Restriction map for
pFN21AE1669
(Circular) (Six-base) MAPSORT of: pFN21AE1669.seq Check: 1041 from: 1 to: 5269
pFN21AE1669 5269 bp
With 182 enzymes: SgfI *
August 16, 2010 14:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3079 278
Size: 53 83 186 936 1543 2468
Fragments arranged by size:
2468 1543 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2612 1937
Size: 675 4594
Acc65I G'GTAC_C
Cuts at: 2590 2590
Size: 5269
AclI AA'CG_TT
Cuts at: 1563 3396 3769 1563
Size: 1833 373 3063
Fragments arranged by size:
3063 1833 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2125 3332 4716 4984 1632
Size: 493 1207 1384 268 1917
Fragments arranged by size:
1917 1384 1207 493 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5072
AflIII A'CryG_T
Cuts at: 1203 4168 5145 1203
Size: 2965 977 1327
Fragments arranged by size:
2965 1327 977
AgeI A'CCGG_T
Cuts at: 4100 4100
Size: 5269
AhdI GACnn_n'nnGTC
Cuts at: 3998 3998
Size: 5269
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5269
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5269
AlwNI CAG_nnn'CTG
Cuts at: 2247 3012 4584 2247
Size: 765 1572 2932
Fragments arranged by size:
2932 1572 765
ApaLI G'TGCA_C
Cuts at: 3326 4482 3326
Size: 1156 4113
ApoI r'AATT_y
Cuts at: 1493 2108 2578 2804 2956 5065 1493
Size: 615 470 226 152 2109 1697
Fragments arranged by size:
2109 1697 615 470 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3823 160
Size: 872 2791 1606
Fragments arranged by size:
2791 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5269
AvaI C'yCGr_G
Cuts at: 1870 1943 2124 2594 1870
Size: 73 181 470 4545
Fragments arranged by size:
4545 470 181 73
BamHI G'GATC_C
Cuts at: 2599 2966 2599
Size: 367 4902
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2590 4045 618
Size: 325 217 627 803 1455 1842
Fragments arranged by size:
1842 1455 803 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2588 1391
Size: 1197 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2184 928
Size: 971 285 4013
Fragments arranged by size:
4013 971 285
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3460 3494 1903
Size: 34 1523 34 3678
Fragments arranged by size:
3678 1523 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2123 2211 3161 4371 2123
Size: 88 950 1210 3021
Fragments arranged by size:
3021 1210 950 88
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5269
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3880 136
Size: 107 122 71 631 605 2208 1525
Fragments arranged by size:
2208 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5264 5264
Size: 5269
BlpI GC'TnA_GC
Cuts at: 2686 2686
Size: 5269
Bme1580I G_kGCm'C
Cuts at: 1790 2361 3330 4486 1790
Size: 571 969 1156 2573
Fragments arranged by size:
2573 1156 969 571
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5269
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2087 3000 3958
Size: 416 53 83 399 24 670 913 958
Cuts at: 3958 442
Size: 1753
Fragments arranged by size:
1753 958 913 670 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5269
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2478 3929 1239
Size: 1239 1451 2579
Fragments arranged by size:
2579 1451 1239
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3394 4259 4557 3394
Size: 865 298 4106
Fragments arranged by size:
4106 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3932 882
Size: 617 2433 2219
Fragments arranged by size:
2433 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2516 3060 493
Size: 711 1312 544 2702
Fragments arranged by size:
2702 1312 711 544
BsaBI GATnn'nnATC
Cuts at: 2048 5263 2048
Size: 3215 2054
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3076 3458 4093 275
Size: 53 83 186 936 1543 382 635 1451
Fragments arranged by size:
1543 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3702 4100 4374 4521 4932 3702
Size: 398 274 147 411 4039
Fragments arranged by size:
4039 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5044
Fragments arranged by size:
5044 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2183 1186
Size: 997 4272
BseYI C'CCAG_C
Cuts at: 2095 2196 2275 4472 4968 2095
Size: 101 79 2197 496 2396
Fragments arranged by size:
2396 2197 496 101 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2496 1163
Size: 573 760 3936
Fragments arranged by size:
3936 760 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2719 3480 4508 1540
Size: 275 186 718 761 1028 2301
Fragments arranged by size:
2301 1028 761 718 275 186
BsiHKAI G_wGCw'C
Cuts at: 2328 2475 3330 3415 4486 2328
Size: 147 855 85 1071 3111
Fragments arranged by size:
3111 1071 855 147 85
BsmI GAATG_Cn'
Cuts at: 2780 2873 2780
Size: 93 5176
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2328 2361 2475 2588 3330 3415 4486
Size: 399 538 33 114 113 742 85 1071
Cuts at: 4486 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 538 399 114 113 85
33
BspEI T'CCGG_A
Cuts at: 4932 4932
Size: 5269
BspHI T'CATG_A
Cuts at: 3158 4888 3158
Size: 1730 3539
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2255 2276 2624 844
Size: 1411 21 348 3489
Fragments arranged by size:
3489 1411 348 21
BsrBI CCG'CTC
Cuts at: 1394 1823 3156 1394
Size: 429 1333 3507
Fragments arranged by size:
3507 1333 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3764 3938 65
Size: 1183 2516 174 1396
Fragments arranged by size:
2516 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3913 4100 1700
Size: 110 2103 187 2869
Fragments arranged by size:
2869 2103 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5269
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5269
BssSI C'ACGA_G
Cuts at: 2477 3329 4341 2477
Size: 852 1012 3405
Fragments arranged by size:
3405 1012 852
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2332 794
Size: 1538 3731
BstBI TT'CG_AA
Cuts at: 4073 4073
Size: 5269
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5269
BstYI r'GATC_y
Cuts at: 1972 2599 2966 3351 3368 4820 4906 4918 5264
Size: 627 367 385 17 1452 86 12 346
Cuts at: 5264 1972
Size: 1977
Fragments arranged by size:
1977 1452 627 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2430 2430
Size: 5269
BtgI C'CryG_G
Cuts at: 513 1065 2004 2211 513
Size: 552 939 207 3571
Fragments arranged by size:
3571 939 552 207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4530
Fragments arranged by size:
4530 683 56
BtsI GCAGTG_nn'
Cuts at: 2785 3579 3599 2785
Size: 794 20 4455
Fragments arranged by size:
4455 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5269
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5234
DraI TTT'AAA
Cuts at: 2573 2573
Size: 5269
DraIII CAC_nnn'GTG
Cuts at: 2332 2479 2332
Size: 147 5122
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4276 809
Size: 507 2960 1802
Fragments arranged by size:
2960 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2716 3605
Size: 54 1425 50 275 18 225 661 889
Cuts at: 3605 5234 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 661 275 225 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2716 1537
Size: 275 904 4090
Fragments arranged by size:
4090 904 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3199 1353
Size: 1846 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3856 4230 4376 387
Size: 1064 138 2267 374 146 1280
Fragments arranged by size:
2267 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2125 2478 3332 3929 4716 4984 1239
Size: 393 493 353 854 597 787 268 1524
Fragments arranged by size:
1524 854 787 597 493 393 353 268
EcoO109I rG'GnC_Cy
Cuts at: 2280 2280
Size: 5269
EcoRI G'AATT_C
Cuts at: 2578 2956 2578
Size: 378 4891
FspI TGC'GCA
Cuts at: 2336 3775 2336
Size: 1439 3830
HaeII r_GCGC'y
Cuts at: 1678 4416 1678
Size: 2738 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3910 3942 3984 4016 1373
Size: 32 135 32 2338 32 42 32 2626
Fragments arranged by size:
2626 2338 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2613 2859 669
Size: 988 281 675 246 3079
Fragments arranged by size:
3079 988 675 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5269
HpaI GTT'AAC
Cuts at: 2859 2859
Size: 5269
KpnI G_GTAC'C
Cuts at: 2594 2594
Size: 5269
MfeI C'AATT_G
Cuts at: 2868 2868
Size: 5269
MluI A'CGCG_T
Cuts at: 5145 5145
Size: 5269
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4383 4567 1305
Size: 3078 184 2007
Fragments arranged by size:
3078 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2057 10
Size: 54 1425 343 225 3222
Fragments arranged by size:
3222 1425 343 225 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2528 3227 3586 3745 518
Size: 648 1362 699 359 159 2042
Fragments arranged by size:
2042 1362 699 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2421 2685 3362 4510 4755 1606
Size: 815 264 677 1148 245 2120
Fragments arranged by size:
2120 1148 815 677 264 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2211 513
Size: 552 939 207 3571
Fragments arranged by size:
3571 939 552 207
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5269
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5269
NotI GC'GGCC_GC
Cuts at: 2716 2716
Size: 5269
NspI r_CATG'y
Cuts at: 1132 1229 2275 2627 4172 1132
Size: 97 1046 352 1545 2229
Fragments arranged by size:
2229 1545 1046 352 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5269
PciI A'CATG_T
Cuts at: 4168 4168
Size: 5269
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5269
PfoI T'CCnGG_A
Cuts at: 2486 2486
Size: 5269
PmeI GTTT'AAAC
Cuts at: 2573 2573
Size: 5269
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3338 3370 4851 4883 3338
Size: 32 1481 32 3724
Fragments arranged by size:
3724 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2280 2280
Size: 5269
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5269
PsiI TTA'TAA
Cuts at: 2839 2839
Size: 5269
PstI C_TGCA'G
Cuts at: 830 2015 2621 830
Size: 1185 606 3478
Fragments arranged by size:
3478 1185 606
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5269
SalI G'TCGA_C
Cuts at: 1936 2611 1936
Size: 675 4594
SbfI CC_TGCA'GG
Cuts at: 2621 2621
Size: 5269
ScaI AGT'ACT
Cuts at: 3517 5229 3517
Size: 1712 3557
SfcI C'TryA_G
Cuts at: 826 1045 2011 2617 3752 4433 4624 826
Size: 219 966 606 1135 681 191 1471
Fragments arranged by size:
1471 1135 966 681 606 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5269
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5269
SmaI CCC'GGG
Cuts at: 1872 2596 1872
Size: 724 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3373 4274 4536 820
Size: 197 926 1430 901 262 1553
Fragments arranged by size:
1553 1430 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3060 493
Size: 2567 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5269
SphI G_CATG'C
Cuts at: 1132 2275 2627 1132
Size: 1143 352 3774
Fragments arranged by size:
3774 1143 352
SspI AAT'ATT
Cuts at: 5 52 3193 5
Size: 47 3141 2081
Fragments arranged by size:
3141 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5269
StyI C'CwwG_G
Cuts at: 513 1065 2004 2211 2708 513
Size: 552 939 207 497 3074
Fragments arranged by size:
3074 939 552 497 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2178 3296 3313 3466 3651 4096 325
Size: 1255 598 1118 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1118 598 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3515 5227 96
Size: 274 80 33 51 1443 1538 1712 138
Fragments arranged by size:
1712 1538 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5229 5261 5229
Size: 32 5237
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5269
XbaI T'CTAG_A
Cuts at: 2605 2605
Size: 5269
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5269
XmaI C'CCGG_G
Cuts at: 1870 2594 1870
Size: 724 4545
XmnI GAAnn'nnTTC
Cuts at: 3398 3398
Size: 5269
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3077 276
Size: 53 83 186 936 1543 2468
Fragments arranged by size:
2468 1543 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI