Restriction map for
pFN21AE1728
(Circular) (Six-base) MAPSORT of: pFN21AE1728.seq Check: 4387 from: 1 to: 5413
pFN21AE1728 5413 bp
With 182 enzymes: SgfI *
July 6, 2012 17:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3223 278
Size: 53 83 186 936 1687 2468
Fragments arranged by size:
2468 1687 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2756 1937
Size: 819 4594
Acc65I G'GTAC_C
Cuts at: 2734 2734
Size: 5413
AclI AA'CG_TT
Cuts at: 1563 3540 3913 1563
Size: 1977 373 3063
Fragments arranged by size:
3063 1977 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3476 4860 5128 1632
Size: 1844 1384 268 1917
Fragments arranged by size:
1917 1844 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5216
AflIII A'CryG_T
Cuts at: 1203 4312 5289 1203
Size: 3109 977 1327
Fragments arranged by size:
3109 1327 977
AgeI A'CCGG_T
Cuts at: 4244 4244
Size: 5413
AhdI GACnn_n'nnGTC
Cuts at: 2414 4142 2414
Size: 1728 3685
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5413
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2125 1184
Size: 941 4472
AlwNI CAG_nnn'CTG
Cuts at: 2060 3156 4728 2060
Size: 1096 1572 2745
Fragments arranged by size:
2745 1572 1096
ApaLI G'TGCA_C
Cuts at: 3470 4626 3470
Size: 1156 4257
ApoI r'AATT_y
Cuts at: 1493 2722 2948 3100 5209 1493
Size: 1229 226 152 2109 1697
Fragments arranged by size:
2109 1697 1229 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3967 160
Size: 872 2935 1606
Fragments arranged by size:
2935 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5413
AvaI C'yCGr_G
Cuts at: 1870 1943 2023 2738 1870
Size: 73 80 715 4545
Fragments arranged by size:
4545 715 80 73
AvrII C'CTAG_G
Cuts at: 2467 2467
Size: 5413
BamHI G'GATC_C
Cuts at: 2149 2255 2484 2743 3110 2149
Size: 106 229 259 367 4452
Fragments arranged by size:
4452 367 259 229 106
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2734 4189 618
Size: 325 217 627 947 1455 1842
Fragments arranged by size:
1842 1455 947 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2661 2732 1391
Size: 1270 71 4072
Fragments arranged by size:
4072 1270 71
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4442
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2233 2267 3604 3638 1903
Size: 34 296 34 1337 34 3678
Fragments arranged by size:
3678 1337 296 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3305 4515 3305
Size: 1210 4203
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5413
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2598 4024 136
Size: 107 122 71 631 605 926 1426 1525
Fragments arranged by size:
1525 1426 926 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2012 2673 5408 2012
Size: 661 2735 2017
Fragments arranged by size:
2735 2017 661
BlpI GC'TnA_GC
Cuts at: 2240 2342 2830 2240
Size: 102 488 4823
Fragments arranged by size:
4823 488 102
Bme1580I G_kGCm'C
Cuts at: 1790 3474 4630 1790
Size: 1684 1156 2573
Fragments arranged by size:
2573 1684 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5413
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2420 2456 2554
Size: 416 53 83 399 24 1003 36 98
Cuts at: 2554 3144 4102 442
Size: 590 958 1753
Fragments arranged by size:
1753 1003 958 590 416 399 98 83
53 36 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5413
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2416 4073 1239
Size: 1177 1657 2579
Fragments arranged by size:
2579 1657 1177
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2474 3538 4403 4701 2474
Size: 1064 865 298 3186
Fragments arranged by size:
3186 1064 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4076 882
Size: 617 2577 2219
Fragments arranged by size:
2577 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2137 3204 493
Size: 711 933 1067 2702
Fragments arranged by size:
2702 1067 933 711
BsaBI GATnn'nnATC
Cuts at: 5407 5407
Size: 5413
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2415 3220 3602 4237
Size: 53 83 186 936 882 805 382 635
Cuts at: 4237 275
Size: 1451
Fragments arranged by size:
1451 936 882 805 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3846 4244 4518 4665 5076 3846
Size: 398 274 147 411 4183
Fragments arranged by size:
4183 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2019 2049 1178
Size: 30 165 30 616 30 4542
Fragments arranged by size:
4542 616 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2722 1186
Size: 1536 3877
BseYI C'CCAG_C
Cuts at: 2128 2153 2571 4616 5112 2128
Size: 25 418 2045 496 2429
Fragments arranged by size:
2429 2045 496 418 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4840
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2863 3624 4652 1540
Size: 275 186 862 761 1028 2301
Fragments arranged by size:
2301 1028 862 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2424 2430 3474 3559 4630 2424
Size: 6 1044 85 1071 3207
Fragments arranged by size:
3207 1071 1044 85 6
BsmI GAATG_Cn'
Cuts at: 2924 3017 2924
Size: 93 5320
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2424 2430 2661 2732 3474 3559 4630
Size: 399 634 6 231 71 742 85 1071
Cuts at: 4630 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 634 399 231 85 71
6
BspEI T'CCGG_A
Cuts at: 5076 5076
Size: 5413
BspHI T'CATG_A
Cuts at: 3302 5032 3302
Size: 1730 3683
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2768 844
Size: 1924 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2194 3300 1394
Size: 429 371 1106 3507
Fragments arranged by size:
3507 1106 429 371
BsrDI GCAATG_nn'
Cuts at: 65 1248 3908 4082 65
Size: 1183 2660 174 1396
Fragments arranged by size:
2660 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2679 4057 4244 1700
Size: 110 869 1378 187 2869
Fragments arranged by size:
2869 1378 869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5413
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5413
BssSI C'ACGA_G
Cuts at: 2423 3473 4485 2423
Size: 1050 1012 3351
Fragments arranged by size:
3351 1050 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2338 794
Size: 1544 3869
BstBI TT'CG_AA
Cuts at: 4217 4217
Size: 5413
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5413
BstXI CCAn_nnnn'nTGG
Cuts at: 2161 2566 2161
Size: 405 5008
BstYI r'GATC_y
Cuts at: 1972 2012 2149 2255 2484 2673 2743 3110 3495
Size: 40 137 106 229 189 70 367 385
Cuts at: 3495 3512 4964 5050 5062 5408 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 229 189 137
106 86 70 40 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2376 2595 513
Size: 552 1311 219 3331
Fragments arranged by size:
3331 1311 552 219
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4674
Fragments arranged by size:
4674 683 56
BtsI GCAGTG_nn'
Cuts at: 2320 2929 3723 3743 2320
Size: 609 794 20 3990
Fragments arranged by size:
3990 794 609 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5413
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5378
DraI TTT'AAA
Cuts at: 2717 2717
Size: 5413
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4420 809
Size: 507 3104 1802
Fragments arranged by size:
3104 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2860 3749 5378
Size: 54 1425 50 275 18 1030 889 1629
Cuts at: 5378 8
Size: 43
Fragments arranged by size:
1629 1425 1030 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2860 1537
Size: 275 1048 4090
Fragments arranged by size:
4090 1048 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2003 2649 3343 1353
Size: 650 646 694 3423
Fragments arranged by size:
3423 694 650 646
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4000 4374 4520 387
Size: 1064 138 2411 374 146 1280
Fragments arranged by size:
2411 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2416 3476 4073 4860 5128 1239
Size: 393 784 1060 597 787 268 1524
Fragments arranged by size:
1524 1060 787 784 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2492 2492
Size: 5413
EcoO109I rG'GnC_Cy
Cuts at: 2057 2247 2697 2057
Size: 190 450 4773
Fragments arranged by size:
4773 450 190
EcoRI G'AATT_C
Cuts at: 2722 3100 2722
Size: 378 5035
FspI TGC'GCA
Cuts at: 3919 3919
Size: 5413
HaeII r_GCGC'y
Cuts at: 1678 4560 1678
Size: 2882 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2400 2432 4054 4086 4128
Size: 32 135 32 828 32 1622 32 42
Cuts at: 4128 4160 1373
Size: 32 2626
Fragments arranged by size:
2626 1622 828 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2757 3003 669
Size: 988 281 819 246 3079
Fragments arranged by size:
3079 988 819 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5413
HpaI GTT'AAC
Cuts at: 3003 3003
Size: 5413
KpnI G_GTAC'C
Cuts at: 2738 2738
Size: 5413
MfeI C'AATT_G
Cuts at: 3012 3012
Size: 5413
MluI A'CGCG_T
Cuts at: 5289 5289
Size: 5413
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4527 4711 1305
Size: 3222 184 2007
Fragments arranged by size:
3222 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3591
Fragments arranged by size:
3591 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3371 3730 3889 518
Size: 648 2205 359 159 2042
Fragments arranged by size:
2205 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2378 2669 2829 3506 4654 4899 1606
Size: 772 291 160 677 1148 245 2120
Fragments arranged by size:
2120 1148 772 677 291 245 160
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4861
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5413
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5413
NotI GC'GGCC_GC
Cuts at: 2860 2860
Size: 5413
NspI r_CATG'y
Cuts at: 1132 1229 2771 4316 1132
Size: 97 1542 1545 2229
Fragments arranged by size:
2229 1545 1542 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5413
PciI A'CATG_T
Cuts at: 4312 4312
Size: 5413
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2544 1108
Size: 1436 3977
PfoI T'CCnGG_A
Cuts at: 2526 2526
Size: 5413
PmeI GTTT'AAAC
Cuts at: 2717 2717
Size: 5413
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3482 3514 4995 5027 3482
Size: 32 1481 32 3868
Fragments arranged by size:
3868 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2697 2697
Size: 5413
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5413
PsiI TTA'TAA
Cuts at: 2983 2983
Size: 5413
PstI C_TGCA'G
Cuts at: 830 2765 830
Size: 1935 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5413
SacII CC_GC'GG
Cuts at: 2379 2379
Size: 5413
SalI G'TCGA_C
Cuts at: 1936 2755 1936
Size: 819 4594
SanDI GG'GwC_CC
Cuts at: 2697 2697
Size: 5413
SapI GCTCTTCn'nnn_
Cuts at: 2649 2649
Size: 5413
SbfI CC_TGCA'GG
Cuts at: 2765 2765
Size: 5413
ScaI AGT'ACT
Cuts at: 3661 5373 3661
Size: 1712 3701
SfcI C'TryA_G
Cuts at: 826 1045 2383 2761 3896 4577 4768 826
Size: 219 1338 378 1135 681 191 1471
Fragments arranged by size:
1471 1338 1135 681 378 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5413
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5413
SmaI CCC'GGG
Cuts at: 1872 2025 2740 1872
Size: 153 715 4545
Fragments arranged by size:
4545 715 153
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2489 3517 4418 4680 820
Size: 197 926 546 1028 901 262 1553
Fragments arranged by size:
1553 1028 926 901 546 262 197
SnaBI TAC'GTA
Cuts at: 493 3204 493
Size: 2711 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5413
SphI G_CATG'C
Cuts at: 1132 2771 1132
Size: 1639 3774
SspI AAT'ATT
Cuts at: 5 52 2086 3337 5
Size: 47 2034 1251 2081
Fragments arranged by size:
2081 2034 1251 47
StuI AGG'CCT
Cuts at: 1517 2076 1517
Size: 559 4854
StyI C'CwwG_G
Cuts at: 513 1065 2467 2852 513
Size: 552 1402 385 3074
Fragments arranged by size:
3074 1402 552 385
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3440 3457 3610 3795 4240 325
Size: 1255 1860 17 153 185 445 1498
Fragments arranged by size:
1860 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2541 3659 5371
Size: 274 80 33 51 1443 564 1118 1712
Cuts at: 5371 96
Size: 138
Fragments arranged by size:
1712 1443 1118 564 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2173 2205 5373 5405 2173
Size: 32 3168 32 2181
Fragments arranged by size:
3168 2181 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5413
XbaI T'CTAG_A
Cuts at: 2749 2749
Size: 5413
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5413
XmaI C'CCGG_G
Cuts at: 1870 2023 2738 1870
Size: 153 715 4545
Fragments arranged by size:
4545 715 153
XmnI GAAnn'nnTTC
Cuts at: 3542 3542
Size: 5413
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3221 276
Size: 53 83 186 936 1687 2468
Fragments arranged by size:
2468 1687 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacII SalI SanDI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SexAI SfiI SfoI SrfI SwaI
XcmI