Restriction map for
pFN21AE1665
(Circular) (Six-base) MAPSORT of: pFN21AE1665.seq Check: 8770 from: 1 to: 5257
pFN21AE1665 5257 bp
With 182 enzymes: SgfI *
August 16, 2010 14:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3067 278
Size: 53 83 186 936 1531 2468
Fragments arranged by size:
2468 1531 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2600 1937
Size: 663 4594
Acc65I G'GTAC_C
Cuts at: 2578 2578
Size: 5257
AclI AA'CG_TT
Cuts at: 1563 3384 3757 1563
Size: 1821 373 3063
Fragments arranged by size:
3063 1821 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3320 4704 4972 1632
Size: 1688 1384 268 1917
Fragments arranged by size:
1917 1688 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5060
AflIII A'CryG_T
Cuts at: 1203 2554 4156 5133 1203
Size: 1351 1602 977 1327
Fragments arranged by size:
1602 1351 1327 977
AgeI A'CCGG_T
Cuts at: 4088 4088
Size: 5257
AhdI GACnn_n'nnGTC
Cuts at: 3986 3986
Size: 5257
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5257
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5257
AlwNI CAG_nnn'CTG
Cuts at: 3000 4572 3000
Size: 1572 3685
ApaLI G'TGCA_C
Cuts at: 3314 4470 3314
Size: 1156 4101
ApoI r'AATT_y
Cuts at: 1493 2396 2566 2792 2944 5053 1493
Size: 903 170 226 152 2109 1697
Fragments arranged by size:
2109 1697 903 226 170 152
AseI AT'TA_AT
Cuts at: 160 1032 3811 160
Size: 872 2779 1606
Fragments arranged by size:
2779 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5257
AvaI C'yCGr_G
Cuts at: 1870 1943 2239 2582 1870
Size: 73 296 343 4545
Fragments arranged by size:
4545 343 296 73
BamHI G'GATC_C
Cuts at: 2272 2587 2954 2272
Size: 315 367 4575
Fragments arranged by size:
4575 367 315
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2318 2578 4033 618
Size: 325 217 627 531 260 1455 1842
Fragments arranged by size:
1842 1455 627 531 325 260 217
BanII G_rGCy'C
Cuts at: 1391 2576 1391
Size: 1185 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2421 928
Size: 971 522 3764
Fragments arranged by size:
3764 971 522
BbvCI CC'TCA_GC
Cuts at: 2246 2246
Size: 5257
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3448 3482 1903
Size: 34 1511 34 3678
Fragments arranged by size:
3678 1511 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3149 4359 3149
Size: 1210 4047
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5257
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2361 3868 136
Size: 107 122 71 631 605 689 1507 1525
Fragments arranged by size:
1525 1507 689 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5252 5252
Size: 5257
BlpI GC'TnA_GC
Cuts at: 2674 2674
Size: 5257
Bme1580I G_kGCm'C
Cuts at: 1790 3318 4474 1790
Size: 1528 1156 2573
Fragments arranged by size:
2573 1528 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5257
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2988 3946 442
Size: 416 53 83 399 24 1571 958 1753
Fragments arranged by size:
1753 1571 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5257
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2083 2493 3917 1239
Size: 844 410 1424 2579
Fragments arranged by size:
2579 1424 844 410
Bpu10I CC'TnA_GC
Cuts at: 2246 2246
Size: 5257
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3382 4247 4545 3382
Size: 865 298 4094
Fragments arranged by size:
4094 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2336 2529 3920 882
Size: 617 837 193 1391 2219
Fragments arranged by size:
2219 1391 837 617 193
BsaAI yAC'GTr
Cuts at: 493 1204 3048 493
Size: 711 1844 2702
Fragments arranged by size:
2702 1844 711
BsaBI GATnn'nnATC
Cuts at: 5251 5251
Size: 5257
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3064 3446 4081 275
Size: 53 83 186 936 1531 382 635 1451
Fragments arranged by size:
1531 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3690 4088 4362 4509 4920 3690
Size: 398 274 147 411 4027
Fragments arranged by size:
4027 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5032
Fragments arranged by size:
5032 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 1186
Size: 838 4419
BseYI C'CCAG_C
Cuts at: 4460 4956 4460
Size: 496 4761
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2028 1163
Size: 573 292 4392
Fragments arranged by size:
4392 573 292
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2707 3468 4496 1540
Size: 275 186 706 761 1028 2301
Fragments arranged by size:
2301 1028 761 706 275 186
BsiHKAI G_wGCw'C
Cuts at: 2148 3318 3403 4474 2148
Size: 1170 85 1071 2931
Fragments arranged by size:
2931 1170 1071 85
BsmI GAATG_Cn'
Cuts at: 2518 2768 2861 2518
Size: 250 93 4914
Fragments arranged by size:
4914 250 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2148 2576 3318 3403 4474 1391
Size: 399 358 428 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 428 399 358 85
BspEI T'CCGG_A
Cuts at: 4920 4920
Size: 5257
BspHI T'CATG_A
Cuts at: 3146 4876 3146
Size: 1730 3527
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2354 2612 844
Size: 1510 258 3489
Fragments arranged by size:
3489 1510 258
BsrBI CCG'CTC
Cuts at: 1394 1823 3144 1394
Size: 429 1321 3507
Fragments arranged by size:
3507 1321 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3752 3926 65
Size: 1183 2504 174 1396
Fragments arranged by size:
2504 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3901 4088 1700
Size: 110 2091 187 2869
Fragments arranged by size:
2869 2091 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5257
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5257
BssSI C'ACGA_G
Cuts at: 2036 3317 4329 2036
Size: 1281 1012 2964
Fragments arranged by size:
2964 1281 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2112 794
Size: 1318 3939
BstBI TT'CG_AA
Cuts at: 4061 4061
Size: 5257
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5257
BstYI r'GATC_y
Cuts at: 1972 2272 2587 2954 3339 3356 4808 4894 4906
Size: 300 315 367 385 17 1452 86 12
Cuts at: 4906 5252 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 315 300 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4705
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4518
Fragments arranged by size:
4518 683 56
BtsI GCAGTG_nn'
Cuts at: 2113 2773 3567 3587 2113
Size: 660 794 20 3783
Fragments arranged by size:
3783 794 660 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5257
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5222
DraI TTT'AAA
Cuts at: 2561 2561
Size: 5257
DraIII CAC_nnn'GTG
Cuts at: 2053 2053
Size: 5257
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4264 809
Size: 507 2948 1802
Fragments arranged by size:
2948 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2704 3593
Size: 54 1425 50 275 18 225 649 889
Cuts at: 3593 5222 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 649 275 225 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2704 1537
Size: 275 892 4090
Fragments arranged by size:
4090 892 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2003 2280 3187 1353
Size: 650 277 907 3423
Fragments arranged by size:
3423 907 650 277
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3844 4218 4364 387
Size: 1064 138 2255 374 146 1280
Fragments arranged by size:
2255 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2083 2493 3320 3917 4704 4972 1239
Size: 393 451 410 827 597 787 268 1524
Fragments arranged by size:
1524 827 787 597 451 410 393 268
EcoRI G'AATT_C
Cuts at: 2566 2944 2566
Size: 378 4879
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2032 2032
Size: 5257
FspI TGC'GCA
Cuts at: 3763 3763
Size: 5257
HaeII r_GCGC'y
Cuts at: 1678 4404 1678
Size: 2726 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2453 2485 3898 3930 3972
Size: 32 135 32 881 32 1413 32 42
Cuts at: 3972 4004 1373
Size: 32 2626
Fragments arranged by size:
2626 1413 881 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2601 2847 669
Size: 988 281 663 246 3079
Fragments arranged by size:
3079 988 663 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5257
HpaI GTT'AAC
Cuts at: 2847 2847
Size: 5257
KpnI G_GTAC'C
Cuts at: 2582 2582
Size: 5257
MfeI C'AATT_G
Cuts at: 2856 2856
Size: 5257
MluI A'CGCG_T
Cuts at: 2554 5133 2554
Size: 2579 2678
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2457 4371 4555 1305
Size: 1152 1914 184 2007
Fragments arranged by size:
2007 1914 1152 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3435
Fragments arranged by size:
3435 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3215 3574 3733 518
Size: 648 2049 359 159 2042
Fragments arranged by size:
2049 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2673 3350 4498 4743 1606
Size: 1067 677 1148 245 2120
Fragments arranged by size:
2120 1148 1067 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4705
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5257
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5257
NotI GC'GGCC_GC
Cuts at: 2704 2704
Size: 5257
NspI r_CATG'y
Cuts at: 1132 1229 2615 4160 1132
Size: 97 1386 1545 2229
Fragments arranged by size:
2229 1545 1386 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5257
PciI A'CATG_T
Cuts at: 4156 4156
Size: 5257
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5257
PmeI GTTT'AAAC
Cuts at: 2561 2561
Size: 5257
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3326 3358 4839 4871 3326
Size: 32 1481 32 3712
Fragments arranged by size:
3712 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5257
PsiI TTA'TAA
Cuts at: 2827 2827
Size: 5257
PstI C_TGCA'G
Cuts at: 830 2182 2609 830
Size: 1352 427 3478
Fragments arranged by size:
3478 1352 427
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5257
SalI G'TCGA_C
Cuts at: 1936 2599 1936
Size: 663 4594
SapI GCTCTTCn'nnn_
Cuts at: 2003 2003
Size: 5257
SbfI CC_TGCA'GG
Cuts at: 2609 2609
Size: 5257
ScaI AGT'ACT
Cuts at: 3505 5217 3505
Size: 1712 3545
SexAI A'CCwGG_T
Cuts at: 2049 2049
Size: 5257
SfcI C'TryA_G
Cuts at: 826 1045 2178 2605 3740 4421 4612 826
Size: 219 1133 427 1135 681 191 1471
Fragments arranged by size:
1471 1135 1133 681 427 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5257
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5257
SmaI CCC'GGG
Cuts at: 1872 2241 2584 1872
Size: 369 343 4545
Fragments arranged by size:
4545 369 343
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3361 4262 4524 820
Size: 197 926 1418 901 262 1553
Fragments arranged by size:
1553 1418 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3048 493
Size: 2555 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5257
SphI G_CATG'C
Cuts at: 1132 2615 1132
Size: 1483 3774
SspI AAT'ATT
Cuts at: 5 52 2414 2485 3181 5
Size: 47 2362 71 696 2081
Fragments arranged by size:
2362 2081 696 71 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5257
StyI C'CwwG_G
Cuts at: 513 1065 2696 513
Size: 552 1631 3074
Fragments arranged by size:
3074 1631 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3284 3301 3454 3639 4084 325
Size: 1255 1704 17 153 185 445 1498
Fragments arranged by size:
1704 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3503 5215 96
Size: 274 80 33 51 1443 1526 1712 138
Fragments arranged by size:
1712 1526 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5217 5249 5217
Size: 32 5225
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5257
XbaI T'CTAG_A
Cuts at: 2593 2593
Size: 5257
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5257
XmaI C'CCGG_G
Cuts at: 1870 2239 2582 1870
Size: 369 343 4545
Fragments arranged by size:
4545 369 343
XmnI GAAnn'nnTTC
Cuts at: 3386 3386
Size: 5257
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3065 276
Size: 53 83 186 936 1531 2468
Fragments arranged by size:
2468 1531 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI
SfoI SrfI SwaI XcmI