Restriction map for
pFN21AE1717
(Circular) (Six-base) MAPSORT of: pFN21AE1717.seq Check: 9336 from: 1 to: 5374
pFN21AE1717 5374 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3184 278
Size: 53 83 186 936 1648 2468
Fragments arranged by size:
2468 1648 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2274 2717 1937
Size: 337 443 4594
Fragments arranged by size:
4594 443 337
Acc65I G'GTAC_C
Cuts at: 2695 2695
Size: 5374
AclI AA'CG_TT
Cuts at: 1563 3501 3874 1563
Size: 1938 373 3063
Fragments arranged by size:
3063 1938 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3437 4821 5089 1632
Size: 1805 1384 268 1917
Fragments arranged by size:
1917 1805 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5177
AflIII A'CryG_T
Cuts at: 1203 4273 5250 1203
Size: 3070 977 1327
Fragments arranged by size:
3070 1327 977
AgeI A'CCGG_T
Cuts at: 4205 4205
Size: 5374
AhdI GACnn_n'nnGTC
Cuts at: 4103 4103
Size: 5374
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5374
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5374
AlwNI CAG_nnn'CTG
Cuts at: 2440 3117 4689 2440
Size: 677 1572 3125
Fragments arranged by size:
3125 1572 677
ApaLI G'TGCA_C
Cuts at: 3431 4587 3431
Size: 1156 4218
ApoI r'AATT_y
Cuts at: 1493 2683 2909 3061 5170 1493
Size: 1190 226 152 2109 1697
Fragments arranged by size:
2109 1697 1190 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3928 160
Size: 872 2896 1606
Fragments arranged by size:
2896 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5374
AvaI C'yCGr_G
Cuts at: 1870 1943 2699 1870
Size: 73 756 4545
Fragments arranged by size:
4545 756 73
BamHI G'GATC_C
Cuts at: 2403 2704 3071 2403
Size: 301 367 4706
Fragments arranged by size:
4706 367 301
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2695 4150 618
Size: 325 217 627 908 1455 1842
Fragments arranged by size:
1842 1455 908 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2361 2693 1391
Size: 970 332 4072
Fragments arranged by size:
4072 970 332
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2031 928
Size: 971 132 4271
Fragments arranged by size:
4271 971 132
BbvCI CC'TCA_GC
Cuts at: 2600 2600
Size: 5374
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3565 3599 1903
Size: 34 1628 34 3678
Fragments arranged by size:
3678 1628 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3266 4476 3266
Size: 1210 4164
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5374
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2484 3985 136
Size: 107 122 71 631 605 812 1501 1525
Fragments arranged by size:
1525 1501 812 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2643 5369 2643
Size: 2726 2648
BlpI GC'TnA_GC
Cuts at: 2791 2791
Size: 5374
Bme1580I G_kGCm'C
Cuts at: 1790 2200 3435 4591 1790
Size: 410 1235 1156 2573
Fragments arranged by size:
2573 1235 1156 410
BmgBI CAC'GTC
Cuts at: 1318 2395 2539 2569 1318
Size: 1077 144 30 4123
Fragments arranged by size:
4123 1077 144 30
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3105 4063 442
Size: 416 53 83 399 24 1688 958 1753
Fragments arranged by size:
1753 1688 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5374
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2401 2401
Size: 5374
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2101 2345 4034 1239
Size: 862 244 1689 2579
Fragments arranged by size:
2579 1689 862 244
Bpu10I CC'TnA_GC
Cuts at: 2600 2600
Size: 5374
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3499 4364 4662 3499
Size: 865 298 4211
Fragments arranged by size:
4211 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4037 882
Size: 617 2538 2219
Fragments arranged by size:
2538 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3165 493
Size: 711 1961 2702
Fragments arranged by size:
2702 1961 711
BsaBI GATnn'nnATC
Cuts at: 2402 5368 2402
Size: 2966 2408
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3181 3563 4198 275
Size: 53 83 186 936 1648 382 635 1451
Fragments arranged by size:
1648 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3807 4205 4479 4626 5037 3807
Size: 398 274 147 411 4144
Fragments arranged by size:
4144 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5149
Fragments arranged by size:
5149 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2336 2630 1186
Size: 1150 294 3930
Fragments arranged by size:
3930 1150 294
BseYI C'CCAG_C
Cuts at: 4577 5073 4577
Size: 496 4878
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2613 1163
Size: 573 877 3924
Fragments arranged by size:
3924 877 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2824 3585 4613 1540
Size: 275 186 823 761 1028 2301
Fragments arranged by size:
2301 1028 823 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3435 3520 4591 3435
Size: 85 1071 4218
Fragments arranged by size:
4218 1071 85
BsmI GAATG_Cn'
Cuts at: 2885 2978 2885
Size: 93 5281
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2200 2361 2693 3435 3520 4591 1391
Size: 399 410 161 332 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 410 399 332 161 85
BspEI T'CCGG_A
Cuts at: 5037 5037
Size: 5374
BspHI T'CATG_A
Cuts at: 3263 4993 3263
Size: 1730 3644
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2729 844
Size: 1885 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3261 1394
Size: 429 1438 3507
Fragments arranged by size:
3507 1438 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3869 4043 65
Size: 1183 2621 174 1396
Fragments arranged by size:
2621 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4018 4205 1700
Size: 110 2208 187 2869
Fragments arranged by size:
2869 2208 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5374
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5374
BssSI C'ACGA_G
Cuts at: 3434 4446 3434
Size: 1012 4362
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2553 794
Size: 1759 3615
BstBI TT'CG_AA
Cuts at: 4178 4178
Size: 5374
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5374
BstYI r'GATC_y
Cuts at: 1972 2403 2643 2704 3071 3456 3473 4925 5011
Size: 431 240 61 367 385 17 1452 86
Cuts at: 5011 5023 5369 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 431 385 367 346 240 86
61 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2079 513
Size: 552 1014 3808
Fragments arranged by size:
3808 1014 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4635
Fragments arranged by size:
4635 683 56
BtsI GCAGTG_nn'
Cuts at: 2596 2890 3684 3704 2596
Size: 294 794 20 4266
Fragments arranged by size:
4266 794 294 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5374
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5339
DraI TTT'AAA
Cuts at: 2678 2678
Size: 5374
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2188 4381 809
Size: 507 872 2193 1802
Fragments arranged by size:
2193 1802 872 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2107 2442 2610
Size: 54 1425 50 275 18 277 335 168
Cuts at: 2610 2821 3710 5339 8
Size: 211 889 1629 43
Fragments arranged by size:
1629 1425 889 335 277 275 211 168
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2821 1537
Size: 275 1009 4090
Fragments arranged by size:
4090 1009 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3304 1353
Size: 1951 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2465 3961 4335 4481 387
Size: 1064 138 876 1496 374 146 1280
Fragments arranged by size:
1496 1280 1064 876 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2101 2345 3437 4034 4821 5089 1239
Size: 393 469 244 1092 597 787 268 1524
Fragments arranged by size:
1524 1092 787 597 469 393 268 244
EcoNI CCTnn'n_nnAGG
Cuts at: 2318 2612 2318
Size: 294 5080
EcoO109I rG'GnC_Cy
Cuts at: 2560 2560
Size: 5374
EcoRI G'AATT_C
Cuts at: 2683 3061 2683
Size: 378 4996
FspI TGC'GCA
Cuts at: 3880 3880
Size: 5374
HaeII r_GCGC'y
Cuts at: 1678 2461 4521 1678
Size: 783 2060 2531
Fragments arranged by size:
2531 2060 783
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4015 4047 4089 4121 1373
Size: 32 135 32 2443 32 42 32 2626
Fragments arranged by size:
2626 2443 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2275 2718 2964 669
Size: 988 281 337 443 246 3079
Fragments arranged by size:
3079 988 443 337 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5374
HpaI GTT'AAC
Cuts at: 2964 2964
Size: 5374
KpnI G_GTAC'C
Cuts at: 2699 2699
Size: 5374
MfeI C'AATT_G
Cuts at: 2973 2973
Size: 5374
MluI A'CGCG_T
Cuts at: 5250 5250
Size: 5374
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4488 4672 1305
Size: 3183 184 2007
Fragments arranged by size:
3183 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2109 10
Size: 54 1425 343 277 3275
Fragments arranged by size:
3275 1425 343 277 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3332 3691 3850 518
Size: 648 2166 359 159 2042
Fragments arranged by size:
2166 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2251 2790 3467 4615 4860 1606
Size: 645 539 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 645 539 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4822
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5374
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5374
NotI GC'GGCC_GC
Cuts at: 2821 2821
Size: 5374
NspI r_CATG'y
Cuts at: 1132 1229 2095 2732 4277 1132
Size: 97 866 637 1545 2229
Fragments arranged by size:
2229 1545 866 637 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5374
PciI A'CATG_T
Cuts at: 4273 4273
Size: 5374
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5374
PfoI T'CCnGG_A
Cuts at: 2138 2361 2138
Size: 223 5151
PmeI GTTT'AAAC
Cuts at: 2678 2678
Size: 5374
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3443 3475 4956 4988 3443
Size: 32 1481 32 3829
Fragments arranged by size:
3829 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2560 2560
Size: 5374
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5374
PsiI TTA'TAA
Cuts at: 2944 2944
Size: 5374
PstI C_TGCA'G
Cuts at: 830 2154 2591 2726 830
Size: 1324 437 135 3478
Fragments arranged by size:
3478 1324 437 135
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5374
SalI G'TCGA_C
Cuts at: 1936 2273 2716 1936
Size: 337 443 4594
Fragments arranged by size:
4594 443 337
SbfI CC_TGCA'GG
Cuts at: 2726 2726
Size: 5374
ScaI AGT'ACT
Cuts at: 3622 5334 3622
Size: 1712 3662
SfcI C'TryA_G
Cuts at: 826 1045 2150 2587 2656 2722 3857 4538 4729
Size: 219 1105 437 69 66 1135 681 191
Cuts at: 4729 826
Size: 1471
Fragments arranged by size:
1471 1135 1105 681 437 219 191 69
66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5374
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5374
SmaI CCC'GGG
Cuts at: 1872 2701 1872
Size: 829 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3478 4379 4641 820
Size: 197 926 1535 901 262 1553
Fragments arranged by size:
1553 1535 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3165 493
Size: 2672 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5374
SphI G_CATG'C
Cuts at: 1132 2095 2732 1132
Size: 963 637 3774
Fragments arranged by size:
3774 963 637
SspI AAT'ATT
Cuts at: 5 52 3298 5
Size: 47 3246 2081
Fragments arranged by size:
3246 2081 47
StuI AGG'CCT
Cuts at: 1517 2598 1517
Size: 1081 4293
StyI C'CwwG_G
Cuts at: 513 1065 2046 2813 513
Size: 552 981 767 3074
Fragments arranged by size:
3074 981 767 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2092 2401 3401 3418 3571 3756 4201
Size: 1255 512 309 1000 17 153 185 445
Cuts at: 4201 325
Size: 1498
Fragments arranged by size:
1498 1255 1000 512 445 309 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3620 5332 96
Size: 274 80 33 51 1443 1643 1712 138
Fragments arranged by size:
1712 1643 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5334 5366 5334
Size: 32 5342
Tth111I GACn'n_nGTC
Cuts at: 1316 2570 1316
Size: 1254 4120
XbaI T'CTAG_A
Cuts at: 2710 2710
Size: 5374
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2311 2311
Size: 5374
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5374
XmaI C'CCGG_G
Cuts at: 1870 2699 1870
Size: 829 4545
XmnI GAAnn'nnTTC
Cuts at: 2036 2345 3503 2036
Size: 309 1158 3907
Fragments arranged by size:
3907 1158 309
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3182 276
Size: 53 83 186 936 1648 2468
Fragments arranged by size:
2468 1648 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI