Restriction map for
pFN21AE1727
(Circular) (Six-base) MAPSORT of: pFN21AE1727.seq Check: 3797 from: 1 to: 5413
pFN21AE1727 5413 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3223 278
Size: 53 83 186 936 1687 2468
Fragments arranged by size:
2468 1687 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2288 2756 1937
Size: 351 468 4594
Fragments arranged by size:
4594 468 351
Acc65I G'GTAC_C
Cuts at: 2734 2734
Size: 5413
AclI AA'CG_TT
Cuts at: 1563 3540 3913 1563
Size: 1977 373 3063
Fragments arranged by size:
3063 1977 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2112 3476 4860 5128 1632
Size: 480 1364 1384 268 1917
Fragments arranged by size:
1917 1384 1364 480 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5216
AflIII A'CryG_T
Cuts at: 1203 2329 4312 5289 1203
Size: 1126 1983 977 1327
Fragments arranged by size:
1983 1327 1126 977
AgeI A'CCGG_T
Cuts at: 4244 4244
Size: 5413
AhdI GACnn_n'nnGTC
Cuts at: 4142 4142
Size: 5413
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5413
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5413
AlwNI CAG_nnn'CTG
Cuts at: 2392 3156 4728 2392
Size: 764 1572 3077
Fragments arranged by size:
3077 1572 764
ApaLI G'TGCA_C
Cuts at: 2601 3470 4626 2601
Size: 869 1156 3388
Fragments arranged by size:
3388 1156 869
ApoI r'AATT_y
Cuts at: 1493 2612 2722 2948 3100 5209 1493
Size: 1119 110 226 152 2109 1697
Fragments arranged by size:
2109 1697 1119 226 152 110
AseI AT'TA_AT
Cuts at: 160 1032 3967 160
Size: 872 2935 1606
Fragments arranged by size:
2935 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5413
AvaI C'yCGr_G
Cuts at: 1870 1943 2738 1870
Size: 73 795 4545
Fragments arranged by size:
4545 795 73
BamHI G'GATC_C
Cuts at: 2743 3110 2743
Size: 367 5046
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2734 4189 618
Size: 325 217 627 947 1455 1842
Fragments arranged by size:
1842 1455 947 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2732 1391
Size: 1341 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2147 2169 928
Size: 971 248 22 4172
Fragments arranged by size:
4172 971 248 22
BbvCI CC'TCA_GC
Cuts at: 2433 2433
Size: 5413
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3604 3638 1903
Size: 34 1667 34 3678
Fragments arranged by size:
3678 1667 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3305 4515 3305
Size: 1210 4203
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5413
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4024 136
Size: 107 122 71 631 605 2352 1525
Fragments arranged by size:
2352 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2104 5408 2104
Size: 3304 2109
BlpI GC'TnA_GC
Cuts at: 2830 2830
Size: 5413
Bme1580I G_kGCm'C
Cuts at: 1790 2605 3474 4630 1790
Size: 815 869 1156 2573
Fragments arranged by size:
2573 1156 869 815
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5413
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3144 4102 442
Size: 416 53 83 399 24 1727 958 1753
Fragments arranged by size:
1753 1727 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5413
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4073 1239
Size: 2834 2579
Bpu10I CC'TnA_GC
Cuts at: 2433 2433
Size: 5413
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3538 4403 4701 3538
Size: 865 298 4250
Fragments arranged by size:
4250 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4076 882
Size: 617 2577 2219
Fragments arranged by size:
2577 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3204 493
Size: 711 2000 2702
Fragments arranged by size:
2702 2000 711
BsaBI GATnn'nnATC
Cuts at: 5407 5407
Size: 5413
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3220 3602 4237 275
Size: 53 83 186 936 1687 382 635 1451
Fragments arranged by size:
1687 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2015 3846 4244 4518 4665 5076 2015
Size: 1831 398 274 147 411 2352
Fragments arranged by size:
2352 1831 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2223 2253 1178
Size: 30 165 30 820 30 4338
Fragments arranged by size:
4338 820 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5413
BseYI C'CCAG_C
Cuts at: 4616 5112 4616
Size: 496 4917
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2234 1163
Size: 573 498 4342
Fragments arranged by size:
4342 573 498
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2863 3624 4652 1540
Size: 275 186 862 761 1028 2301
Fragments arranged by size:
2301 1028 862 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2605 3474 3559 4630 2605
Size: 869 85 1071 3388
Fragments arranged by size:
3388 1071 869 85
BsmI GAATG_Cn'
Cuts at: 2924 3017 2924
Size: 93 5320
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2605 2732 3474 3559 4630 1391
Size: 399 815 127 742 85 1071 2174
Fragments arranged by size:
2174 1071 815 742 399 127 85
BspEI T'CCGG_A
Cuts at: 2015 5076 2015
Size: 3061 2352
BspHI T'CATG_A
Cuts at: 2581 3302 5032 2581
Size: 721 1730 2962
Fragments arranged by size:
2962 1730 721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2768 844
Size: 1924 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3300 1394
Size: 429 1477 3507
Fragments arranged by size:
3507 1477 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3908 4082 65
Size: 1183 2660 174 1396
Fragments arranged by size:
2660 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4057 4244 1700
Size: 110 2247 187 2869
Fragments arranged by size:
2869 2247 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5413
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5413
BssSI C'ACGA_G
Cuts at: 3473 4485 3473
Size: 1012 4401
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2221 794
Size: 1427 3986
BstBI TT'CG_AA
Cuts at: 4217 4217
Size: 5413
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5413
BstYI r'GATC_y
Cuts at: 1972 2104 2743 3110 3495 3512 4964 5050 5062
Size: 132 639 367 385 17 1452 86 12
Cuts at: 5062 5408 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 639 385 367 346 132 86
17 12
BstZ17I GTA'TAC
Cuts at: 2289 2289
Size: 5413
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3922
Fragments arranged by size:
3922 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4674
Fragments arranged by size:
4674 683 56
BtsI GCAGTG_nn'
Cuts at: 2414 2929 3723 3743 2414
Size: 515 794 20 4084
Fragments arranged by size:
4084 794 515 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5413
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5378
DraI TTT'AAA
Cuts at: 2617 2717 2617
Size: 100 5313
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4420 809
Size: 507 3104 1802
Fragments arranged by size:
3104 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2860 3749 5378
Size: 54 1425 50 275 18 1030 889 1629
Cuts at: 5378 8
Size: 43
Fragments arranged by size:
1629 1425 1030 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2860 1537
Size: 275 1048 4090
Fragments arranged by size:
4090 1048 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2262 2559 2700 3343 1353
Size: 909 297 141 643 3423
Fragments arranged by size:
3423 909 643 297 141
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4000 4374 4520 387
Size: 1064 138 2411 374 146 1280
Fragments arranged by size:
2411 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2112 3476 4073 4860 5128 1239
Size: 393 480 1364 597 787 268 1524
Fragments arranged by size:
1524 1364 787 597 480 393 268
EcoRI G'AATT_C
Cuts at: 2722 3100 2722
Size: 378 5035
FspI TGC'GCA
Cuts at: 3919 3919
Size: 5413
HaeII r_GCGC'y
Cuts at: 1678 4560 1678
Size: 2882 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2644 2676 4054 4086 4128
Size: 32 135 32 1072 32 1378 32 42
Cuts at: 4128 4160 1373
Size: 32 2626
Fragments arranged by size:
2626 1378 1072 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2242 2757 3003 669
Size: 988 281 304 515 246 3079
Fragments arranged by size:
3079 988 515 304 281 246
HindIII A'AGCT_T
Cuts at: 748 2363 748
Size: 1615 3798
HpaI GTT'AAC
Cuts at: 3003 3003
Size: 5413
KpnI G_GTAC'C
Cuts at: 2738 2738
Size: 5413
MfeI C'AATT_G
Cuts at: 3012 3012
Size: 5413
MluI A'CGCG_T
Cuts at: 5289 5289
Size: 5413
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2215 2550 4527 4711 1305
Size: 910 335 1977 184 2007
Fragments arranged by size:
2007 1977 910 335 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3591
Fragments arranged by size:
3591 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3371 3730 3889 518
Size: 648 2205 359 159 2042
Fragments arranged by size:
2205 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2829 3506 4654 4899 1606
Size: 1223 677 1148 245 2120
Fragments arranged by size:
2120 1223 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3922
Fragments arranged by size:
3922 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5413
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5413
NotI GC'GGCC_GC
Cuts at: 2860 2860
Size: 5413
NspI r_CATG'y
Cuts at: 1132 1229 2333 2771 4316 1132
Size: 97 1104 438 1545 2229
Fragments arranged by size:
2229 1545 1104 438 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5413
PciI A'CATG_T
Cuts at: 2329 4312 2329
Size: 1983 3430
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5413
PfoI T'CCnGG_A
Cuts at: 2030 2030
Size: 5413
PmeI GTTT'AAAC
Cuts at: 2717 2717
Size: 5413
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3482 3514 4995 5027 3482
Size: 32 1481 32 3868
Fragments arranged by size:
3868 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2147 1613
Size: 534 4879
PsiI TTA'TAA
Cuts at: 2983 2983
Size: 5413
PstI C_TGCA'G
Cuts at: 830 2149 2185 2765 830
Size: 1319 36 580 3478
Fragments arranged by size:
3478 1319 580 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5413
SalI G'TCGA_C
Cuts at: 1936 2755 1936
Size: 819 4594
SapI GCTCTTCn'nnn_
Cuts at: 2262 2262
Size: 5413
SbfI CC_TGCA'GG
Cuts at: 2765 2765
Size: 5413
ScaI AGT'ACT
Cuts at: 3661 5373 3661
Size: 1712 3701
SfcI C'TryA_G
Cuts at: 826 1045 2145 2181 2761 3896 4577 4768 826
Size: 219 1100 36 580 1135 681 191 1471
Fragments arranged by size:
1471 1135 1100 681 580 219 191 36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5413
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5413
SmaI CCC'GGG
Cuts at: 1872 2740 1872
Size: 868 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3517 4418 4680 820
Size: 197 926 1574 901 262 1553
Fragments arranged by size:
1574 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3204 493
Size: 2711 2702
SpeI A'CTAG_T
Cuts at: 152 2309 152
Size: 2157 3256
SphI G_CATG'C
Cuts at: 1132 2771 1132
Size: 1639 3774
SspI AAT'ATT
Cuts at: 5 52 2378 3337 5
Size: 47 2326 959 2081
Fragments arranged by size:
2326 2081 959 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5413
StyI C'CwwG_G
Cuts at: 513 1065 2004 2292 2852 513
Size: 552 939 288 560 3074
Fragments arranged by size:
3074 939 560 552 288
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3440 3457 3610 3795 4240 325
Size: 1255 1860 17 153 185 445 1498
Fragments arranged by size:
1860 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3659 5371 96
Size: 274 80 33 51 1443 1682 1712 138
Fragments arranged by size:
1712 1682 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5373 5405 5373
Size: 32 5381
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5413
XbaI T'CTAG_A
Cuts at: 2495 2749 2495
Size: 254 5159
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5413
XmaI C'CCGG_G
Cuts at: 1870 2738 1870
Size: 868 4545
XmnI GAAnn'nnTTC
Cuts at: 3542 3542
Size: 5413
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3221 276
Size: 53 83 186 936 1687 2468
Fragments arranged by size:
2468 1687 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI