Restriction map for
pFN21AE1657
(Circular) (Six-base) MAPSORT of: pFN21AE1657.seq Check: 2365 from: 1 to: 5224
pFN21AE1657 5224 bp
With 182 enzymes: SgfI *
August 16, 2010 14:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2285 2285
Size: 5224
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3034 278
Size: 53 83 186 936 1498 2468
Fragments arranged by size:
2468 1498 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2567 1937
Size: 630 4594
Acc65I G'GTAC_C
Cuts at: 2545 2545
Size: 5224
AclI AA'CG_TT
Cuts at: 1563 3351 3724 1563
Size: 1788 373 3063
Fragments arranged by size:
3063 1788 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3287 4671 4939 1632
Size: 1655 1384 268 1917
Fragments arranged by size:
1917 1655 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5027
AflIII A'CryG_T
Cuts at: 1203 2189 4123 5100 1203
Size: 986 1934 977 1327
Fragments arranged by size:
1934 1327 986 977
AgeI A'CCGG_T
Cuts at: 4055 4055
Size: 5224
AhdI GACnn_n'nnGTC
Cuts at: 3953 3953
Size: 5224
AleI CACnn'nnGTG
Cuts at: 1166 2114 1166
Size: 948 4276
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5224
AlwNI CAG_nnn'CTG
Cuts at: 2967 4539 2967
Size: 1572 3652
ApaI G_GGCC'C
Cuts at: 2174 2174
Size: 5224
ApaLI G'TGCA_C
Cuts at: 3281 4437 3281
Size: 1156 4068
ApoI r'AATT_y
Cuts at: 1493 2423 2533 2759 2911 5020 1493
Size: 930 110 226 152 2109 1697
Fragments arranged by size:
2109 1697 930 226 152 110
AseI AT'TA_AT
Cuts at: 160 1032 3778 160
Size: 872 2746 1606
Fragments arranged by size:
2746 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5224
AvaI C'yCGr_G
Cuts at: 1870 1943 2173 2459 2549 1870
Size: 73 230 286 90 4545
Fragments arranged by size:
4545 286 230 90 73
AvrII C'CTAG_G
Cuts at: 2065 2065
Size: 5224
BamHI G'GATC_C
Cuts at: 2554 2921 2554
Size: 367 4857
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 2545 4000 618
Size: 325 217 627 224 534 1455 1842
Fragments arranged by size:
1842 1455 627 534 325 224 217
BanII G_rGCy'C
Cuts at: 1391 2109 2174 2543 1391
Size: 718 65 369 4072
Fragments arranged by size:
4072 718 369 65
BbeI G_GCGC'C
Cuts at: 2015 2015
Size: 5224
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2265 928
Size: 971 366 3887
Fragments arranged by size:
3887 971 366
BbvCI CC'TCA_GC
Cuts at: 2081 2081
Size: 5224
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3415 3449 1903
Size: 34 1478 34 3678
Fragments arranged by size:
3678 1478 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3116 4326 3116
Size: 1210 4014
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5224
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3835 136
Size: 107 122 71 631 605 337 1826 1525
Fragments arranged by size:
1826 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5219 5219
Size: 5224
BlpI GC'TnA_GC
Cuts at: 2475 2641 2475
Size: 166 5058
Bme1580I G_kGCm'C
Cuts at: 1790 2174 3285 4441 1790
Size: 384 1111 1156 2573
Fragments arranged by size:
2573 1156 1111 384
BmgBI CAC'GTC
Cuts at: 1318 2128 1318
Size: 810 4414
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2955 3913 442
Size: 416 53 83 399 24 1538 958 1753
Fragments arranged by size:
1753 1538 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5224
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2308 3884 1239
Size: 1069 1576 2579
Fragments arranged by size:
2579 1576 1069
Bpu10I CC'TnA_GC
Cuts at: 2081 2081
Size: 5224
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2196 3349 4214 4512 2196
Size: 1153 865 298 2908
Fragments arranged by size:
2908 1153 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3887 882
Size: 617 2388 2219
Fragments arranged by size:
2388 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2190 3015 493
Size: 711 986 825 2702
Fragments arranged by size:
2702 986 825 711
BsaBI GATnn'nnATC
Cuts at: 5218 5218
Size: 5224
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2034 2142 3031
Size: 53 83 186 936 479 22 108 889
Cuts at: 3031 3413 4048 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 889 635 479 382 186 108
83 53 22
BsaWI w'CCGG_w
Cuts at: 3657 4055 4329 4476 4887 3657
Size: 398 274 147 411 3994
Fragments arranged by size:
3994 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2084 2114 1178
Size: 30 165 30 681 30 4288
Fragments arranged by size:
4288 681 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5224
BseYI C'CCAG_C
Cuts at: 2015 4427 4923 2015
Size: 2412 496 2316
Fragments arranged by size:
2412 2316 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2263 1163
Size: 573 527 4124
Fragments arranged by size:
4124 573 527
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2674 3435 4463 1540
Size: 275 186 673 761 1028 2301
Fragments arranged by size:
2301 1028 761 673 275 186
BsiHKAI G_wGCw'C
Cuts at: 3285 3370 4441 3285
Size: 85 1071 4068
Fragments arranged by size:
4068 1071 85
BsmI GAATG_Cn'
Cuts at: 2149 2465 2735 2828 2149
Size: 316 270 93 4545
Fragments arranged by size:
4545 316 270 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2174 2543 3285 3370 4441 1391
Size: 399 319 65 369 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 369 319 85 65
BspEI T'CCGG_A
Cuts at: 4887 4887
Size: 5224
BspHI T'CATG_A
Cuts at: 3113 4843 3113
Size: 1730 3494
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2285 2579 844
Size: 1441 294 3489
Fragments arranged by size:
3489 1441 294
BsrBI CCG'CTC
Cuts at: 1394 1823 3111 1394
Size: 429 1288 3507
Fragments arranged by size:
3507 1288 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2237 3719 3893 65
Size: 1183 989 1482 174 1396
Fragments arranged by size:
1482 1396 1183 989 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2086 3868 4055 1700
Size: 110 276 1782 187 2869
Fragments arranged by size:
2869 1782 276 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5224
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5224
BssSI C'ACGA_G
Cuts at: 3284 4296 3284
Size: 1012 4212
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5224
BstBI TT'CG_AA
Cuts at: 4028 4028
Size: 5224
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5224
BstXI CCAn_nnnn'nTGG
Cuts at: 2116 2116
Size: 5224
BstYI r'GATC_y
Cuts at: 1972 2554 2921 3306 3323 4775 4861 4873 5219
Size: 582 367 385 17 1452 86 12 346
Cuts at: 5219 1972
Size: 1977
Fragments arranged by size:
1977 1452 582 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2073 2364 513
Size: 552 939 69 291 3373
Fragments arranged by size:
3373 939 552 291 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2493 487
Size: 683 56 1267 3218
Fragments arranged by size:
3218 1267 683 56
BtsI GCAGTG_nn'
Cuts at: 2451 2740 3534 3554 2451
Size: 289 794 20 4121
Fragments arranged by size:
4121 794 289 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5224
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5189
DraI TTT'AAA
Cuts at: 2528 2528
Size: 5224
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4231 809
Size: 507 2915 1802
Fragments arranged by size:
2915 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2499 2671 3560
Size: 54 1425 50 275 18 669 172 889
Cuts at: 3560 5189 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 669 275 172 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2671 1537
Size: 275 859 4090
Fragments arranged by size:
4090 859 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3154 1353
Size: 1801 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2105 3811 4185 4331 387
Size: 1064 138 516 1706 374 146 1280
Fragments arranged by size:
1706 1280 1064 516 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2308 3287 3884 4671 4939 1239
Size: 393 676 979 597 787 268 1524
Fragments arranged by size:
1524 979 787 676 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2292 2292
Size: 5224
EcoRI G'AATT_C
Cuts at: 2533 2911 2533
Size: 378 4846
FspI TGC'GCA
Cuts at: 3730 3730
Size: 5224
HaeII r_GCGC'y
Cuts at: 1678 2015 2081 2349 4371 1678
Size: 337 66 268 2022 2531
Fragments arranged by size:
2531 2022 337 268 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2084 2116 3865 3897 3939
Size: 32 135 32 512 32 1749 32 42
Cuts at: 3939 3971 1373
Size: 32 2626
Fragments arranged by size:
2626 1749 512 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2568 2814 669
Size: 988 281 630 246 3079
Fragments arranged by size:
3079 988 630 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5224
HpaI GTT'AAC
Cuts at: 2814 2814
Size: 5224
KasI G'GCGC_C
Cuts at: 2011 2011
Size: 5224
KpnI G_GTAC'C
Cuts at: 2549 2549
Size: 5224
MfeI C'AATT_G
Cuts at: 2823 2823
Size: 5224
MluI A'CGCG_T
Cuts at: 5100 5100
Size: 5224
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4338 4522 1305
Size: 3033 184 2007
Fragments arranged by size:
3033 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2501 10
Size: 54 1425 343 669 2733
Fragments arranged by size:
2733 1425 669 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2114 3182 3541 3700 518
Size: 648 948 1068 359 159 2042
Fragments arranged by size:
2042 1068 948 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2019 2640 3317 4465 4710 1606
Size: 413 621 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 621 413 245
NaeI GCC'GGC
Cuts at: 2088 2088
Size: 5224
NarI GG'CG_CC
Cuts at: 2012 2012
Size: 5224
NcoI C'CATG_G
Cuts at: 513 1065 2004 2364 513
Size: 552 939 360 3373
Fragments arranged by size:
3373 939 552 360
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5224
NgoMIV G'CCGG_C
Cuts at: 2086 2086
Size: 5224
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5224
NotI GC'GGCC_GC
Cuts at: 2671 2671
Size: 5224
NspI r_CATG'y
Cuts at: 1132 1229 2582 4127 1132
Size: 97 1353 1545 2229
Fragments arranged by size:
2229 1545 1353 97
PasI CC'CwG_GG
Cuts at: 1348 2296 1348
Size: 948 4276
PciI A'CATG_T
Cuts at: 4123 4123
Size: 5224
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2167 1108
Size: 1059 4165
PmeI GTTT'AAAC
Cuts at: 2528 2528
Size: 5224
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3293 3325 4806 4838 3293
Size: 32 1481 32 3679
Fragments arranged by size:
3679 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5224
PsiI TTA'TAA
Cuts at: 2794 2794
Size: 5224
PspOMI G'GGCC_C
Cuts at: 2170 2170
Size: 5224
PstI C_TGCA'G
Cuts at: 830 2576 830
Size: 1746 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5224
SalI G'TCGA_C
Cuts at: 1936 2566 1936
Size: 630 4594
SbfI CC_TGCA'GG
Cuts at: 2576 2576
Size: 5224
ScaI AGT'ACT
Cuts at: 3472 5184 3472
Size: 1712 3512
SfcI C'TryA_G
Cuts at: 826 1045 2572 3707 4388 4579 826
Size: 219 1527 1135 681 191 1471
Fragments arranged by size:
1527 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2013 2013
Size: 5224
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5224
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5224
SmaI CCC'GGG
Cuts at: 1872 2175 2461 2551 1872
Size: 303 286 90 4545
Fragments arranged by size:
4545 303 286 90
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2211 3328 4229 4491 820
Size: 197 926 268 1117 901 262 1553
Fragments arranged by size:
1553 1117 926 901 268 262 197
SnaBI TAC'GTA
Cuts at: 493 3015 493
Size: 2522 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5224
SphI G_CATG'C
Cuts at: 1132 2582 1132
Size: 1450 3774
SspI AAT'ATT
Cuts at: 5 52 3148 5
Size: 47 3096 2081
Fragments arranged by size:
3096 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5224
StyI C'CwwG_G
Cuts at: 513 1065 2004 2065 2364 2502 2663 513
Size: 552 939 61 299 138 161 3074
Fragments arranged by size:
3074 939 552 299 161 138 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3251 3268 3421 3606 4051 325
Size: 1255 1671 17 153 185 445 1498
Fragments arranged by size:
1671 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2313 3470 5182
Size: 274 80 33 51 1443 336 1157 1712
Cuts at: 5182 96
Size: 138
Fragments arranged by size:
1712 1443 1157 336 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5184 5216 5184
Size: 32 5192
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5224
XbaI T'CTAG_A
Cuts at: 2560 2560
Size: 5224
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2443 2443
Size: 5224
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5224
XmaI C'CCGG_G
Cuts at: 1870 2173 2459 2549 1870
Size: 303 286 90 4545
Fragments arranged by size:
4545 303 286 90
XmnI GAAnn'nnTTC
Cuts at: 3353 3353
Size: 5224
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3032 276
Size: 53 83 186 936 1498 2468
Fragments arranged by size:
2468 1498 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SrfI SwaI