Restriction map for
pFN21AE1808
(Circular) (Six-base) MAPSORT of: pFN21AE1808.seq Check: 1683 from: 1 to: 5680
pFN21AE1808 5680 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3490 278
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2208 2771 3023 1937
Size: 271 563 252 4594
Fragments arranged by size:
4594 563 271 252
Acc65I G'GTAC_C
Cuts at: 2421 3001 2421
Size: 580 5100
AclI AA'CG_TT
Cuts at: 1563 3807 4180 1563
Size: 2244 373 3063
Fragments arranged by size:
3063 2244 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3743 5127 5395 1632
Size: 2111 1384 268 1917
Fragments arranged by size:
2111 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5483
AflIII A'CryG_T
Cuts at: 1203 2655 4579 5556 1203
Size: 1452 1924 977 1327
Fragments arranged by size:
1924 1452 1327 977
AgeI A'CCGG_T
Cuts at: 4511 4511
Size: 5680
AhdI GACnn_n'nnGTC
Cuts at: 4409 4409
Size: 5680
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5680
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5680
AlwNI CAG_nnn'CTG
Cuts at: 3423 4995 3423
Size: 1572 4108
ApaLI G'TGCA_C
Cuts at: 2108 3737 4893 2108
Size: 1629 1156 2895
Fragments arranged by size:
2895 1629 1156
ApoI r'AATT_y
Cuts at: 1493 2989 3215 3367 5476 1493
Size: 1496 226 152 2109 1697
Fragments arranged by size:
2109 1697 1496 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4234 160
Size: 872 3202 1606
Fragments arranged by size:
3202 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
AvaI C'yCGr_G
Cuts at: 1870 1943 3005 1870
Size: 73 1062 4545
Fragments arranged by size:
4545 1062 73
BamHI G'GATC_C
Cuts at: 3010 3377 3010
Size: 367 5313
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2394 2421 2934 3001 4456
Size: 325 217 627 607 27 513 67 1455
Cuts at: 4456 618
Size: 1842
Fragments arranged by size:
1842 1455 627 607 513 325 217 67
27
BanII G_rGCy'C
Cuts at: 1391 2479 2999 1391
Size: 1088 520 4072
Fragments arranged by size:
4072 1088 520
BbeI G_GCGC'C
Cuts at: 2938 2938
Size: 5680
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2590 928
Size: 971 691 4018
Fragments arranged by size:
4018 971 691
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3871 3905 1903
Size: 34 1934 34 3678
Fragments arranged by size:
3678 1934 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3572 4782 3572
Size: 1210 4470
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5680
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4291 136
Size: 107 122 71 631 605 2619 1525
Fragments arranged by size:
2619 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5675 5675
Size: 5680
BlpI GC'TnA_GC
Cuts at: 3097 3097
Size: 5680
Bme1580I G_kGCm'C
Cuts at: 1790 2112 2467 3741 4897 1790
Size: 322 355 1274 1156 2573
Fragments arranged by size:
2573 1274 1156 355 322
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5680
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2122 2129 3411
Size: 416 53 83 399 24 705 7 1282
Cuts at: 3411 4369 442
Size: 958 1753
Fragments arranged by size:
1753 1282 958 705 416 399 83 53
24 7
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5680
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2557 2559 2557
Size: 2 5678
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4340 1239
Size: 3101 2579
Bpu10I CC'TnA_GC
Cuts at: 2585 2585
Size: 5680
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3805 4670 4968 3805
Size: 865 298 4517
Fragments arranged by size:
4517 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4343 882
Size: 617 2844 2219
Fragments arranged by size:
2844 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2658 3471 493
Size: 711 1454 813 2702
Fragments arranged by size:
2702 1454 813 711
BsaBI GATnn'nnATC
Cuts at: 5674 5674
Size: 5680
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2935 3487 3869 4504
Size: 53 83 186 936 1402 552 382 635
Cuts at: 4504 275
Size: 1451
Fragments arranged by size:
1451 1402 936 635 552 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2181 2646 4113 4511 4785 4932 5343 2181
Size: 465 1467 398 274 147 411 2518
Fragments arranged by size:
2518 1467 465 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5455
Fragments arranged by size:
5455 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2153 1186
Size: 967 4713
BseYI C'CCAG_C
Cuts at: 4883 5379 4883
Size: 496 5184
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5107
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3130 3891 4919 1540
Size: 275 186 1129 761 1028 2301
Fragments arranged by size:
2301 1129 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2112 3741 3826 4897 2112
Size: 1629 85 1071 2895
Fragments arranged by size:
2895 1629 1071 85
BsmI GAATG_Cn'
Cuts at: 2089 3191 3284 2089
Size: 1102 93 4485
Fragments arranged by size:
4485 1102 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2112 2467 2479 2999 3741 3826 4897
Size: 399 322 355 12 520 742 85 1071
Cuts at: 4897 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 520 399 355 322 85
12
BspEI T'CCGG_A
Cuts at: 2181 2646 5343 2181
Size: 465 2697 2518
Fragments arranged by size:
2697 2518 465
BspHI T'CATG_A
Cuts at: 3569 5299 3569
Size: 1730 3950
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2757 3035 844
Size: 1913 278 3489
Fragments arranged by size:
3489 1913 278
BsrBI CCG'CTC
Cuts at: 1394 1823 3567 1394
Size: 429 1744 3507
Fragments arranged by size:
3507 1744 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4175 4349 65
Size: 1183 2927 174 1396
Fragments arranged by size:
2927 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4324 4511 1700
Size: 110 2514 187 2869
Fragments arranged by size:
2869 2514 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5680
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5680
BssSI C'ACGA_G
Cuts at: 3740 4752 3740
Size: 1012 4668
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5680
BstBI TT'CG_AA
Cuts at: 4484 4484
Size: 5680
BstEII G'GTnAC_C
Cuts at: 1184 2491 2698 1184
Size: 1307 207 4166
Fragments arranged by size:
4166 1307 207
BstXI CCAn_nnnn'nTGG
Cuts at: 2386 2386
Size: 5680
BstYI r'GATC_y
Cuts at: 1972 2643 3010 3377 3762 3779 5231 5317 5329
Size: 671 367 367 385 17 1452 86 12
Cuts at: 5329 5675 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 671 385 367 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2499 513
Size: 552 939 495 3694
Fragments arranged by size:
3694 939 552 495
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2417 487
Size: 683 56 1191 3750
Fragments arranged by size:
3750 1191 683 56
BtsI GCAGTG_nn'
Cuts at: 2240 3196 3990 4010 2240
Size: 956 794 20 3910
Fragments arranged by size:
3910 956 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5680
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5645
DraI TTT'AAA
Cuts at: 2984 2984
Size: 5680
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4687 809
Size: 507 3371 1802
Fragments arranged by size:
3371 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2302 2376 3127
Size: 54 1425 50 275 18 472 74 751
Cuts at: 3127 4016 5645 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 751 472 275 74 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3127 1537
Size: 275 1315 4090
Fragments arranged by size:
4090 1315 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2252 2526 2810 2864 3610 1353
Size: 899 274 284 54 746 3423
Fragments arranged by size:
3423 899 746 284 274 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4267 4641 4787 387
Size: 1064 138 2678 374 146 1280
Fragments arranged by size:
2678 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3743 4340 5127 5395 1239
Size: 393 2111 597 787 268 1524
Fragments arranged by size:
2111 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2989 3367 2989
Size: 378 5302
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2902 2902
Size: 5680
FspI TGC'GCA
Cuts at: 4186 4186
Size: 5680
HaeII r_GCGC'y
Cuts at: 1678 2829 2938 4827 1678
Size: 1151 109 1889 2531
Fragments arranged by size:
2531 1889 1151 109
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2179 2211 2550 2582 2753
Size: 32 135 32 607 32 339 32 171
Cuts at: 2753 2785 4321 4353 4395 4427 1373
Size: 32 1536 32 42 32 2626
Fragments arranged by size:
2626 1536 607 339 171 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2772 3024 3270 669
Size: 988 281 834 252 246 3079
Fragments arranged by size:
3079 988 834 281 252 246
HindIII A'AGCT_T
Cuts at: 748 2678 748
Size: 1930 3750
HpaI GTT'AAC
Cuts at: 3270 3270
Size: 5680
KasI G'GCGC_C
Cuts at: 2934 2934
Size: 5680
KpnI G_GTAC'C
Cuts at: 2425 3005 2425
Size: 580 5100
MfeI C'AATT_G
Cuts at: 2316 2947 3279 2316
Size: 631 332 4717
Fragments arranged by size:
4717 631 332
MluI A'CGCG_T
Cuts at: 5556 5556
Size: 5680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4794 4978 1305
Size: 3489 184 2007
Fragments arranged by size:
3489 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2304 10
Size: 54 1425 343 472 3386
Fragments arranged by size:
3386 1425 472 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2384 2615 3638 3997 4156 518
Size: 648 1218 231 1023 359 159 2042
Fragments arranged by size:
2042 1218 1023 648 359 231 159
MspA1I CmG'CkG
Cuts at: 1606 3096 3773 4921 5166 1606
Size: 1490 677 1148 245 2120
Fragments arranged by size:
2120 1490 1148 677 245
NarI GG'CG_CC
Cuts at: 2935 2935
Size: 5680
NcoI C'CATG_G
Cuts at: 513 1065 2004 2499 513
Size: 552 939 495 3694
Fragments arranged by size:
3694 939 552 495
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5680
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5680
NotI GC'GGCC_GC
Cuts at: 3127 3127
Size: 5680
NspI r_CATG'y
Cuts at: 1132 1229 3038 4583 1132
Size: 97 1809 1545 2229
Fragments arranged by size:
2229 1809 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5680
PciI A'CATG_T
Cuts at: 4579 4579
Size: 5680
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5680
PmeI GTTT'AAAC
Cuts at: 2984 2984
Size: 5680
PmlI CAC'GTG
Cuts at: 2658 2658
Size: 5680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3749 3781 5262 5294 3749
Size: 32 1481 32 4135
Fragments arranged by size:
4135 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2975 1613
Size: 1362 4318
PsiI TTA'TAA
Cuts at: 3250 3250
Size: 5680
PstI C_TGCA'G
Cuts at: 830 3032 830
Size: 2202 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5680
SalI G'TCGA_C
Cuts at: 1936 2770 3022 1936
Size: 834 252 4594
Fragments arranged by size:
4594 834 252
SapI GCTCTTCn'nnn_
Cuts at: 2252 2810 2252
Size: 558 5122
SbfI CC_TGCA'GG
Cuts at: 3032 3032
Size: 5680
ScaI AGT'ACT
Cuts at: 3928 5640 3928
Size: 1712 3968
SfcI C'TryA_G
Cuts at: 826 1045 3028 4163 4844 5035 826
Size: 219 1983 1135 681 191 1471
Fragments arranged by size:
1983 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2936 2936
Size: 5680
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5680
SmaI CCC'GGG
Cuts at: 1872 3007 1872
Size: 1135 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3784 4685 4947 820
Size: 197 926 1841 901 262 1553
Fragments arranged by size:
1841 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3471 493
Size: 2978 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5680
SphI G_CATG'C
Cuts at: 1132 3038 1132
Size: 1906 3774
SspI AAT'ATT
Cuts at: 5 52 3604 5
Size: 47 3552 2081
Fragments arranged by size:
3552 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5680
StyI C'CwwG_G
Cuts at: 513 1065 2004 2499 3119 513
Size: 552 939 495 620 3074
Fragments arranged by size:
3074 939 620 552 495
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2231 3707 3724 3877 4062 4507 325
Size: 1255 651 1476 17 153 185 445 1498
Fragments arranged by size:
1498 1476 1255 651 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2754 3926 5638
Size: 274 80 33 51 1443 777 1172 1712
Cuts at: 5638 96
Size: 138
Fragments arranged by size:
1712 1443 1172 777 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2780 2812 5640 5672 2780
Size: 32 2828 32 2788
Fragments arranged by size:
2828 2788 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5680
XbaI T'CTAG_A
Cuts at: 3016 3016
Size: 5680
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2629 2629
Size: 5680
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5680
XmaI C'CCGG_G
Cuts at: 1870 3005 1870
Size: 1135 4545
XmnI GAAnn'nnTTC
Cuts at: 2873 3809 2873
Size: 936 4744
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3488 276
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PstI PvuI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SexAI SfiI SrfI SwaI