Restriction map for
pFN21AE1678
(Circular) (Six-base) MAPSORT of: pFN21AE1678.seq Check: 3119 from: 1 to: 5284
pFN21AE1678 5284 bp
With 182 enzymes: SgfI *
August 16, 2010 14:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3094 278
Size: 53 83 186 936 1558 2468
Fragments arranged by size:
2468 1558 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2627 1937
Size: 690 4594
Acc65I G'GTAC_C
Cuts at: 2605 2605
Size: 5284
AclI AA'CG_TT
Cuts at: 1563 3411 3784 1563
Size: 1848 373 3063
Fragments arranged by size:
3063 1848 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3347 4731 4999 1632
Size: 1715 1384 268 1917
Fragments arranged by size:
1917 1715 1384 268
AfeI AGC'GCT
Cuts at: 2123 2123
Size: 5284
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5087
AflIII A'CryG_T
Cuts at: 1203 4183 5160 1203
Size: 2980 977 1327
Fragments arranged by size:
2980 1327 977
AgeI A'CCGG_T
Cuts at: 4115 4115
Size: 5284
AhdI GACnn_n'nnGTC
Cuts at: 4013 4013
Size: 5284
AleI CACnn'nnGTG
Cuts at: 1166 2164 1166
Size: 998 4286
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2444 1184
Size: 1260 4024
AlwNI CAG_nnn'CTG
Cuts at: 2146 2476 3027 4599 2146
Size: 330 551 1572 2831
Fragments arranged by size:
2831 1572 551 330
ApaI G_GGCC'C
Cuts at: 2240 2240
Size: 5284
ApaLI G'TGCA_C
Cuts at: 3341 4497 3341
Size: 1156 4128
ApoI r'AATT_y
Cuts at: 1493 2593 2819 2971 5080 1493
Size: 1100 226 152 2109 1697
Fragments arranged by size:
2109 1697 1100 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3838 160
Size: 872 2806 1606
Fragments arranged by size:
2806 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5284
AvaI C'yCGr_G
Cuts at: 1870 1943 2153 2490 2609 1870
Size: 73 210 337 119 4545
Fragments arranged by size:
4545 337 210 119 73
BamHI G'GATC_C
Cuts at: 2614 2981 2614
Size: 367 4917
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2370 2605 4060 618
Size: 325 217 627 583 235 1455 1842
Fragments arranged by size:
1842 1455 627 583 325 235 217
BanII G_rGCy'C
Cuts at: 1391 2240 2603 1391
Size: 849 363 4072
Fragments arranged by size:
4072 849 363
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4313
BbvCI CC'TCA_GC
Cuts at: 2205 2205
Size: 5284
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3475 3509 1903
Size: 34 50 34 1454 34 3678
Fragments arranged by size:
3678 1454 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2306 3176 4386 2306
Size: 870 1210 3204
Fragments arranged by size:
3204 1210 870
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5284
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3895 136
Size: 107 122 71 631 605 2223 1525
Fragments arranged by size:
2223 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5279 5279
Size: 5284
BlpI GC'TnA_GC
Cuts at: 2136 2178 2701 2136
Size: 42 523 4719
Fragments arranged by size:
4719 523 42
Bme1580I G_kGCm'C
Cuts at: 1790 2240 3345 4501 1790
Size: 450 1105 1156 2573
Fragments arranged by size:
2573 1156 1105 450
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5284
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2255 2529 3015
Size: 416 53 83 399 24 838 274 486
Cuts at: 3015 3973 442
Size: 958 1753
Fragments arranged by size:
1753 958 838 486 416 399 274 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5284
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2350 3944 1239
Size: 1111 1594 2579
Fragments arranged by size:
2579 1594 1111
Bpu10I CC'TnA_GC
Cuts at: 2205 2205
Size: 5284
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3409 4274 4572 3409
Size: 865 298 4121
Fragments arranged by size:
4121 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2546 3947 882
Size: 617 1047 1401 2219
Fragments arranged by size:
2219 1401 1047 617
BsaAI yAC'GTr
Cuts at: 493 1204 2294 3075 493
Size: 711 1090 781 2702
Fragments arranged by size:
2702 1090 781 711
BsaBI GATnn'nnATC
Cuts at: 5278 5278
Size: 5284
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2480 3091 3473 4108
Size: 53 83 186 936 947 611 382 635
Cuts at: 4108 275
Size: 1451
Fragments arranged by size:
1451 947 936 635 611 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3717 4115 4389 4536 4947 3717
Size: 398 274 147 411 4054
Fragments arranged by size:
4054 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5059
Fragments arranged by size:
5059 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2341 1186
Size: 841 314 4129
Fragments arranged by size:
4129 841 314
BseYI C'CCAG_C
Cuts at: 2239 2469 4487 4983 2239
Size: 230 2018 496 2540
Fragments arranged by size:
2540 2018 496 230
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2491 2588 1163
Size: 573 755 97 3859
Fragments arranged by size:
3859 755 573 97
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2734 3495 4523 1540
Size: 275 186 733 761 1028 2301
Fragments arranged by size:
2301 1028 761 733 275 186
BsiHKAI G_wGCw'C
Cuts at: 3345 3430 4501 3345
Size: 85 1071 4128
Fragments arranged by size:
4128 1071 85
BsmI GAATG_Cn'
Cuts at: 2795 2888 2795
Size: 93 5191
BsmBI CGTCTCn'nnnn_
Cuts at: 2441 2441
Size: 5284
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2240 2603 3345 3430 4501 1391
Size: 399 450 363 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 450 399 363 85
BspEI T'CCGG_A
Cuts at: 4947 4947
Size: 5284
BspHI T'CATG_A
Cuts at: 3173 4903 3173
Size: 1730 3554
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2639 844
Size: 1795 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2152 2253 3171 1394
Size: 429 329 101 918 3507
Fragments arranged by size:
3507 918 429 329 101
BsrDI GCAATG_nn'
Cuts at: 65 1248 2460 3779 3953 65
Size: 1183 1212 1319 174 1396
Fragments arranged by size:
1396 1319 1212 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3928 4115 1700
Size: 110 2118 187 2869
Fragments arranged by size:
2869 2118 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5284
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5284
BssSI C'ACGA_G
Cuts at: 2105 3344 4356 2105
Size: 1239 1012 3033
Fragments arranged by size:
3033 1239 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2146 2575 794
Size: 1352 429 3503
Fragments arranged by size:
3503 1352 429
BstBI TT'CG_AA
Cuts at: 4088 4088
Size: 5284
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5284
BstXI CCAn_nnnn'nTGG
Cuts at: 2306 2386 2306
Size: 80 5204
BstYI r'GATC_y
Cuts at: 1972 2614 2981 3366 3383 4835 4921 4933 5279
Size: 642 367 385 17 1452 86 12 346
Cuts at: 5279 1972
Size: 1977
Fragments arranged by size:
1977 1452 642 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2263 2391 513
Size: 552 1198 128 3406
Fragments arranged by size:
3406 1198 552 128
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4545
Fragments arranged by size:
4545 683 56
BtsI GCAGTG_nn'
Cuts at: 2576 2800 3594 3614 2576
Size: 224 794 20 4246
Fragments arranged by size:
4246 794 224 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5284
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2435 2470 529
Size: 35 1871 35 3343
Fragments arranged by size:
3343 1871 35 35
DraI TTT'AAA
Cuts at: 2588 2588
Size: 5284
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4291 809
Size: 507 2975 1802
Fragments arranged by size:
2975 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2340 2388 2731
Size: 54 1425 50 275 18 510 48 343
Cuts at: 2731 3620 5249 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 510 343 275 54 50
48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2731 1537
Size: 275 919 4090
Fragments arranged by size:
4090 919 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3214 1353
Size: 1861 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3871 4245 4391 387
Size: 1064 138 2282 374 146 1280
Fragments arranged by size:
2282 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2350 3347 3944 4731 4999 1239
Size: 393 718 997 597 787 268 1524
Fragments arranged by size:
1524 997 787 718 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2043 2201 2212 2236 2043
Size: 158 11 24 5091
Fragments arranged by size:
5091 158 24 11
EcoRI G'AATT_C
Cuts at: 2593 2971 2593
Size: 378 4906
FspI TGC'GCA
Cuts at: 3790 3790
Size: 5284
HaeII r_GCGC'y
Cuts at: 1678 2125 2476 4431 1678
Size: 447 351 1955 2531
Fragments arranged by size:
2531 1955 447 351
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3925 3957 3999 4031 1373
Size: 32 135 32 2353 32 42 32 2626
Fragments arranged by size:
2626 2353 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2628 2874 669
Size: 988 281 690 246 3079
Fragments arranged by size:
3079 988 690 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5284
HpaI GTT'AAC
Cuts at: 2874 2874
Size: 5284
KpnI G_GTAC'C
Cuts at: 2609 2609
Size: 5284
MfeI C'AATT_G
Cuts at: 2883 2883
Size: 5284
MluI A'CGCG_T
Cuts at: 5160 5160
Size: 5284
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4398 4582 1305
Size: 3093 184 2007
Fragments arranged by size:
3093 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2342 10
Size: 54 1425 343 510 2952
Fragments arranged by size:
2952 1425 510 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2164 2304 2315 2330 3242 3601 3760
Size: 648 998 140 11 15 912 359 159
Cuts at: 3760 518
Size: 2042
Fragments arranged by size:
2042 998 912 648 359 159 140 15
11
MspA1I CmG'CkG
Cuts at: 1606 2146 2188 2700 3377 4525 4770 1606
Size: 540 42 512 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 540 512 245 42
NcoI C'CATG_G
Cuts at: 513 1065 2263 513
Size: 552 1198 3534
Fragments arranged by size:
3534 1198 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5284
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5284
NotI GC'GGCC_GC
Cuts at: 2731 2731
Size: 5284
NspI r_CATG'y
Cuts at: 1132 1229 2642 4187 1132
Size: 97 1413 1545 2229
Fragments arranged by size:
2229 1545 1413 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5284
PciI A'CATG_T
Cuts at: 4183 4183
Size: 5284
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5284
PmeI GTTT'AAAC
Cuts at: 2588 2588
Size: 5284
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3353 3385 4866 4898 3353
Size: 32 1481 32 3739
Fragments arranged by size:
3739 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2201 2212 2201
Size: 11 5273
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5284
PsiI TTA'TAA
Cuts at: 2854 2854
Size: 5284
PspOMI G'GGCC_C
Cuts at: 2236 2236
Size: 5284
PspXI vC'TCGA_Gb
Cuts at: 2153 2153
Size: 5284
PstI C_TGCA'G
Cuts at: 830 2187 2636 830
Size: 1357 449 3478
Fragments arranged by size:
3478 1357 449
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5284
PvuII CAG'CTG
Cuts at: 2146 2188 2146
Size: 42 5242
SalI G'TCGA_C
Cuts at: 1936 2626 1936
Size: 690 4594
SbfI CC_TGCA'GG
Cuts at: 2636 2636
Size: 5284
ScaI AGT'ACT
Cuts at: 3532 5244 3532
Size: 1712 3572
SfcI C'TryA_G
Cuts at: 826 1045 2183 2632 3767 4448 4639 826
Size: 219 1138 449 1135 681 191 1471
Fragments arranged by size:
1471 1138 1135 681 449 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5284
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5284
SmaI CCC'GGG
Cuts at: 1872 2611 1872
Size: 739 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2153 3388 4289 4551 820
Size: 197 926 210 1235 901 262 1553
Fragments arranged by size:
1553 1235 926 901 262 210 197
SnaBI TAC'GTA
Cuts at: 493 2294 3075 493
Size: 1801 781 2702
Fragments arranged by size:
2702 1801 781
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5284
SphI G_CATG'C
Cuts at: 1132 2642 1132
Size: 1510 3774
SspI AAT'ATT
Cuts at: 5 52 3208 5
Size: 47 3156 2081
Fragments arranged by size:
3156 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5284
StyI C'CwwG_G
Cuts at: 513 1065 2263 2449 2723 513
Size: 552 1198 186 274 3074
Fragments arranged by size:
3074 1198 552 274 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2040 2154 2563 3311 3328 3481 3666
Size: 1255 460 114 409 748 17 153 185
Cuts at: 3666 4111 325
Size: 445 1498
Fragments arranged by size:
1498 1255 748 460 445 409 185 153
114 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3530 5242 96
Size: 274 80 33 51 1443 1553 1712 138
Fragments arranged by size:
1712 1553 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5244 5276 5244
Size: 32 5252
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5284
XbaI T'CTAG_A
Cuts at: 2620 2620
Size: 5284
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2389 2389
Size: 5284
XhoI C'TCGA_G
Cuts at: 1943 2153 1943
Size: 210 5074
XmaI C'CCGG_G
Cuts at: 1870 2609 1870
Size: 739 4545
XmnI GAAnn'nnTTC
Cuts at: 3413 3413
Size: 5284
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3092 276
Size: 53 83 186 936 1558 2468
Fragments arranged by size:
2468 1558 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI