Restriction map for
pFN21AE1612
(Circular) (Six-base) MAPSORT of: pFN21AE1612.seq Check: 2039 from: 1 to: 5062
pFN21AE1612 5062 bp
With 182 enzymes: SgfI *
August 16, 2010 14:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2366 2366
Size: 5062
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2872 278
Size: 53 83 186 936 1336 2468
Fragments arranged by size:
2468 1336 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2405 1937
Size: 468 4594
Acc65I G'GTAC_C
Cuts at: 2383 2383
Size: 5062
AclI AA'CG_TT
Cuts at: 1563 3189 3562 1563
Size: 1626 373 3063
Fragments arranged by size:
3063 1626 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3125 4509 4777 1632
Size: 1493 1384 268 1917
Fragments arranged by size:
1917 1493 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4865
AflIII A'CryG_T
Cuts at: 1203 3961 4938 1203
Size: 2758 977 1327
Fragments arranged by size:
2758 1327 977
AgeI A'CCGG_T
Cuts at: 3893 3893
Size: 5062
AhdI GACnn_n'nnGTC
Cuts at: 3791 3791
Size: 5062
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5062
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5062
AlwNI CAG_nnn'CTG
Cuts at: 2315 2805 4377 2315
Size: 490 1572 3000
Fragments arranged by size:
3000 1572 490
ApaI G_GGCC'C
Cuts at: 2327 2327
Size: 5062
ApaLI G'TGCA_C
Cuts at: 3119 4275 3119
Size: 1156 3906
ApoI r'AATT_y
Cuts at: 1493 2127 2371 2597 2749 4858 1493
Size: 634 244 226 152 2109 1697
Fragments arranged by size:
2109 1697 634 244 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3616 160
Size: 872 2584 1606
Fragments arranged by size:
2584 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5062
AvaI C'yCGr_G
Cuts at: 1870 1943 2387 1870
Size: 73 444 4545
Fragments arranged by size:
4545 444 73
AvrII C'CTAG_G
Cuts at: 2297 2297
Size: 5062
BamHI G'GATC_C
Cuts at: 2392 2759 2392
Size: 367 4695
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2383 3838 618
Size: 325 217 627 596 1455 1842
Fragments arranged by size:
1842 1455 627 596 325 217
BanII G_rGCy'C
Cuts at: 1391 2081 2327 2381 1391
Size: 690 246 54 4072
Fragments arranged by size:
4072 690 246 54
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4091
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3253 3287 1903
Size: 34 1316 34 3678
Fragments arranged by size:
3678 1316 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2954 4164 2954
Size: 1210 3852
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5062
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3673 136
Size: 107 122 71 631 605 2001 1525
Fragments arranged by size:
2001 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5057 5057
Size: 5062
BlpI GC'TnA_GC
Cuts at: 2479 2479
Size: 5062
Bme1580I G_kGCm'C
Cuts at: 1790 2059 2327 3123 4279 1790
Size: 269 268 796 1156 2573
Fragments arranged by size:
2573 1156 796 269 268
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5062
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2793 3751 442
Size: 416 53 83 399 24 1376 958 1753
Fragments arranged by size:
1753 1376 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5062
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3722 1239
Size: 2483 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2165 3187 4052 4350 2165
Size: 1022 865 298 2877
Fragments arranged by size:
2877 1022 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3725 882
Size: 617 2226 2219
Fragments arranged by size:
2226 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2853 493
Size: 711 1649 2702
Fragments arranged by size:
2702 1649 711
BsaBI GATnn'nnATC
Cuts at: 5056 5056
Size: 5062
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2274 2869 3251 3886
Size: 53 83 186 936 741 595 382 635
Cuts at: 3886 275
Size: 1451
Fragments arranged by size:
1451 936 741 635 595 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3495 3893 4167 4314 4725 3495
Size: 398 274 147 411 3832
Fragments arranged by size:
3832 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4837
Fragments arranged by size:
4837 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5062
BseYI C'CCAG_C
Cuts at: 4265 4761 4265
Size: 496 4566
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4489
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2512 3273 4301 1540
Size: 275 186 511 761 1028 2301
Fragments arranged by size:
2301 1028 761 511 275 186
BsiHKAI G_wGCw'C
Cuts at: 2174 3123 3208 4279 2174
Size: 949 85 1071 2957
Fragments arranged by size:
2957 1071 949 85
BsmI GAATG_Cn'
Cuts at: 2573 2666 2573
Size: 93 4969
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2059 2081 2174 2327 2381 3123 3208
Size: 399 269 22 93 153 54 742 85
Cuts at: 3208 4279 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 269 153 93 85
54 22
BspEI T'CCGG_A
Cuts at: 4725 4725
Size: 5062
BspHI T'CATG_A
Cuts at: 2951 4681 2951
Size: 1730 3332
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2366 2417 844
Size: 1522 51 3489
Fragments arranged by size:
3489 1522 51
BsrBI CCG'CTC
Cuts at: 1394 1823 2949 1394
Size: 429 1126 3507
Fragments arranged by size:
3507 1126 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3557 3731 65
Size: 1183 2309 174 1396
Fragments arranged by size:
2309 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3706 3893 1700
Size: 110 1896 187 2869
Fragments arranged by size:
2869 1896 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5062
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5062
BssSI C'ACGA_G
Cuts at: 3122 4134 3122
Size: 1012 4050
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5062
BstBI TT'CG_AA
Cuts at: 3866 3866
Size: 5062
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5062
BstXI CCAn_nnnn'nTGG
Cuts at: 2311 2311
Size: 5062
BstYI r'GATC_y
Cuts at: 1972 2392 2759 3144 3161 4613 4699 4711 5057
Size: 420 367 385 17 1452 86 12 346
Cuts at: 5057 1972
Size: 1977
Fragments arranged by size:
1977 1452 420 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3571
Fragments arranged by size:
3571 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4323
Fragments arranged by size:
4323 683 56
BtsI GCAGTG_nn'
Cuts at: 2316 2578 3372 3392 2316
Size: 262 794 20 3986
Fragments arranged by size:
3986 794 262 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5062
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5027
DraI TTT'AAA
Cuts at: 2366 2366
Size: 5062
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4069 809
Size: 507 2753 1802
Fragments arranged by size:
2753 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2509 3398 5027
Size: 54 1425 50 275 18 679 889 1629
Cuts at: 5027 8
Size: 43
Fragments arranged by size:
1629 1425 889 679 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2509 1537
Size: 275 697 4090
Fragments arranged by size:
4090 697 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2992 1353
Size: 1639 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3649 4023 4169 387
Size: 1064 138 2060 374 146 1280
Fragments arranged by size:
2060 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3125 3722 4509 4777 1239
Size: 393 1493 597 787 268 1524
Fragments arranged by size:
1524 1493 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2323 2324 2323
Size: 1 5061
EcoRI G'AATT_C
Cuts at: 2127 2371 2749 2127
Size: 244 378 4440
Fragments arranged by size:
4440 378 244
EcoRV GAT'ATC
Cuts at: 2339 2339
Size: 5062
FspI TGC'GCA
Cuts at: 3568 3568
Size: 5062
HaeII r_GCGC'y
Cuts at: 1678 4209 1678
Size: 2531 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3703 3735 3777 3809 1373
Size: 32 135 32 2131 32 42 32 2626
Fragments arranged by size:
2626 2131 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2406 2652 669
Size: 988 281 468 246 3079
Fragments arranged by size:
3079 988 468 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5062
HpaI GTT'AAC
Cuts at: 2652 2652
Size: 5062
KpnI G_GTAC'C
Cuts at: 2387 2387
Size: 5062
MfeI C'AATT_G
Cuts at: 2661 2661
Size: 5062
MluI A'CGCG_T
Cuts at: 4938 4938
Size: 5062
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4176 4360 1305
Size: 2871 184 2007
Fragments arranged by size:
2871 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3240
Fragments arranged by size:
3240 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3020 3379 3538 518
Size: 648 1854 359 159 2042
Fragments arranged by size:
2042 1854 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2158 2478 3155 4303 4548 1606
Size: 552 320 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 552 320 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3571
Fragments arranged by size:
3571 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5062
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5062
NotI GC'GGCC_GC
Cuts at: 2509 2509
Size: 5062
NspI r_CATG'y
Cuts at: 1132 1229 2110 2178 2420 3965 1132
Size: 97 881 68 242 1545 2229
Fragments arranged by size:
2229 1545 881 242 97 68
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5062
PciI A'CATG_T
Cuts at: 3961 3961
Size: 5062
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5062
PmeI GTTT'AAAC
Cuts at: 2366 2366
Size: 5062
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3131 3163 4644 4676 3131
Size: 32 1481 32 3517
Fragments arranged by size:
3517 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5062
PsiI TTA'TAA
Cuts at: 2632 2632
Size: 5062
PspOMI G'GGCC_C
Cuts at: 2323 2323
Size: 5062
PstI C_TGCA'G
Cuts at: 830 2414 830
Size: 1584 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5062
PvuII CAG'CTG
Cuts at: 2158 2158
Size: 5062
SalI G'TCGA_C
Cuts at: 1936 2404 1936
Size: 468 4594
SbfI CC_TGCA'GG
Cuts at: 2414 2414
Size: 5062
ScaI AGT'ACT
Cuts at: 3310 5022 3310
Size: 1712 3350
SfcI C'TryA_G
Cuts at: 826 1045 2410 3545 4226 4417 826
Size: 219 1365 1135 681 191 1471
Fragments arranged by size:
1471 1365 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5062
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5062
SmaI CCC'GGG
Cuts at: 1872 2389 1872
Size: 517 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2180 3166 4067 4329 820
Size: 197 926 237 986 901 262 1553
Fragments arranged by size:
1553 986 926 901 262 237 197
SnaBI TAC'GTA
Cuts at: 493 2853 493
Size: 2360 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5062
SphI G_CATG'C
Cuts at: 1132 2420 1132
Size: 1288 3774
SspI AAT'ATT
Cuts at: 5 52 2986 5
Size: 47 2934 2081
Fragments arranged by size:
2934 2081 47
StuI AGG'CCT
Cuts at: 1517 2013 1517
Size: 496 4566
StyI C'CwwG_G
Cuts at: 513 1065 2004 2044 2297 2501 513
Size: 552 939 40 253 204 3074
Fragments arranged by size:
3074 939 552 253 204 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3089 3106 3259 3444 3889 325
Size: 1255 1509 17 153 185 445 1498
Fragments arranged by size:
1509 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3308 5020 96
Size: 274 80 33 51 1443 1331 1712 138
Fragments arranged by size:
1712 1443 1331 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5022 5054 5022
Size: 32 5030
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5062
XbaI T'CTAG_A
Cuts at: 2398 2398
Size: 5062
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2222 2339 2222
Size: 117 4945
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5062
XmaI C'CCGG_G
Cuts at: 1870 2387 1870
Size: 517 4545
XmnI GAAnn'nnTTC
Cuts at: 3191 3191
Size: 5062
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2870 276
Size: 53 83 186 936 1336 2468
Fragments arranged by size:
2468 1336 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI