Restriction map for
pFN21AE1740
(Circular) (Six-base) MAPSORT of: pFN21AE1740.seq Check: 9749 from: 1 to: 5437
pFN21AE1740 5437 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3247 278
Size: 53 83 186 936 1711 2468
Fragments arranged by size:
2468 1711 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2334 2780 1937
Size: 397 446 4594
Fragments arranged by size:
4594 446 397
Acc65I G'GTAC_C
Cuts at: 2758 2758
Size: 5437
AclI AA'CG_TT
Cuts at: 1563 2736 3564 3937 1563
Size: 1173 828 373 3063
Fragments arranged by size:
3063 1173 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2289 3500 4884 5152 1632
Size: 657 1211 1384 268 1917
Fragments arranged by size:
1917 1384 1211 657 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5240
AflIII A'CryG_T
Cuts at: 1203 4336 5313 1203
Size: 3133 977 1327
Fragments arranged by size:
3133 1327 977
AgeI A'CCGG_T
Cuts at: 4268 4268
Size: 5437
AhdI GACnn_n'nnGTC
Cuts at: 4166 4166
Size: 5437
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5437
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5437
AlwNI CAG_nnn'CTG
Cuts at: 3180 4752 3180
Size: 1572 3865
ApaI G_GGCC'C
Cuts at: 2079 2161 2079
Size: 82 5355
ApaLI G'TGCA_C
Cuts at: 3494 4650 3494
Size: 1156 4281
ApoI r'AATT_y
Cuts at: 1493 2450 2746 2972 3124 5233 1493
Size: 957 296 226 152 2109 1697
Fragments arranged by size:
2109 1697 957 296 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3991 160
Size: 872 2959 1606
Fragments arranged by size:
2959 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5437
AvaI C'yCGr_G
Cuts at: 1870 1943 2261 2277 2762 1870
Size: 73 318 16 485 4545
Fragments arranged by size:
4545 485 318 73 16
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2241 2274 2241
Size: 33 5404
BamHI G'GATC_C
Cuts at: 2767 3134 2767
Size: 367 5070
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2183 2324 2758 4213 618
Size: 325 217 627 396 141 434 1455 1842
Fragments arranged by size:
1842 1455 627 434 396 325 217 141
BanII G_rGCy'C
Cuts at: 1391 2079 2161 2756 1391
Size: 688 82 595 4072
Fragments arranged by size:
4072 688 595 82
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4466
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3628 3662 1903
Size: 34 50 34 1607 34 3678
Fragments arranged by size:
3678 1607 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3329 4539 3329
Size: 1210 4227
BclI T'GATC_A
Cuts at: 1548 2563 1548
Size: 1015 4422
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2172 4048 136
Size: 107 122 71 631 605 500 1876 1525
Fragments arranged by size:
1876 1525 631 605 500 122 107 71
BglII A'GATC_T
Cuts at: 2406 5432 2406
Size: 3026 2411
BlpI GC'TnA_GC
Cuts at: 2341 2854 2341
Size: 513 4924
Bme1580I G_kGCm'C
Cuts at: 1790 2079 2161 3498 4654 1790
Size: 289 82 1337 1156 2573
Fragments arranged by size:
2573 1337 1156 289 82
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5437
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3168 4126 442
Size: 416 53 83 399 24 1751 958 1753
Fragments arranged by size:
1753 1751 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5437
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2337 2627 2639 4097 1239
Size: 1098 290 12 1458 2579
Fragments arranged by size:
2579 1458 1098 290 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2055 3562 4427 4725 2055
Size: 1507 865 298 2767
Fragments arranged by size:
2767 1507 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4100 882
Size: 617 2601 2219
Fragments arranged by size:
2601 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3228 493
Size: 711 2024 2702
Fragments arranged by size:
2702 2024 711
BsaBI GATnn'nnATC
Cuts at: 5431 5431
Size: 5437
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3244 3626 4261 275
Size: 53 83 186 936 1711 382 635 1451
Fragments arranged by size:
1711 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2252 3870 4268 4542 4689 5100 2252
Size: 1618 398 274 147 411 2589
Fragments arranged by size:
2589 1618 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2579 2609 2681 2711 1178
Size: 30 165 30 1176 30 72 30 3904
Fragments arranged by size:
3904 1176 165 72 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5437
BseYI C'CCAG_C
Cuts at: 2014 4640 5136 2014
Size: 2626 496 2315
Fragments arranged by size:
2626 2315 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2095 1163
Size: 573 359 4505
Fragments arranged by size:
4505 573 359
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2887 3648 4676 1540
Size: 275 186 886 761 1028 2301
Fragments arranged by size:
2301 1028 886 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2610 3498 3583 4654 2610
Size: 888 85 1071 3393
Fragments arranged by size:
3393 1071 888 85
BsmI GAATG_Cn'
Cuts at: 2345 2948 3041 2345
Size: 603 93 4741
Fragments arranged by size:
4741 603 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2161 2610 2756 3498 3583 4654
Size: 399 289 82 449 146 742 85 1071
Cuts at: 4654 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 449 399 289 146 85
82
BspEI T'CCGG_A
Cuts at: 5100 5100
Size: 5437
BspHI T'CATG_A
Cuts at: 2050 3326 5056 2050
Size: 1276 1730 2431
Fragments arranged by size:
2431 1730 1276
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2792 844
Size: 1948 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2340 3324 1394
Size: 429 517 984 3507
Fragments arranged by size:
3507 984 517 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3932 4106 65
Size: 1183 2684 174 1396
Fragments arranged by size:
2684 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4081 4268 1700
Size: 110 2271 187 2869
Fragments arranged by size:
2869 2271 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5437
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5437
BssSI C'ACGA_G
Cuts at: 3497 4509 3497
Size: 1012 4425
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5437
BstBI TT'CG_AA
Cuts at: 4241 4241
Size: 5437
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5437
BstXI CCAn_nnnn'nTGG
Cuts at: 2435 2651 2435
Size: 216 5221
BstYI r'GATC_y
Cuts at: 1972 2212 2406 2767 3134 3519 3536 4988 5074
Size: 240 194 361 367 385 17 1452 86
Cuts at: 5074 5086 5432 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 361 346 240 194
86 17 12
BstZ17I GTA'TAC
Cuts at: 2335 2335
Size: 5437
Bsu36I CC'TnA_GG
Cuts at: 2232 2232
Size: 5437
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4885
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4698
Fragments arranged by size:
4698 683 56
BtsI GCAGTG_nn'
Cuts at: 2455 2953 3747 3767 2455
Size: 498 794 20 4125
Fragments arranged by size:
4125 794 498 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5437
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5402
DraI TTT'AAA
Cuts at: 2741 2741
Size: 5437
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4444 809
Size: 507 3128 1802
Fragments arranged by size:
3128 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2884 3773 5402
Size: 54 1425 50 275 18 1054 889 1629
Cuts at: 5402 8
Size: 43
Fragments arranged by size:
1629 1425 1054 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2884 1537
Size: 275 1072 4090
Fragments arranged by size:
4090 1072 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2534 2558 3367 1353
Size: 1181 24 809 3423
Fragments arranged by size:
3423 1181 809 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4024 4398 4544 387
Size: 1064 138 2435 374 146 1280
Fragments arranged by size:
2435 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2289 2337 2627 2639 3500 4097 4884
Size: 393 657 48 290 12 861 597 787
Cuts at: 4884 5152 1239
Size: 268 1524
Fragments arranged by size:
1524 861 787 657 597 393 290 268
48 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2099 2319 2099
Size: 220 5217
EcoO109I rG'GnC_Cy
Cuts at: 2075 2106 2157 2158 2273 2075
Size: 31 51 1 115 5239
Fragments arranged by size:
5239 115 51 31 1
EcoRI G'AATT_C
Cuts at: 2746 3124 2746
Size: 378 5059
FspI TGC'GCA
Cuts at: 3943 3943
Size: 5437
HaeII r_GCGC'y
Cuts at: 1678 4584 1678
Size: 2906 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2579 2611 2681 2713 4078
Size: 32 135 32 1007 32 70 32 1365
Cuts at: 4078 4110 4152 4184 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1365 1007 135 70 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2781 3027 669
Size: 988 281 843 246 3079
Fragments arranged by size:
3079 988 843 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5437
HpaI GTT'AAC
Cuts at: 3027 3027
Size: 5437
KpnI G_GTAC'C
Cuts at: 2762 2762
Size: 5437
MfeI C'AATT_G
Cuts at: 3036 3036
Size: 5437
MluI A'CGCG_T
Cuts at: 5313 5313
Size: 5437
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2099 4551 4735 1305
Size: 794 2452 184 2007
Fragments arranged by size:
2452 2007 794 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3615
Fragments arranged by size:
3615 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2433 3395 3754 3913 518
Size: 648 1267 962 359 159 2042
Fragments arranged by size:
2042 1267 962 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2853 3530 4678 4923 1606
Size: 1247 677 1148 245 2120
Fragments arranged by size:
2120 1247 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4885
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5437
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5437
NotI GC'GGCC_GC
Cuts at: 2884 2884
Size: 5437
NspI r_CATG'y
Cuts at: 1132 1229 2389 2795 4340 1132
Size: 97 1160 406 1545 2229
Fragments arranged by size:
2229 1545 1160 406 97
PasI CC'CwG_GG
Cuts at: 1348 2135 1348
Size: 787 4650
PciI A'CATG_T
Cuts at: 4336 4336
Size: 5437
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5437
PfoI T'CCnGG_A
Cuts at: 2287 2287
Size: 5437
PmeI GTTT'AAAC
Cuts at: 2741 2741
Size: 5437
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3506 3538 5019 5051 3506
Size: 32 1481 32 3892
Fragments arranged by size:
3892 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2273 2273
Size: 5437
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5437
PsiI TTA'TAA
Cuts at: 3007 3007
Size: 5437
PspOMI G'GGCC_C
Cuts at: 2075 2157 2075
Size: 82 5355
PstI C_TGCA'G
Cuts at: 830 2789 830
Size: 1959 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5437
SalI G'TCGA_C
Cuts at: 1936 2779 1936
Size: 843 4594
SapI GCTCTTCn'nnn_
Cuts at: 2558 2558
Size: 5437
SbfI CC_TGCA'GG
Cuts at: 2789 2789
Size: 5437
ScaI AGT'ACT
Cuts at: 3685 5397 3685
Size: 1712 3725
SfcI C'TryA_G
Cuts at: 826 1045 2785 3920 4601 4792 826
Size: 219 1740 1135 681 191 1471
Fragments arranged by size:
1740 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5437
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5437
SmaI CCC'GGG
Cuts at: 1872 2263 2764 1872
Size: 391 501 4545
Fragments arranged by size:
4545 501 391
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2070 3541 4442 4704 820
Size: 197 926 127 1471 901 262 1553
Fragments arranged by size:
1553 1471 926 901 262 197 127
SnaBI TAC'GTA
Cuts at: 493 3228 493
Size: 2735 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5437
SphI G_CATG'C
Cuts at: 1132 2795 1132
Size: 1663 3774
SspI AAT'ATT
Cuts at: 5 52 3361 5
Size: 47 3309 2081
Fragments arranged by size:
3309 2081 47
StuI AGG'CCT
Cuts at: 1517 2594 1517
Size: 1077 4360
StyI C'CwwG_G
Cuts at: 513 1065 2100 2296 2876 513
Size: 552 1035 196 580 3074
Fragments arranged by size:
3074 1035 580 552 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3464 3481 3634 3819 4264 325
Size: 1255 1884 17 153 185 445 1498
Fragments arranged by size:
1884 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2475 3683 5395
Size: 274 80 33 51 1443 498 1208 1712
Cuts at: 5395 96
Size: 138
Fragments arranged by size:
1712 1443 1208 498 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5397 5429 5397
Size: 32 5405
Tth111I GACn'n_nGTC
Cuts at: 1316 2633 1316
Size: 1317 4120
XbaI T'CTAG_A
Cuts at: 2773 2773
Size: 5437
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5437
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5437
XmaI C'CCGG_G
Cuts at: 1870 2261 2762 1870
Size: 391 501 4545
Fragments arranged by size:
4545 501 391
XmnI GAAnn'nnTTC
Cuts at: 3566 3566
Size: 5437
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3245 276
Size: 53 83 186 936 1711 2468
Fragments arranged by size:
2468 1711 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI PvuII RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI