Restriction map for
pFN21AE1766
(Circular) (Six-base) MAPSORT of: pFN21AE1766.seq Check: 24 from: 1 to: 5518
pFN21AE1766 5518 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2060 2060
Size: 5518
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3328 278
Size: 53 83 186 936 1792 2468
Fragments arranged by size:
2468 1792 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2861 1937
Size: 924 4594
Acc65I G'GTAC_C
Cuts at: 2839 2839
Size: 5518
AclI AA'CG_TT
Cuts at: 1563 3645 4018 1563
Size: 2082 373 3063
Fragments arranged by size:
3063 2082 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3581 4965 5233 1632
Size: 1949 1384 268 1917
Fragments arranged by size:
1949 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5321
AflIII A'CryG_T
Cuts at: 1203 2808 4417 5394 1203
Size: 1605 1609 977 1327
Fragments arranged by size:
1609 1605 1327 977
AgeI A'CCGG_T
Cuts at: 4349 4349
Size: 5518
AhdI GACnn_n'nnGTC
Cuts at: 4247 4247
Size: 5518
AleI CACnn'nnGTG
Cuts at: 1166 2355 1166
Size: 1189 4329
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5518
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2009 2041 2009
Size: 32 5486
AlwNI CAG_nnn'CTG
Cuts at: 2711 3261 4833 2711
Size: 550 1572 3396
Fragments arranged by size:
3396 1572 550
ApaI G_GGCC'C
Cuts at: 2573 2573
Size: 5518
ApaLI G'TGCA_C
Cuts at: 3575 4731 3575
Size: 1156 4362
ApoI r'AATT_y
Cuts at: 1493 2827 3053 3205 5314 1493
Size: 1334 226 152 2109 1697
Fragments arranged by size:
2109 1697 1334 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4072 160
Size: 872 3040 1606
Fragments arranged by size:
3040 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
AvaI C'yCGr_G
Cuts at: 1870 1943 2843 1870
Size: 73 900 4545
Fragments arranged by size:
4545 900 73
BamHI G'GATC_C
Cuts at: 2635 2848 3215 2635
Size: 213 367 4938
Fragments arranged by size:
4938 367 213
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2839 4294 618
Size: 325 217 627 1052 1455 1842
Fragments arranged by size:
1842 1455 1052 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2573 2728 2837 1391
Size: 1182 155 109 4072
Fragments arranged by size:
4072 1182 155 109
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2598 928
Size: 971 699 3848
Fragments arranged by size:
3848 971 699
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3709 3743 1903
Size: 34 1772 34 3678
Fragments arranged by size:
3678 1772 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3410 4620 3410
Size: 1210 4308
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5518
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4129 136
Size: 107 122 71 631 605 2457 1525
Fragments arranged by size:
2457 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2645 5513 2645
Size: 2868 2650
BlpI GC'TnA_GC
Cuts at: 2935 2935
Size: 5518
Bme1580I G_kGCm'C
Cuts at: 1790 2573 2718 3579 4735 1790
Size: 783 145 861 1156 2573
Fragments arranged by size:
2573 1156 861 783 145
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5518
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3249 4207 442
Size: 416 53 83 399 24 1832 958 1753
Fragments arranged by size:
1832 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2036 2688 4178 1239
Size: 797 652 1490 2579
Fragments arranged by size:
2579 1490 797 652
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3643 4508 4806 3643
Size: 865 298 4355
Fragments arranged by size:
4355 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2403 4181 882
Size: 617 904 1778 2219
Fragments arranged by size:
2219 1778 904 617
BsaAI yAC'GTr
Cuts at: 493 1204 3309 493
Size: 711 2105 2702
Fragments arranged by size:
2702 2105 711
BsaBI GATnn'nnATC
Cuts at: 5512 5512
Size: 5518
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3325 3707 4342 275
Size: 53 83 186 936 1792 382 635 1451
Fragments arranged by size:
1792 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2205 2511 3951 4349 4623 4770 5181 2205
Size: 306 1440 398 274 147 411 2542
Fragments arranged by size:
2542 1440 411 398 306 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2009 2039 2381 2411 1178
Size: 30 165 30 606 30 342 30 4285
Fragments arranged by size:
4285 606 342 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2402 1186
Size: 1216 4302
BseYI C'CCAG_C
Cuts at: 2171 4721 5217 2171
Size: 2550 496 2472
Fragments arranged by size:
2550 2472 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4945
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2968 3729 4757 1540
Size: 275 186 967 761 1028 2301
Fragments arranged by size:
2301 1028 967 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2665 2728 3579 3664 4735 2665
Size: 63 851 85 1071 3448
Fragments arranged by size:
3448 1071 851 85 63
BsmI GAATG_Cn'
Cuts at: 3029 3122 3029
Size: 93 5425
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2573 2665 2718 2728 2837 3579 3664
Size: 399 783 92 53 10 109 742 85
Cuts at: 3664 4735 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 783 742 399 109 92 85
53 10
BspEI T'CCGG_A
Cuts at: 2511 5181 2511
Size: 2670 2848
BspHI T'CATG_A
Cuts at: 3407 5137 3407
Size: 1730 3788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2060 2873 844
Size: 1216 813 3489
Fragments arranged by size:
3489 1216 813
BsrBI CCG'CTC
Cuts at: 1394 1823 3405 1394
Size: 429 1582 3507
Fragments arranged by size:
3507 1582 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4013 4187 65
Size: 1183 2765 174 1396
Fragments arranged by size:
2765 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2041 4162 4349 1700
Size: 110 231 2121 187 2869
Fragments arranged by size:
2869 2121 231 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5518
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5518
BssSI C'ACGA_G
Cuts at: 3578 4590 3578
Size: 1012 4506
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5518
BstBI TT'CG_AA
Cuts at: 4322 4322
Size: 5518
BstEII G'GTnAC_C
Cuts at: 1184 2126 1184
Size: 942 4576
BstXI CCAn_nnnn'nTGG
Cuts at: 2175 2257 2500 2175
Size: 82 243 5193
Fragments arranged by size:
5193 243 82
BstYI r'GATC_y
Cuts at: 1972 2635 2645 2848 3215 3600 3617 5069 5155
Size: 663 10 203 367 385 17 1452 86
Cuts at: 5155 5167 5513 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 663 385 367 346 203 86
17 12 10
Bsu36I CC'TnA_GG
Cuts at: 2680 2680
Size: 5518
BtgI C'CryG_G
Cuts at: 513 1065 2004 2256 513
Size: 552 939 252 3775
Fragments arranged by size:
3775 939 552 252
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4779
Fragments arranged by size:
4779 683 56
BtsI GCAGTG_nn'
Cuts at: 2444 3034 3828 3848 2444
Size: 590 794 20 4114
Fragments arranged by size:
4114 794 590 20
ClaI AT'CG_AT
Cuts at: 1554 2340 1554
Size: 786 4732
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5483
DraI TTT'AAA
Cuts at: 2822 2822
Size: 5518
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2069 2198 4525 809
Size: 507 753 129 2327 1802
Fragments arranged by size:
2327 1802 753 507 129
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2965 3854
Size: 54 1425 50 275 18 213 922 889
Cuts at: 3854 5483 8
Size: 1629 43
Fragments arranged by size:
1629 1425 922 889 275 213 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2965 1537
Size: 275 1153 4090
Fragments arranged by size:
4090 1153 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3448 1353
Size: 2095 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4105 4479 4625 387
Size: 1064 138 2516 374 146 1280
Fragments arranged by size:
2516 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2726 2726
Size: 5518
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2688 3581 4178 4965 5233 1239
Size: 393 404 652 893 597 787 268 1524
Fragments arranged by size:
1524 893 787 652 597 404 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2616 2679 2616
Size: 63 5455
EcoO109I rG'GnC_Cy
Cuts at: 2138 2370 2138
Size: 232 5286
EcoRI G'AATT_C
Cuts at: 2827 3205 2827
Size: 378 5140
FspI TGC'GCA
Cuts at: 4024 4024
Size: 5518
HaeII r_GCGC'y
Cuts at: 1678 2042 2712 4665 1678
Size: 364 670 1953 2531
Fragments arranged by size:
2531 1953 670 364
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2138 2170 2381 2413 2620
Size: 32 135 32 566 32 211 32 207
Cuts at: 2620 2652 2663 2695 4159 4191 4233 4265 1373
Size: 32 11 32 1464 32 42 32 2626
Fragments arranged by size:
2626 1464 566 211 207 135 42 32
32 32 32 32 32 32 32 11
HincII GTy'rAC
Cuts at: 669 1657 1938 2862 3108 669
Size: 988 281 924 246 3079
Fragments arranged by size:
3079 988 924 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5518
HpaI GTT'AAC
Cuts at: 3108 3108
Size: 5518
KpnI G_GTAC'C
Cuts at: 2843 2843
Size: 5518
MfeI C'AATT_G
Cuts at: 3117 3117
Size: 5518
MluI A'CGCG_T
Cuts at: 5394 5394
Size: 5518
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4632 4816 1305
Size: 3327 184 2007
Fragments arranged by size:
3327 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3696
Fragments arranged by size:
3696 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2255 2355 2630 3476 3835 3994 518
Size: 648 1089 100 275 846 359 159 2042
Fragments arranged by size:
2042 1089 846 648 359 275 159 100
MspA1I CmG'CkG
Cuts at: 1606 2171 2934 3611 4759 5004 1606
Size: 565 763 677 1148 245 2120
Fragments arranged by size:
2120 1148 763 677 565 245
NaeI GCC'GGC
Cuts at: 2043 2043
Size: 5518
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4027
Fragments arranged by size:
4027 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5518
NgoMIV G'CCGG_C
Cuts at: 2041 2041
Size: 5518
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5518
NotI GC'GGCC_GC
Cuts at: 2965 2965
Size: 5518
NspI r_CATG'y
Cuts at: 1132 1229 2812 2876 4421 1132
Size: 97 1583 64 1545 2229
Fragments arranged by size:
2229 1583 1545 97 64
PasI CC'CwG_GG
Cuts at: 1348 2566 1348
Size: 1218 4300
PciI A'CATG_T
Cuts at: 2808 4417 2808
Size: 1609 3909
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5518
PmeI GTTT'AAAC
Cuts at: 2822 2822
Size: 5518
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2009 2041 3587 3619 5100 5132 2009
Size: 32 1546 32 1481 32 2395
Fragments arranged by size:
2395 1546 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2138 2370 2138
Size: 232 5286
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5518
PsiI TTA'TAA
Cuts at: 3088 3088
Size: 5518
PspOMI G'GGCC_C
Cuts at: 2569 2569
Size: 5518
PstI C_TGCA'G
Cuts at: 830 2057 2870 830
Size: 1227 813 3478
Fragments arranged by size:
3478 1227 813
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5518
PvuII CAG'CTG
Cuts at: 2171 2171
Size: 5518
SacI G_AGCT'C
Cuts at: 2728 2728
Size: 5518
SalI G'TCGA_C
Cuts at: 1936 2860 1936
Size: 924 4594
SanDI GG'GwC_CC
Cuts at: 2138 2138
Size: 5518
SbfI CC_TGCA'GG
Cuts at: 2057 2870 2057
Size: 813 4705
ScaI AGT'ACT
Cuts at: 3766 5478 3766
Size: 1712 3806
SexAI A'CCwGG_T
Cuts at: 2814 2814
Size: 5518
SfcI C'TryA_G
Cuts at: 826 1045 2053 2866 4001 4682 4873 826
Size: 219 1008 813 1135 681 191 1471
Fragments arranged by size:
1471 1135 1008 813 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5518
SmaI CCC'GGG
Cuts at: 1872 2845 1872
Size: 973 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3622 4523 4785 820
Size: 197 926 1679 901 262 1553
Fragments arranged by size:
1679 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3309 493
Size: 2816 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5518
SphI G_CATG'C
Cuts at: 1132 2876 1132
Size: 1744 3774
SspI AAT'ATT
Cuts at: 5 52 3442 5
Size: 47 3390 2081
Fragments arranged by size:
3390 2081 47
StuI AGG'CCT
Cuts at: 1517 2685 1517
Size: 1168 4350
StyI C'CwwG_G
Cuts at: 513 1065 2004 2957 513
Size: 552 939 953 3074
Fragments arranged by size:
3074 953 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2108 2163 2345 2385 2544 3545 3562
Size: 1255 528 55 182 40 159 1001 17
Cuts at: 3562 3715 3900 4345 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1001 528 445 185 182 159
153 55 40 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3764 5476 96
Size: 274 80 33 51 1443 1787 1712 138
Fragments arranged by size:
1787 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5478 5510 5478
Size: 32 5486
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5518
XbaI T'CTAG_A
Cuts at: 2854 2854
Size: 5518
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5518
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5518
XmaI C'CCGG_G
Cuts at: 1870 2843 1870
Size: 973 4545
XmnI GAAnn'nnTTC
Cuts at: 3647 3647
Size: 5518
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3326 276
Size: 53 83 186 936 1792 2468
Fragments arranged by size:
2468 1792 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI
SwaI