Restriction map for
pFN21AE1799
(Circular) (Six-base) MAPSORT of: pFN21AE1799.seq Check: 77 from: 1 to: 7234
pFN21AE1799 7234 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5044 278
Size: 53 83 186 936 3508 2468
Fragments arranged by size:
3508 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2639 4577 1937
Size: 702 1938 4594
Fragments arranged by size:
4594 1938 702
Acc65I G'GTAC_C
Cuts at: 4555 4555
Size: 7234
AclI AA'CG_TT
Cuts at: 1563 5361 5734 1563
Size: 3798 373 3063
Fragments arranged by size:
3798 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3078 3993 5297 6681 6949 1632
Size: 1446 915 1304 1384 268 1917
Fragments arranged by size:
1917 1446 1384 1304 915 268
AfeI AGC'GCT
Cuts at: 2669 4115 2669
Size: 1446 5788
AflII C'TTAA_G
Cuts at: 820 1017 4034 820
Size: 197 3017 4020
Fragments arranged by size:
4020 3017 197
AflIII A'CryG_T
Cuts at: 1203 6133 7110 1203
Size: 4930 977 1327
Fragments arranged by size:
4930 1327 977
AgeI A'CCGG_T
Cuts at: 6065 6065
Size: 7234
AhdI GACnn_n'nnGTC
Cuts at: 3420 5963 3420
Size: 2543 4691
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7234
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4408 1184
Size: 3224 4010
AlwNI CAG_nnn'CTG
Cuts at: 2162 3230 4977 6549 2162
Size: 1068 1747 1572 2847
Fragments arranged by size:
2847 1747 1572 1068
ApaLI G'TGCA_C
Cuts at: 5291 6447 5291
Size: 1156 6078
ApoI r'AATT_y
Cuts at: 1493 2600 2807 3044 3356 3710 4132 4543 4769
Size: 1107 207 237 312 354 422 411 226
Cuts at: 4769 4921 7030 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1107 422 411 354 312 237
226 207 152
AseI AT'TA_AT
Cuts at: 160 1032 2793 2888 5788 160
Size: 872 1761 95 2900 1606
Fragments arranged by size:
2900 1761 1606 872 95
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7234
AvaI C'yCGr_G
Cuts at: 1870 1943 4559 1870
Size: 73 2616 4545
Fragments arranged by size:
4545 2616 73
BamHI G'GATC_C
Cuts at: 4564 4931 4564
Size: 367 6867
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4325 4555 6010 618
Size: 325 217 627 2538 230 1455 1842
Fragments arranged by size:
2538 1842 1455 627 325 230 217
BanII G_rGCy'C
Cuts at: 1391 3337 3536 4553 1391
Size: 1946 199 1017 4072
Fragments arranged by size:
4072 1946 1017 199
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2343 2358 928
Size: 971 444 15 5804
Fragments arranged by size:
5804 971 444 15
BbvCI CC'TCA_GC
Cuts at: 4306 4306
Size: 7234
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5425 5459 1903
Size: 34 3488 34 3678
Fragments arranged by size:
3678 3488 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3803 4206 5126 6336 3803
Size: 403 920 1210 4701
Fragments arranged by size:
4701 1210 920 403
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7234
BfrBI ATG'CAT
Cuts at: 3877 4240 3877
Size: 363 6871
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5845 136
Size: 107 122 71 631 605 4173 1525
Fragments arranged by size:
4173 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2681 7229 2681
Size: 4548 2686
BlpI GC'TnA_GC
Cuts at: 2823 4651 2823
Size: 1828 5406
Bme1580I G_kGCm'C
Cuts at: 1790 5295 6451 1790
Size: 3505 1156 2573
Fragments arranged by size:
3505 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7234
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3582 3824 4965
Size: 416 53 83 399 24 2165 242 1141
Cuts at: 4965 5923 442
Size: 958 1753
Fragments arranged by size:
2165 1753 1141 958 416 399 242 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7234
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2061 3674 4012 5894 1239
Size: 822 1613 338 1882 2579
Fragments arranged by size:
2579 1882 1613 822 338
Bpu10I CC'TnA_GC
Cuts at: 4306 4306
Size: 7234
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3231 3682 4176 5359 6224 6522 3231
Size: 451 494 1183 865 298 3943
Fragments arranged by size:
3943 1183 865 494 451 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2371 5897 882
Size: 617 872 3526 2219
Fragments arranged by size:
3526 2219 872 617
BsaAI yAC'GTr
Cuts at: 493 1204 5025 493
Size: 711 3821 2702
Fragments arranged by size:
3821 2702 711
BsaBI GATnn'nnATC
Cuts at: 2686 7228 2686
Size: 4542 2692
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5041 5423 6058 275
Size: 53 83 186 936 3508 382 635 1451
Fragments arranged by size:
3508 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5667 6065 6339 6486 6897 5667
Size: 398 274 147 411 6004
Fragments arranged by size:
6004 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3462 3492 3523 3553 1178
Size: 30 165 30 2059 30 31 30 4859
Fragments arranged by size:
4859 2059 165 31 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2062 2264 2394 2949 3135 3662 1186
Size: 876 202 130 555 186 527 4758
Fragments arranged by size:
4758 876 555 527 202 186 130
BseYI C'CCAG_C
Cuts at: 4116 6437 6933 4116
Size: 2321 496 4417
Fragments arranged by size:
4417 2321 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3168 1163
Size: 573 1432 5229
Fragments arranged by size:
5229 1432 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4684 5445 6473 1540
Size: 275 186 2683 761 1028 2301
Fragments arranged by size:
2683 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3660 4192 5295 5380 6451 3660
Size: 532 1103 85 1071 4443
Fragments arranged by size:
4443 1103 1071 532 85
BsmI GAATG_Cn'
Cuts at: 2123 3575 4063 4745 4838 2123
Size: 1452 488 682 93 4519
Fragments arranged by size:
4519 1452 682 488 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3337 3536 3660 4192 4553 5295 5380
Size: 399 1547 199 124 532 361 742 85
Cuts at: 5380 6451 1391
Size: 1071 2174
Fragments arranged by size:
2174 1547 1071 742 532 399 361 199
124 85
BspEI T'CCGG_A
Cuts at: 6897 6897
Size: 7234
BspHI T'CATG_A
Cuts at: 5123 6853 5123
Size: 1730 5504
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4589 844
Size: 3745 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5121 1394
Size: 429 3298 3507
Fragments arranged by size:
3507 3298 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2128 2320 3370 3430 3679 3808 5729
Size: 1183 880 192 1050 60 249 129 1921
Cuts at: 5729 5903 65
Size: 174 1396
Fragments arranged by size:
1921 1396 1183 1050 880 249 192 174
129 60
BsrFI r'CCGG_y
Cuts at: 1700 1810 3375 5878 6065 1700
Size: 110 1565 2503 187 2869
Fragments arranged by size:
2869 2503 1565 187 110
BsrGI T'GTAC_A
Cuts at: 96 3792 4069 96
Size: 3696 277 3261
Fragments arranged by size:
3696 3261 277
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7234
BssSI C'ACGA_G
Cuts at: 5294 6306 5294
Size: 1012 6222
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3636 794
Size: 2842 4392
BstBI TT'CG_AA
Cuts at: 6038 6038
Size: 7234
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7234
BstYI r'GATC_y
Cuts at: 1972 2681 3602 4564 4931 5316 5333 6785 6871
Size: 709 921 962 367 385 17 1452 86
Cuts at: 6871 6883 7229 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 962 921 709 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3022 3022
Size: 7234
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6682
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6495
Fragments arranged by size:
6495 683 56
BtsI GCAGTG_nn'
Cuts at: 4104 4750 5544 5564 4104
Size: 646 794 20 5774
Fragments arranged by size:
5774 794 646 20
ClaI AT'CG_AT
Cuts at: 1554 2394 1554
Size: 840 6394
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7199
DraI TTT'AAA
Cuts at: 3084 4538 3084
Size: 1454 5780
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6241 809
Size: 507 4925 1802
Fragments arranged by size:
4925 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2449 3834 4681
Size: 54 1425 50 275 18 619 1385 847
Cuts at: 4681 5570 7199 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1385 889 847 619 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4681 1537
Size: 275 2869 4090
Fragments arranged by size:
4090 2869 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3336 5164 1353
Size: 1983 1828 3423
Fragments arranged by size:
3423 1983 1828
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5821 6195 6341 387
Size: 1064 138 4232 374 146 1280
Fragments arranged by size:
4232 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2061 3078 3674 3993 4012 5297 5894
Size: 393 429 1017 596 319 19 1285 597
Cuts at: 5894 6681 6949 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1285 1017 787 597 596 429 393
319 268 19
EcoNI CCTnn'n_nnAGG
Cuts at: 2232 2232
Size: 7234
EcoO109I rG'GnC_Cy
Cuts at: 3414 4004 3414
Size: 590 6644
EcoRI G'AATT_C
Cuts at: 3710 4132 4543 4921 3710
Size: 422 411 378 6023
Fragments arranged by size:
6023 422 411 378
EcoRV GAT'ATC
Cuts at: 2392 2392
Size: 7234
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2397 2689 2397
Size: 292 6942
FspI TGC'GCA
Cuts at: 5740 5740
Size: 7234
HaeII r_GCGC'y
Cuts at: 1678 2671 4117 4470 6381 1678
Size: 993 1446 353 1911 2531
Fragments arranged by size:
2531 1911 1446 993 353
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2128 2160 2896 2928 3406
Size: 32 135 32 556 32 736 32 478
Cuts at: 3406 3438 4204 4236 5875 5907 5949 5981 1373
Size: 32 766 32 1639 32 42 32 2626
Fragments arranged by size:
2626 1639 766 736 556 478 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2083 2640 4089 4578 4824 669
Size: 988 281 145 557 1449 489 246 3079
Fragments arranged by size:
3079 1449 988 557 489 281 246 145
HindIII A'AGCT_T
Cuts at: 748 2017 4317 748
Size: 1269 2300 3665
Fragments arranged by size:
3665 2300 1269
HpaI GTT'AAC
Cuts at: 4089 4824 4089
Size: 735 6499
KpnI G_GTAC'C
Cuts at: 4559 4559
Size: 7234
MfeI C'AATT_G
Cuts at: 4833 4833
Size: 7234
MluI A'CGCG_T
Cuts at: 7110 7110
Size: 7234
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2176 2267 2642 3491 3592 3728 6348 6532
Size: 871 91 375 849 101 136 2620 184
Cuts at: 6532 1305
Size: 2007
Fragments arranged by size:
2620 2007 871 849 375 184 136 101
91
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2451 3836 10
Size: 54 1425 343 619 1385 3408
Fragments arranged by size:
3408 1425 1385 619 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2030 2721 4390 4418 5192 5551
Size: 648 843 21 691 1669 28 774 359
Cuts at: 5551 5710 518
Size: 159 2042
Fragments arranged by size:
2042 1669 843 774 691 648 359 159
28 21
MspA1I CmG'CkG
Cuts at: 1606 4065 4290 4310 4650 5327 6475 6720 1606
Size: 2459 225 20 340 677 1148 245 2120
Fragments arranged by size:
2459 2120 1148 677 340 245 225 20
NaeI GCC'GGC
Cuts at: 3377 3377
Size: 7234
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6682
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7234
NgoMIV G'CCGG_C
Cuts at: 3375 3375
Size: 7234
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7234
NotI GC'GGCC_GC
Cuts at: 4681 4681
Size: 7234
NsiI A_TGCA'T
Cuts at: 3879 4242 3879
Size: 363 6871
NspI r_CATG'y
Cuts at: 1132 1229 3994 4244 4592 6137 1132
Size: 97 2765 250 348 1545 2229
Fragments arranged by size:
2765 2229 1545 348 250 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7234
PciI A'CATG_T
Cuts at: 6133 6133
Size: 7234
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2755 1108
Size: 1647 5587
PmeI GTTT'AAAC
Cuts at: 4538 4538
Size: 7234
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5303 5335 6816 6848 5303
Size: 32 1481 32 5689
Fragments arranged by size:
5689 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3414 4004 3414
Size: 590 6644
PshAI GACnn'nnGTC
Cuts at: 1613 3366 1613
Size: 1753 5481
PsiI TTA'TAA
Cuts at: 4804 4804
Size: 7234
PstI C_TGCA'G
Cuts at: 830 4131 4586 830
Size: 3301 455 3478
Fragments arranged by size:
3478 3301 455
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7234
PvuII CAG'CTG
Cuts at: 4290 4310 4290
Size: 20 7214
SalI G'TCGA_C
Cuts at: 1936 2638 4576 1936
Size: 702 1938 4594
Fragments arranged by size:
4594 1938 702
SanDI GG'GwC_CC
Cuts at: 3414 3414
Size: 7234
SbfI CC_TGCA'GG
Cuts at: 4586 4586
Size: 7234
ScaI AGT'ACT
Cuts at: 2614 5482 7194 2614
Size: 2868 1712 2654
Fragments arranged by size:
2868 2654 1712
SfcI C'TryA_G
Cuts at: 826 1045 2533 2869 4127 4582 5717 6398 6589
Size: 219 1488 336 1258 455 1135 681 191
Cuts at: 6589 826
Size: 1471
Fragments arranged by size:
1488 1471 1258 1135 681 455 336 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7234
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7234
SmaI CCC'GGG
Cuts at: 1872 4561 1872
Size: 2689 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3246 3661 4034 4191 5338 6239
Size: 197 926 1303 415 373 157 1147 901
Cuts at: 6239 6501 820
Size: 262 1553
Fragments arranged by size:
1553 1303 1147 926 901 415 373 262
197 157
SnaBI TAC'GTA
Cuts at: 493 5025 493
Size: 4532 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7234
SphI G_CATG'C
Cuts at: 1132 4592 1132
Size: 3460 3774
SspI AAT'ATT
Cuts at: 5 52 5158 5
Size: 47 5106 2081
Fragments arranged by size:
5106 2081 47
StuI AGG'CCT
Cuts at: 1517 4513 1517
Size: 2996 4238
StyI C'CwwG_G
Cuts at: 513 1065 2108 3544 4176 4673 513
Size: 552 1043 1436 632 497 3074
Fragments arranged by size:
3074 1436 1043 632 552 497
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5261 5278 5431 5616 6061 325
Size: 1255 3681 17 153 185 445 1498
Fragments arranged by size:
3681 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2612 3792 3907
Size: 274 80 33 51 1443 635 1180 115
Cuts at: 3907 4069 4292 5480 7192 96
Size: 162 223 1188 1712 138
Fragments arranged by size:
1712 1443 1188 1180 635 274 223 162
138 115 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3523 3555 3816 3848 7194 7226 3523
Size: 32 261 32 3346 32 3531
Fragments arranged by size:
3531 3346 261 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4447 1316
Size: 3131 4103
XbaI T'CTAG_A
Cuts at: 4570 4570
Size: 7234
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2192 2192
Size: 7234
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7234
XmaI C'CCGG_G
Cuts at: 1870 4559 1870
Size: 2689 4545
XmnI GAAnn'nnTTC
Cuts at: 5363 5363
Size: 7234
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5042 276
Size: 53 83 186 936 3508 2468
Fragments arranged by size:
3508 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI FseI FspAI
KasI NarI NruI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SapI SexAI SfiI SfoI
SrfI SwaI