Restriction map for
pFN21AE1821
(Circular) (Six-base) MAPSORT of: pFN21AE1821.seq Check: 699 from: 1 to: 5710
pFN21AE1821 5710 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2848 2848
Size: 5710
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2570 3520 278
Size: 53 83 186 936 1034 950 2468
Fragments arranged by size:
2468 1034 950 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3053 1937
Size: 1116 4594
Acc65I G'GTAC_C
Cuts at: 3031 3031
Size: 5710
AclI AA'CG_TT
Cuts at: 1563 3837 4210 1563
Size: 2274 373 3063
Fragments arranged by size:
3063 2274 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2678 3773 5157 5425 1632
Size: 1046 1095 1384 268 1917
Fragments arranged by size:
1917 1384 1095 1046 268
AfeI AGC'GCT
Cuts at: 2978 2978
Size: 5710
AflII C'TTAA_G
Cuts at: 820 1017 2047 820
Size: 197 1030 4483
Fragments arranged by size:
4483 1030 197
AflIII A'CryG_T
Cuts at: 1203 2207 2832 4609 5586 1203
Size: 1004 625 1777 977 1327
Fragments arranged by size:
1777 1327 1004 977 625
AgeI A'CCGG_T
Cuts at: 4541 4541
Size: 5710
AhdI GACnn_n'nnGTC
Cuts at: 4439 4439
Size: 5710
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5710
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2790 1184
Size: 1606 4104
AlwNI CAG_nnn'CTG
Cuts at: 3453 5025 3453
Size: 1572 4138
ApaLI G'TGCA_C
Cuts at: 2861 3767 4923 2861
Size: 906 1156 3648
Fragments arranged by size:
3648 1156 906
ApoI r'AATT_y
Cuts at: 1493 2327 3019 3245 3397 5506 1493
Size: 834 692 226 152 2109 1697
Fragments arranged by size:
2109 1697 834 692 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4264 160
Size: 872 3232 1606
Fragments arranged by size:
3232 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
AvaI C'yCGr_G
Cuts at: 1870 1943 2445 3035 1870
Size: 73 502 590 4545
Fragments arranged by size:
4545 590 502 73
BamHI G'GATC_C
Cuts at: 2389 3040 3407 2389
Size: 651 367 4692
Fragments arranged by size:
4692 651 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3031 4486 618
Size: 325 217 627 1244 1455 1842
Fragments arranged by size:
1842 1455 1244 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2371 3029 1391
Size: 980 658 4072
Fragments arranged by size:
4072 980 658
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4739
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3901 3935 1903
Size: 34 1964 34 3678
Fragments arranged by size:
3678 1964 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3602 4812 3602
Size: 1210 4500
BclI T'GATC_A
Cuts at: 1548 2332 1548
Size: 784 4926
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4321 136
Size: 107 122 71 631 605 2649 1525
Fragments arranged by size:
2649 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5705 5705
Size: 5710
BlpI GC'TnA_GC
Cuts at: 2763 3127 2763
Size: 364 5346
Bme1580I G_kGCm'C
Cuts at: 1790 2865 3771 4927 1790
Size: 1075 906 1156 2573
Fragments arranged by size:
2573 1156 1075 906
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5710
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2092 3441 4399
Size: 416 53 83 399 24 675 1349 958
Cuts at: 4399 442
Size: 1753
Fragments arranged by size:
1753 1349 958 675 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2048 2685 4370 1239
Size: 809 637 1685 2579
Fragments arranged by size:
2579 1685 809 637
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3835 4700 4998 3835
Size: 865 298 4547
Fragments arranged by size:
4547 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4373 882
Size: 617 2874 2219
Fragments arranged by size:
2874 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3501 493
Size: 711 2297 2702
Fragments arranged by size:
2702 2297 711
BsaBI GATnn'nnATC
Cuts at: 5704 5704
Size: 5710
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2567 3517 3899 4534
Size: 53 83 186 936 1034 950 382 635
Cuts at: 4534 275
Size: 1451
Fragments arranged by size:
1451 1034 950 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2795 2824 4143 4541 4815 4962 5373 2795
Size: 29 1319 398 274 147 411 3132
Fragments arranged by size:
3132 1319 411 398 274 147 29
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5485
Fragments arranged by size:
5485 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2141 2525 1186
Size: 955 384 4371
Fragments arranged by size:
4371 955 384
BseYI C'CCAG_C
Cuts at: 2370 4913 5409 2370
Size: 2543 496 2671
Fragments arranged by size:
2671 2543 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2760 1163
Size: 573 1024 4113
Fragments arranged by size:
4113 1024 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3160 3921 4949 1540
Size: 275 186 1159 761 1028 2301
Fragments arranged by size:
2301 1159 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2865 3771 3856 4927 2865
Size: 906 85 1071 3648
Fragments arranged by size:
3648 1071 906 85
BsmI GAATG_Cn'
Cuts at: 3221 3314 3221
Size: 93 5617
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2371 2865 3029 3771 3856 4927 1391
Size: 399 581 494 164 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 581 494 399 164 85
BspEI T'CCGG_A
Cuts at: 2795 5373 2795
Size: 2578 3132
BspHI T'CATG_A
Cuts at: 2941 3599 5329 2941
Size: 658 1730 3322
Fragments arranged by size:
3322 1730 658
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2818 2848 3065 844
Size: 1974 30 217 3489
Fragments arranged by size:
3489 1974 217 30
BsrBI CCG'CTC
Cuts at: 1394 1823 3597 1394
Size: 429 1774 3507
Fragments arranged by size:
3507 1774 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4205 4379 65
Size: 1183 2957 174 1396
Fragments arranged by size:
2957 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4354 4541 1700
Size: 110 2544 187 2869
Fragments arranged by size:
2869 2544 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5710
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5710
BssSI C'ACGA_G
Cuts at: 3770 4782 3770
Size: 1012 4698
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5710
BstBI TT'CG_AA
Cuts at: 4514 4514
Size: 5710
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5710
BstYI r'GATC_y
Cuts at: 1972 2246 2389 3040 3407 3792 3809 5261 5347
Size: 274 143 651 367 385 17 1452 86
Cuts at: 5347 5359 5705 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 651 385 367 346 274 143
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4219
Fragments arranged by size:
4219 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4971
Fragments arranged by size:
4971 683 56
BtsI GCAGTG_nn'
Cuts at: 3226 4020 4040 3226
Size: 794 20 4896
Fragments arranged by size:
4896 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5710
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5675
DraI TTT'AAA
Cuts at: 2617 3014 2617
Size: 397 5313
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4717 809
Size: 507 3401 1802
Fragments arranged by size:
3401 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2196 2258
Size: 54 1425 50 275 18 204 162 62
Cuts at: 2258 3157 4046 5675 8
Size: 899 889 1629 43
Fragments arranged by size:
1629 1425 899 889 275 204 162 62
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3157 1537
Size: 275 1345 4090
Fragments arranged by size:
4090 1345 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2940 3640 1353
Size: 1587 700 3423
Fragments arranged by size:
3423 1587 700
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4297 4671 4817 387
Size: 1064 138 2708 374 146 1280
Fragments arranged by size:
2708 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2678 2685 3773 4370 5157 5425
Size: 393 416 630 7 1088 597 787 268
Cuts at: 5425 1239
Size: 1524
Fragments arranged by size:
1524 1088 787 630 597 416 393 268
7
EcoNI CCTnn'n_nnAGG
Cuts at: 2259 2361 2259
Size: 102 5608
EcoO109I rG'GnC_Cy
Cuts at: 2056 2056
Size: 5710
EcoRI G'AATT_C
Cuts at: 3019 3397 3019
Size: 378 5332
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2062 2607 2062
Size: 545 5165
FspI TGC'GCA
Cuts at: 4216 4216
Size: 5710
HaeII r_GCGC'y
Cuts at: 1678 2980 4857 1678
Size: 1302 1877 2531
Fragments arranged by size:
2531 1877 1302
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4351 4383 4425 4457 1373
Size: 32 135 32 2779 32 42 32 2626
Fragments arranged by size:
2779 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3054 3300 669
Size: 988 281 1116 246 3079
Fragments arranged by size:
3079 1116 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2306 2589 2850 748
Size: 1558 283 261 3608
Fragments arranged by size:
3608 1558 283 261
HpaI GTT'AAC
Cuts at: 3300 3300
Size: 5710
KpnI G_GTAC'C
Cuts at: 3035 3035
Size: 5710
MfeI C'AATT_G
Cuts at: 3309 3309
Size: 5710
MluI A'CGCG_T
Cuts at: 2207 5586 2207
Size: 3379 2331
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4824 5008 1305
Size: 3519 184 2007
Fragments arranged by size:
3519 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2198 2260 10
Size: 54 1425 343 366 62 3460
Fragments arranged by size:
3460 1425 366 343 62 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2432 3668 4027 4186 518
Size: 648 1266 1236 359 159 2042
Fragments arranged by size:
2042 1266 1236 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2525 3126 3803 4951 5196 1606
Size: 919 601 677 1148 245 2120
Fragments arranged by size:
2120 1148 919 677 601 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4219
Fragments arranged by size:
4219 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5710
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5710
NotI GC'GGCC_GC
Cuts at: 3157 3157
Size: 5710
NspI r_CATG'y
Cuts at: 1132 1229 2793 2836 3068 4613 1132
Size: 97 1564 43 232 1545 2229
Fragments arranged by size:
2229 1564 1545 232 97 43
PasI CC'CwG_GG
Cuts at: 1348 2059 1348
Size: 711 4999
PciI A'CATG_T
Cuts at: 2832 4609 2832
Size: 1777 3933
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2980 1108
Size: 1872 3838
PmeI GTTT'AAAC
Cuts at: 3014 3014
Size: 5710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3779 3811 5292 5324 3779
Size: 32 1481 32 4165
Fragments arranged by size:
4165 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5710
PsiI TTA'TAA
Cuts at: 3280 3280
Size: 5710
PspXI vC'TCGA_Gb
Cuts at: 2445 2445
Size: 5710
PstI C_TGCA'G
Cuts at: 830 2955 3062 830
Size: 2125 107 3478
Fragments arranged by size:
3478 2125 107
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5710
PvuII CAG'CTG
Cuts at: 2525 2525
Size: 5710
SalI G'TCGA_C
Cuts at: 1936 3052 1936
Size: 1116 4594
SapI GCTCTTCn'nnn_
Cuts at: 2940 2940
Size: 5710
SbfI CC_TGCA'GG
Cuts at: 3062 3062
Size: 5710
ScaI AGT'ACT
Cuts at: 3958 5670 3958
Size: 1712 3998
SfcI C'TryA_G
Cuts at: 826 1045 2951 3058 4193 4874 5065 826
Size: 219 1906 107 1135 681 191 1471
Fragments arranged by size:
1906 1471 1135 681 219 191 107
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5710
SmaI CCC'GGG
Cuts at: 1872 3037 1872
Size: 1165 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2047 2445 3814 4715 4977 820
Size: 197 926 104 398 1369 901 262 1553
Fragments arranged by size:
1553 1369 926 901 398 262 197 104
SnaBI TAC'GTA
Cuts at: 493 3501 493
Size: 3008 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5710
SphI G_CATG'C
Cuts at: 1132 2793 3068 1132
Size: 1661 275 3774
Fragments arranged by size:
3774 1661 275
SspI AAT'ATT
Cuts at: 5 52 3634 5
Size: 47 3582 2081
Fragments arranged by size:
3582 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5710
StyI C'CwwG_G
Cuts at: 513 1065 2004 2255 2292 2988 3149 513
Size: 552 939 251 37 696 161 3074
Fragments arranged by size:
3074 939 696 552 251 161 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2760 3737 3754 3907 4092 4537 325
Size: 1255 1180 977 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1180 977 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3956 5668 96
Size: 274 80 33 51 1443 1979 1712 138
Fragments arranged by size:
1979 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5670 5702 5670
Size: 32 5678
Tth111I GACn'n_nGTC
Cuts at: 1316 2501 1316
Size: 1185 4525
XbaI T'CTAG_A
Cuts at: 3046 3046
Size: 5710
XhoI C'TCGA_G
Cuts at: 1943 2445 1943
Size: 502 5208
XmaI C'CCGG_G
Cuts at: 1870 3035 1870
Size: 1165 4545
XmnI GAAnn'nnTTC
Cuts at: 3839 3839
Size: 5710
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2568 3518 276
Size: 53 83 186 936 1034 950 2468
Fragments arranged by size:
2468 1034 950 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI