Restriction map for
pFN21AE1775
(Circular) (Six-base) MAPSORT of: pFN21AE1775.seq Check: 9540 from: 1 to: 5551
pFN21AE1775 5551 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3361 278
Size: 53 83 186 936 1825 2468
Fragments arranged by size:
2468 1825 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2894 1937
Size: 957 4594
Acc65I G'GTAC_C
Cuts at: 2667 2872 2667
Size: 205 5346
AclI AA'CG_TT
Cuts at: 1563 3678 4051 1563
Size: 2115 373 3063
Fragments arranged by size:
3063 2115 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2774 3614 4998 5266 1632
Size: 1142 840 1384 268 1917
Fragments arranged by size:
1917 1384 1142 840 268
AfeI AGC'GCT
Cuts at: 2090 2090
Size: 5551
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5354
AflIII A'CryG_T
Cuts at: 1203 4450 5427 1203
Size: 3247 977 1327
Fragments arranged by size:
3247 1327 977
AgeI A'CCGG_T
Cuts at: 2204 4382 2204
Size: 2178 3373
AhdI GACnn_n'nnGTC
Cuts at: 4280 4280
Size: 5551
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5551
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5551
AlwNI CAG_nnn'CTG
Cuts at: 2045 2274 2332 2497 2708 3294 4866 2045
Size: 229 58 165 211 586 1572 2730
Fragments arranged by size:
2730 1572 586 229 211 165 58
ApaI G_GGCC'C
Cuts at: 2596 2653 2596
Size: 57 5494
ApaLI G'TGCA_C
Cuts at: 2480 3608 4764 2480
Size: 1128 1156 3267
Fragments arranged by size:
3267 1156 1128
ApoI r'AATT_y
Cuts at: 1493 2860 3086 3238 5347 1493
Size: 1367 226 152 2109 1697
Fragments arranged by size:
2109 1697 1367 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4105 160
Size: 872 3073 1606
Fragments arranged by size:
3073 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5551
AvaI C'yCGr_G
Cuts at: 1870 1943 2056 2876 1870
Size: 73 113 820 4545
Fragments arranged by size:
4545 820 113 73
BamHI G'GATC_C
Cuts at: 2881 3248 2881
Size: 367 5184
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2631 2667 2698 2872 4327
Size: 325 217 627 844 36 31 174 1455
Cuts at: 4327 618
Size: 1842
Fragments arranged by size:
1842 1455 844 627 325 217 174 36
31
BanII G_rGCy'C
Cuts at: 1391 2513 2570 2596 2653 2836 2870 1391
Size: 1122 57 26 57 183 34 4072
Fragments arranged by size:
4072 1122 183 57 57 34 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2812 928
Size: 971 913 3667
Fragments arranged by size:
3667 971 913
BbvCI CC'TCA_GC
Cuts at: 2618 2618
Size: 5551
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2212 2246 3742 3776 1903
Size: 34 275 34 1496 34 3678
Fragments arranged by size:
3678 1496 275 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3443 4653 3443
Size: 1210 4341
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5551
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2629 4162 136
Size: 107 122 71 631 605 957 1533 1525
Fragments arranged by size:
1533 1525 957 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2572 5546 2572
Size: 2974 2577
BlpI GC'TnA_GC
Cuts at: 2968 2968
Size: 5551
Bme1580I G_kGCm'C
Cuts at: 1790 2484 2596 2634 2653 3612 4768 1790
Size: 694 112 38 19 959 1156 2573
Fragments arranged by size:
2573 1156 959 694 112 38 19
BmgBI CAC'GTC
Cuts at: 1318 2479 1318
Size: 1161 4390
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3282 4240 442
Size: 416 53 83 399 24 1865 958 1753
Fragments arranged by size:
1865 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5551
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2532 2532
Size: 5551
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2333 2392 2675 4211 1239
Size: 1094 59 283 1536 2579
Fragments arranged by size:
2579 1536 1094 283 59
Bpu10I CC'TnA_GC
Cuts at: 2618 2618
Size: 5551
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3676 4541 4839 3676
Size: 865 298 4388
Fragments arranged by size:
4388 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2545 4214 882
Size: 617 1046 1669 2219
Fragments arranged by size:
2219 1669 1046 617
BsaAI yAC'GTr
Cuts at: 493 1204 3342 493
Size: 711 2138 2702
Fragments arranged by size:
2702 2138 711
BsaBI GATnn'nnATC
Cuts at: 5545 5545
Size: 5551
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2160 2532 2817 3358
Size: 53 83 186 936 627 372 285 541
Cuts at: 3358 3740 4375 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 635 627 541 382 372 285
186 83 53
BsaWI w'CCGG_w
Cuts at: 2204 2216 3984 4382 4656 4803 5214 2204
Size: 12 1768 398 274 147 411 2541
Fragments arranged by size:
2541 1768 411 398 274 147 12
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2648 2651 2678 2681 1178
Size: 30 165 30 1245 3 27 3 4048
Fragments arranged by size:
4048 1245 165 30 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2366 2627 2630 2672 2726 2729 1186
Size: 1180 261 3 42 54 3 4008
Fragments arranged by size:
4008 1180 261 54 42 3 3
BseYI C'CCAG_C
Cuts at: 2332 2490 2504 2512 2675 4754 5250 2332
Size: 158 14 8 163 2079 496 2633
Fragments arranged by size:
2633 2079 496 163 158 14 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4978
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3001 3762 4790 1540
Size: 275 186 1000 761 1028 2301
Fragments arranged by size:
2301 1028 1000 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2484 3612 3697 4768 2484
Size: 1128 85 1071 3267
Fragments arranged by size:
3267 1128 1071 85
BsmI GAATG_Cn'
Cuts at: 2423 3062 3155 2423
Size: 639 93 4819
Fragments arranged by size:
4819 639 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2484 2513 2570 2596 2634 2653 2836
Size: 399 694 29 57 26 38 19 183
Cuts at: 2836 2870 3612 3697 4768 1391
Size: 34 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 694 399 183 85 57
38 34 29 26 19
BspEI T'CCGG_A
Cuts at: 2216 5214 2216
Size: 2998 2553
BspHI T'CATG_A
Cuts at: 3440 5170 3440
Size: 1730 3821
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2906 844
Size: 2062 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2010 3438 1394
Size: 429 187 1428 3507
Fragments arranged by size:
3507 1428 429 187
BsrDI GCAATG_nn'
Cuts at: 65 1248 4046 4220 65
Size: 1183 2798 174 1396
Fragments arranged by size:
2798 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2204 4195 4382 1700
Size: 110 394 1991 187 2869
Fragments arranged by size:
2869 1991 394 187 110
BsrGI T'GTAC_A
Cuts at: 96 2379 96
Size: 2283 3268
BssHII G'CGCG_C
Cuts at: 1925 2101 1925
Size: 176 5375
BssSI C'ACGA_G
Cuts at: 3611 4623 3611
Size: 1012 4539
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5551
BstBI TT'CG_AA
Cuts at: 4355 4355
Size: 5551
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5551
BstYI r'GATC_y
Cuts at: 1972 2572 2881 3248 3633 3650 5102 5188 5200
Size: 600 309 367 385 17 1452 86 12
Cuts at: 5200 5546 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 600 385 367 346 309 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2168 513
Size: 552 1103 3896
Fragments arranged by size:
3896 1103 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4812
Fragments arranged by size:
4812 683 56
BtsI GCAGTG_nn'
Cuts at: 3067 3861 3881 3067
Size: 794 20 4737
Fragments arranged by size:
4737 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5551
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5516
DraI TTT'AAA
Cuts at: 2855 2855
Size: 5551
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2537 4558 809
Size: 507 1221 2021 1802
Fragments arranged by size:
2021 1802 1221 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2998 3887 5516
Size: 54 1425 50 275 18 1168 889 1629
Cuts at: 5516 8
Size: 43
Fragments arranged by size:
1629 1425 1168 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2998 1537
Size: 275 1186 4090
Fragments arranged by size:
4090 1186 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3481 1353
Size: 2128 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4138 4512 4658 387
Size: 1064 138 2549 374 146 1280
Fragments arranged by size:
2549 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2333 2392 2675 2774 3614 4211 4998
Size: 393 701 59 283 99 840 597 787
Cuts at: 4998 5266 1239
Size: 268 1524
Fragments arranged by size:
1524 840 787 701 597 393 283 268
99 59
EcoNI CCTnn'n_nnAGG
Cuts at: 2402 2402
Size: 5551
EcoO109I rG'GnC_Cy
Cuts at: 2140 2246 2361 2464 2593 2649 2650 2728 2758
Size: 106 115 103 129 56 1 78 30
Cuts at: 2758 2140
Size: 4933
Fragments arranged by size:
4933 129 115 106 103 78 56 30
1
EcoRI G'AATT_C
Cuts at: 2860 3238 2860
Size: 378 5173
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2836 2836
Size: 5551
FspI TGC'GCA
Cuts at: 4057 4057
Size: 5551
HaeII r_GCGC'y
Cuts at: 1678 2092 2341 2440 4698 1678
Size: 414 249 99 2258 2531
Fragments arranged by size:
2531 2258 414 249 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2211 2243 2557 2589 4192
Size: 32 135 32 639 32 314 32 1603
Cuts at: 4192 4224 4266 4298 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1603 639 314 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2320 2895 3141 669
Size: 988 281 382 575 246 3079
Fragments arranged by size:
3079 988 575 382 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5551
HpaI GTT'AAC
Cuts at: 3141 3141
Size: 5551
KpnI G_GTAC'C
Cuts at: 2671 2876 2671
Size: 205 5346
MfeI C'AATT_G
Cuts at: 3150 3150
Size: 5551
MluI A'CGCG_T
Cuts at: 5427 5427
Size: 5551
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4665 4849 1305
Size: 3360 184 2007
Fragments arranged by size:
3360 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3729
Fragments arranged by size:
3729 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2297 2477 3509 3868 4027 518
Size: 648 1131 180 1032 359 159 2042
Fragments arranged by size:
2042 1131 1032 648 359 180 159
MspA1I CmG'CkG
Cuts at: 1606 2263 2443 2590 2967 3644 4792 5037 1606
Size: 657 180 147 377 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 657 377 245 180 147
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4999
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5551
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5551
NotI GC'GGCC_GC
Cuts at: 2998 2998
Size: 5551
NspI r_CATG'y
Cuts at: 1132 1229 2380 2393 2909 4454 1132
Size: 97 1151 13 516 1545 2229
Fragments arranged by size:
2229 1545 1151 516 97 13
PasI CC'CwG_GG
Cuts at: 1348 2497 1348
Size: 1149 4402
PciI A'CATG_T
Cuts at: 4450 4450
Size: 5551
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2332 2497 1108
Size: 1224 165 4162
Fragments arranged by size:
4162 1224 165
PfoI T'CCnGG_A
Cuts at: 2310 2754 2310
Size: 444 5107
PmeI GTTT'AAAC
Cuts at: 2855 2855
Size: 5551
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3620 3652 5133 5165 3620
Size: 32 1481 32 4006
Fragments arranged by size:
4006 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2140 2464 2758 2140
Size: 324 294 4933
Fragments arranged by size:
4933 324 294
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5551
PsiI TTA'TAA
Cuts at: 3121 3121
Size: 5551
PspOMI G'GGCC_C
Cuts at: 2592 2649 2592
Size: 57 5494
PstI C_TGCA'G
Cuts at: 830 2279 2349 2903 830
Size: 1449 70 554 3478
Fragments arranged by size:
3478 1449 554 70
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5551
PvuII CAG'CTG
Cuts at: 2263 2443 2263
Size: 180 5371
SalI G'TCGA_C
Cuts at: 1936 2893 1936
Size: 957 4594
SbfI CC_TGCA'GG
Cuts at: 2903 2903
Size: 5551
ScaI AGT'ACT
Cuts at: 3799 5511 3799
Size: 1712 3839
SfcI C'TryA_G
Cuts at: 826 1045 2275 2345 2899 4034 4715 4906 826
Size: 219 1230 70 554 1135 681 191 1471
Fragments arranged by size:
1471 1230 1135 681 554 219 191 70
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5551
SgrAI Cr'CCGG_yG
Cuts at: 1700 2204 1700
Size: 504 5047
SmaI CCC'GGG
Cuts at: 1872 2058 2878 1872
Size: 186 820 4545
Fragments arranged by size:
4545 820 186
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3655 4556 4818 820
Size: 197 926 1712 901 262 1553
Fragments arranged by size:
1712 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3342 493
Size: 2849 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5551
SphI G_CATG'C
Cuts at: 1132 2909 1132
Size: 1777 3774
SspI AAT'ATT
Cuts at: 5 52 3475 5
Size: 47 3423 2081
Fragments arranged by size:
3423 2081 47
StuI AGG'CCT
Cuts at: 1517 2623 1517
Size: 1106 4445
StyI C'CwwG_G
Cuts at: 513 1065 2990 513
Size: 552 1925 3074
Fragments arranged by size:
3074 1925 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2489 3578 3595 3748 3933 4378 325
Size: 1255 909 1089 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1089 909 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2379 3797 5509
Size: 274 80 33 51 1443 402 1418 1712
Cuts at: 5509 96
Size: 138
Fragments arranged by size:
1712 1443 1418 402 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2648 2680 5511 5543 2648
Size: 32 2831 32 2656
Fragments arranged by size:
2831 2656 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5551
XbaI T'CTAG_A
Cuts at: 2887 2887
Size: 5551
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2692 2692
Size: 5551
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5551
XmaI C'CCGG_G
Cuts at: 1870 2056 2876 1870
Size: 186 820 4545
Fragments arranged by size:
4545 820 186
XmnI GAAnn'nnTTC
Cuts at: 3680 3680
Size: 5551
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3359 276
Size: 53 83 186 936 1825 2468
Fragments arranged by size:
2468 1825 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI