Restriction map for
pFN21AE1825
(Circular) (Six-base) MAPSORT of: pFN21AE1825.seq Check: 5988 from: 1 to: 5728
pFN21AE1825 5728 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3538 278
Size: 53 83 186 936 2002 2468
Fragments arranged by size:
2468 2002 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3071 1937
Size: 1134 4594
Acc65I G'GTAC_C
Cuts at: 3049 3049
Size: 5728
AclI AA'CG_TT
Cuts at: 1563 3855 4228 1563
Size: 2292 373 3063
Fragments arranged by size:
3063 2292 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2081 2497 2532 2714 3791 5175 5443 1632
Size: 449 416 35 182 1077 1384 268 1917
Fragments arranged by size:
1917 1384 1077 449 416 268 182 35
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5531
AflIII A'CryG_T
Cuts at: 1203 4627 5604 1203
Size: 3424 977 1327
Fragments arranged by size:
3424 1327 977
AgeI A'CCGG_T
Cuts at: 4559 4559
Size: 5728
AhdI GACnn_n'nnGTC
Cuts at: 4457 4457
Size: 5728
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5728
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5728
AlwNI CAG_nnn'CTG
Cuts at: 2692 3471 5043 2692
Size: 779 1572 3377
Fragments arranged by size:
3377 1572 779
ApaLI G'TGCA_C
Cuts at: 3785 4941 3785
Size: 1156 4572
ApoI r'AATT_y
Cuts at: 1493 2160 2304 2606 3037 3263 3415 5524 1493
Size: 667 144 302 431 226 152 2109 1697
Fragments arranged by size:
2109 1697 667 431 302 226 152 144
AseI AT'TA_AT
Cuts at: 160 1032 2777 4282 160
Size: 872 1745 1505 1606
Fragments arranged by size:
1745 1606 1505 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5728
AvaI C'yCGr_G
Cuts at: 1870 1943 3053 1870
Size: 73 1110 4545
Fragments arranged by size:
4545 1110 73
BamHI G'GATC_C
Cuts at: 3058 3425 3058
Size: 367 5361
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3049 4504 618
Size: 325 217 627 1262 1455 1842
Fragments arranged by size:
1842 1455 1262 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2241 3047 1391
Size: 850 806 4072
Fragments arranged by size:
4072 850 806
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2332 2978 928
Size: 971 433 646 3678
Fragments arranged by size:
3678 971 646 433
BbvCI CC'TCA_GC
Cuts at: 2921 2921
Size: 5728
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3919 3953 1903
Size: 34 50 34 1898 34 3678
Fragments arranged by size:
3678 1898 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3620 4830 3620
Size: 1210 4518
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5728
BfrBI ATG'CAT
Cuts at: 2454 2555 2454
Size: 101 5627
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4339 136
Size: 107 122 71 631 605 2667 1525
Fragments arranged by size:
2667 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5723 5723
Size: 5728
BlpI GC'TnA_GC
Cuts at: 3015 3145 3015
Size: 130 5598
Bme1580I G_kGCm'C
Cuts at: 1790 3789 4945 1790
Size: 1999 1156 2573
Fragments arranged by size:
2573 1999 1156
BmgBI CAC'GTC
Cuts at: 1318 2296 1318
Size: 978 4750
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3459 4417 442
Size: 416 53 83 399 24 2042 958 1753
Fragments arranged by size:
2042 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5728
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3030 3030
Size: 5728
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2022 2397 4388 1239
Size: 783 375 1991 2579
Fragments arranged by size:
2579 1991 783 375
Bpu10I CC'TnA_GC
Cuts at: 2243 2921 2243
Size: 678 5050
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2202 2876 3853 4718 5016 2202
Size: 674 977 865 298 2914
Fragments arranged by size:
2914 977 865 674 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3001 4391 882
Size: 617 1502 1390 2219
Fragments arranged by size:
2219 1502 1390 617
BsaAI yAC'GTr
Cuts at: 493 1204 3519 493
Size: 711 2315 2702
Fragments arranged by size:
2702 2315 711
BsaBI GATnn'nnATC
Cuts at: 5722 5722
Size: 5728
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3535 3917 4552 275
Size: 53 83 186 936 2002 382 635 1451
Fragments arranged by size:
2002 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4161 4559 4833 4980 5391 4161
Size: 398 274 147 411 4498
Fragments arranged by size:
4498 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2329 2359 1178
Size: 30 165 30 926 30 4547
Fragments arranged by size:
4547 926 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2879 1186
Size: 1693 4035
BseYI C'CCAG_C
Cuts at: 4931 5427 4931
Size: 496 5232
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2350 2622 1163
Size: 573 614 272 4269
Fragments arranged by size:
4269 614 573 272
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3178 3939 4967 1540
Size: 275 186 1177 761 1028 2301
Fragments arranged by size:
2301 1177 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3789 3874 4945 3789
Size: 85 1071 4572
Fragments arranged by size:
4572 1071 85
BsmI GAATG_Cn'
Cuts at: 2456 3239 3332 2456
Size: 783 93 4852
Fragments arranged by size:
4852 783 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2241 3047 3789 3874 4945 1391
Size: 399 451 806 742 85 1071 2174
Fragments arranged by size:
2174 1071 806 742 451 399 85
BspEI T'CCGG_A
Cuts at: 5391 5391
Size: 5728
BspHI T'CATG_A
Cuts at: 3617 5347 3617
Size: 1730 3998
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2796 3083 844
Size: 1952 287 3489
Fragments arranged by size:
3489 1952 287
BsrBI CCG'CTC
Cuts at: 1394 1823 3615 1394
Size: 429 1792 3507
Fragments arranged by size:
3507 1792 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4223 4397 65
Size: 1183 2975 174 1396
Fragments arranged by size:
2975 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4372 4559 1700
Size: 110 2562 187 2869
Fragments arranged by size:
2869 2562 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5728
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5728
BssSI C'ACGA_G
Cuts at: 3788 4800 3788
Size: 1012 4716
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5728
BstBI TT'CG_AA
Cuts at: 4532 4532
Size: 5728
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5728
BstXI CCAn_nnnn'nTGG
Cuts at: 2143 2143
Size: 5728
BstYI r'GATC_y
Cuts at: 1972 3058 3425 3810 3827 5279 5365 5377 5723
Size: 1086 367 385 17 1452 86 12 346
Cuts at: 5723 1972
Size: 1977
Fragments arranged by size:
1977 1452 1086 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4989
Fragments arranged by size:
4989 683 56
BtsI GCAGTG_nn'
Cuts at: 3244 4038 4058 3244
Size: 794 20 4914
Fragments arranged by size:
4914 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5728
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5693
DraI TTT'AAA
Cuts at: 3032 3032
Size: 5728
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4735 809
Size: 507 3419 1802
Fragments arranged by size:
3419 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3175 4064 5693
Size: 54 1425 50 275 18 1345 889 1629
Cuts at: 5693 8
Size: 43
Fragments arranged by size:
1629 1425 1345 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3175 1537
Size: 275 1363 4090
Fragments arranged by size:
4090 1363 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2999 3658 1353
Size: 1646 659 3423
Fragments arranged by size:
3423 1646 659
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4315 4689 4835 387
Size: 1064 138 2726 374 146 1280
Fragments arranged by size:
2726 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2022 2081 2397 2497 2532 2714 3791
Size: 393 390 59 316 100 35 182 1077
Cuts at: 3791 4388 5175 5443 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1077 787 597 393 390 316 268
182 100 59 35
EcoRI G'AATT_C
Cuts at: 3037 3415 3037
Size: 378 5350
FspI TGC'GCA
Cuts at: 4234 4234
Size: 5728
HaeII r_GCGC'y
Cuts at: 1678 4875 1678
Size: 3197 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2439 2471 4369 4401 4443
Size: 32 135 32 867 32 1898 32 42
Cuts at: 4443 4475 1373
Size: 32 2626
Fragments arranged by size:
2626 1898 867 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2532 3072 3318 669
Size: 988 281 594 540 246 3079
Fragments arranged by size:
3079 988 594 540 281 246
HindIII A'AGCT_T
Cuts at: 748 2696 748
Size: 1948 3780
HpaI GTT'AAC
Cuts at: 3318 3318
Size: 5728
KpnI G_GTAC'C
Cuts at: 3053 3053
Size: 5728
MfeI C'AATT_G
Cuts at: 3327 3327
Size: 5728
MluI A'CGCG_T
Cuts at: 5604 5604
Size: 5728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2472 4842 5026 1305
Size: 1167 2370 184 2007
Fragments arranged by size:
2370 2007 1167 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3906
Fragments arranged by size:
3906 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2738 3686 4045 4204 518
Size: 648 1572 948 359 159 2042
Fragments arranged by size:
2042 1572 948 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3144 3821 4969 5214 1606
Size: 1538 677 1148 245 2120
Fragments arranged by size:
2120 1538 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5176
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5728
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5728
NotI GC'GGCC_GC
Cuts at: 3175 3175
Size: 5728
NsiI A_TGCA'T
Cuts at: 2456 2557 2456
Size: 101 5627
NspI r_CATG'y
Cuts at: 1132 1229 3086 4631 1132
Size: 97 1857 1545 2229
Fragments arranged by size:
2229 1857 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5728
PciI A'CATG_T
Cuts at: 4627 4627
Size: 5728
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5728
PmeI GTTT'AAAC
Cuts at: 3032 3032
Size: 5728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3797 3829 5310 5342 3797
Size: 32 1481 32 4183
Fragments arranged by size:
4183 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2802 1613
Size: 1189 4539
PsiI TTA'TAA
Cuts at: 3298 3298
Size: 5728
PstI C_TGCA'G
Cuts at: 830 3080 830
Size: 2250 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5728
SalI G'TCGA_C
Cuts at: 1936 3070 1936
Size: 1134 4594
SbfI CC_TGCA'GG
Cuts at: 3080 3080
Size: 5728
ScaI AGT'ACT
Cuts at: 3976 5688 3976
Size: 1712 4016
SfcI C'TryA_G
Cuts at: 826 1045 2121 3076 4211 4892 5083 826
Size: 219 1076 955 1135 681 191 1471
Fragments arranged by size:
1471 1135 1076 955 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5728
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5728
SmaI CCC'GGG
Cuts at: 1872 3055 1872
Size: 1183 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2217 2891 3832 4733 4995 820
Size: 197 926 274 674 941 901 262 1553
Fragments arranged by size:
1553 941 926 901 674 274 262 197
SnaBI TAC'GTA
Cuts at: 493 3519 493
Size: 3026 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5728
SphI G_CATG'C
Cuts at: 1132 3086 1132
Size: 1954 3774
SspI AAT'ATT
Cuts at: 5 52 2317 2380 3652 5
Size: 47 2265 63 1272 2081
Fragments arranged by size:
2265 2081 1272 63 47
StuI AGG'CCT
Cuts at: 1517 2825 1517
Size: 1308 4420
StyI C'CwwG_G
Cuts at: 513 1065 3167 513
Size: 552 2102 3074
Fragments arranged by size:
3074 2102 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2384 3755 3772 3925 4110 4555 325
Size: 1255 804 1371 17 153 185 445 1498
Fragments arranged by size:
1498 1371 1255 804 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3974 5686 96
Size: 274 80 33 51 1443 1997 1712 138
Fragments arranged by size:
1997 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5688 5720 5688
Size: 32 5696
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5728
XbaI T'CTAG_A
Cuts at: 3064 3064
Size: 5728
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5728
XmaI C'CCGG_G
Cuts at: 1870 3053 1870
Size: 1183 4545
XmnI GAAnn'nnTTC
Cuts at: 3857 3857
Size: 5728
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3536 276
Size: 53 83 186 936 2002 2468
Fragments arranged by size:
2468 2002 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI