Restriction map for
pFN21AE1795
(Circular) (Six-base) MAPSORT of: pFN21AE1795.seq Check: 6504 from: 1 to: 5644
pFN21AE1795 5644 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3454 278
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2987 1937
Size: 1050 4594
Acc65I G'GTAC_C
Cuts at: 2965 2965
Size: 5644
AclI AA'CG_TT
Cuts at: 1563 3771 4144 1563
Size: 2208 373 3063
Fragments arranged by size:
3063 2208 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2050 2191 3707 5091 5359 1632
Size: 418 141 1516 1384 268 1917
Fragments arranged by size:
1917 1516 1384 418 268 141
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5447
AflIII A'CryG_T
Cuts at: 1203 2188 4543 5520 1203
Size: 985 2355 977 1327
Fragments arranged by size:
2355 1327 985 977
AgeI A'CCGG_T
Cuts at: 4475 4475
Size: 5644
AhdI GACnn_n'nnGTC
Cuts at: 4373 4373
Size: 5644
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5644
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2335 1184
Size: 1151 4493
AlwNI CAG_nnn'CTG
Cuts at: 2285 2333 3387 4959 2285
Size: 48 1054 1572 2970
Fragments arranged by size:
2970 1572 1054 48
ApaLI G'TGCA_C
Cuts at: 3701 4857 3701
Size: 1156 4488
ApoI r'AATT_y
Cuts at: 1493 2564 2576 2662 2953 3179 3331 5440 1493
Size: 1071 12 86 291 226 152 2109 1697
Fragments arranged by size:
2109 1697 1071 291 226 152 86 12
AseI AT'TA_AT
Cuts at: 160 1032 4198 160
Size: 872 3166 1606
Fragments arranged by size:
3166 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
AvaI C'yCGr_G
Cuts at: 1870 1943 2969 1870
Size: 73 1026 4545
Fragments arranged by size:
4545 1026 73
BamHI G'GATC_C
Cuts at: 2974 3341 2974
Size: 367 5277
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2019 2965 4420 618
Size: 325 217 627 232 946 1455 1842
Fragments arranged by size:
1842 1455 946 627 325 232 217
BanII G_rGCy'C
Cuts at: 1391 2963 1391
Size: 1572 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2144 928
Size: 971 245 4428
Fragments arranged by size:
4428 971 245
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3835 3869 1903
Size: 34 1898 34 3678
Fragments arranged by size:
3678 1898 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3536 4746 3536
Size: 1210 4434
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5644
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4255 136
Size: 107 122 71 631 605 2583 1525
Fragments arranged by size:
2583 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2171 5639 2171
Size: 3468 2176
BlpI GC'TnA_GC
Cuts at: 2091 3061 2091
Size: 970 4674
Bme1580I G_kGCm'C
Cuts at: 1790 2196 3705 4861 1790
Size: 406 1509 1156 2573
Fragments arranged by size:
2573 1509 1156 406
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5644
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2147 3375 4333
Size: 416 53 83 399 24 730 1228 958
Cuts at: 4333 442
Size: 1753
Fragments arranged by size:
1753 1228 958 730 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5644
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2682 2682
Size: 5644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2063 2443 2577 4304 1239
Size: 824 380 134 1727 2579
Fragments arranged by size:
2579 1727 824 380 134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2677 3769 4634 4932 2677
Size: 1092 865 298 3389
Fragments arranged by size:
3389 1092 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4307 882
Size: 617 2808 2219
Fragments arranged by size:
2808 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2191 3435 493
Size: 711 987 1244 2702
Fragments arranged by size:
2702 1244 987 711
BsaBI GATnn'nnATC
Cuts at: 5638 5638
Size: 5644
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3451 3833 4468 275
Size: 53 83 186 936 1918 382 635 1451
Fragments arranged by size:
1918 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2864 4077 4475 4749 4896 5307 2864
Size: 1213 398 274 147 411 3201
Fragments arranged by size:
3201 1213 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5419
Fragments arranged by size:
5419 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5644
BseYI C'CCAG_C
Cuts at: 4847 5343 4847
Size: 496 5148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2218 1163
Size: 573 482 4589
Fragments arranged by size:
4589 573 482
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3094 3855 4883 1540
Size: 275 186 1093 761 1028 2301
Fragments arranged by size:
2301 1093 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3705 3790 4861 3705
Size: 85 1071 4488
Fragments arranged by size:
4488 1071 85
BsmI GAATG_Cn'
Cuts at: 2557 2650 3155 3248 2557
Size: 93 505 93 4953
Fragments arranged by size:
4953 505 93 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2196 2963 3705 3790 4861 1391
Size: 399 406 767 742 85 1071 2174
Fragments arranged by size:
2174 1071 767 742 406 399 85
BspEI T'CCGG_A
Cuts at: 5307 5307
Size: 5644
BspHI T'CATG_A
Cuts at: 3533 5263 3533
Size: 1730 3914
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2240 2470 2999 844
Size: 1396 230 529 3489
Fragments arranged by size:
3489 1396 529 230
BsrBI CCG'CTC
Cuts at: 1394 1823 3531 1394
Size: 429 1708 3507
Fragments arranged by size:
3507 1708 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2887 4139 4313 65
Size: 1183 1639 1252 174 1396
Fragments arranged by size:
1639 1396 1252 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4288 4475 1700
Size: 110 2478 187 2869
Fragments arranged by size:
2869 2478 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5644
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5644
BssSI C'ACGA_G
Cuts at: 3704 4716 3704
Size: 1012 4632
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2285 794
Size: 1491 4153
BstBI TT'CG_AA
Cuts at: 4448 4448
Size: 5644
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5644
BstYI r'GATC_y
Cuts at: 1972 2171 2974 3341 3726 3743 5195 5281 5293
Size: 199 803 367 385 17 1452 86 12
Cuts at: 5293 5639 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 803 385 367 346 199 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5092
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4905
Fragments arranged by size:
4905 683 56
BtsI GCAGTG_nn'
Cuts at: 3160 3954 3974 3160
Size: 794 20 4830
Fragments arranged by size:
4830 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5644
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5609
DraI TTT'AAA
Cuts at: 2922 2948 2922
Size: 26 5618
DraIII CAC_nnn'GTG
Cuts at: 2891 2891
Size: 5644
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4651 809
Size: 507 3335 1802
Fragments arranged by size:
3335 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3091 3980 5609
Size: 54 1425 50 275 18 1261 889 1629
Cuts at: 5609 8
Size: 43
Fragments arranged by size:
1629 1425 1261 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3091 1537
Size: 275 1279 4090
Fragments arranged by size:
4090 1279 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3574 1353
Size: 2221 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4231 4605 4751 387
Size: 1064 138 2642 374 146 1280
Fragments arranged by size:
2642 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2050 2063 2191 2443 2577 3707 4304
Size: 393 418 13 128 252 134 1130 597
Cuts at: 4304 5091 5359 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1130 787 597 418 393 268 252
134 128 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2447 2447
Size: 5644
EcoO109I rG'GnC_Cy
Cuts at: 2032 2061 2459 2032
Size: 29 398 5217
Fragments arranged by size:
5217 398 29
EcoRI G'AATT_C
Cuts at: 2662 2953 3331 2662
Size: 291 378 4975
Fragments arranged by size:
4975 378 291
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2701 2701
Size: 5644
FspI TGC'GCA
Cuts at: 4150 4150
Size: 5644
HaeII r_GCGC'y
Cuts at: 1678 2802 4791 1678
Size: 1124 1989 2531
Fragments arranged by size:
2531 1989 1124
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4285 4317 4359 4391 1373
Size: 32 135 32 2713 32 42 32 2626
Fragments arranged by size:
2713 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2988 3234 669
Size: 988 281 1050 246 3079
Fragments arranged by size:
3079 1050 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5644
HpaI GTT'AAC
Cuts at: 3234 3234
Size: 5644
KpnI G_GTAC'C
Cuts at: 2969 2969
Size: 5644
MfeI C'AATT_G
Cuts at: 3243 3243
Size: 5644
MluI A'CGCG_T
Cuts at: 5520 5520
Size: 5644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4758 4942 1305
Size: 3453 184 2007
Fragments arranged by size:
3453 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3822
Fragments arranged by size:
3822 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3602 3961 4120 518
Size: 648 2436 359 159 2042
Fragments arranged by size:
2436 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2041 2734 3060 3737 4885 5130 1606
Size: 435 693 326 677 1148 245 2120
Fragments arranged by size:
2120 1148 693 677 435 326 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5092
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5644
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5644
NotI GC'GGCC_GC
Cuts at: 3091 3091
Size: 5644
NspI r_CATG'y
Cuts at: 1132 1229 3002 4547 1132
Size: 97 1773 1545 2229
Fragments arranged by size:
2229 1773 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5644
PciI A'CATG_T
Cuts at: 4543 4543
Size: 5644
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5644
PmeI GTTT'AAAC
Cuts at: 2948 2948
Size: 5644
PmlI CAC'GTG
Cuts at: 2191 2191
Size: 5644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3713 3745 5226 5258 3713
Size: 32 1481 32 4099
Fragments arranged by size:
4099 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2061 2459 2061
Size: 398 5246
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5644
PsiI TTA'TAA
Cuts at: 3214 3214
Size: 5644
PstI C_TGCA'G
Cuts at: 830 2281 2996 830
Size: 1451 715 3478
Fragments arranged by size:
3478 1451 715
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5644
PvuII CAG'CTG
Cuts at: 2734 2734
Size: 5644
SalI G'TCGA_C
Cuts at: 1936 2986 1936
Size: 1050 4594
SbfI CC_TGCA'GG
Cuts at: 2996 2996
Size: 5644
ScaI AGT'ACT
Cuts at: 3892 5604 3892
Size: 1712 3932
SfcI C'TryA_G
Cuts at: 826 1045 2277 2992 4127 4808 4999 826
Size: 219 1232 715 1135 681 191 1471
Fragments arranged by size:
1471 1232 1135 715 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5644
SmaI CCC'GGG
Cuts at: 1872 2971 1872
Size: 1099 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2656 3748 4649 4911 820
Size: 197 926 713 1092 901 262 1553
Fragments arranged by size:
1553 1092 926 901 713 262 197
SnaBI TAC'GTA
Cuts at: 493 3435 493
Size: 2942 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5644
SphI G_CATG'C
Cuts at: 1132 3002 1132
Size: 1870 3774
SspI AAT'ATT
Cuts at: 5 52 2587 3568 5
Size: 47 2535 981 2081
Fragments arranged by size:
2535 2081 981 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5644
StyI C'CwwG_G
Cuts at: 513 1065 2751 3083 513
Size: 552 1686 332 3074
Fragments arranged by size:
3074 1686 552 332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3671 3688 3841 4026 4471 325
Size: 1255 2091 17 153 185 445 1498
Fragments arranged by size:
2091 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2642 3890 5602
Size: 274 80 33 51 1443 665 1248 1712
Cuts at: 5602 96
Size: 138
Fragments arranged by size:
1712 1443 1248 665 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5604 5636 5604
Size: 32 5612
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5644
XbaI T'CTAG_A
Cuts at: 2980 2980
Size: 5644
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5644
XmaI C'CCGG_G
Cuts at: 1870 2969 1870
Size: 1099 4545
XmnI GAAnn'nnTTC
Cuts at: 3773 3773
Size: 5644
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3452 276
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI