Restriction map for
pFN21AE1811
(Circular) (Six-base) MAPSORT of: pFN21AE1811.seq Check: 7091 from: 1 to: 5686
pFN21AE1811 5686 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2838 3496 278
Size: 53 83 186 936 1302 658 2468
Fragments arranged by size:
2468 1302 936 658 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2982 3029 1937
Size: 1045 47 4594
Fragments arranged by size:
4594 1045 47
Acc65I G'GTAC_C
Cuts at: 3007 3007
Size: 5686
AclI AA'CG_TT
Cuts at: 1563 3813 4186 1563
Size: 2250 373 3063
Fragments arranged by size:
3063 2250 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2705 3749 5133 5401 1632
Size: 1073 1044 1384 268 1917
Fragments arranged by size:
1917 1384 1073 1044 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5489
AflIII A'CryG_T
Cuts at: 1203 4585 5562 1203
Size: 3382 977 1327
Fragments arranged by size:
3382 1327 977
AgeI A'CCGG_T
Cuts at: 4517 4517
Size: 5686
AhdI GACnn_n'nnGTC
Cuts at: 2105 4415 2105
Size: 2310 3376
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5686
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5686
AlwNI CAG_nnn'CTG
Cuts at: 3429 5001 3429
Size: 1572 4114
ApaLI G'TGCA_C
Cuts at: 3743 4899 3743
Size: 1156 4530
ApoI r'AATT_y
Cuts at: 1493 2072 2230 2995 3221 3373 5482 1493
Size: 579 158 765 226 152 2109 1697
Fragments arranged by size:
2109 1697 765 579 226 158 152
AseI AT'TA_AT
Cuts at: 160 1032 4240 160
Size: 872 3208 1606
Fragments arranged by size:
3208 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
AvaI C'yCGr_G
Cuts at: 1870 1943 3011 1870
Size: 73 1068 4545
Fragments arranged by size:
4545 1068 73
BamHI G'GATC_C
Cuts at: 2928 3016 3383 2928
Size: 88 367 5231
Fragments arranged by size:
5231 367 88
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2894 3007 4462 618
Size: 325 217 627 1107 113 1455 1842
Fragments arranged by size:
1842 1455 1107 627 325 217 113
BanII G_rGCy'C
Cuts at: 1391 3005 1391
Size: 1614 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2101 2268 928
Size: 971 202 167 4346
Fragments arranged by size:
4346 971 202 167
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3877 3911 1903
Size: 34 1940 34 3678
Fragments arranged by size:
3678 1940 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3578 4788 3578
Size: 1210 4476
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5686
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4297 136
Size: 107 122 71 631 605 2625 1525
Fragments arranged by size:
2625 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5681 5681
Size: 5686
BlpI GC'TnA_GC
Cuts at: 3103 3103
Size: 5686
Bme1580I G_kGCm'C
Cuts at: 1790 3747 4903 1790
Size: 1957 1156 2573
Fragments arranged by size:
2573 1957 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5686
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3417 4375 442
Size: 416 53 83 399 24 2000 958 1753
Fragments arranged by size:
2000 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5686
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2578 2935 2578
Size: 357 5329
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4346 1239
Size: 3107 2579
Bpu10I CC'TnA_GC
Cuts at: 2346 2805 2346
Size: 459 5227
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3811 4676 4974 3811
Size: 865 298 4523
Fragments arranged by size:
4523 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2344 2564 4349 882
Size: 617 845 220 1785 2219
Fragments arranged by size:
2219 1785 845 617 220
BsaAI yAC'GTr
Cuts at: 493 1204 2584 3477 493
Size: 711 1380 893 2702
Fragments arranged by size:
2702 1380 893 711
BsaBI GATnn'nnATC
Cuts at: 5680 5680
Size: 5686
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2835 3493 3875 4510
Size: 53 83 186 936 1302 658 382 635
Cuts at: 4510 275
Size: 1451
Fragments arranged by size:
1451 1302 936 658 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4119 4517 4791 4938 5349 4119
Size: 398 274 147 411 4456
Fragments arranged by size:
4456 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5461
Fragments arranged by size:
5461 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5686
BseYI C'CCAG_C
Cuts at: 4889 5385 4889
Size: 496 5190
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5113
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3136 3897 4925 1540
Size: 275 186 1135 761 1028 2301
Fragments arranged by size:
2301 1135 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2120 2327 3747 3832 4903 2120
Size: 207 1420 85 1071 2903
Fragments arranged by size:
2903 1420 1071 207 85
BsmI GAATG_Cn'
Cuts at: 2288 3197 3290 2288
Size: 909 93 4684
Fragments arranged by size:
4684 909 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2120 2327 3005 3747 3832 4903 1391
Size: 399 330 207 678 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 678 399 330 207 85
BspEI T'CCGG_A
Cuts at: 5349 5349
Size: 5686
BspHI T'CATG_A
Cuts at: 3575 5305 3575
Size: 1730 3956
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3041 844
Size: 2197 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3573 1394
Size: 429 1750 3507
Fragments arranged by size:
3507 1750 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2460 4181 4355 65
Size: 1183 1212 1721 174 1396
Fragments arranged by size:
1721 1396 1212 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4330 4517 1700
Size: 110 2520 187 2869
Fragments arranged by size:
2869 2520 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5686
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5686
BssSI C'ACGA_G
Cuts at: 3746 4758 3746
Size: 1012 4674
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5686
BstBI TT'CG_AA
Cuts at: 4490 4490
Size: 5686
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5686
BstYI r'GATC_y
Cuts at: 1972 2928 3016 3383 3768 3785 5237 5323 5335
Size: 956 88 367 385 17 1452 86 12
Cuts at: 5335 5681 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 956 385 367 346 88 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2924 513
Size: 552 1859 3275
Fragments arranged by size:
3275 1859 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4947
Fragments arranged by size:
4947 683 56
BtsI GCAGTG_nn'
Cuts at: 2396 2426 3202 3996 4016 2396
Size: 30 776 794 20 4066
Fragments arranged by size:
4066 794 776 30 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5686
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5651
DraI TTT'AAA
Cuts at: 2990 2990
Size: 5686
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2212 2450 4693 809
Size: 507 896 238 2243 1802
Fragments arranged by size:
2243 1802 896 507 238
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2495 2841 3133
Size: 54 1425 50 275 18 665 346 292
Cuts at: 3133 4022 5651 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 665 346 292 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3133 1537
Size: 275 1321 4090
Fragments arranged by size:
4090 1321 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2135 3616 1353
Size: 782 1481 3423
Fragments arranged by size:
3423 1481 782
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4273 4647 4793 387
Size: 1064 138 2684 374 146 1280
Fragments arranged by size:
2684 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2705 3749 4346 5133 5401 1239
Size: 393 1073 1044 597 787 268 1524
Fragments arranged by size:
1524 1073 1044 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2173 2761 2881 2173
Size: 588 120 4978
Fragments arranged by size:
4978 588 120
EcoRI G'AATT_C
Cuts at: 2072 2995 3373 2072
Size: 923 378 4385
Fragments arranged by size:
4385 923 378
EcoRV GAT'ATC
Cuts at: 2967 2967
Size: 5686
FspI TGC'GCA
Cuts at: 4192 4192
Size: 5686
HaeII r_GCGC'y
Cuts at: 1678 4833 1678
Size: 3155 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2091 2123 2711 2743 4327
Size: 32 135 32 519 32 588 32 1584
Cuts at: 4327 4359 4401 4433 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1584 588 519 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3030 3276 669
Size: 988 281 1092 246 3079
Fragments arranged by size:
3079 1092 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5686
HpaI GTT'AAC
Cuts at: 3276 3276
Size: 5686
KpnI G_GTAC'C
Cuts at: 3011 3011
Size: 5686
MfeI C'AATT_G
Cuts at: 3285 3285
Size: 5686
MluI A'CGCG_T
Cuts at: 5562 5562
Size: 5686
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2343 2652 4800 4984 1305
Size: 1038 309 2148 184 2007
Fragments arranged by size:
2148 2007 1038 309 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2497 10
Size: 54 1425 343 665 3199
Fragments arranged by size:
3199 1425 665 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3644 4003 4162 518
Size: 648 2478 359 159 2042
Fragments arranged by size:
2478 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3102 3779 4927 5172 1606
Size: 1496 677 1148 245 2120
Fragments arranged by size:
2120 1496 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2924 513
Size: 552 1859 3275
Fragments arranged by size:
3275 1859 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5686
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5686
NotI GC'GGCC_GC
Cuts at: 3133 3133
Size: 5686
NspI r_CATG'y
Cuts at: 1132 1229 3044 4589 1132
Size: 97 1815 1545 2229
Fragments arranged by size:
2229 1815 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2781 1348
Size: 1433 4253
PciI A'CATG_T
Cuts at: 4585 4585
Size: 5686
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5686
PfoI T'CCnGG_A
Cuts at: 2715 2715
Size: 5686
PmeI GTTT'AAAC
Cuts at: 2990 2990
Size: 5686
PmlI CAC'GTG
Cuts at: 2584 2584
Size: 5686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3755 3787 5268 5300 3755
Size: 32 1481 32 4141
Fragments arranged by size:
4141 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2761 2881 2761
Size: 120 5566
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5686
PsiI TTA'TAA
Cuts at: 3256 3256
Size: 5686
PstI C_TGCA'G
Cuts at: 830 3038 830
Size: 2208 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5686
SalI G'TCGA_C
Cuts at: 1936 3028 1936
Size: 1092 4594
SbfI CC_TGCA'GG
Cuts at: 3038 3038
Size: 5686
ScaI AGT'ACT
Cuts at: 3934 5646 3934
Size: 1712 3974
SexAI A'CCwGG_T
Cuts at: 2757 2757
Size: 5686
SfcI C'TryA_G
Cuts at: 826 1045 2589 3034 4169 4850 5041 826
Size: 219 1544 445 1135 681 191 1471
Fragments arranged by size:
1544 1471 1135 681 445 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5686
SmaI CCC'GGG
Cuts at: 1872 3013 1872
Size: 1141 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3790 4691 4953 820
Size: 197 926 1847 901 262 1553
Fragments arranged by size:
1847 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3477 493
Size: 2984 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5686
SphI G_CATG'C
Cuts at: 1132 3044 1132
Size: 1912 3774
SspI AAT'ATT
Cuts at: 5 52 2657 3610 5
Size: 47 2605 953 2081
Fragments arranged by size:
2605 2081 953 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5686
StyI C'CwwG_G
Cuts at: 513 1065 2260 2334 2575 2924 3125 513
Size: 552 1195 74 241 349 201 3074
Fragments arranged by size:
3074 1195 552 349 241 201 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2010 2759 3713 3730 3883 4068 4513
Size: 1255 430 749 954 17 153 185 445
Cuts at: 4513 325
Size: 1498
Fragments arranged by size:
1498 1255 954 749 445 430 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3932 5644 96
Size: 274 80 33 51 1443 1955 1712 138
Fragments arranged by size:
1955 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5646 5678 5646
Size: 32 5654
Tth111I GACn'n_nGTC
Cuts at: 1316 2759 1316
Size: 1443 4243
XbaI T'CTAG_A
Cuts at: 3022 3022
Size: 5686
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2717 2939 2717
Size: 222 5464
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5686
XmaI C'CCGG_G
Cuts at: 1870 3011 1870
Size: 1141 4545
XmnI GAAnn'nnTTC
Cuts at: 2288 3815 2288
Size: 1527 4159
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2836 3494 276
Size: 53 83 186 936 1302 658 2468
Fragments arranged by size:
2468 1302 936 658 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI