Restriction map for
pFN21AE1683
(Circular) (Six-base) MAPSORT of: pFN21AE1683.seq Check: 7957 from: 1 to: 5293
pFN21AE1683 5293 bp
With 182 enzymes: SgfI *
August 16, 2010 14:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3103 278
Size: 53 83 186 936 1567 2468
Fragments arranged by size:
2468 1567 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2636 1937
Size: 699 4594
Acc65I G'GTAC_C
Cuts at: 2614 2614
Size: 5293
AclI AA'CG_TT
Cuts at: 1563 3420 3793 1563
Size: 1857 373 3063
Fragments arranged by size:
3063 1857 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3356 4740 5008 1632
Size: 1724 1384 268 1917
Fragments arranged by size:
1917 1724 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5096
AflIII A'CryG_T
Cuts at: 1203 4192 5169 1203
Size: 2989 977 1327
Fragments arranged by size:
2989 1327 977
AgeI A'CCGG_T
Cuts at: 4124 4124
Size: 5293
AhdI GACnn_n'nnGTC
Cuts at: 4022 4022
Size: 5293
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5293
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5293
AlwNI CAG_nnn'CTG
Cuts at: 2359 2362 3036 4608 2359
Size: 3 674 1572 3044
Fragments arranged by size:
3044 1572 674 3
ApaLI G'TGCA_C
Cuts at: 3350 4506 3350
Size: 1156 4137
ApoI r'AATT_y
Cuts at: 1493 2602 2828 2980 5089 1493
Size: 1109 226 152 2109 1697
Fragments arranged by size:
2109 1697 1109 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3847 160
Size: 872 2815 1606
Fragments arranged by size:
2815 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5293
AvaI C'yCGr_G
Cuts at: 1870 1943 2098 2618 1870
Size: 73 155 520 4545
Fragments arranged by size:
4545 520 155 73
BamHI G'GATC_C
Cuts at: 2623 2990 2623
Size: 367 4926
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2113 2443 2614 4069 618
Size: 325 217 627 326 330 171 1455 1842
Fragments arranged by size:
1842 1455 627 330 326 325 217 171
BanII G_rGCy'C
Cuts at: 1391 2107 2252 2612 1391
Size: 716 145 360 4072
Fragments arranged by size:
4072 716 360 145
BbeI G_GCGC'C
Cuts at: 2117 2117
Size: 5293
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4322
BbvCI CC'TCA_GC
Cuts at: 2127 2127
Size: 5293
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3484 3518 1903
Size: 34 50 34 1463 34 3678
Fragments arranged by size:
3678 1463 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3185 4395 3185
Size: 1210 4083
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5293
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3904 136
Size: 107 122 71 631 605 2232 1525
Fragments arranged by size:
2232 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5288 5288
Size: 5293
BlpI GC'TnA_GC
Cuts at: 2710 2710
Size: 5293
Bme1580I G_kGCm'C
Cuts at: 1790 3354 4510 1790
Size: 1564 1156 2573
Fragments arranged by size:
2573 1564 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5293
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3024 3982 442
Size: 416 53 83 399 24 1607 958 1753
Fragments arranged by size:
1753 1607 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5293
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3953 1239
Size: 2714 2579
Bpu10I CC'TnA_GC
Cuts at: 2081 2127 2081
Size: 46 5247
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3418 4283 4581 3418
Size: 865 298 4130
Fragments arranged by size:
4130 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3956 882
Size: 617 2457 2219
Fragments arranged by size:
2457 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3084 493
Size: 711 1880 2702
Fragments arranged by size:
2702 1880 711
BsaBI GATnn'nnATC
Cuts at: 5287 5287
Size: 5293
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2114 3100 3482 4117
Size: 53 83 186 936 581 986 382 635
Cuts at: 4117 275
Size: 1451
Fragments arranged by size:
1451 986 936 635 581 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2446 3726 4124 4398 4545 4956 2446
Size: 1280 398 274 147 411 2783
Fragments arranged by size:
2783 1280 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5068
Fragments arranged by size:
5068 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2228 2266 2273 2483 2495 1186
Size: 1042 38 7 210 12 3984
Fragments arranged by size:
3984 1042 210 38 12 7
BseYI C'CCAG_C
Cuts at: 2172 4496 4992 2172
Size: 2324 496 2473
Fragments arranged by size:
2473 2324 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4720
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2192 2256 2328 2743 3504 4532
Size: 275 186 191 64 72 415 761 1028
Cuts at: 4532 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 415 275 191 186 72
64
BsiHKAI G_wGCw'C
Cuts at: 2107 3354 3439 4510 2107
Size: 1247 85 1071 2890
Fragments arranged by size:
2890 1247 1071 85
BsmI GAATG_Cn'
Cuts at: 2425 2804 2897 2425
Size: 379 93 4821
Fragments arranged by size:
4821 379 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2107 2252 2612 3354 3439 4510 1391
Size: 399 317 145 360 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 360 317 145 85
BspEI T'CCGG_A
Cuts at: 4956 4956
Size: 5293
BspHI T'CATG_A
Cuts at: 3182 4912 3182
Size: 1730 3563
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2648 844
Size: 1804 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2258 3180 1394
Size: 429 435 922 3507
Fragments arranged by size:
3507 922 435 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3788 3962 65
Size: 1183 2540 174 1396
Fragments arranged by size:
2540 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2092 2191 2288 3937 4124 1700
Size: 110 282 99 97 1649 187 2869
Fragments arranged by size:
2869 1649 282 187 110 99 97
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5293
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5293
BssSI C'ACGA_G
Cuts at: 3353 4365 3353
Size: 1012 4281
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2359 2362 794
Size: 1565 3 3725
Fragments arranged by size:
3725 1565 3
BstBI TT'CG_AA
Cuts at: 4097 4097
Size: 5293
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5293
BstXI CCAn_nnnn'nTGG
Cuts at: 2485 2485
Size: 5293
BstYI r'GATC_y
Cuts at: 1972 2488 2623 2990 3375 3392 4844 4930 4942
Size: 516 135 367 385 17 1452 86 12
Cuts at: 4942 5288 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 516 385 367 346 135 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2028 2086 2391 513
Size: 552 963 58 305 3415
Fragments arranged by size:
3415 963 552 305 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4554
Fragments arranged by size:
4554 683 56
BtsI GCAGTG_nn'
Cuts at: 2809 3603 3623 2809
Size: 794 20 4479
Fragments arranged by size:
4479 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5293
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5258
DraI TTT'AAA
Cuts at: 2597 2597
Size: 5293
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4300 809
Size: 507 2984 1802
Fragments arranged by size:
2984 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2189 2253 2431
Size: 54 1425 50 275 18 359 64 178
Cuts at: 2431 2526 2740 3629 5258 8
Size: 95 214 889 1629 43
Fragments arranged by size:
1629 1425 889 359 275 214 178 95
64 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2189 2253 2740 1537
Size: 275 377 64 487 4090
Fragments arranged by size:
4090 487 377 275 64
EarI CTCTTCn'nnn_
Cuts at: 1353 3223 1353
Size: 1870 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2216 3880 4254 4400 387
Size: 1064 138 627 1664 374 146 1280
Fragments arranged by size:
1664 1280 1064 627 374 146 138
EcoICRI GAG'CTC
Cuts at: 2105 2105
Size: 5293
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3356 3953 4740 5008 1239
Size: 393 1724 597 787 268 1524
Fragments arranged by size:
1724 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2602 2980 2602
Size: 378 4915
FspI TGC'GCA
Cuts at: 3799 3799
Size: 5293
HaeII r_GCGC'y
Cuts at: 1678 2078 2117 2377 2415 4440 1678
Size: 400 39 260 38 2025 2531
Fragments arranged by size:
2531 2025 400 260 39 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3934 3966 4008 4040 1373
Size: 32 135 32 2362 32 42 32 2626
Fragments arranged by size:
2626 2362 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2637 2883 669
Size: 988 281 699 246 3079
Fragments arranged by size:
3079 988 699 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5293
HpaI GTT'AAC
Cuts at: 2883 2883
Size: 5293
KasI G'GCGC_C
Cuts at: 2113 2113
Size: 5293
KpnI G_GTAC'C
Cuts at: 2618 2618
Size: 5293
MfeI C'AATT_G
Cuts at: 2892 2892
Size: 5293
MluI A'CGCG_T
Cuts at: 5169 5169
Size: 5293
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4407 4591 1305
Size: 3102 184 2007
Fragments arranged by size:
3102 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2528 10
Size: 54 1425 343 696 2775
Fragments arranged by size:
2775 1425 696 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3251 3610 3769 518
Size: 648 2085 359 159 2042
Fragments arranged by size:
2085 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2017 2030 2088 2298 2359 2393 2709 3386
Size: 411 13 58 210 61 34 316 677
Cuts at: 3386 4534 4779 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 411 316 245 210 61
58 34 13
NaeI GCC'GGC
Cuts at: 2290 2290
Size: 5293
NarI GG'CG_CC
Cuts at: 2114 2114
Size: 5293
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4741
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5293
NgoMIV G'CCGG_C
Cuts at: 2288 2288
Size: 5293
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5293
NotI GC'GGCC_GC
Cuts at: 2740 2740
Size: 5293
NspI r_CATG'y
Cuts at: 1132 1229 2651 4196 1132
Size: 97 1422 1545 2229
Fragments arranged by size:
2229 1545 1422 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5293
PciI A'CATG_T
Cuts at: 4192 4192
Size: 5293
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5293
PfoI T'CCnGG_A
Cuts at: 2508 2508
Size: 5293
PmeI GTTT'AAAC
Cuts at: 2597 2597
Size: 5293
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3362 3394 4875 4907 3362
Size: 32 1481 32 3748
Fragments arranged by size:
3748 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5293
PsiI TTA'TAA
Cuts at: 2863 2863
Size: 5293
PstI C_TGCA'G
Cuts at: 830 2355 2645 830
Size: 1525 290 3478
Fragments arranged by size:
3478 1525 290
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5293
PvuII CAG'CTG
Cuts at: 2359 2359
Size: 5293
RsrII CG'GwC_CG
Cuts at: 2463 2463
Size: 5293
SacI G_AGCT'C
Cuts at: 2107 2107
Size: 5293
SacII CC_GC'GG
Cuts at: 2031 2089 2394 2031
Size: 58 305 4930
Fragments arranged by size:
4930 305 58
SalI G'TCGA_C
Cuts at: 1936 2635 1936
Size: 699 4594
SbfI CC_TGCA'GG
Cuts at: 2645 2645
Size: 5293
ScaI AGT'ACT
Cuts at: 2219 3541 5253 2219
Size: 1322 1712 2259
Fragments arranged by size:
2259 1712 1322
SfcI C'TryA_G
Cuts at: 826 1045 2351 2641 3776 4457 4648 826
Size: 219 1306 290 1135 681 191 1471
Fragments arranged by size:
1471 1306 1135 681 290 219 191
SfoI GGC'GCC
Cuts at: 2115 2115
Size: 5293
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5293
SgrAI Cr'CCGG_yG
Cuts at: 1700 2288 1700
Size: 588 4705
SmaI CCC'GGG
Cuts at: 1872 2100 2620 1872
Size: 228 520 4545
Fragments arranged by size:
4545 520 228
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3397 4298 4560 820
Size: 197 926 1454 901 262 1553
Fragments arranged by size:
1553 1454 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3084 493
Size: 2591 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5293
SphI G_CATG'C
Cuts at: 1132 2651 1132
Size: 1519 3774
SspI AAT'ATT
Cuts at: 5 52 3217 5
Size: 47 3165 2081
Fragments arranged by size:
3165 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5293
StyI C'CwwG_G
Cuts at: 513 1065 2133 2732 513
Size: 552 1068 599 3074
Fragments arranged by size:
3074 1068 599 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3320 3337 3490 3675 4120 325
Size: 1255 1740 17 153 185 445 1498
Fragments arranged by size:
1740 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2217 3539 5251
Size: 274 80 33 51 1443 240 1322 1712
Cuts at: 5251 96
Size: 138
Fragments arranged by size:
1712 1443 1322 274 240 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5253 5285 5253
Size: 32 5261
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5293
XbaI T'CTAG_A
Cuts at: 2629 2629
Size: 5293
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5293
XmaI C'CCGG_G
Cuts at: 1870 2098 2618 1870
Size: 228 520 4545
Fragments arranged by size:
4545 520 228
XmnI GAAnn'nnTTC
Cuts at: 3422 3422
Size: 5293
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3101 276
Size: 53 83 186 936 1567 2468
Fragments arranged by size:
2468 1567 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I
EcoRV FalI FseI FspAI NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI SanDI SapI SexAI SfiI
SrfI SwaI XcmI