Restriction map for
pFN21AE1779
(Circular) (Six-base) MAPSORT of: pFN21AE1779.seq Check: 5787 from: 1 to: 5569
pFN21AE1779 5569 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3379 278
Size: 53 83 186 936 1843 2468
Fragments arranged by size:
2468 1843 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2912 1937
Size: 975 4594
Acc65I G'GTAC_C
Cuts at: 2890 2890
Size: 5569
AclI AA'CG_TT
Cuts at: 1563 3696 4069 1563
Size: 2133 373 3063
Fragments arranged by size:
3063 2133 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2381 2421 3632 5016 5284 1632
Size: 749 40 1211 1384 268 1917
Fragments arranged by size:
1917 1384 1211 749 268 40
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5372
AflIII A'CryG_T
Cuts at: 1203 4468 5445 1203
Size: 3265 977 1327
Fragments arranged by size:
3265 1327 977
AgeI A'CCGG_T
Cuts at: 4400 4400
Size: 5569
AhdI GACnn_n'nnGTC
Cuts at: 4298 4298
Size: 5569
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5569
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5569
AlwNI CAG_nnn'CTG
Cuts at: 3312 4884 3312
Size: 1572 3997
ApaLI G'TGCA_C
Cuts at: 3626 4782 3626
Size: 1156 4413
ApoI r'AATT_y
Cuts at: 1493 2457 2772 2878 3104 3256 5365 1493
Size: 964 315 106 226 152 2109 1697
Fragments arranged by size:
2109 1697 964 315 226 152 106
AseI AT'TA_AT
Cuts at: 160 1032 4123 160
Size: 872 3091 1606
Fragments arranged by size:
3091 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5569
AvaI C'yCGr_G
Cuts at: 1870 1943 2258 2894 1870
Size: 73 315 636 4545
Fragments arranged by size:
4545 636 315 73
BamHI G'GATC_C
Cuts at: 2899 3266 2899
Size: 367 5202
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2890 4345 618
Size: 325 217 627 1103 1455 1842
Fragments arranged by size:
1842 1455 1103 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2595 2888 1391
Size: 1204 293 4072
Fragments arranged by size:
4072 1204 293
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4598
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3760 3794 1903
Size: 34 1823 34 3678
Fragments arranged by size:
3678 1823 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3461 4671 3461
Size: 1210 4359
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5569
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4180 136
Size: 107 122 71 631 605 2508 1525
Fragments arranged by size:
2508 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5564 5564
Size: 5569
BlpI GC'TnA_GC
Cuts at: 2986 2986
Size: 5569
Bme1580I G_kGCm'C
Cuts at: 1790 2342 3630 4786 1790
Size: 552 1288 1156 2573
Fragments arranged by size:
2573 1288 1156 552
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5569
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3300 4258 442
Size: 416 53 83 399 24 1883 958 1753
Fragments arranged by size:
1883 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5569
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2520 4229 1239
Size: 1281 1709 2579
Fragments arranged by size:
2579 1709 1281
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3694 4559 4857 3694
Size: 865 298 4406
Fragments arranged by size:
4406 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4232 882
Size: 617 2733 2219
Fragments arranged by size:
2733 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2116 3360 493
Size: 711 912 1244 2702
Fragments arranged by size:
2702 1244 912 711
BsaBI GATnn'nnATC
Cuts at: 5563 5563
Size: 5569
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3376 3758 4393 275
Size: 53 83 186 936 1843 382 635 1451
Fragments arranged by size:
1843 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2271 4002 4400 4674 4821 5232 2271
Size: 1731 398 274 147 411 2608
Fragments arranged by size:
2608 1731 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5344
Fragments arranged by size:
5344 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5569
BseYI C'CCAG_C
Cuts at: 4772 5268 4772
Size: 496 5073
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4996
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3019 3780 4808 1540
Size: 275 186 1018 761 1028 2301
Fragments arranged by size:
2301 1028 1018 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2595 3630 3715 4786 2595
Size: 1035 85 1071 3378
Fragments arranged by size:
3378 1071 1035 85
BsmI GAATG_Cn'
Cuts at: 3080 3173 3080
Size: 93 5476
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2342 2595 2888 3630 3715 4786 1391
Size: 399 552 253 293 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 552 399 293 253 85
BspEI T'CCGG_A
Cuts at: 5232 5232
Size: 5569
BspHI T'CATG_A
Cuts at: 3458 5188 3458
Size: 1730 3839
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2739 2924 844
Size: 1895 185 3489
Fragments arranged by size:
3489 1895 185
BsrBI CCG'CTC
Cuts at: 1394 1823 3456 1394
Size: 429 1633 3507
Fragments arranged by size:
3507 1633 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4064 4238 65
Size: 1183 2816 174 1396
Fragments arranged by size:
2816 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4213 4400 1700
Size: 110 2403 187 2869
Fragments arranged by size:
2869 2403 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5569
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5569
BssSI C'ACGA_G
Cuts at: 3629 4641 3629
Size: 1012 4557
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5569
BstBI TT'CG_AA
Cuts at: 4373 4373
Size: 5569
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5569
BstYI r'GATC_y
Cuts at: 1972 2578 2899 3266 3651 3668 5120 5206 5218
Size: 606 321 367 385 17 1452 86 12
Cuts at: 5218 5564 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 606 385 367 346 321 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4078
Fragments arranged by size:
4078 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4830
Fragments arranged by size:
4830 683 56
BtsI GCAGTG_nn'
Cuts at: 3085 3879 3899 3085
Size: 794 20 4755
Fragments arranged by size:
4755 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5569
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5534
DraI TTT'AAA
Cuts at: 2873 2873
Size: 5569
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2233 4576 809
Size: 507 917 2343 1802
Fragments arranged by size:
2343 1802 917 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3016 3905 5534
Size: 54 1425 50 275 18 1186 889 1629
Cuts at: 5534 8
Size: 43
Fragments arranged by size:
1629 1425 1186 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3016 1537
Size: 275 1204 4090
Fragments arranged by size:
4090 1204 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2169 3499 1353
Size: 816 1330 3423
Fragments arranged by size:
3423 1330 816
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4156 4530 4676 387
Size: 1064 138 2567 374 146 1280
Fragments arranged by size:
2567 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2593 2593
Size: 5569
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2381 2421 2520 3632 4229 5016 5284
Size: 393 749 40 99 1112 597 787 268
Cuts at: 5284 1239
Size: 1524
Fragments arranged by size:
1524 1112 787 749 597 393 268 99
40
EcoRI G'AATT_C
Cuts at: 2878 3256 2878
Size: 378 5191
EcoRV GAT'ATC
Cuts at: 2287 2287
Size: 5569
FspI TGC'GCA
Cuts at: 4075 4075
Size: 5569
HaeII r_GCGC'y
Cuts at: 1678 4716 1678
Size: 3038 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2302 2334 4210 4242 4284
Size: 32 135 32 730 32 1876 32 42
Cuts at: 4284 4316 1373
Size: 32 2626
Fragments arranged by size:
2626 1876 730 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2913 3159 669
Size: 988 281 975 246 3079
Fragments arranged by size:
3079 988 975 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5569
HpaI GTT'AAC
Cuts at: 3159 3159
Size: 5569
KpnI G_GTAC'C
Cuts at: 2894 2894
Size: 5569
MfeI C'AATT_G
Cuts at: 3168 3168
Size: 5569
MluI A'CGCG_T
Cuts at: 5445 5445
Size: 5569
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2486 2511 4683 4867 1305
Size: 1181 25 2172 184 2007
Fragments arranged by size:
2172 2007 1181 184 25
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3747
Fragments arranged by size:
3747 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2041 2347 3527 3886 4045 518
Size: 648 875 306 1180 359 159 2042
Fragments arranged by size:
2042 1180 875 648 359 306 159
MspA1I CmG'CkG
Cuts at: 1606 2985 3662 4810 5055 1606
Size: 1379 677 1148 245 2120
Fragments arranged by size:
2120 1379 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4078
Fragments arranged by size:
4078 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5569
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5569
NotI GC'GGCC_GC
Cuts at: 3016 3016
Size: 5569
NspI r_CATG'y
Cuts at: 1132 1229 2927 4472 1132
Size: 97 1698 1545 2229
Fragments arranged by size:
2229 1698 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5569
PciI A'CATG_T
Cuts at: 4468 4468
Size: 5569
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5569
PmeI GTTT'AAAC
Cuts at: 2873 2873
Size: 5569
PmlI CAC'GTG
Cuts at: 2116 2116
Size: 5569
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3638 3670 5151 5183 3638
Size: 32 1481 32 4024
Fragments arranged by size:
4024 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5569
PsiI TTA'TAA
Cuts at: 3139 3139
Size: 5569
PstI C_TGCA'G
Cuts at: 830 2921 830
Size: 2091 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5569
SacI G_AGCT'C
Cuts at: 2595 2595
Size: 5569
SalI G'TCGA_C
Cuts at: 1936 2911 1936
Size: 975 4594
SapI GCTCTTCn'nnn_
Cuts at: 2169 2169
Size: 5569
SbfI CC_TGCA'GG
Cuts at: 2921 2921
Size: 5569
ScaI AGT'ACT
Cuts at: 3817 5529 3817
Size: 1712 3857
SfcI C'TryA_G
Cuts at: 826 1045 2865 2917 4052 4733 4924 826
Size: 219 1820 52 1135 681 191 1471
Fragments arranged by size:
1820 1471 1135 681 219 191 52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5569
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5569
SmaI CCC'GGG
Cuts at: 1872 2896 1872
Size: 1024 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3673 4574 4836 820
Size: 197 926 1730 901 262 1553
Fragments arranged by size:
1730 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3360 493
Size: 2867 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5569
SphI G_CATG'C
Cuts at: 1132 2927 1132
Size: 1795 3774
SspI AAT'ATT
Cuts at: 5 52 2473 3493 5
Size: 47 2421 1020 2081
Fragments arranged by size:
2421 2081 1020 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5569
StyI C'CwwG_G
Cuts at: 513 1065 2004 2121 3008 513
Size: 552 939 117 887 3074
Fragments arranged by size:
3074 939 887 552 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3596 3613 3766 3951 4396 325
Size: 1255 2016 17 153 185 445 1498
Fragments arranged by size:
2016 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2262 3815 5527
Size: 274 80 33 51 1443 285 1553 1712
Cuts at: 5527 96
Size: 138
Fragments arranged by size:
1712 1553 1443 285 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2763 2795 5529 5561 2763
Size: 32 2734 32 2771
Fragments arranged by size:
2771 2734 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5569
XbaI T'CTAG_A
Cuts at: 2905 2905
Size: 5569
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2311 2311
Size: 5569
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5569
XmaI C'CCGG_G
Cuts at: 1870 2894 1870
Size: 1024 4545
XmnI GAAnn'nnTTC
Cuts at: 3698 3698
Size: 5569
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3377 276
Size: 53 83 186 936 1843 2468
Fragments arranged by size:
2468 1843 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PstI PvuI SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoNI EcoO109I FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI