Restriction map for
pFN21AE1608
(Circular) (Six-base) MAPSORT of: pFN21AE1608.seq Check: 7606 from: 1 to: 5047
pFN21AE1608 5047 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2857 278
Size: 53 83 186 936 1321 2468
Fragments arranged by size:
2468 1321 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2390 1937
Size: 453 4594
Acc65I G'GTAC_C
Cuts at: 2368 2368
Size: 5047
AclI AA'CG_TT
Cuts at: 1563 3174 3547 1563
Size: 1611 373 3063
Fragments arranged by size:
3063 1611 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3110 4494 4762 1632
Size: 1478 1384 268 1917
Fragments arranged by size:
1917 1478 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4850
AflIII A'CryG_T
Cuts at: 1203 3946 4923 1203
Size: 2743 977 1327
Fragments arranged by size:
2743 1327 977
AgeI A'CCGG_T
Cuts at: 3878 3878
Size: 5047
AhdI GACnn_n'nnGTC
Cuts at: 3776 3776
Size: 5047
AleI CACnn'nnGTG
Cuts at: 1166 2292 1166
Size: 1126 3921
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5047
AlwNI CAG_nnn'CTG
Cuts at: 2790 4362 2790
Size: 1572 3475
ApaI G_GGCC'C
Cuts at: 2306 2306
Size: 5047
ApaLI G'TGCA_C
Cuts at: 3104 4260 3104
Size: 1156 3891
ApoI r'AATT_y
Cuts at: 1493 2111 2143 2356 2582 2734 4843 1493
Size: 618 32 213 226 152 2109 1697
Fragments arranged by size:
2109 1697 618 226 213 152 32
AseI AT'TA_AT
Cuts at: 160 1032 3601 160
Size: 872 2569 1606
Fragments arranged by size:
2569 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5047
AvaI C'yCGr_G
Cuts at: 1870 1943 2330 2372 1870
Size: 73 387 42 4545
Fragments arranged by size:
4545 387 73 42
BamHI G'GATC_C
Cuts at: 2377 2744 2377
Size: 367 4680
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2085 2339 2368 3823 618
Size: 325 217 627 298 254 29 1455 1842
Fragments arranged by size:
1842 1455 627 325 298 254 217 29
BanII G_rGCy'C
Cuts at: 1391 2306 2366 1391
Size: 915 60 4072
Fragments arranged by size:
4072 915 60
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4076
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3238 3272 1903
Size: 34 1301 34 3678
Fragments arranged by size:
3678 1301 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2939 4149 2939
Size: 1210 3837
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5047
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3658 136
Size: 107 122 71 631 605 1986 1525
Fragments arranged by size:
1986 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2134 5042 2134
Size: 2908 2139
BlpI GC'TnA_GC
Cuts at: 2225 2464 2225
Size: 239 4808
Bme1580I G_kGCm'C
Cuts at: 1790 2088 2306 2342 3108 4264 1790
Size: 298 218 36 766 1156 2573
Fragments arranged by size:
2573 1156 766 298 218 36
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5047
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2778 3736 442
Size: 416 53 83 399 24 1361 958 1753
Fragments arranged by size:
1753 1361 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5047
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3707 1239
Size: 2468 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2135 3172 4037 4335 2135
Size: 1037 865 298 2847
Fragments arranged by size:
2847 1037 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2036 3710 882
Size: 617 537 1674 2219
Fragments arranged by size:
2219 1674 617 537
BsaAI yAC'GTr
Cuts at: 493 1204 2838 493
Size: 711 1634 2702
Fragments arranged by size:
2702 1634 711
BsaBI GATnn'nnATC
Cuts at: 5041 5041
Size: 5047
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2854 3236 3871 275
Size: 53 83 186 936 1321 382 635 1451
Fragments arranged by size:
1451 1321 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3480 3878 4152 4299 4710 3480
Size: 398 274 147 411 3817
Fragments arranged by size:
3817 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4822
Fragments arranged by size:
4822 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2249 1186
Size: 1063 3984
BseYI C'CCAG_C
Cuts at: 2080 4250 4746 2080
Size: 2170 496 2381
Fragments arranged by size:
2381 2170 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4474
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2497 3258 4286 1540
Size: 275 186 496 761 1028 2301
Fragments arranged by size:
2301 1028 761 496 275 186
BsiHKAI G_wGCw'C
Cuts at: 3108 3193 4264 3108
Size: 85 1071 3891
Fragments arranged by size:
3891 1071 85
BsmI GAATG_Cn'
Cuts at: 2558 2651 2558
Size: 93 4954
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2306 2342 2366 3108 3193 4264
Size: 399 298 218 36 24 742 85 1071
Cuts at: 4264 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 298 218 85 36
24
BspEI T'CCGG_A
Cuts at: 4710 4710
Size: 5047
BspHI T'CATG_A
Cuts at: 2936 4666 2936
Size: 1730 3317
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2402 844
Size: 1558 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2934 1394
Size: 429 1111 3507
Fragments arranged by size:
3507 1111 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3542 3716 65
Size: 1183 2294 174 1396
Fragments arranged by size:
2294 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3691 3878 1700
Size: 110 1881 187 2869
Fragments arranged by size:
2869 1881 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5047
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5047
BssSI C'ACGA_G
Cuts at: 2276 2288 3107 4119 2276
Size: 12 819 1012 3204
Fragments arranged by size:
3204 1012 819 12
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5047
BstBI TT'CG_AA
Cuts at: 3851 3851
Size: 5047
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5047
BstYI r'GATC_y
Cuts at: 1972 2134 2377 2744 3129 3146 4598 4684 4696
Size: 162 243 367 385 17 1452 86 12
Cuts at: 4696 5042 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 243 162 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4495
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4308
Fragments arranged by size:
4308 683 56
BtsI GCAGTG_nn'
Cuts at: 2563 3357 3377 2563
Size: 794 20 4233
Fragments arranged by size:
4233 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5047
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5012
DraI TTT'AAA
Cuts at: 2351 2351
Size: 5047
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4054 809
Size: 507 2738 1802
Fragments arranged by size:
2738 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2308 2494 3383
Size: 54 1425 50 275 18 478 186 889
Cuts at: 3383 5012 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 478 275 186 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2494 1537
Size: 275 682 4090
Fragments arranged by size:
4090 682 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2977 1353
Size: 1624 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3634 4008 4154 387
Size: 1064 138 2045 374 146 1280
Fragments arranged by size:
2045 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3110 3707 4494 4762 1239
Size: 393 1478 597 787 268 1524
Fragments arranged by size:
1524 1478 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2102 2333 2102
Size: 231 4816
EcoO109I rG'GnC_Cy
Cuts at: 2197 2302 2303 2197
Size: 105 1 4941
Fragments arranged by size:
4941 105 1
EcoRI G'AATT_C
Cuts at: 2111 2356 2734 2111
Size: 245 378 4424
Fragments arranged by size:
4424 378 245
FspI TGC'GCA
Cuts at: 3553 3553
Size: 5047
HaeII r_GCGC'y
Cuts at: 1678 4194 1678
Size: 2516 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3688 3720 3762 3794 1373
Size: 32 135 32 2116 32 42 32 2626
Fragments arranged by size:
2626 2116 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2391 2637 669
Size: 988 281 453 246 3079
Fragments arranged by size:
3079 988 453 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5047
HpaI GTT'AAC
Cuts at: 2637 2637
Size: 5047
KpnI G_GTAC'C
Cuts at: 2372 2372
Size: 5047
MfeI C'AATT_G
Cuts at: 2646 2646
Size: 5047
MluI A'CGCG_T
Cuts at: 4923 4923
Size: 5047
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4161 4345 1305
Size: 2856 184 2007
Fragments arranged by size:
2856 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2310 10
Size: 54 1425 343 478 2747
Fragments arranged by size:
2747 1425 478 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2249 2292 3005 3364 3523 518
Size: 648 1083 43 713 359 159 2042
Fragments arranged by size:
2042 1083 713 648 359 159 43
MspA1I CmG'CkG
Cuts at: 1606 2026 2224 2463 3140 4288 4533 1606
Size: 420 198 239 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 420 245 239 198
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4495
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5047
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5047
NotI GC'GGCC_GC
Cuts at: 2494 2494
Size: 5047
NspI r_CATG'y
Cuts at: 1132 1229 2405 3950 1132
Size: 97 1176 1545 2229
Fragments arranged by size:
2229 1545 1176 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5047
PciI A'CATG_T
Cuts at: 3946 3946
Size: 5047
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5047
PmeI GTTT'AAAC
Cuts at: 2351 2351
Size: 5047
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3116 3148 4629 4661 3116
Size: 32 1481 32 3502
Fragments arranged by size:
3502 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2197 2197
Size: 5047
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5047
PsiI TTA'TAA
Cuts at: 2617 2617
Size: 5047
PspOMI G'GGCC_C
Cuts at: 2302 2302
Size: 5047
PstI C_TGCA'G
Cuts at: 830 2399 830
Size: 1569 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5047
PvuII CAG'CTG
Cuts at: 2026 2224 2026
Size: 198 4849
SalI G'TCGA_C
Cuts at: 1936 2389 1936
Size: 453 4594
SbfI CC_TGCA'GG
Cuts at: 2399 2399
Size: 5047
ScaI AGT'ACT
Cuts at: 3295 5007 3295
Size: 1712 3335
SfcI C'TryA_G
Cuts at: 826 1045 2395 3530 4211 4402 826
Size: 219 1350 1135 681 191 1471
Fragments arranged by size:
1471 1350 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5047
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5047
SmaI CCC'GGG
Cuts at: 1872 2374 1872
Size: 502 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2150 3151 4052 4314 820
Size: 197 926 207 1001 901 262 1553
Fragments arranged by size:
1553 1001 926 901 262 207 197
SnaBI TAC'GTA
Cuts at: 493 2838 493
Size: 2345 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5047
SphI G_CATG'C
Cuts at: 1132 2405 1132
Size: 1273 3774
SspI AAT'ATT
Cuts at: 5 52 2971 5
Size: 47 2919 2081
Fragments arranged by size:
2919 2081 47
StuI AGG'CCT
Cuts at: 1517 2328 1517
Size: 811 4236
StyI C'CwwG_G
Cuts at: 513 1065 2486 513
Size: 552 1421 3074
Fragments arranged by size:
3074 1421 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2103 3074 3091 3244 3429 3874 325
Size: 1255 523 971 17 153 185 445 1498
Fragments arranged by size:
1498 1255 971 523 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3293 5005 96
Size: 274 80 33 51 1443 1316 1712 138
Fragments arranged by size:
1712 1443 1316 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5007 5039 5007
Size: 32 5015
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5047
XbaI T'CTAG_A
Cuts at: 2383 2383
Size: 5047
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5047
XmaI C'CCGG_G
Cuts at: 1870 2372 1870
Size: 502 4545
XmnI GAAnn'nnTTC
Cuts at: 3176 3176
Size: 5047
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2855 276
Size: 53 83 186 936 1321 2468
Fragments arranged by size:
2468 1321 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI