuts at: 1936 2712 3088 1936
Size: 776 376 4594
Fragments arranged by size:
4594 776 376
SbfI CC_TGCA'GG
Cuts at: 3098 3098
Size: 5746
ScaI AGT'ACT
Cuts at: 3994 5706 3994
Size: 1712 4034
SfcI C'TryA_G
Cuts at: 826 1045 2186 2288 3094 4229 4910 5101 826
Size: 219 1141 102 806 1135 681 191 1471
Fragments arranged by size:
1471 1141 1135 806 681 219 191 102
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5746
SmaI CCC'GGG
Cuts at: 1872 3073 1872
Size: 1201 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3850 4751 5013 820
Size: 197 926 1907 901 262 1553
Fragments arranged by size:
1907 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3537 493
Size: 3044 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5746
SphI G_CATG'C
Cuts at: 1132 2974 3104 1132
Size: 1842 130 3774
Fragments arranged by size:
3774 1842 130
SspI AAT'ATT
Cuts at: 5 52 3670 5
Size: 47 3618 2081
Fragments arranged by size:
3618 2081 47
StuI AGG'CCT
Cuts at: 1517 2476 1517
Size: 959 4787
StyI C'CwwG_G
Cuts at: 513 1065 2445 3185 513
Size: 552 1380 740 3074
Fragments arranged by size:
3074 1380 740 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2615 3027 3773 3790 3943 4128 4573
Size: 1255 1035 412 746 17 153 185 445
Cuts at: 4573 325
Size: 1498
Fragments arranged by size:
1498 1255 1035 746 445 412 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2814 3992 5704
Size: 274 80 33 51 1443 837 1178 1712
Cuts at: 5704 96
Size: 138
Fragments arranged by size:
1712 1443 1178 837 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5706 5738 5706
Size: 32 5714
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5746
XbaI T'CTAG_A
Cuts at: 3082 3082
Size: 5746
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5746
XmaI C'CCGG_G
Cuts at: 1870 3071 1870
Size: 1201 4545
XmnI GAAnn'nnTTC
Cuts at: 3875 3875
Size: 5746
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3554 276
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspXI PsrI RsrII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI