Restriction map for
pFN21AE1720
(Circular) (Six-base) MAPSORT of: pFN21AE1720.seq Check: 5511 from: 1 to: 5386
pFN21AE1720 5386 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3196 278
Size: 53 83 186 936 1660 2468
Fragments arranged by size:
2468 1660 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2729 1937
Size: 792 4594
Acc65I G'GTAC_C
Cuts at: 2707 2707
Size: 5386
AclI AA'CG_TT
Cuts at: 1563 3513 3886 1563
Size: 1950 373 3063
Fragments arranged by size:
3063 1950 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2393 2456 2592 3449 4833 5101 1632
Size: 761 63 136 857 1384 268 1917
Fragments arranged by size:
1917 1384 857 761 268 136 63
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5189
AflIII A'CryG_T
Cuts at: 1203 4285 5262 1203
Size: 3082 977 1327
Fragments arranged by size:
3082 1327 977
AgeI A'CCGG_T
Cuts at: 4217 4217
Size: 5386
AhdI GACnn_n'nnGTC
Cuts at: 4115 4115
Size: 5386
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5386
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5386
AlwNI CAG_nnn'CTG
Cuts at: 3129 4701 3129
Size: 1572 3814
ApaLI G'TGCA_C
Cuts at: 3443 4599 3443
Size: 1156 4230
ApoI r'AATT_y
Cuts at: 1493 2695 2921 3073 5182 1493
Size: 1202 226 152 2109 1697
Fragments arranged by size:
2109 1697 1202 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3940 160
Size: 872 2908 1606
Fragments arranged by size:
2908 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5386
AvaI C'yCGr_G
Cuts at: 1870 1943 2248 2517 2711 1870
Size: 73 305 269 194 4545
Fragments arranged by size:
4545 305 269 194 73
BamHI G'GATC_C
Cuts at: 2290 2716 3083 2290
Size: 426 367 4593
Fragments arranged by size:
4593 426 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2363 2707 4162 618
Size: 325 217 627 576 344 1455 1842
Fragments arranged by size:
1842 1455 627 576 344 325 217
BanII G_rGCy'C
Cuts at: 1391 2652 2705 1391
Size: 1261 53 4072
Fragments arranged by size:
4072 1261 53
BbeI G_GCGC'C
Cuts at: 2367 2367
Size: 5386
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4415
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3577 3611 1903
Size: 34 1640 34 3678
Fragments arranged by size:
3678 1640 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3278 4488 3278
Size: 1210 4176
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5386
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2102 2414 3997
Size: 107 122 71 631 605 430 312 1583
Cuts at: 3997 136
Size: 1525
Fragments arranged by size:
1583 1525 631 605 430 312 122 107
71
BglII A'GATC_T
Cuts at: 5381 5381
Size: 5386
BlpI GC'TnA_GC
Cuts at: 2803 2803
Size: 5386
Bme1580I G_kGCm'C
Cuts at: 1790 2098 3447 4603 1790
Size: 308 1349 1156 2573
Fragments arranged by size:
2573 1349 1156 308
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5386
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2054 2578 3117
Size: 416 53 83 399 24 637 524 539
Cuts at: 3117 4075 442
Size: 958 1753
Fragments arranged by size:
1753 958 637 539 524 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5386
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2088 2353 2606 4046 1239
Size: 849 265 253 1440 2579
Fragments arranged by size:
2579 1440 849 265 253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3511 4376 4674 3511
Size: 865 298 4223
Fragments arranged by size:
4223 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2054 4049 882
Size: 617 555 1995 2219
Fragments arranged by size:
2219 1995 617 555
BsaAI yAC'GTr
Cuts at: 493 1204 3177 493
Size: 711 1973 2702
Fragments arranged by size:
2702 1973 711
BsaBI GATnn'nnATC
Cuts at: 5380 5380
Size: 5386
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2364 3193 3575 4210
Size: 53 83 186 936 831 829 382 635
Cuts at: 4210 275
Size: 1451
Fragments arranged by size:
1451 936 831 829 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3819 4217 4491 4638 5049 3819
Size: 398 274 147 411 4156
Fragments arranged by size:
4156 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2604 2634 1178
Size: 30 165 30 1201 30 3930
Fragments arranged by size:
3930 1201 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5386
BseYI C'CCAG_C
Cuts at: 4589 5085 4589
Size: 496 4890
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4813
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2836 3597 4625 1540
Size: 275 186 835 761 1028 2301
Fragments arranged by size:
2301 1028 835 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3447 3532 4603 3447
Size: 85 1071 4230
Fragments arranged by size:
4230 1071 85
BsmI GAATG_Cn'
Cuts at: 2897 2990 2897
Size: 93 5293
BsmBI CGTCTCn'nnnn_
Cuts at: 2064 2064
Size: 5386
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2098 2652 2705 3447 3532 4603 1391
Size: 399 308 554 53 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 554 399 308 85 53
BspEI T'CCGG_A
Cuts at: 5049 5049
Size: 5386
BspHI T'CATG_A
Cuts at: 2052 2589 3275 5005 2052
Size: 537 686 1730 2433
Fragments arranged by size:
2433 1730 686 537
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2741 844
Size: 1897 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3273 1394
Size: 429 1450 3507
Fragments arranged by size:
3507 1450 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3881 4055 65
Size: 1183 2633 174 1396
Fragments arranged by size:
2633 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2428 4030 4217 1700
Size: 110 618 1602 187 2869
Fragments arranged by size:
2869 1602 618 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5386
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5386
BssSI C'ACGA_G
Cuts at: 3446 4458 3446
Size: 1012 4374
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5386
BstBI TT'CG_AA
Cuts at: 4190 4190
Size: 5386
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5386
BstYI r'GATC_y
Cuts at: 1972 2290 2716 3083 3468 3485 4937 5023 5035
Size: 318 426 367 385 17 1452 86 12
Cuts at: 5035 5381 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 426 385 367 346 318 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2256 513
Size: 552 939 252 3643
Fragments arranged by size:
3643 939 552 252
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2432 487
Size: 683 56 1206 3441
Fragments arranged by size:
3441 1206 683 56
BtsI GCAGTG_nn'
Cuts at: 2902 3696 3716 2902
Size: 794 20 4572
Fragments arranged by size:
4572 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5386
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5351
DraI TTT'AAA
Cuts at: 2690 2690
Size: 5386
DraIII CAC_nnn'GTG
Cuts at: 2438 2438
Size: 5386
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4393 809
Size: 507 3077 1802
Fragments arranged by size:
3077 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2833 3722 5351
Size: 54 1425 50 275 18 1003 889 1629
Cuts at: 5351 8
Size: 43
Fragments arranged by size:
1629 1425 1003 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2833 1537
Size: 275 1021 4090
Fragments arranged by size:
4090 1021 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3316 1353
Size: 1963 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2684 3973 4347 4493 387
Size: 1064 138 1095 1289 374 146 1280
Fragments arranged by size:
1289 1280 1095 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2088 2353 2393 2456 2592 2606 3449
Size: 393 456 265 40 63 136 14 843
Cuts at: 3449 4046 4833 5101 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 843 787 597 456 393 268 265
136 63 40 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2501 2501
Size: 5386
EcoO109I rG'GnC_Cy
Cuts at: 2104 2137 2104
Size: 33 5353
EcoRI G'AATT_C
Cuts at: 2695 3073 2695
Size: 378 5008
EcoRV GAT'ATC
Cuts at: 2356 2356
Size: 5386
FspI TGC'GCA
Cuts at: 3892 3892
Size: 5386
HaeII r_GCGC'y
Cuts at: 1678 2367 4533 1678
Size: 689 2166 2531
Fragments arranged by size:
2531 2166 689
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2199 2231 2275 2307 4027
Size: 32 135 32 627 32 44 32 1720
Cuts at: 4027 4059 4101 4133 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1720 627 135 44 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2730 2976 669
Size: 988 281 792 246 3079
Fragments arranged by size:
3079 988 792 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5386
HpaI GTT'AAC
Cuts at: 2976 2976
Size: 5386
KasI G'GCGC_C
Cuts at: 2363 2363
Size: 5386
KpnI G_GTAC'C
Cuts at: 2711 2711
Size: 5386
MfeI C'AATT_G
Cuts at: 2985 2985
Size: 5386
MluI A'CGCG_T
Cuts at: 5262 5262
Size: 5386
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1991 4500 4684 1305
Size: 686 2509 184 2007
Fragments arranged by size:
2509 2007 686 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3564
Fragments arranged by size:
3564 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2057 3344 3703 3862 518
Size: 648 891 1287 359 159 2042
Fragments arranged by size:
2042 1287 891 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2802 3479 4627 4872 1606
Size: 1196 677 1148 245 2120
Fragments arranged by size:
2120 1196 1148 677 245
NarI GG'CG_CC
Cuts at: 2364 2364
Size: 5386
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3895
Fragments arranged by size:
3895 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5386
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5386
NotI GC'GGCC_GC
Cuts at: 2833 2833
Size: 5386
NspI r_CATG'y
Cuts at: 1132 1229 2744 4289 1132
Size: 97 1515 1545 2229
Fragments arranged by size:
2229 1545 1515 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5386
PciI A'CATG_T
Cuts at: 4285 4285
Size: 5386
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4484
PfoI T'CCnGG_A
Cuts at: 2222 2222
Size: 5386
PmeI GTTT'AAAC
Cuts at: 2690 2690
Size: 5386
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3455 3487 4968 5000 3455
Size: 32 1481 32 3841
Fragments arranged by size:
3841 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5386
PsiI TTA'TAA
Cuts at: 2956 2956
Size: 5386
PspXI vC'TCGA_Gb
Cuts at: 2517 2517
Size: 5386
PstI C_TGCA'G
Cuts at: 830 2738 830
Size: 1908 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5386
SalI G'TCGA_C
Cuts at: 1936 2728 1936
Size: 792 4594
SbfI CC_TGCA'GG
Cuts at: 2738 2738
Size: 5386
ScaI AGT'ACT
Cuts at: 3634 5346 3634
Size: 1712 3674
SfcI C'TryA_G
Cuts at: 826 1045 2203 2734 3869 4550 4741 826
Size: 219 1158 531 1135 681 191 1471
Fragments arranged by size:
1471 1158 1135 681 531 219 191
SfoI GGC'GCC
Cuts at: 2365 2365
Size: 5386
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5386
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5386
SmaI CCC'GGG
Cuts at: 1872 2250 2713 1872
Size: 378 463 4545
Fragments arranged by size:
4545 463 378
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2517 3490 4391 4653 820
Size: 197 926 574 973 901 262 1553
Fragments arranged by size:
1553 973 926 901 574 262 197
SnaBI TAC'GTA
Cuts at: 493 3177 493
Size: 2684 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5386
SphI G_CATG'C
Cuts at: 1132 2744 1132
Size: 1612 3774
SspI AAT'ATT
Cuts at: 5 52 3310 5
Size: 47 3258 2081
Fragments arranged by size:
3258 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5386
StyI C'CwwG_G
Cuts at: 513 1065 2004 2502 2825 513
Size: 552 939 498 323 3074
Fragments arranged by size:
3074 939 552 498 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2597 3413 3430 3583 3768 4213 325
Size: 1255 1017 816 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1017 816 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3632 5344 96
Size: 274 80 33 51 1443 1655 1712 138
Fragments arranged by size:
1712 1655 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2604 2636 5346 5378 2604
Size: 32 2710 32 2612
Fragments arranged by size:
2710 2612 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5386
XbaI T'CTAG_A
Cuts at: 2722 2722
Size: 5386
XhoI C'TCGA_G
Cuts at: 1943 2517 1943
Size: 574 4812
XmaI C'CCGG_G
Cuts at: 1870 2248 2711 1870
Size: 378 463 4545
Fragments arranged by size:
4545 463 378
XmnI GAAnn'nnTTC
Cuts at: 2552 3515 2552
Size: 963 4423
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3194 276
Size: 53 83 186 936 1660 2468
Fragments arranged by size:
2468 1660 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI