Restriction map for
pFN21AE1789
(Circular) (Six-base) MAPSORT of: pFN21AE1789.seq Check: 1204 from: 1 to: 5593
pFN21AE1789 5593 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3403 278
Size: 53 83 186 936 1867 2468
Fragments arranged by size:
2468 1867 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2832 2936 1937
Size: 895 104 4594
Fragments arranged by size:
4594 895 104
Acc65I G'GTAC_C
Cuts at: 2914 2914
Size: 5593
AclI AA'CG_TT
Cuts at: 1563 3720 4093 1563
Size: 2157 373 3063
Fragments arranged by size:
3063 2157 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2904 3656 5040 5308 1632
Size: 1272 752 1384 268 1917
Fragments arranged by size:
1917 1384 1272 752 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5396
AflIII A'CryG_T
Cuts at: 1203 4492 5469 1203
Size: 3289 977 1327
Fragments arranged by size:
3289 1327 977
AgeI A'CCGG_T
Cuts at: 4424 4424
Size: 5593
AhdI GACnn_n'nnGTC
Cuts at: 4322 4322
Size: 5593
AleI CACnn'nnGTG
Cuts at: 1166 2368 1166
Size: 1202 4391
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5593
AlwNI CAG_nnn'CTG
Cuts at: 3336 4908 3336
Size: 1572 4021
ApaLI G'TGCA_C
Cuts at: 3650 4806 3650
Size: 1156 4437
ApoI r'AATT_y
Cuts at: 1493 2555 2757 2902 3128 3280 5389 1493
Size: 1062 202 145 226 152 2109 1697
Fragments arranged by size:
2109 1697 1062 226 202 152 145
AseI AT'TA_AT
Cuts at: 160 1032 4147 160
Size: 872 3115 1606
Fragments arranged by size:
3115 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5593
AvaI C'yCGr_G
Cuts at: 1870 1943 2162 2918 1870
Size: 73 219 756 4545
Fragments arranged by size:
4545 756 219 73
BamHI G'GATC_C
Cuts at: 2923 3290 2923
Size: 367 5226
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2914 4369 618
Size: 325 217 627 1127 1455 1842
Fragments arranged by size:
1842 1455 1127 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2063 2397 2882 2912 1391
Size: 672 334 485 30 4072
Fragments arranged by size:
4072 672 485 334 30
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2806 928
Size: 971 907 3715
Fragments arranged by size:
3715 971 907
BbvCI CC'TCA_GC
Cuts at: 2231 2231
Size: 5593
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3784 3818 1903
Size: 34 1847 34 3678
Fragments arranged by size:
3678 1847 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2782 3485 4695 2782
Size: 703 1210 3680
Fragments arranged by size:
3680 1210 703
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5593
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4204 136
Size: 107 122 71 631 605 2532 1525
Fragments arranged by size:
2532 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2821 5588 2821
Size: 2767 2826
BlpI GC'TnA_GC
Cuts at: 3010 3010
Size: 5593
Bme1580I G_kGCm'C
Cuts at: 1790 3654 4810 1790
Size: 1864 1156 2573
Fragments arranged by size:
2573 1864 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5593
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2342 2520 3324
Size: 416 53 83 399 24 925 178 804
Cuts at: 3324 4282 442
Size: 958 1753
Fragments arranged by size:
1753 958 925 804 416 399 178 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5593
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4253 1239
Size: 3014 2579
Bpu10I CC'TnA_GC
Cuts at: 2062 2231 2498 2062
Size: 169 267 5157
Fragments arranged by size:
5157 267 169
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2411 3718 4583 4881 2411
Size: 1307 865 298 3123
Fragments arranged by size:
3123 1307 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4256 882
Size: 617 2757 2219
Fragments arranged by size:
2757 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3384 493
Size: 711 2180 2702
Fragments arranged by size:
2702 2180 711
BsaBI GATnn'nnATC
Cuts at: 5587 5587
Size: 5593
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3400 3782 4417 275
Size: 53 83 186 936 1867 382 635 1451
Fragments arranged by size:
1867 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2139 4026 4424 4698 4845 5256 2139
Size: 1887 398 274 147 411 2476
Fragments arranged by size:
2476 1887 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5368
Fragments arranged by size:
5368 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5593
BseYI C'CCAG_C
Cuts at: 2053 2079 2263 4796 5292 2053
Size: 26 184 2533 496 2354
Fragments arranged by size:
2533 2354 496 184 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2392 2724 1163
Size: 573 656 332 4032
Fragments arranged by size:
4032 656 573 332
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3043 3804 4832 1540
Size: 275 186 1042 761 1028 2301
Fragments arranged by size:
2301 1042 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2397 2596 3654 3739 4810 2397
Size: 199 1058 85 1071 3180
Fragments arranged by size:
3180 1071 1058 199 85
BsmI GAATG_Cn'
Cuts at: 3104 3197 3104
Size: 93 5500
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2063 2397 2596 2882 2912 3654 3739
Size: 399 273 334 199 286 30 742 85
Cuts at: 3739 4810 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 334 286 273 199
85 30
BspEI T'CCGG_A
Cuts at: 5256 5256
Size: 5593
BspHI T'CATG_A
Cuts at: 2115 2857 3482 5212 2115
Size: 742 625 1730 2496
Fragments arranged by size:
2496 1730 742 625
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2948 844
Size: 2104 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2169 3480 1394
Size: 429 346 1311 3507
Fragments arranged by size:
3507 1311 429 346
BsrDI GCAATG_nn'
Cuts at: 65 1248 2496 4088 4262 65
Size: 1183 1248 1592 174 1396
Fragments arranged by size:
1592 1396 1248 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4237 4424 1700
Size: 110 2427 187 2869
Fragments arranged by size:
2869 2427 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5593
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5593
BssSI C'ACGA_G
Cuts at: 3653 4665 3653
Size: 1012 4581
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5593
BstBI TT'CG_AA
Cuts at: 2481 2569 4397 2481
Size: 88 1828 3677
Fragments arranged by size:
3677 1828 88
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5593
BstXI CCAn_nnnn'nTGG
Cuts at: 2303 2303
Size: 5593
BstYI r'GATC_y
Cuts at: 1972 2821 2923 3290 3675 3692 5144 5230 5242
Size: 849 102 367 385 17 1452 86 12
Cuts at: 5242 5588 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 849 385 367 346 102 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2452 513
Size: 552 939 448 3654
Fragments arranged by size:
3654 939 552 448
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4854
Fragments arranged by size:
4854 683 56
BtsI GCAGTG_nn'
Cuts at: 3109 3903 3923 3109
Size: 794 20 4779
Fragments arranged by size:
4779 794 20
ClaI AT'CG_AT
Cuts at: 1554 2779 1554
Size: 1225 4368
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5558
DraI TTT'AAA
Cuts at: 2897 2897
Size: 5593
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4600 809
Size: 507 3284 1802
Fragments arranged by size:
3284 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 2310 3040
Size: 54 1425 50 275 18 186 294 730
Cuts at: 3040 3929 5558 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 730 294 275 186 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3040 1537
Size: 275 1228 4090
Fragments arranged by size:
4090 1228 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2601 3523 1353
Size: 1248 922 3423
Fragments arranged by size:
3423 1248 922
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2105 4180 4554 4700 387
Size: 1064 138 516 2075 374 146 1280
Fragments arranged by size:
2075 1280 1064 516 374 146 138
EcoICRI GAG'CTC
Cuts at: 2395 2395
Size: 5593
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2904 3656 4253 5040 5308 1239
Size: 393 1272 752 597 787 268 1524
Fragments arranged by size:
1524 1272 787 752 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2050 2325 2416 2050
Size: 275 91 5227
Fragments arranged by size:
5227 275 91
EcoRI G'AATT_C
Cuts at: 2757 2902 3280 2757
Size: 145 378 5070
Fragments arranged by size:
5070 378 145
FspI TGC'GCA
Cuts at: 4099 4099
Size: 5593
HaeII r_GCGC'y
Cuts at: 1678 4740 1678
Size: 3062 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4234 4266 4308 4340 1373
Size: 32 135 32 2662 32 42 32 2626
Fragments arranged by size:
2662 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2386 2937 3183 669
Size: 988 281 448 551 246 3079
Fragments arranged by size:
3079 988 551 448 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5593
HpaI GTT'AAC
Cuts at: 3183 3183
Size: 5593
KpnI G_GTAC'C
Cuts at: 2918 2918
Size: 5593
MfeI C'AATT_G
Cuts at: 3192 3192
Size: 5593
MluI A'CGCG_T
Cuts at: 5469 5469
Size: 5593
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2081 4707 4891 1305
Size: 776 2626 184 2007
Fragments arranged by size:
2626 2007 776 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3771
Fragments arranged by size:
3771 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2368 3551 3910 4069 518
Size: 648 1202 1183 359 159 2042
Fragments arranged by size:
2042 1202 1183 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2057 2404 2426 2509 2738 3009 3686 4834
Size: 451 347 22 83 229 271 677 1148
Cuts at: 4834 5079 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 451 347 271 245 229
83 22
NcoI C'CATG_G
Cuts at: 513 1065 2004 2452 513
Size: 552 939 448 3654
Fragments arranged by size:
3654 939 552 448
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5593
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5593
NotI GC'GGCC_GC
Cuts at: 3040 3040
Size: 5593
NspI r_CATG'y
Cuts at: 1132 1229 2951 4496 1132
Size: 97 1722 1545 2229
Fragments arranged by size:
2229 1722 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5593
PciI A'CATG_T
Cuts at: 4492 4492
Size: 5593
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5593
PmeI GTTT'AAAC
Cuts at: 2897 2897
Size: 5593
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3662 3694 5175 5207 3662
Size: 32 1481 32 4048
Fragments arranged by size:
4048 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2050 2325 2416 2050
Size: 275 91 5227
Fragments arranged by size:
5227 275 91
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5593
PsiI TTA'TAA
Cuts at: 3163 3163
Size: 5593
PstI C_TGCA'G
Cuts at: 830 2945 830
Size: 2115 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5593
PvuII CAG'CTG
Cuts at: 2509 2738 2509
Size: 229 5364
SacI G_AGCT'C
Cuts at: 2397 2397
Size: 5593
SalI G'TCGA_C
Cuts at: 1936 2935 1936
Size: 999 4594
SanDI GG'GwC_CC
Cuts at: 2050 2325 2050
Size: 275 5318
SapI GCTCTTCn'nnn_
Cuts at: 2601 2601
Size: 5593
SbfI CC_TGCA'GG
Cuts at: 2945 2945
Size: 5593
ScaI AGT'ACT
Cuts at: 2730 3841 5553 2730
Size: 1111 1712 2770
Fragments arranged by size:
2770 1712 1111
SfcI C'TryA_G
Cuts at: 826 1045 2794 2941 4076 4757 4948 826
Size: 219 1749 147 1135 681 191 1471
Fragments arranged by size:
1749 1471 1135 681 219 191 147
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5593
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5593
SmaI CCC'GGG
Cuts at: 1872 2164 2920 1872
Size: 292 756 4545
Fragments arranged by size:
4545 756 292
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2390 3697 4598 4860 820
Size: 197 926 447 1307 901 262 1553
Fragments arranged by size:
1553 1307 926 901 447 262 197
SnaBI TAC'GTA
Cuts at: 493 3384 493
Size: 2891 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5593
SphI G_CATG'C
Cuts at: 1132 2951 1132
Size: 1819 3774
SspI AAT'ATT
Cuts at: 5 52 2574 2693 3517 5
Size: 47 2522 119 824 2081
Fragments arranged by size:
2522 2081 824 119 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5593
StyI C'CwwG_G
Cuts at: 513 1065 2004 2452 3032 513
Size: 552 939 448 580 3074
Fragments arranged by size:
3074 939 580 552 448
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2255 2422 3620 3637 3790 3975 4420
Size: 1255 675 167 1198 17 153 185 445
Cuts at: 4420 325
Size: 1498
Fragments arranged by size:
1498 1255 1198 675 445 185 167 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2279 2728 3839
Size: 274 80 33 51 1443 302 449 1111
Cuts at: 3839 5551 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1111 449 302 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5553 5585 5553
Size: 32 5561
Tth111I GACn'n_nGTC
Cuts at: 1316 2432 1316
Size: 1116 4477
XbaI T'CTAG_A
Cuts at: 2929 2929
Size: 5593
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5593
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5593
XmaI C'CCGG_G
Cuts at: 1870 2162 2918 1870
Size: 292 756 4545
Fragments arranged by size:
4545 756 292
XmnI GAAnn'nnTTC
Cuts at: 3722 3722
Size: 5593
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3401 276
Size: 53 83 186 936 1867 2468
Fragments arranged by size:
2468 1867 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacII SexAI SfiI SfoI SrfI SwaI