Restriction map for
pFN21AE1777
(Circular) (Six-base) MAPSORT of: pFN21AE1777.seq Check: 6299 from: 1 to: 5560
pFN21AE1777 5560 bp
With 182 enzymes: SgfI *
March 28, 2012 12:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3370 278
Size: 53 83 186 936 1834 2468
Fragments arranged by size:
2468 1834 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2903 1937
Size: 966 4594
Acc65I G'GTAC_C
Cuts at: 2881 2881
Size: 5560
AclI AA'CG_TT
Cuts at: 1563 3687 4060 1563
Size: 2124 373 3063
Fragments arranged by size:
3063 2124 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2162 2559 2643 3623 5007 5275 1632
Size: 530 397 84 980 1384 268 1917
Fragments arranged by size:
1917 1384 980 530 397 268 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5363
AflIII A'CryG_T
Cuts at: 1203 4459 5436 1203
Size: 3256 977 1327
Fragments arranged by size:
3256 1327 977
AgeI A'CCGG_T
Cuts at: 4391 4391
Size: 5560
AhdI GACnn_n'nnGTC
Cuts at: 4289 4289
Size: 5560
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5560
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2394 1184
Size: 1210 4350
AlwNI CAG_nnn'CTG
Cuts at: 2666 3303 4875 2666
Size: 637 1572 3351
Fragments arranged by size:
3351 1572 637
ApaLI G'TGCA_C
Cuts at: 3617 4773 3617
Size: 1156 4404
ApoI r'AATT_y
Cuts at: 1493 2869 3095 3247 5356 1493
Size: 1376 226 152 2109 1697
Fragments arranged by size:
2109 1697 1376 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4114 160
Size: 872 3082 1606
Fragments arranged by size:
3082 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5560
AvaI C'yCGr_G
Cuts at: 1870 1943 2885 1870
Size: 73 942 4545
Fragments arranged by size:
4545 942 73
BamHI G'GATC_C
Cuts at: 2890 3257 2890
Size: 367 5193
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2126 2198 2881 4336 618
Size: 325 217 627 339 72 683 1455 1842
Fragments arranged by size:
1842 1455 683 627 339 325 217 72
BanII G_rGCy'C
Cuts at: 1391 2879 1391
Size: 1488 4072
BbeI G_GCGC'C
Cuts at: 2202 2202
Size: 5560
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2565 928
Size: 971 666 3923
Fragments arranged by size:
3923 971 666
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2116 2150 3751 3785 1903
Size: 34 50 34 95 34 1601 34 3678
Fragments arranged by size:
3678 1601 95 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3452 4662 3452
Size: 1210 4350
BclI T'GATC_A
Cuts at: 1548 2398 2506 1548
Size: 850 108 4602
Fragments arranged by size:
4602 850 108
BfrBI ATG'CAT
Cuts at: 2371 2375 2371
Size: 4 5556
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4171 136
Size: 107 122 71 631 605 2499 1525
Fragments arranged by size:
2499 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5555 5555
Size: 5560
BlpI GC'TnA_GC
Cuts at: 2173 2977 2173
Size: 804 4756
Bme1580I G_kGCm'C
Cuts at: 1790 2129 3621 4777 1790
Size: 339 1492 1156 2573
Fragments arranged by size:
2573 1492 1156 339
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5560
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3291 4249 442
Size: 416 53 83 399 24 1874 958 1753
Fragments arranged by size:
1874 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5560
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2119 2119
Size: 5560
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4220 1239
Size: 2981 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3685 4550 4848 3685
Size: 865 298 4397
Fragments arranged by size:
4397 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2107 4223 882
Size: 617 608 2116 2219
Fragments arranged by size:
2219 2116 617 608
BsaAI yAC'GTr
Cuts at: 493 1204 2471 2818 3351 493
Size: 711 1267 347 533 2702
Fragments arranged by size:
2702 1267 711 533 347
BsaBI GATnn'nnATC
Cuts at: 5554 5554
Size: 5560
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2199 3367 3749 4384
Size: 53 83 186 936 666 1168 382 635
Cuts at: 4384 275
Size: 1451
Fragments arranged by size:
1451 1168 936 666 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3993 4391 4665 4812 5223 3993
Size: 398 274 147 411 4330
Fragments arranged by size:
4330 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5335
Fragments arranged by size:
5335 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5560
BseYI C'CCAG_C
Cuts at: 2122 4763 5259 2122
Size: 2641 496 2423
Fragments arranged by size:
2641 2423 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4987
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3010 3771 4799 1540
Size: 275 186 1009 761 1028 2301
Fragments arranged by size:
2301 1028 1009 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2362 3621 3706 4777 2362
Size: 1259 85 1071 3145
Fragments arranged by size:
3145 1259 1071 85
BsmI GAATG_Cn'
Cuts at: 3071 3164 3071
Size: 93 5467
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2129 2362 2879 3621 3706 4777 1391
Size: 399 339 233 517 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 517 399 339 233 85
BspEI T'CCGG_A
Cuts at: 5223 5223
Size: 5560
BspHI T'CATG_A
Cuts at: 3449 5179 3449
Size: 1730 3830
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2166 2915 844
Size: 1322 749 3489
Fragments arranged by size:
3489 1322 749
BsrBI CCG'CTC
Cuts at: 1394 1823 2076 2289 3447 1394
Size: 429 253 213 1158 3507
Fragments arranged by size:
3507 1158 429 253 213
BsrDI GCAATG_nn'
Cuts at: 65 1248 4055 4229 65
Size: 1183 2807 174 1396
Fragments arranged by size:
2807 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4204 4391 1700
Size: 110 2394 187 2869
Fragments arranged by size:
2869 2394 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5560
BssHII G'CGCG_C
Cuts at: 1925 2015 1925
Size: 90 5470
BssSI C'ACGA_G
Cuts at: 3620 4632 3620
Size: 1012 4548
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2465 794
Size: 1671 3889
BstBI TT'CG_AA
Cuts at: 4364 4364
Size: 5560
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5560
BstXI CCAn_nnnn'nTGG
Cuts at: 2650 2650
Size: 5560
BstYI r'GATC_y
Cuts at: 1972 2435 2890 3257 3642 3659 5111 5197 5209
Size: 463 455 367 385 17 1452 86 12
Cuts at: 5209 5555 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 463 455 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5008
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2192 487
Size: 683 56 966 3855
Fragments arranged by size:
3855 966 683 56
BtsI GCAGTG_nn'
Cuts at: 2026 2849 3076 3870 3890 2026
Size: 823 227 794 20 3696
Fragments arranged by size:
3696 823 794 227 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5560
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5525
DraI TTT'AAA
Cuts at: 2864 2864
Size: 5560
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4567 809
Size: 507 3251 1802
Fragments arranged by size:
3251 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3007 3896 5525
Size: 54 1425 50 275 18 1177 889 1629
Cuts at: 5525 8
Size: 43
Fragments arranged by size:
1629 1425 1177 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3007 1537
Size: 275 1195 4090
Fragments arranged by size:
4090 1195 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3490 1353
Size: 2137 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2754 4147 4521 4667 387
Size: 1064 138 1165 1393 374 146 1280
Fragments arranged by size:
1393 1280 1165 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2162 2559 2643 3623 4220 5007 5275
Size: 393 530 397 84 980 597 787 268
Cuts at: 5275 1239
Size: 1524
Fragments arranged by size:
1524 980 787 597 530 397 393 268
84
EcoRI G'AATT_C
Cuts at: 2869 3247 2869
Size: 378 5182
FspI TGC'GCA
Cuts at: 4066 4066
Size: 5560
HaeII r_GCGC'y
Cuts at: 1678 2202 2684 4707 1678
Size: 524 482 2023 2531
Fragments arranged by size:
2531 2023 524 482
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2420 2452 4201 4233 4275
Size: 32 135 32 848 32 1749 32 42
Cuts at: 4275 4307 1373
Size: 32 2626
Fragments arranged by size:
2626 1749 848 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2904 3150 669
Size: 988 281 966 246 3079
Fragments arranged by size:
3079 988 966 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5560
HpaI GTT'AAC
Cuts at: 3150 3150
Size: 5560
KasI G'GCGC_C
Cuts at: 2198 2198
Size: 5560
KpnI G_GTAC'C
Cuts at: 2885 2885
Size: 5560
MfeI C'AATT_G
Cuts at: 3159 3159
Size: 5560
MluI A'CGCG_T
Cuts at: 5436 5436
Size: 5560
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2458 2585 2733 4674 4858 1305
Size: 1153 127 148 1941 184 2007
Fragments arranged by size:
2007 1941 1153 184 148 127
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3738
Fragments arranged by size:
3738 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2370 2376 2648 2789 3518 3877 4036
Size: 648 1204 6 272 141 729 359 159
Cuts at: 4036 518
Size: 2042
Fragments arranged by size:
2042 1204 729 648 359 272 159 141
6
MspA1I CmG'CkG
Cuts at: 1606 2031 2052 2064 2976 3653 4801 5046 1606
Size: 425 21 12 912 677 1148 245 2120
Fragments arranged by size:
2120 1148 912 677 425 245 21 12
NarI GG'CG_CC
Cuts at: 2199 2199
Size: 5560
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5008
NdeI CA'TA_TG
Cuts at: 387 2377 387
Size: 1990 3570
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5560
NotI GC'GGCC_GC
Cuts at: 3007 3007
Size: 5560
NsiI A_TGCA'T
Cuts at: 2373 2377 2373
Size: 4 5556
NspI r_CATG'y
Cuts at: 1132 1229 2371 2375 2463 2918 4463 1132
Size: 97 1142 4 88 455 1545 2229
Fragments arranged by size:
2229 1545 1142 455 97 88 4
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5560
PciI A'CATG_T
Cuts at: 4459 4459
Size: 5560
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2752 1108
Size: 1644 3916
PmeI GTTT'AAAC
Cuts at: 2864 2864
Size: 5560
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3629 3661 5142 5174 3629
Size: 32 1481 32 4015
Fragments arranged by size:
4015 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5560
PsiI TTA'TAA
Cuts at: 3130 3130
Size: 5560
PstI C_TGCA'G
Cuts at: 830 2662 2737 2912 830
Size: 1832 75 175 3478
Fragments arranged by size:
3478 1832 175 75
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5560
SalI G'TCGA_C
Cuts at: 1936 2902 1936
Size: 966 4594
SbfI CC_TGCA'GG
Cuts at: 2737 2912 2737
Size: 175 5385
ScaI AGT'ACT
Cuts at: 3808 5520 3808
Size: 1712 3848
SfcI C'TryA_G
Cuts at: 826 1045 2059 2280 2658 2733 2908 4043 4724
Size: 219 1014 221 378 75 175 1135 681
Cuts at: 4724 4915 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1014 681 378 221 219 191
175 75
SfoI GGC'GCC
Cuts at: 2200 2200
Size: 5560
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5560
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5560
SmaI CCC'GGG
Cuts at: 1872 2887 1872
Size: 1015 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3664 4565 4827 820
Size: 197 926 1721 901 262 1553
Fragments arranged by size:
1721 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3351 493
Size: 2858 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5560
SphI G_CATG'C
Cuts at: 1132 2375 2918 1132
Size: 1243 543 3774
Fragments arranged by size:
3774 1243 543
SspI AAT'ATT
Cuts at: 5 52 3484 5
Size: 47 3432 2081
Fragments arranged by size:
3432 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5560
StyI C'CwwG_G
Cuts at: 513 1065 2999 513
Size: 552 1934 3074
Fragments arranged by size:
3074 1934 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3587 3604 3757 3942 4387 325
Size: 1255 2007 17 153 185 445 1498
Fragments arranged by size:
2007 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2483 2584 3806
Size: 274 80 33 51 1443 506 101 1222
Cuts at: 3806 5518 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1222 506 274 138 101 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5520 5552 5520
Size: 32 5528
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5560
XbaI T'CTAG_A
Cuts at: 2896 2896
Size: 5560
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5560
XmaI C'CCGG_G
Cuts at: 1870 2885 1870
Size: 1015 4545
XmnI GAAnn'nnTTC
Cuts at: 3689 3689
Size: 5560
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3368 276
Size: 53 83 186 936 1834 2468
Fragments arranged by size:
2468 1834 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI XcmI