Restriction map for
pFN21AE1656
(Circular) (Six-base) MAPSORT of: pFN21AE1656.seq Check: 6796 from: 1 to: 5224
pFN21AE1656 5224 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3034 278
Size: 53 83 186 936 1498 2468
Fragments arranged by size:
2468 1498 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2567 1937
Size: 630 4594
Acc65I G'GTAC_C
Cuts at: 2545 2545
Size: 5224
AclI AA'CG_TT
Cuts at: 1563 3351 3724 1563
Size: 1788 373 3063
Fragments arranged by size:
3063 1788 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2135 3287 4671 4939 1632
Size: 503 1152 1384 268 1917
Fragments arranged by size:
1917 1384 1152 503 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5027
AflIII A'CryG_T
Cuts at: 1203 4123 5100 1203
Size: 2920 977 1327
Fragments arranged by size:
2920 1327 977
AgeI A'CCGG_T
Cuts at: 4055 4055
Size: 5224
AhdI GACnn_n'nnGTC
Cuts at: 3953 3953
Size: 5224
AleI CACnn'nnGTG
Cuts at: 1166 2138 2198 1166
Size: 972 60 4192
Fragments arranged by size:
4192 972 60
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5224
AlwNI CAG_nnn'CTG
Cuts at: 2476 2967 4539 2476
Size: 491 1572 3161
Fragments arranged by size:
3161 1572 491
ApaLI G'TGCA_C
Cuts at: 2309 3281 4437 2309
Size: 972 1156 3096
Fragments arranged by size:
3096 1156 972
ApoI r'AATT_y
Cuts at: 1493 2533 2759 2911 5020 1493
Size: 1040 226 152 2109 1697
Fragments arranged by size:
2109 1697 1040 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3778 160
Size: 872 2746 1606
Fragments arranged by size:
2746 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5224
AvaI C'yCGr_G
Cuts at: 1870 1943 2549 1870
Size: 73 606 4545
Fragments arranged by size:
4545 606 73
BamHI G'GATC_C
Cuts at: 2554 2921 2554
Size: 367 4857
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2224 2545 4000 618
Size: 325 217 627 437 321 1455 1842
Fragments arranged by size:
1842 1455 627 437 325 321 217
BanII G_rGCy'C
Cuts at: 1391 2338 2543 1391
Size: 947 205 4072
Fragments arranged by size:
4072 947 205
BbeI G_GCGC'C
Cuts at: 2228 2228
Size: 5224
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2256 928
Size: 971 357 3896
Fragments arranged by size:
3896 971 357
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3415 3449 1903
Size: 34 1478 34 3678
Fragments arranged by size:
3678 1478 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3116 4326 3116
Size: 1210 4014
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5224
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3835 136
Size: 107 122 71 631 605 2163 1525
Fragments arranged by size:
2163 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5219 5219
Size: 5224
BlpI GC'TnA_GC
Cuts at: 2093 2258 2641 2093
Size: 165 383 4676
Fragments arranged by size:
4676 383 165
Bme1580I G_kGCm'C
Cuts at: 1790 2313 3285 4441 1790
Size: 523 972 1156 2573
Fragments arranged by size:
2573 1156 972 523
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5224
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2955 3913 442
Size: 416 53 83 399 24 1538 958 1753
Fragments arranged by size:
1753 1538 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5224
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2198 2322 2471 3884 1239
Size: 959 124 149 1413 2579
Fragments arranged by size:
2579 1413 959 149 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3349 4214 4512 3349
Size: 865 298 4061
Fragments arranged by size:
4061 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3887 882
Size: 617 2388 2219
Fragments arranged by size:
2388 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3015 493
Size: 711 1811 2702
Fragments arranged by size:
2702 1811 711
BsaBI GATnn'nnATC
Cuts at: 5218 5218
Size: 5224
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2225 2388 3031 3413
Size: 53 83 186 936 692 163 643 382
Cuts at: 3413 4048 275
Size: 635 1451
Fragments arranged by size:
1451 936 692 643 635 382 186 163
83 53
BsaWI w'CCGG_w
Cuts at: 3657 4055 4329 4476 4887 3657
Size: 398 274 147 411 3994
Fragments arranged by size:
3994 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4999
Fragments arranged by size:
4999 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5224
BseYI C'CCAG_C
Cuts at: 4427 4923 4427
Size: 496 4728
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4651
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2674 3435 4463 1540
Size: 275 186 673 761 1028 2301
Fragments arranged by size:
2301 1028 761 673 275 186
BsiHKAI G_wGCw'C
Cuts at: 2313 3285 3370 4441 2313
Size: 972 85 1071 3096
Fragments arranged by size:
3096 1071 972 85
BsmI GAATG_Cn'
Cuts at: 2735 2828 2735
Size: 93 5131
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2313 2338 2543 3285 3370 4441 1391
Size: 399 523 25 205 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 523 399 205 85 25
BspEI T'CCGG_A
Cuts at: 4887 4887
Size: 5224
BspHI T'CATG_A
Cuts at: 3113 4843 3113
Size: 1730 3494
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2579 844
Size: 1735 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2370 3111 1394
Size: 429 547 741 3507
Fragments arranged by size:
3507 741 547 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3719 3893 65
Size: 1183 2471 174 1396
Fragments arranged by size:
2471 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2460 3868 4055 1700
Size: 110 650 1408 187 2869
Fragments arranged by size:
2869 1408 650 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5224
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5224
BssSI C'ACGA_G
Cuts at: 3284 4296 3284
Size: 1012 4212
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5224
BstBI TT'CG_AA
Cuts at: 4028 4028
Size: 5224
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5224
BstYI r'GATC_y
Cuts at: 1972 2554 2921 3306 3323 4775 4861 4873 5219
Size: 582 367 385 17 1452 86 12 346
Cuts at: 5219 1972
Size: 1977
Fragments arranged by size:
1977 1452 582 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2416 513
Size: 552 939 412 3321
Fragments arranged by size:
3321 939 552 412
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4485
Fragments arranged by size:
4485 683 56
BtsI GCAGTG_nn'
Cuts at: 2311 2740 3534 3554 2311
Size: 429 794 20 3981
Fragments arranged by size:
3981 794 429 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5224
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5189
DraI TTT'AAA
Cuts at: 2528 2528
Size: 5224
DraIII CAC_nnn'GTG
Cuts at: 2317 2317
Size: 5224
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4231 809
Size: 507 2915 1802
Fragments arranged by size:
2915 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2207 2671 3560
Size: 54 1425 50 275 18 377 464 889
Cuts at: 3560 5189 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 464 377 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2671 1537
Size: 275 859 4090
Fragments arranged by size:
4090 859 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3154 1353
Size: 1801 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2393 3811 4185 4331 387
Size: 1064 138 804 1418 374 146 1280
Fragments arranged by size:
1418 1280 1064 804 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2135 2198 2322 2471 3287 3884 4671
Size: 393 503 63 124 149 816 597 787
Cuts at: 4671 4939 1239
Size: 268 1524
Fragments arranged by size:
1524 816 787 597 503 393 268 149
124 63
EcoNI CCTnn'n_nnAGG
Cuts at: 2267 2267
Size: 5224
EcoO109I rG'GnC_Cy
Cuts at: 2110 2287 2110
Size: 177 5047
EcoRI G'AATT_C
Cuts at: 2533 2911 2533
Size: 378 4846
FspI TGC'GCA
Cuts at: 3730 3730
Size: 5224
HaeII r_GCGC'y
Cuts at: 1678 2228 2354 2416 4371 1678
Size: 550 126 62 1955 2531
Fragments arranged by size:
2531 1955 550 126 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3865 3897 3939 3971 1373
Size: 32 135 32 2293 32 42 32 2626
Fragments arranged by size:
2626 2293 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2568 2814 669
Size: 988 281 630 246 3079
Fragments arranged by size:
3079 988 630 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5224
HpaI GTT'AAC
Cuts at: 2814 2814
Size: 5224
KasI G'GCGC_C
Cuts at: 2224 2224
Size: 5224
KpnI G_GTAC'C
Cuts at: 2549 2549
Size: 5224
MfeI C'AATT_G
Cuts at: 2823 2823
Size: 5224
MluI A'CGCG_T
Cuts at: 5100 5100
Size: 5224
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4338 4522 1305
Size: 3033 184 2007
Fragments arranged by size:
3033 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3402
Fragments arranged by size:
3402 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2138 2198 3182 3541 3700 518
Size: 648 972 60 984 359 159 2042
Fragments arranged by size:
2042 984 972 648 359 159 60
MspA1I CmG'CkG
Cuts at: 1606 2176 2298 2418 2640 3317 4465 4710 1606
Size: 570 122 120 222 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 570 245 222 122 120
NarI GG'CG_CC
Cuts at: 2225 2225
Size: 5224
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3733
Fragments arranged by size:
3733 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5224
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5224
NotI GC'GGCC_GC
Cuts at: 2671 2671
Size: 5224
NspI r_CATG'y
Cuts at: 1132 1229 2056 2582 4127 1132
Size: 97 827 526 1545 2229
Fragments arranged by size:
2229 1545 827 526 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5224
PciI A'CATG_T
Cuts at: 4123 4123
Size: 5224
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5224
PmeI GTTT'AAAC
Cuts at: 2528 2528
Size: 5224
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3293 3325 4806 4838 3293
Size: 32 1481 32 3679
Fragments arranged by size:
3679 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2110 2110
Size: 5224
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5224
PsiI TTA'TAA
Cuts at: 2794 2794
Size: 5224
PstI C_TGCA'G
Cuts at: 830 2576 830
Size: 1746 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5224
PvuII CAG'CTG
Cuts at: 2298 2298
Size: 5224
SacII CC_GC'GG
Cuts at: 2419 2419
Size: 5224
SalI G'TCGA_C
Cuts at: 1936 2566 1936
Size: 630 4594
SbfI CC_TGCA'GG
Cuts at: 2576 2576
Size: 5224
ScaI AGT'ACT
Cuts at: 3472 5184 3472
Size: 1712 3512
SfcI C'TryA_G
Cuts at: 826 1045 2572 3707 4388 4579 826
Size: 219 1527 1135 681 191 1471
Fragments arranged by size:
1527 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2226 2226
Size: 5224
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5224
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5224
SmaI CCC'GGG
Cuts at: 1872 2551 1872
Size: 679 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3328 4229 4491 820
Size: 197 926 1385 901 262 1553
Fragments arranged by size:
1553 1385 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3015 493
Size: 2522 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5224
SphI G_CATG'C
Cuts at: 1132 2582 1132
Size: 1450 3774
SspI AAT'ATT
Cuts at: 5 52 3148 5
Size: 47 3096 2081
Fragments arranged by size:
3096 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5224
StyI C'CwwG_G
Cuts at: 513 1065 2004 2663 513
Size: 552 939 659 3074
Fragments arranged by size:
3074 939 659 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2084 3251 3268 3421 3606 4051 325
Size: 1255 504 1167 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1167 504 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3470 5182 96
Size: 274 80 33 51 1443 1493 1712 138
Fragments arranged by size:
1712 1493 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5184 5216 5184
Size: 32 5192
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5224
XbaI T'CTAG_A
Cuts at: 2560 2560
Size: 5224
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5224
XmaI C'CCGG_G
Cuts at: 1870 2549 1870
Size: 679 4545
XmnI GAAnn'nnTTC
Cuts at: 3353 3353
Size: 5224
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3032 276
Size: 53 83 186 936 1498 2468
Fragments arranged by size:
2468 1498 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI