Restriction map for
pFN21AE1702
(Circular) (Six-base) MAPSORT of: pFN21AE1702.seq Check: 4996 from: 1 to: 5317
pFN21AE1702 5317 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2424 3127 278
Size: 53 83 186 936 888 703 2468
Fragments arranged by size:
2468 936 888 703 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2660 1937
Size: 723 4594
Acc65I G'GTAC_C
Cuts at: 2638 2638
Size: 5317
AclI AA'CG_TT
Cuts at: 1563 3444 3817 1563
Size: 1881 373 3063
Fragments arranged by size:
3063 1881 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3380 4764 5032 1632
Size: 1748 1384 268 1917
Fragments arranged by size:
1917 1748 1384 268
AfeI AGC'GCT
Cuts at: 2184 2184
Size: 5317
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5120
AflIII A'CryG_T
Cuts at: 1203 4216 5193 1203
Size: 3013 977 1327
Fragments arranged by size:
3013 1327 977
AgeI A'CCGG_T
Cuts at: 4148 4148
Size: 5317
AhdI GACnn_n'nnGTC
Cuts at: 4046 4046
Size: 5317
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5317
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5317
AlwNI CAG_nnn'CTG
Cuts at: 2056 2299 2368 3060 4632 2056
Size: 243 69 692 1572 2741
Fragments arranged by size:
2741 1572 692 243 69
ApaI G_GGCC'C
Cuts at: 2108 2108
Size: 5317
ApaLI G'TGCA_C
Cuts at: 2070 3374 4530 2070
Size: 1304 1156 2857
Fragments arranged by size:
2857 1304 1156
ApoI r'AATT_y
Cuts at: 1493 2626 2852 3004 5113 1493
Size: 1133 226 152 2109 1697
Fragments arranged by size:
2109 1697 1133 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3871 160
Size: 872 2839 1606
Fragments arranged by size:
2839 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5317
AvaI C'yCGr_G
Cuts at: 1870 1943 2225 2384 2642 1870
Size: 73 282 159 258 4545
Fragments arranged by size:
4545 282 258 159 73
BamHI G'GATC_C
Cuts at: 2647 3014 2647
Size: 367 4950
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2498 2638 4093 618
Size: 325 217 627 711 140 1455 1842
Fragments arranged by size:
1842 1455 711 627 325 217 140
BanII G_rGCy'C
Cuts at: 1391 2030 2108 2272 2358 2636 1391
Size: 639 78 164 86 278 4072
Fragments arranged by size:
4072 639 278 164 86 78
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4346
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3508 3542 1903
Size: 34 1571 34 3678
Fragments arranged by size:
3678 1571 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3209 4419 3209
Size: 1210 4107
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5317
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2507 3928 136
Size: 107 122 71 631 605 835 1421 1525
Fragments arranged by size:
1525 1421 835 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5312 5312
Size: 5317
BlpI GC'TnA_GC
Cuts at: 2734 2734
Size: 5317
Bme1580I G_kGCm'C
Cuts at: 1790 2074 2108 3378 4534 1790
Size: 284 34 1270 1156 2573
Fragments arranged by size:
2573 1270 1156 284 34
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5317
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2474 3048 4006
Size: 416 53 83 399 24 1057 574 958
Cuts at: 4006 442
Size: 1753
Fragments arranged by size:
1753 1057 958 574 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5317
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2171 2597 3977 1239
Size: 932 426 1380 2579
Fragments arranged by size:
2579 1380 932 426
Bpu10I CC'TnA_GC
Cuts at: 2276 2276
Size: 5317
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2123 2338 3442 4307 4605 2123
Size: 215 1104 865 298 2835
Fragments arranged by size:
2835 1104 865 298 215
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3980 882
Size: 617 2481 2219
Fragments arranged by size:
2481 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3108 493
Size: 711 1904 2702
Fragments arranged by size:
2702 1904 711
BsaBI GATnn'nnATC
Cuts at: 5311 5311
Size: 5317
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2421 3124 3506 4141
Size: 53 83 186 936 888 703 382 635
Cuts at: 4141 275
Size: 1451
Fragments arranged by size:
1451 936 888 703 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3750 4148 4422 4569 4980 3750
Size: 398 274 147 411 4087
Fragments arranged by size:
4087 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2183 2213 2570 2600 1178
Size: 30 165 30 780 30 357 30 3895
Fragments arranged by size:
3895 780 357 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2243 1186
Size: 1057 4260
BseYI C'CCAG_C
Cuts at: 2288 2610 4520 5016 2288
Size: 322 1910 496 2589
Fragments arranged by size:
2589 1910 496 322
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2105 1163
Size: 573 369 4375
Fragments arranged by size:
4375 573 369
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2767 3528 4556 1540
Size: 275 186 766 761 1028 2301
Fragments arranged by size:
2301 1028 766 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2074 3378 3463 4534 2074
Size: 1304 85 1071 2857
Fragments arranged by size:
2857 1304 1071 85
BsmI GAATG_Cn'
Cuts at: 2828 2921 2828
Size: 93 5224
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2074 2108 2272 2358 2636 3378
Size: 399 240 44 34 164 86 278 742
Cuts at: 3378 3463 4534 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 278 240 164 86
85 44 34
BspEI T'CCGG_A
Cuts at: 4980 4980
Size: 5317
BspHI T'CATG_A
Cuts at: 3206 4936 3206
Size: 1730 3587
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2023 2672 844
Size: 1179 649 3489
Fragments arranged by size:
3489 1179 649
BsrBI CCG'CTC
Cuts at: 1394 1823 3204 1394
Size: 429 1381 3507
Fragments arranged by size:
3507 1381 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3812 3986 65
Size: 1183 2564 174 1396
Fragments arranged by size:
2564 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2506 3961 4148 1700
Size: 110 696 1455 187 2869
Fragments arranged by size:
2869 1455 696 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5317
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5317
BssSI C'ACGA_G
Cuts at: 3377 4389 3377
Size: 1012 4305
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2056 794
Size: 1262 4055
BstBI TT'CG_AA
Cuts at: 4121 4121
Size: 5317
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5317
BstXI CCAn_nnnn'nTGG
Cuts at: 2101 2174 2101
Size: 73 5244
BstYI r'GATC_y
Cuts at: 1972 2163 2376 2647 3014 3399 3416 4868 4954
Size: 191 213 271 367 385 17 1452 86
Cuts at: 4954 4966 5312 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 271 213 191
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3826
Fragments arranged by size:
3826 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2187 487
Size: 683 56 961 3617
Fragments arranged by size:
3617 961 683 56
BtsI GCAGTG_nn'
Cuts at: 2408 2833 3627 3647 2408
Size: 425 794 20 4078
Fragments arranged by size:
4078 794 425 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5317
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5282
DraI TTT'AAA
Cuts at: 2621 2621
Size: 5317
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4324 809
Size: 507 3008 1802
Fragments arranged by size:
3008 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2764 3653 5282
Size: 54 1425 50 275 18 934 889 1629
Cuts at: 5282 8
Size: 43
Fragments arranged by size:
1629 1425 934 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2764 1537
Size: 275 952 4090
Fragments arranged by size:
4090 952 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3247 1353
Size: 1894 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3904 4278 4424 387
Size: 1064 138 2315 374 146 1280
Fragments arranged by size:
2315 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2171 2597 3380 3977 4764 5032 1239
Size: 393 539 426 783 597 787 268 1524
Fragments arranged by size:
1524 787 783 597 539 426 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2348 2348
Size: 5317
EcoO109I rG'GnC_Cy
Cuts at: 2105 2296 2365 2393 2545 2105
Size: 191 69 28 152 4877
Fragments arranged by size:
4877 191 152 69 28
EcoRI G'AATT_C
Cuts at: 2626 3004 2626
Size: 378 4939
FspI TGC'GCA
Cuts at: 3823 3823
Size: 5317
HaeII r_GCGC'y
Cuts at: 1678 2186 2533 4464 1678
Size: 508 347 1931 2531
Fragments arranged by size:
2531 1931 508 347
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3958 3990 4032 4064 1373
Size: 32 135 32 2386 32 42 32 2626
Fragments arranged by size:
2626 2386 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2661 2907 669
Size: 988 281 723 246 3079
Fragments arranged by size:
3079 988 723 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5317
HpaI GTT'AAC
Cuts at: 2907 2907
Size: 5317
KpnI G_GTAC'C
Cuts at: 2642 2642
Size: 5317
MfeI C'AATT_G
Cuts at: 2916 2916
Size: 5317
MluI A'CGCG_T
Cuts at: 5193 5193
Size: 5317
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4431 4615 1305
Size: 3126 184 2007
Fragments arranged by size:
3126 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3495
Fragments arranged by size:
3495 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3275 3634 3793 518
Size: 648 2109 359 159 2042
Fragments arranged by size:
2109 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2053 2283 2305 2416 2733 3410 4558 4803
Size: 447 230 22 111 317 677 1148 245
Cuts at: 4803 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 447 317 245 230 111
22
NaeI GCC'GGC
Cuts at: 2508 2508
Size: 5317
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3826
Fragments arranged by size:
3826 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5317
NgoMIV G'CCGG_C
Cuts at: 2506 2506
Size: 5317
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5317
NotI GC'GGCC_GC
Cuts at: 2764 2764
Size: 5317
NspI r_CATG'y
Cuts at: 1132 1229 2675 4220 1132
Size: 97 1446 1545 2229
Fragments arranged by size:
2229 1545 1446 97
PasI CC'CwG_GG
Cuts at: 1348 2101 2265 2494 1348
Size: 753 164 229 4171
Fragments arranged by size:
4171 753 229 164
PciI A'CATG_T
Cuts at: 4216 4216
Size: 5317
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2445 1108
Size: 1337 3980
PfoI T'CCnGG_A
Cuts at: 2187 2574 2187
Size: 387 4930
PmeI GTTT'AAAC
Cuts at: 2621 2621
Size: 5317
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3386 3418 4899 4931 3386
Size: 32 1481 32 3772
Fragments arranged by size:
3772 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2393 2545 2393
Size: 152 5165
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5317
PsiI TTA'TAA
Cuts at: 2887 2887
Size: 5317
PspOMI G'GGCC_C
Cuts at: 2104 2104
Size: 5317
PstI C_TGCA'G
Cuts at: 830 2052 2304 2364 2415 2487 2568 2669 830
Size: 1222 252 60 51 72 81 101 3478
Fragments arranged by size:
3478 1222 252 101 81 72 60 51
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5317
PvuII CAG'CTG
Cuts at: 2053 2283 2305 2416 2053
Size: 230 22 111 4954
Fragments arranged by size:
4954 230 111 22
SalI G'TCGA_C
Cuts at: 1936 2659 1936
Size: 723 4594
SanDI GG'GwC_CC
Cuts at: 2393 2393
Size: 5317
SbfI CC_TGCA'GG
Cuts at: 2364 2669 2364
Size: 305 5012
ScaI AGT'ACT
Cuts at: 3565 5277 3565
Size: 1712 3605
SfcI C'TryA_G
Cuts at: 826 1045 2048 2300 2360 2411 2483 2564 2665
Size: 219 1003 252 60 51 72 81 101
Cuts at: 2665 3800 4481 4672 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1003 681 252 219 191 101
81 72 60 51
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5317
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5317
SmaI CCC'GGG
Cuts at: 1872 2644 1872
Size: 772 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2138 2317 3421 4322 4584 820
Size: 197 926 195 179 1104 901 262 1553
Fragments arranged by size:
1553 1104 926 901 262 197 195 179
SnaBI TAC'GTA
Cuts at: 493 3108 493
Size: 2615 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5317
SphI G_CATG'C
Cuts at: 1132 2675 1132
Size: 1543 3774
SspI AAT'ATT
Cuts at: 5 52 3241 5
Size: 47 3189 2081
Fragments arranged by size:
3189 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5317
StyI C'CwwG_G
Cuts at: 513 1065 2004 2400 2756 513
Size: 552 939 396 356 3074
Fragments arranged by size:
3074 939 552 396 356
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2036 2507 3344 3361 3514 3699 4144
Size: 1255 456 471 837 17 153 185 445
Cuts at: 4144 325
Size: 1498
Fragments arranged by size:
1498 1255 837 471 456 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3563 5275 96
Size: 274 80 33 51 1443 1586 1712 138
Fragments arranged by size:
1712 1586 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2183 2215 5277 5309 2183
Size: 32 3062 32 2191
Fragments arranged by size:
3062 2191 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5317
XbaI T'CTAG_A
Cuts at: 2653 2653
Size: 5317
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2038 2038
Size: 5317
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5317
XmaI C'CCGG_G
Cuts at: 1870 2642 1870
Size: 772 4545
XmnI GAAnn'nnTTC
Cuts at: 3446 3446
Size: 5317
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2422 3125 276
Size: 53 83 186 936 888 703 2468
Fragments arranged by size:
2468 936 888 703 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SacII SapI SexAI
SfiI SfoI SrfI SwaI